BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0049.p (273 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_389342.1| pyruvate dehydrogenase (E1 beta subunit) [Bacil... 371 e-101 sp|P21874|ODPB_BACST Pyruvate dehydrogenase E1 component, beta s... 367 e-100 ref|NP_464578.1| highly similar to pyruvate dehydrogenase (E1 be... 365 e-100 ref|NP_243520.1| pyruvate dehydrogenase E1 (lipoamide) beta subu... 364 e-100 ref|NP_692334.1| pyruvate dehydrogenase E1 beta subunit [Oceanob... 362 4e-99 >ref|NP_389342.1| pyruvate dehydrogenase (E1 beta subunit) [Bacillus subtilis] sp|P21882|ODPB_BACSU Pyruvate dehydrogenase E1 component, beta subunit (S complex, 36 kDa subunit) pir||C36718 pyruvate dehydrogenase (lipoamide) (EC 1.2.4.1) E1 beta chain precursor pdhB - Bacillus subtilis gb|AAA62682.1| pyruvate decarboxylase (E-1) beta subunit emb|CAB13332.1| pyruvate dehydrogenase (E1 beta subunit) [Bacillus subtilis subsp. subtilis str. 168] gb|AAC24933.1| pyruvate decarboxylase E-1 beta subunit [Bacillus subtilis] Length = 325 Score = 371 bits (952), Expect = e-101, Method: Compositional matrix adjust. Identities = 170/251 (67%), Positives = 210/251 (83%) Query: 23 FRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGVKAAELHTDSLE 82 FRP+ EIQF GF++E +D + Q AR+R+RSGGR+ +PV R+PFGGGV ELH DSLE Sbjct: 75 FRPVMEIQFFGFVYEVMDSVSGQMARMRYRSGGRWTSPVTIRSPFGGGVHTPELHADSLE 134 Query: 83 GLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAEVPEGEYTVELG 142 GL+AQ PGIKVVIPS PYDAKGLLI++IRDNDPV F+EH+ LY +FR EVPE EYT+ELG Sbjct: 135 GLVAQQPGIKVVIPSTPYDAKGLLISAIRDNDPVVFLEHMKLYRSFRQEVPEEEYTIELG 194 Query: 143 KANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPIDIDTIVASVKKT 202 KA+V REG+D+SIITYG MVH + KAA++LEKDGI AE++DLRTVSP+DIDTI+ASV+KT Sbjct: 195 KADVKREGTDLSIITYGAMVHESLKAADELEKDGISAEVVDLRTVSPLDIDTIIASVEKT 254 Query: 203 NRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQIEDTWIPTPAR 262 RAIVVQEAQK +G+AA V+A+IN++AIL LEAPVLRVA PDTV+PF+Q E W+P Sbjct: 255 GRAIVVQEAQKQAGIAANVVAEINDRAILSLEAPVLRVAAPDTVFPFSQAESVWLPNHKD 314 Query: 263 VIAAVKKVMEF 273 V+ +KV+EF Sbjct: 315 VLETARKVLEF 325 >sp|P21874|ODPB_BACST Pyruvate dehydrogenase E1 component, beta subunit pir||S14230 pyruvate dehydrogenase (lipoamide) (EC 1.2.4.1) E1-beta chain [validated] - Bacillus stearothermophilus emb|CAA37629.1| pyruvate dehydrogenase (lipoamide) [Geobacillus stearothermophilus] Length = 325 Score = 367 bits (942), Expect = e-100, Method: Compositional matrix adjust. Identities = 168/262 (64%), Positives = 210/262 (80%) Query: 12 GWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGV 71 G + +QGFRP+ EIQF GF++E +D I Q AR+R+R+GGR++ P+ R+PFGGGV Sbjct: 64 GGLAIGLALQGFRPVPEIQFFGFVYEVMDSICGQMARIRYRTGGRYHMPITIRSPFGGGV 123 Query: 72 KAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAE 131 ELH+DSLEGL+AQ PG+KVVIPS PYDAKGLLI++IRDNDPV F+EHL LY +FR E Sbjct: 124 HTPELHSDSLEGLVAQQPGLKVVIPSTPYDAKGLLISAIRDNDPVIFLEHLKLYRSFRQE 183 Query: 132 VPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPID 191 VPEGEYT+ +GKA++ REG D++II YG MVH + KAA +LEK+GI AE++DLRTV P+D Sbjct: 184 VPEGEYTIPIGKADIKREGKDITIIAYGAMVHESLKAAAELEKEGISAEVVDLRTVQPLD 243 Query: 192 IDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQ 251 I+TI+ SV+KT RAIVVQEAQ+ +G+AA V+A+INE+AIL LEAPVLRVA PDTVYPFAQ Sbjct: 244 IETIIGSVEKTGRAIVVQEAQRQAGIAANVVAEINERAILSLEAPVLRVAAPDTVYPFAQ 303 Query: 252 IEDTWIPTPARVIAAVKKVMEF 273 E W+P VI KKVM F Sbjct: 304 AESVWLPNFKDVIETAKKVMNF 325 >ref|NP_464578.1| highly similar to pyruvate dehydrogenase (E1 beta subunit) [Listeria monocytogenes EGD-e] pir||AE1206 pyruvate dehydrogenase (E1 beta chain) homolog PdhB [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC99131.1| PdhB [Listeria monocytogenes] Length = 325 Score = 365 bits (936), Expect = e-100, Method: Compositional matrix adjust. Identities = 167/262 (63%), Positives = 211/262 (80%) Query: 12 GWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGV 71 G + ++GFRP+ EIQF GF+FE +D + Q AR+R+R+GG NAP+ R PFGGGV Sbjct: 64 GGLAIGLALEGFRPVPEIQFFGFVFEVMDSVAGQMARMRYRTGGTRNAPITIRAPFGGGV 123 Query: 72 KAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAE 131 E+H D+LEGL+AQSPG+KVVIPS PYDAKGLLI++IRDNDPV F+EH+ LY +FR E Sbjct: 124 HTPEMHADNLEGLMAQSPGLKVVIPSTPYDAKGLLISAIRDNDPVIFLEHMKLYRSFREE 183 Query: 132 VPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPID 191 VPEGEYTVE+GKA V REG+DVSIITYG MV + KAAE LEKDG+ E+IDLRT+SPID Sbjct: 184 VPEGEYTVEIGKAAVRREGTDVSIITYGAMVQESMKAAEALEKDGVSVEVIDLRTISPID 243 Query: 192 IDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQ 251 ++TI+ASVKKTNRA+VVQEAQK +G+AA ++A+IN+ AIL LEAPV+RVA PD+V+PF+Q Sbjct: 244 VETIIASVKKTNRAVVVQEAQKQAGIAANIVAEINDHAILSLEAPVMRVAAPDSVFPFSQ 303 Query: 252 IEDTWIPTPARVIAAVKKVMEF 273 E W+P +I VK+V+ F Sbjct: 304 AETVWLPNHNDIIERVKEVIAF 325 >ref|NP_243520.1| pyruvate dehydrogenase E1 (lipoamide) beta subunit [Bacillus halodurans] pir||F83981 pyruvate dehydrogenase E1 (lipoamide) beta subunit pdhB [imported] - Bacillus halodurans (strain C-125) dbj|BAB06373.1| pyruvate dehydrogenase E1 (lipoamide) beta subunit [Bacillus halodurans] Length = 325 Score = 364 bits (935), Expect = e-100, Method: Compositional matrix adjust. Identities = 164/251 (65%), Positives = 208/251 (82%) Query: 23 FRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGVKAAELHTDSLE 82 FRP+ E+QF GF+FE D + Q AR+R+RSGG++++P+ R+PFGGGVK ELH D+LE Sbjct: 75 FRPVMEVQFFGFVFEVFDSVAGQMARMRYRSGGKYHSPITVRSPFGGGVKTPELHADNLE 134 Query: 83 GLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAEVPEGEYTVELG 142 GL+AQ+PG+KVVIPS PYDAKGLLI++IRDNDPV ++EH+ LY +FRAEVPE EYT+ LG Sbjct: 135 GLMAQTPGVKVVIPSTPYDAKGLLISAIRDNDPVIYLEHMKLYRSFRAEVPEEEYTIPLG 194 Query: 143 KANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPIDIDTIVASVKKT 202 KA+V REG DVSIITYG MVH++ KAAE+LEK+GI AE+IDLRT+SPIDIDTI+ SVKKT Sbjct: 195 KADVKREGKDVSIITYGAMVHSSLKAAEELEKEGISAEVIDLRTISPIDIDTILESVKKT 254 Query: 203 NRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQIEDTWIPTPAR 262 +R IVVQEAQK +G+ A V ++I E+AILHLEAP++RV+ PDTVYPFA ED W+P Sbjct: 255 SRVIVVQEAQKQAGIGAHVASEIQERAILHLEAPIMRVSAPDTVYPFAAAEDVWLPDFKD 314 Query: 263 VIAAVKKVMEF 273 ++ K V+EF Sbjct: 315 IVEKAKAVIEF 325 >ref|NP_692334.1| pyruvate dehydrogenase E1 beta subunit [Oceanobacillus iheyensis HTE831] dbj|BAC13369.1| pyruvate dehydrogenase E1 (lipoamide) beta subunit [Oceanobacillus iheyensis HTE831] Length = 325 Score = 362 bits (930), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 164/262 (62%), Positives = 213/262 (81%) Query: 12 GWVXXXXGVQGFRPIAEIQFVGFIFEALDQIVVQAARLRWRSGGRFNAPVVFRTPFGGGV 71 G + ++GFRP+ EIQF GF++E +D I Q AR+R+RSGG +NAP+ R PFGGGV Sbjct: 64 GGLAIGLALEGFRPVPEIQFFGFVYEVMDSISGQMARMRYRSGGHYNAPITVRAPFGGGV 123 Query: 72 KAAELHTDSLEGLIAQSPGIKVVIPSNPYDAKGLLIASIRDNDPVFFMEHLNLYHAFRAE 131 ELH DSLEGL+AQ PG+KVVIPS PY+AKGLLI++IRDNDPV F+EH+ LY +FR E Sbjct: 124 HTPELHADSLEGLMAQQPGLKVVIPSTPYEAKGLLISAIRDNDPVVFLEHMKLYRSFRGE 183 Query: 132 VPEGEYTVELGKANVVREGSDVSIITYGLMVHTATKAAEQLEKDGIKAEIIDLRTVSPID 191 VPE +YTVE+GKA+V REGSDV++++YG MVH++ KAAE+LEKDGI+AE+IDLRTVSPID Sbjct: 184 VPEEDYTVEIGKADVKREGSDVTLVSYGAMVHSSLKAAEELEKDGIQAEVIDLRTVSPID 243 Query: 192 IDTIVASVKKTNRAIVVQEAQKSSGVAAEVIAQINEKAILHLEAPVLRVAGPDTVYPFAQ 251 +TI+ASVKKTNR +VVQEAQ+ +GVA +VI++I E+AIL LEAP+LRV+ PDTVY F+ Sbjct: 244 YETILASVKKTNRVVVVQEAQRQAGVAGQVISEIQERAILDLEAPILRVSAPDTVYSFSD 303 Query: 252 IEDTWIPTPARVIAAVKKVMEF 273 E+TW+P ++ V +V+ F Sbjct: 304 AEETWLPNHNDIVEKVNEVINF 325 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 379,889,129 Number of extensions: 14550813 Number of successful extensions: 38305 Number of sequences better than 1.0e-05: 997 Number of HSP's gapped: 37638 Number of HSP's successfully gapped: 1001 Length of query: 273 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 149 Effective length of database: 420,565,302 Effective search space: 62664229998 Effective search space used: 62664229998 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)