BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0053.p (470 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P11959|DLD1_BACST Dihydrolipoyl dehydrogenase (E3 component o... 588 e-167 pdb|1EBD|A Chain A, Dihydrolipoamide Dehydrogenase Complexed Wit... 570 e-161 ref|NP_980312.1| pyruvate dehydrogenase complex E3 component, di... 559 e-158 ref|NP_658007.1| pyr_redox, Pyridine nucleotide-disulphide oxido... 559 e-158 ref|NP_833689.1| Dihydrolipoamide dehydrogenase [Bacillus cereus... 559 e-158 >sp|P11959|DLD1_BACST Dihydrolipoyl dehydrogenase (E3 component of pyruvate complex) (Dihydrolipoamide dehydrogenase) pir||S13839 dihydrolipoamide dehydrogenase (EC 1.8.1.4) [validated] - Bacillus stearothermophilus emb|CAA37631.1| dihydrolipoamide dehydrogenase [Geobacillus stearothermophilus] Length = 470 Score = 588 bits (1517), Expect = e-167, Method: Compositional matrix adjust. Identities = 292/469 (62%), Positives = 356/469 (75%), Gaps = 1/469 (0%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 MVVGD +IE +TLV+GAGPGGYVAAIRAAQLGQKV IV+K LGGVCLN GCIPSKALIS Sbjct: 1 MVVGDFAIETETLVVGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALIS 60 Query: 61 AAHQYEAAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAM 120 A+H+YE A+H E G+ AENV +D+AK QE+K VVK++T GV L+KGNK+E+ GEA Sbjct: 61 ASHRYEQAKHSEEMGIKAENVTIDFAKVQEWKASVVKKLTGGVEGLLKGNKVEIVKGEAY 120 Query: 121 FISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMI 180 F+ AN R+ N + Y F N IIATGSRPIEL F F RIL ST AL L E+PKS++ Sbjct: 121 FVDANTVRVVNGDSAQTYTFKNAIIATGSRPIELPNFKFSNRILDSTGALNLGEVPKSLV 180 Query: 181 VIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTGIEIVTNAKA 240 VIGGGYIG ELG Y+ FGTKVTI+EG +L GF+K M ++ K + K G+E+VTNA A Sbjct: 181 VIGGGYIGIELGTAYANFGTKVTILEGAGEILSGFEKQMAAIIKKRLKKKGVEVVTNALA 240 Query: 241 ESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKVD 300 + A + + VTV Y GE+K + ADY+LVTVGRRPNT RGLI+VD Sbjct: 241 KGAEEREDGVTVTYEANGETKTIDADYVLVTVGRRPNT-DELGLEQIGIKMTNRGLIEVD 299 Query: 301 HQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPECS 360 Q RT++PNI+AIGDIVPG ALAHKASYEGK+AAEAI+GH S VDY IPAVVF+DPEC+ Sbjct: 300 QQCRTSVPNIFAIGDIVPGPALAHKASYEGKVAAEAIAGHPSAVDYVAIPAVVFSDPECA 359 Query: 361 SVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIEA 420 SVG E++AKD+G V + KFPFA NGRA++LN +GF+K+V E+ +++GAQI+G A Sbjct: 360 SVGYFEQQAKDEGIDVIAAKFPFAANGRALALNDTDGFLKLVVRKEDGVIIGAQIIGPNA 419 Query: 421 SNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHVV 469 S++IAELGLAIE G T EDIALTIHAHPTLGEI MEAAE+ G PIH++ Sbjct: 420 SDMIAELGLAIEAGMTAEDIALTIHAHPTLGEIAMEAAEVALGTPIHII 468 >pdb|1EBD|A Chain A, Dihydrolipoamide Dehydrogenase Complexed With The Binding Domain Of The Dihydrolipoamide Acetylase pdb|1EBD|B Chain B, Dihydrolipoamide Dehydrogenase Complexed With The Binding Domain Of The Dihydrolipoamide Acetylase Length = 455 Score = 570 bits (1469), Expect = e-161, Method: Compositional matrix adjust. Identities = 283/454 (62%), Positives = 345/454 (75%), Gaps = 1/454 (0%) Query: 7 SIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALISAAHQYE 66 +IE +TLV+GAGPGGYVAAIRAAQLGQKV IV+K LGGVCLN GCIPSKALISA+H+YE Sbjct: 1 AIETETLVVGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALISASHRYE 60 Query: 67 AAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAMFISANE 126 A+H E G+ AENV +D+AK QE+K VVK++T GV L+KGNK+E+ GEA F+ AN Sbjct: 61 QAKHSEEMGIKAENVTIDFAKVQEWKASVVKKLTGGVEGLLKGNKVEIVKGEAYFVDANT 120 Query: 127 ARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMIVIGGGY 186 R+ N + Y F N IIATGSRPIEL F F RIL ST AL L E+PKS++VIGGGY Sbjct: 121 VRVVNGDSAQTYTFKNAIIATGSRPIELPNFKFSNRILDSTGALNLGEVPKSLVVIGGGY 180 Query: 187 IGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTGIEIVTNAKAESAVQN 246 IG ELG Y+ FGTKVTI+EG +L GF+K M ++ K + K G+E+VTNA A+ A + Sbjct: 181 IGIELGTAYANFGTKVTILEGAGEILSGFEKQMAAIIKKRLKKKGVEVVTNALAKGAEER 240 Query: 247 DKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKVDHQGRTN 306 + VTV Y GE+K + ADY+LVTVGRRPNT RGLI+VD Q RT+ Sbjct: 241 EDGVTVTYEANGETKTIDADYVLVTVGRRPNT-DELGLEQIGIKMTNRGLIEVDQQCRTS 299 Query: 307 IPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPECSSVGLTE 366 +PNI+AIGDIVPG ALAHKASYEGK+AAEAI+GH S VDY IPAVVF+DPEC+SVG E Sbjct: 300 VPNIFAIGDIVPGPALAHKASYEGKVAAEAIAGHPSAVDYVAIPAVVFSDPECASVGYFE 359 Query: 367 KEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIEASNLIAE 426 ++AKD+G V + KFPFA NGRA++LN +GF+K+V E+ +++GAQI+G AS++IAE Sbjct: 360 QQAKDEGIDVIAAKFPFAANGRALALNDTDGFLKLVVRKEDGVIIGAQIIGPNASDMIAE 419 Query: 427 LGLAIEMGATLEDIALTIHAHPTLGEIVMEAAEL 460 LGLAIE G T EDIALTIHAHPTLGEI MEAAE+ Sbjct: 420 LGLAIEAGMTAEDIALTIHAHPTLGEIAMEAAEV 453 >ref|NP_980312.1| pyruvate dehydrogenase complex E3 component, dihydrolipoamide dehydrogenase [Bacillus cereus ATCC 10987] gb|AAS42920.1| pyruvate dehydrogenase complex E3 component, dihydrolipoamide dehydrogenase [Bacillus cereus ATCC 10987] Length = 470 Score = 559 bits (1440), Expect = e-158, Method: Compositional matrix adjust. Identities = 278/471 (59%), Positives = 350/471 (74%), Gaps = 2/471 (0%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 MVVGD IE+DT+V+GAGPGGYVAAIRAAQLGQKV I++K+ LGGVCLN GCIPSKALI+ Sbjct: 1 MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAIIEKANLGGVCLNVGCIPSKALIN 60 Query: 61 AAHQYEAAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAM 120 A H+YE A H + G+TAENVKVD+ K QE+KNGVVK++T GV L+KGNK+E+ GEA Sbjct: 61 AGHRYENAMHSDDMGITAENVKVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAY 120 Query: 121 FISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMI 180 F+ AN R+ E + Y F N ++ATGS PIE+ F + R+++ST AL LPEIPK ++ Sbjct: 121 FVDANTLRVMTEDAAQTYTFKNAVLATGSTPIEIPGFKYSKRVINSTGALSLPEIPKKLV 180 Query: 181 VIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTG-IEIVTNAK 239 VIGGGYIG ELG Y+ FGT+VT++E D +L GF+K M+ +V + + K G + I T A Sbjct: 181 VIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVTIHTKAM 240 Query: 240 AESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKV 299 A+ + + V V + V GE + V ADY+LVTVGRRPNT RG+I++ Sbjct: 241 AKGVEETETGVKVSFEVKGEIQTVEADYVLVTVGRRPNT-QEIGLEQVGVKMTDRGIIEI 299 Query: 300 DHQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPEC 359 D Q RTN+PNIYAIGDIVPG LAHKASYEGK+A EAISGH S +DY IPAV FTDPE Sbjct: 300 DEQCRTNVPNIYAIGDIVPGPPLAHKASYEGKVAVEAISGHASAIDYIGIPAVCFTDPEL 359 Query: 360 SSVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIE 419 +SVG T+K+A++ G TV KFPFA NGRA+SLNS +GF+++V E+ L++GAQ+ G Sbjct: 360 ASVGYTKKQAEEAGMTVTVSKFPFAANGRALSLNSTDGFLQLVTRKEDGLLVGAQVAGAG 419 Query: 420 ASNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHVVK 470 AS++I+E+GLAIE G T EDIA TIHAHPTLGEI MEAAE+ G PIH+VK Sbjct: 420 ASDIISEIGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK 470 >ref|NP_658007.1| pyr_redox, Pyridine nucleotide-disulphide oxidoreductase [Bacillus anthracis A2012] ref|NP_846418.1| pyruvate dehydrogenase complex E3 component, dihydrolipoamide dehydrogenase [Bacillus anthracis str. Ames] gb|AAP27904.1| pyruvate dehydrogenase complex E3 component, dihydrolipoamide dehydrogenase [Bacillus anthracis str. Ames] Length = 470 Score = 559 bits (1440), Expect = e-158, Method: Compositional matrix adjust. Identities = 278/471 (59%), Positives = 350/471 (74%), Gaps = 2/471 (0%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 MVVGD IE+DT+V+GAGPGGYVAAIRAAQLGQKV I++K+ LGGVCLN GCIPSKALI+ Sbjct: 1 MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAIIEKANLGGVCLNVGCIPSKALIN 60 Query: 61 AAHQYEAAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAM 120 A H+YE A H + G+TAENVKVD+ K QE+KNGVVK++T GV L+KGNK+E+ GEA Sbjct: 61 AGHRYENAMHSDDMGITAENVKVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAY 120 Query: 121 FISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMI 180 F+ AN R+ E + Y F N ++ATGS PIE+ F + R+++ST AL LPEIPK ++ Sbjct: 121 FVDANTLRVMTEEAAQTYTFKNAVLATGSTPIEIPGFKYSKRVINSTGALSLPEIPKKLV 180 Query: 181 VIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTG-IEIVTNAK 239 VIGGGYIG ELG Y+ FGT+VT++E D +L GF+K M+ +V + + K G + I T A Sbjct: 181 VIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVNIHTKAM 240 Query: 240 AESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKV 299 A+ + + V V + V GE + V ADY+LVTVGRRPNT RG+I++ Sbjct: 241 AKGVEETETGVKVSFEVKGEIQTVEADYVLVTVGRRPNT-QEIGLEQVGVKMTDRGIIEI 299 Query: 300 DHQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPEC 359 D Q RTN+PNIYAIGDIVPG LAHKASYEGK+A EAISGH S +DY IPAV FTDPE Sbjct: 300 DEQCRTNVPNIYAIGDIVPGPPLAHKASYEGKVAVEAISGHASAIDYIGIPAVCFTDPEL 359 Query: 360 SSVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIE 