BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0055.p (204 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAJ65473.1| unknown [environmental sequence] 227 1e-58 ref|NP_461918.1| thymidylate synthetase [Salmonella typhimurium ... 223 2e-57 ref|NP_457395.1| thymidylate synthetase [Salmonella enterica sub... 222 3e-57 ref|NP_355015.1| AGR_C_3709p [Agrobacterium tumefaciens] >gi|252... 221 7e-57 ref|ZP_00092243.1| COG0207: Thymidylate synthase [Azotobacter vi... 221 7e-57 >gb|EAJ65473.1| unknown [environmental sequence] Length = 264 Score = 227 bits (579), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 104/137 (75%), Positives = 119/137 (86%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 +R YLDL+Q ILD G KSDRTGTGT+S+FG Q+RF+LAEGFPLVTTK+ HL+S+IHELL Sbjct: 1 MRAYLDLMQKILDEGTVKSDRTGTGTVSLFGHQMRFNLAEGFPLVTTKKCHLRSIIHELL 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFL GDTNT+YLKEHGVSIWDEWAD NG LGPVYG+QWR+W +ADG IDQI +D IK Sbjct: 61 WFLNGDTNTAYLKEHGVSIWDEWADENGNLGPVYGAQWRSWPAADGSVIDQIQKAVDDIK 120 Query: 128 NNPDSRRHIVSAWNVGE 144 +NPDSRR IVSAWNVGE Sbjct: 121 HNPDSRRIIVSAWNVGE 137 >ref|NP_461918.1| thymidylate synthetase [Salmonella typhimurium LT2] sp|Q8ZMA9|TYSY_SALTY Thymidylate synthase (TS) (TSase) gb|AAL21877.1| thymidylate synthetase [Salmonella typhimurium LT2] Length = 264 Score = 223 bits (567), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 98/137 (71%), Positives = 119/137 (86%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 +++YL+L+Q +LD G K+DRTGTGTLS+FG Q+RF+L EGFPLVTTKR HL+S+IHELL Sbjct: 1 MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQEGFPLVTTKRCHLRSIIHELL 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFL+GDTN +YL E+ V+IWDEWAD NG+LGPVYG QWRAW + DGRHIDQIA V+ +K Sbjct: 61 WFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTPDGRHIDQIATVLSQLK 120 Query: 128 NNPDSRRHIVSAWNVGE 144 N+PDSRR IVSAWNVGE Sbjct: 121 NDPDSRRIIVSAWNVGE 137 >ref|NP_457395.1| thymidylate synthetase [Salmonella enterica subsp. enterica serovar Typhi] ref|NP_806604.1| thymidylate synthetase [Salmonella enterica subsp. enterica serovar Typhi Ty2] sp|Q8Z412|TYSY_SALTI Thymidylate synthase (TS) (TSase) pir||AB0866 thymidylate synthetase [imported] - Salmonella enterica subsp. enterica serovar Typhi (strain CT18) emb|CAD02826.1| thymidylate synthetase [Salmonella enterica subsp. enterica serovar Typhi] gb|AAO70464.1| thymidylate synthetase [Salmonella enterica subsp. enterica serovar Typhi Ty2] Length = 264 Score = 222 bits (566), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 98/137 (71%), Positives = 119/137 (86%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 +++YL+L+Q +LD G K+DRTGTGTLS+FG Q+RF+L EGFPLVTTKR HL+S+IHELL Sbjct: 1 MKQYLELIQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLLEGFPLVTTKRCHLRSIIHELL 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFL+GDTN +YL E+ V+IWDEWAD NG+LGPVYG QWRAW + DGRHIDQIA V+ +K Sbjct: 61 WFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTPDGRHIDQIATVLSQLK 120 Query: 128 NNPDSRRHIVSAWNVGE 144 N+PDSRR IVSAWNVGE Sbjct: 121 NDPDSRRIIVSAWNVGE 137 >ref|NP_355015.1| AGR_C_3709p [Agrobacterium tumefaciens] pir||G97605 hypothetical protein AGR_C_3709 [imported] - Agrobacterium tumefaciens (strain C58, Cereon) gb|AAK87800.1| AGR_C_3709p [Agrobacterium tumefaciens str. C58 (Cereon)] Length = 279 Score = 221 bits (564), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 101/158 (63%), Positives = 130/158 (82%), Gaps = 6/158 (3%) Query: 2 ERGAGQLRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKS 61 E A ++++YLDLL+ +++ G+ + DRTGTGT SVFG Q+RFDL+EGFP++TTK++HL+S Sbjct: 10 ESAAERMKQYLDLLRHVMETGSDRGDRTGTGTRSVFGYQMRFDLSEGFPVLTTKKLHLRS 69 Query: 62 VIHELLWFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAA 121 +IHELLWFL GDTN +YLKE+GVSIWDEWAD NG+LGPVYG+QWR+W + DGRHIDQIA Sbjct: 70 IIHELLWFLNGDTNIAYLKENGVSIWDEWADENGDLGPVYGAQWRSWPAPDGRHIDQIAL 129 Query: 122 VIDSIKNNPDSRRHIVSAWNVGEXXXXRADEAAALPFC 159 +I+++K NP+SRRHIVSAWN DE ALP C Sbjct: 130 LIEALKTNPNSRRHIVSAWNPA-----LVDE-MALPPC 161 >ref|ZP_00092243.1| COG0207: Thymidylate synthase [Azotobacter vinelandii] Length = 264 Score = 221 bits (563), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 106/165 (64%), Positives = 127/165 (76%), Gaps = 1/165 (0%) Query: 8 LRKYLDLLQDILDHGASKSDRTGTGTLSVFGRQLRFDLAEGFPLVTTKRIHLKSVIHELL 67 +++YLDL++ + +HG KSDRTGTGT SVFG Q+RFDLAEGFPLVTTK+ HLKS++HELL Sbjct: 1 MKQYLDLMRLVREHGTFKSDRTGTGTFSVFGHQMRFDLAEGFPLVTTKKCHLKSIVHELL 60 Query: 68 WFLKGDTNTSYLKEHGVSIWDEWADPNGELGPVYGSQWRAWESADGRHIDQIAAVIDSIK 127 WFLKGDTN YLKE+GVSIWDEWAD NG+LGPVYG QWR+W + DGRHIDQIA V++ I+ Sbjct: 61 WFLKGDTNIRYLKENGVSIWDEWADGNGDLGPVYGYQWRSWPAPDGRHIDQIANVVNMIR 120 Query: 128 NNPDSRRHIVSAWNVGEXXXXRADEAAALPFCISVLRGRRQAFLH 172 +NPDSRR IVSAWN + AL F V GR L+ Sbjct: 121 DNPDSRRLIVSAWNPALIDEMKLPPCHAL-FQFYVADGRLSCQLY 164 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.137 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 328,804,040 Number of extensions: 13227358 Number of successful extensions: 27911 Number of sequences better than 1.0e-05: 526 Number of HSP's gapped: 27319 Number of HSP's successfully gapped: 568 Length of query: 204 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 84 Effective length of database: 431,425,698 Effective search space: 36239758632 Effective search space used: 36239758632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)