BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0059.p (127 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_694020.1| glutamate synthase [NADPH] small subunit [Ocean... 173 1e-42 ref|NP_242595.1| glutamate synthase (small subunit) [Bacillus ha... 170 1e-41 dbj|BAA12742.1| small subunit of NADH-dependent glutamate syntha... 157 7e-38 ref|NP_389726.1| glutamate synthase (small subunit) [Bacillus su... 153 9e-37 ref|ZP_00098864.1| COG0493: NADPH-dependent glutamate synthase b... 146 2e-34 >ref|NP_694020.1| glutamate synthase [NADPH] small subunit [Oceanobacillus iheyensis HTE831] dbj|BAC15054.1| glutamate synthase [NADPH] small subunit [Oceanobacillus iheyensis HTE831] Length = 494 Score = 173 bits (439), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 81/125 (64%), Positives = 102/125 (81%), Gaps = 1/125 (0%) Query: 2 TTKFVGDEEGNLKELHTVQIQRMVDETGRKIYQPIPGTEAVYPAQLALIAIGFDGPEQDI 61 TTKFVGDE G++KE+HT+ ++ E G+KI + IPGTE V+PA L L+AIGF GP+QD+ Sbjct: 370 TTKFVGDENGHVKEVHTINVKTRY-EGGKKIREEIPGTEKVWPADLVLVAIGFQGPDQDL 428 Query: 62 IEELKLDTDRRSNVKARYGKFNTNVDKVFAAGDMRRGQSLVVWAINEGREAAREVDKYLM 121 IE L ++T RS VKA+YG+F TN+D VFAAGDMRRGQSL+VWAINEGR AARE D++LM Sbjct: 429 IESLDVETTTRSTVKAKYGEFQTNIDGVFAAGDMRRGQSLIVWAINEGRGAARECDRFLM 488 Query: 122 GSTVL 126 G T+L Sbjct: 489 GDTIL 493 >ref|NP_242595.1| glutamate synthase (small subunit) [Bacillus halodurans] pir||A83866 glutamate synthase (small subunit) gltB [imported] - Bacillus halodurans (strain C-125) dbj|BAB05448.1| glutamate synthase (small subunit) [Bacillus halodurans] Length = 495 Score = 170 bits (430), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 78/126 (61%), Positives = 100/126 (79%) Query: 1 MTTKFVGDEEGNLKELHTVQIQRMVDETGRKIYQPIPGTEAVYPAQLALIAIGFDGPEQD 60 MTTKFVGD EG++KE+HT+ ++ V G K IPGTE V+PA L +A+GF GPE+ Sbjct: 369 MTTKFVGDAEGHVKEIHTISVETTVHPDGSKTRVEIPGTEKVWPADLVFLAVGFTGPEEQ 428 Query: 61 IIEELKLDTDRRSNVKARYGKFNTNVDKVFAAGDMRRGQSLVVWAINEGREAAREVDKYL 120 ++ EL+L+TD RSN+KA YGK+ T+++ VFAAGD RRGQSLVVWAI+EGREAARE DKYL Sbjct: 429 LVRELELETDTRSNIKAEYGKYETSLEGVFAAGDNRRGQSLVVWAIHEGREAARECDKYL 488 Query: 121 MGSTVL 126 MG++ L Sbjct: 489 MGASNL 494 >dbj|BAA12742.1| small subunit of NADH-dependent glutamate synthase [Plectonema boryanum] Length = 492 Score = 157 bits (397), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 74/125 (59%), Positives = 95/125 (76%) Query: 2 TTKFVGDEEGNLKELHTVQIQRMVDETGRKIYQPIPGTEAVYPAQLALIAIGFDGPEQDI 61 T F GDE+GN+K +HTV++Q +E G+ I + IPGTE + PAQL L+A+GF GPEQ + Sbjct: 367 ATHFEGDEQGNVKAVHTVEVQWERNEQGQFIPKQIPGTEKIIPAQLVLLAMGFLGPEQPL 426 Query: 62 IEELKLDTDRRSNVKARYGKFNTNVDKVFAAGDMRRGQSLVVWAINEGREAAREVDKYLM 121 ++ + LD D RSN+K+ YG++ T++ KVFAAGD RRGQSLVVWA NEGR AARE D YLM Sbjct: 427 LDAMGLDRDARSNIKSEYGRYTTSIPKVFAAGDCRRGQSLVVWAFNEGRGAARECDLYLM 486 Query: 122 GSTVL 126 G T L Sbjct: 487 GETNL 491 >ref|NP_389726.1| glutamate synthase (small subunit) [Bacillus subtilis] pir||H69634 glutamate synthase (small subunit) gltB - Bacillus subtilis emb|CAB13727.1| glutamate synthase (small subunit) [Bacillus subtilis subsp. subtilis str. 168] Length = 493 Score = 153 bits (387), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 71/125 (56%), Positives = 98/125 (78%), Gaps = 1/125 (0%) Query: 2 TTKFVGDEEGNLKELHTVQIQRMVDETGRKIYQPIPGTEAVYPAQLALIAIGFDGPEQDI 61 TTK V D+ G LKELHT+Q++++ +E G+ ++ +PGTE V+PAQL IAIGF+G EQ + Sbjct: 369 TTKMVADKNGKLKELHTIQMEKVKNEHGKYEFRELPGTEKVWPAQLVFIAIGFEGTEQPL 428 Query: 62 IEELKLDTDRRSNVKARYGKFNTNVDKVFAAGDMRRGQSLVVWAINEGREAAREVDKYLM 121 +++ +++ + + A YG + TN+D VFAAGD RRGQSL+VWAINEGRE AREVD+YLM Sbjct: 429 LKQFGVNS-VNNKISAAYGDYQTNIDGVFAAGDARRGQSLIVWAINEGREVAREVDRYLM 487 Query: 122 GSTVL 126 GS+VL Sbjct: 488 GSSVL 492 >ref|ZP_00098864.1| COG0493: NADPH-dependent glutamate synthase beta chain and related oxidoreductases [Desulfitobacterium hafniense] Length = 505 Score = 146 bits (368), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 72/123 (58%), Positives = 93/123 (75%) Query: 2 TTKFVGDEEGNLKELHTVQIQRMVDETGRKIYQPIPGTEAVYPAQLALIAIGFDGPEQDI 61 T K VG+E+G +KE+HTV++ + D +GR + Q +PG+E V+ A L L+A+GF GPE I Sbjct: 380 TKKIVGNEQGEVKEVHTVEVHWIKDSSGRMVPQEVPGSEKVWEADLVLLAMGFLGPEDAI 439 Query: 62 IEELKLDTDRRSNVKARYGKFNTNVDKVFAAGDMRRGQSLVVWAINEGREAAREVDKYLM 121 EL L+ D RSN+KA Y +F TNV+KVFAAGD RRGQSLVV A+ EG+ AAREVDKYLM Sbjct: 440 PNELNLERDERSNLKAVYEEFETNVEKVFAAGDARRGQSLVVSALQEGKLAAREVDKYLM 499 Query: 122 GST 124 G + Sbjct: 500 GRS 502 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 186,967,264 Number of extensions: 7127655 Number of successful extensions: 19839 Number of sequences better than 1.0e-05: 439 Number of HSP's gapped: 19598 Number of HSP's successfully gapped: 439 Length of query: 127 Length of database: 757,237,578 Length adjustment: 90 Effective length of query: 37 Effective length of database: 512,878,668 Effective search space: 18976510716 Effective search space used: 18976510716 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 120 (50.8 bits)