419 +SVG T+K+A++ G TV KFPFA NGRA+SLNS +GF+++V E+ L++GAQ+ G Sbjct: 360 ASVGYTKKQAEEAGMTVTVSKFPFAANGRALSLNSTDGFLQLVTRKEDGLLVGAQVAGAG 419 Query: 420 ASNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHVVK 470 AS++I+E+GLAIE G T EDIA TIHAHPTLGEI MEAAE+ G PIH+VK Sbjct: 420 ASDIISEIGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK 470 >ref|NP_833689.1| Dihydrolipoamide dehydrogenase [Bacillus cereus ATCC 14579] gb|AAP10890.1| Dihydrolipoamide dehydrogenase [Bacillus cereus ATCC 14579] Length = 470 Score = 559 bits (1440), Expect = e-158, Method: Compositional matrix adjust. Identities = 278/471 (59%), Positives = 350/471 (74%), Gaps = 2/471 (0%) Query: 1 MVVGDASIEIDTLVIGAGPGGYVAAIRAAQLGQKVLIVDKSELGGVCLNRGCIPSKALIS 60 MVVGD IE+DT+V+GAGPGGYVAAIRAAQLGQKV I++K+ LGGVCLN GCIPSKALI+ Sbjct: 1 MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAIIEKANLGGVCLNVGCIPSKALIN 60 Query: 61 AAHQYEAAQHGEVFGVTAENVKVDWAKTQEFKNGVVKRMTTGVTSLMKGNKIEVFSGEAM 120 A H+YE A H + G+TAENVKVD+ K QE+KNGVVK++T GV L+KGNK+E+ GEA Sbjct: 61 AGHRYENAMHSDDMGITAENVKVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAY 120 Query: 121 FISANEARLFNEHESPRYKFNNCIIATGSRPIELKPFPFGGRILSSTEALELPEIPKSMI 180 F+ AN R+ E + Y F N ++ATGS PIE+ F + R+++ST AL LPEIPK ++ Sbjct: 121 FVDANTLRVMTEDAAQTYTFKNAVLATGSTPIEIPGFKYSKRVINSTGALSLPEIPKKLV 180 Query: 181 VIGGGYIGAELGQMYSKFGTKVTIIEGLDTVLPGFDKDMTRLVAKNMAKTG-IEIVTNAK 239 VIGGGYIG ELG Y+ FGT+VT++E D +L GF+K M+ +V + + K G + I T A Sbjct: 181 VIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVNIHTKAM 240 Query: 240 AESAVQNDKEVTVKYSVGGESKEVTADYLLVTVGRRPNTXXXXXXXXXXXXXXXRGLIKV 299 A+ + + V V + V GE + V ADY+LVTVGRRPNT RG+I++ Sbjct: 241 AKGVEETETGVKVSFEVKGEIQTVEADYVLVTVGRRPNT-QEIGLEQVGVKMTDRGIIEI 299 Query: 300 DHQGRTNIPNIYAIGDIVPGLALAHKASYEGKIAAEAISGHKSVVDYKVIPAVVFTDPEC 359 D Q RTN+PNIYAIGDIVPG LAHKASYEGK+A EAISGH S +DY IPAV FTDPE Sbjct: 300 DEQCRTNVPNIYAIGDIVPGPPLAHKASYEGKVAVEAISGHASAIDYIGIPAVCFTDPEL 359 Query: 360 SSVGLTEKEAKDKGYTVKSGKFPFAGNGRAVSLNSPEGFIKIVANSENNLVLGAQIVGIE 419 +SVG T+K+A++ G TV KFPFA NGRA+SLNS +GF+++V E+ L++GAQ+ G Sbjct: 360 ASVGYTKKQAEEAGMTVTVSKFPFAANGRALSLNSTDGFLQLVTRKEDGLLVGAQVAGAG 419 Query: 420 ASNLIAELGLAIEMGATLEDIALTIHAHPTLGEIVMEAAELVEGHPIHVVK 470 AS++I+E+GLAIE G T EDIA TIHAHPTLGEI MEAAE+ G PIH+VK Sbjct: 420 ASDIISEIGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK 470 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 650,307,900 Number of extensions: 26330327 Number of successful extensions: 101470 Number of sequences better than 1.0e-05: 2383 Number of HSP's gapped: 94787 Number of HSP's successfully gapped: 2407 Length of query: 470 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 341 Effective length of database: 406,989,807 Effective search space: 138783524187 Effective search space used: 138783524187 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)