BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0044.p (213 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_692433.1| hypothetical protein OB1512 [Oceanobacillus ihe... 171 8e-42 ref|ZP_00061422.1| COG1564: Thiamine pyrophosphokinase [Clostrid... 147 2e-34 ref|NP_833578.1| Thiamin pyrophosphokinase [Bacillus cereus ATCC... 145 8e-34 ref|NP_657827.1| hypothetical protein predicted by GeneMark [Bac... 141 1e-32 ref|NP_980198.1| conserved hypothetical protein [Bacillus cereus... 141 1e-32 >ref|NP_692433.1| hypothetical protein OB1512 [Oceanobacillus iheyensis HTE831] dbj|BAC13468.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 216 Score = 171 bits (434), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 88/191 (46%), Positives = 126/191 (65%), Gaps = 2/191 (1%) Query: 24 EEDFVIGADRGALFLITHGVEPDISVGDFDSVPPEALHQIEAGSKKTITCDPVDKDLTDS 83 E DF IGADRGAL+L + DI++GDFDSV E +I +KK +T +K+ TD Sbjct: 24 EVDFWIGADRGALYLAESNLNIDIALGDFDSVSDEEKQKINKHAKKVLTY-STEKNYTDL 82 Query: 84 EMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTRALQRQVHCSVMDTHNYITLTGS- 142 E+A+ +A Q + ILLFGVTG R+DH L +IQ++ R LQ+ V ++D N I + S Sbjct: 83 ELAIQIASDLQVDRILLFGVTGGRLDHELMNIQLLYRLLQKNVEAKIIDHSNQIEMVESG 142 Query: 143 QTEIHDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATLKLGQSLGVSNILTAERGTVSIE 202 + I++ GYTY+S + T +V G+TLEGF+YPL T++ G SL +SN LT ++GT S Sbjct: 143 KYTINNQGYTYISFISFTNKVEGLTLEGFKYPLIDQTIEWGSSLCISNKLTQKKGTFSFR 202 Query: 203 SGLLLIIQSKD 213 +G+LL+I+S D Sbjct: 203 AGILLLIKSCD 213 >ref|ZP_00061422.1| COG1564: Thiamine pyrophosphokinase [Clostridium thermocellum ATCC 27405] Length = 212 Score = 147 bits (371), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 75/196 (38%), Positives = 122/196 (62%), Gaps = 1/196 (0%) Query: 18 YLSLLDEEDFVIGADRGALFLITHGVEPDISVGDFDSVPPEALHQIEAGSKKTITCDPVD 77 Y DE DF++ AD GAL L G++PD+ +GDFDS+ E L + + + P + Sbjct: 18 YRKFFDEADFIVCADGGALHLQRLGIKPDVLLGDFDSIESEHLEYYMKQNVEILKF-PAE 76 Query: 78 KDLTDSEMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTRALQRQVHCSVMDTHNYI 137 KD+TD+E+A++ A+ ++I++ G TGTR+DHTL++I ++ L R V +++ +N + Sbjct: 77 KDMTDTELAVNTAIDRGYKNIVIIGGTGTRLDHTLSNIFLLKLMLDRGVKGRIINEYNEM 136 Query: 138 TLTGSQTEIHDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATLKLGQSLGVSNILTAERG 197 L TEI Y++LLPLT +V GIT EG YPL G +++G + GVSN ++ Sbjct: 137 FLINDSTEIEAEDGCYLTLLPLTSKVEGITTEGLYYPLRGEAIEMGSTRGVSNCFVEKKA 196 Query: 198 TVSIESGLLLIIQSKD 213 +SI SG+L+ I++++ Sbjct: 197 RISITSGILIAIKTRE 212 >ref|NP_833578.1| Thiamin pyrophosphokinase [Bacillus cereus ATCC 14579] gb|AAP10779.1| Thiamin pyrophosphokinase [Bacillus cereus ATCC 14579] Length = 188 Score = 145 bits (365), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 76/186 (40%), Positives = 115/186 (61%), Gaps = 3/186 (1%) Query: 30 GADRGALFLITHGVEPDISVGDFDSVPPEALHQIEAGSKKTITCDPVDKDLTDSEMALDL 89 DRG L+ G+ P ++ GD+DSV E L + + + + P +KD TD E+A++ Sbjct: 4 AVDRGVYRLLKKGITPAVAFGDYDSVTEEELVWMGQQTNE-LHIVPREKDQTDLEIAINW 62 Query: 90 ALGTQPESILLFGVTGTRIDHTLASIQMMTRALQRQVHCSVMDTHNYITLTGSQTEI--H 147 ALG +P I +FG TG R+DH LA+IQM+ + L+ ++ ++D N IT+ T I Sbjct: 63 ALGQKPALIRIFGATGGRLDHGLANIQMLLKGLEVEIEMCIVDNKNEITVKKVGTHIIEE 122 Query: 148 DLGYTYVSLLPLTPEVTGITLEGFQYPLTGATLKLGQSLGVSNILTAERGTVSIESGLLL 207 + + YVS +P+T V GITL GF+YPLT T++ G +L +SN L E+GT S SG+L+ Sbjct: 123 NKNFPYVSFVPVTEIVEGITLFGFKYPLTNKTIEWGSTLCISNELVEEKGTFSFTSGILM 182 Query: 208 IIQSKD 213 +I+S D Sbjct: 183 LIRSTD 188 >ref|NP_657827.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_846239.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAP27725.1| conserved hypothetical protein [Bacillus anthracis str. Ames] Length = 213 Score = 141 bits (355), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 86/213 (40%), Positives = 125/213 (58%), Gaps = 11/213 (5%) Query: 8 IFAGGDLSAEY---LSLLDEEDFVIGA-DRGALFLITHGVEPDISVGDFDSVPPEALHQI 63 I AGG AEY S + E V A DRG L+ G+ P ++ GD+DSV E L + Sbjct: 5 ILAGG--PAEYCADFSRYENEKVVWAAVDRGVYRLLKGGITPAVAFGDYDSVTEEEL--V 60 Query: 64 EAGSKKT-ITCDPVDKDLTDSEMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTRAL 122 G + + P +KD TD E+A++ AL +P I +FG TG R+DH LA+IQM+ + L Sbjct: 61 WMGQQTNDLHIVPREKDQTDLEIAINWALEQKPALIRIFGATGGRLDHGLANIQMLLKGL 120 Query: 123 QRQVHCSVMDTHNYITLTGSQTEI--HDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATL 180 + + ++D N I++ T I + + YVS +P+T V GITL GF+YPLT T+ Sbjct: 121 EVGIEMCIVDNKNEISVKKVGTHIIEENENFPYVSFVPVTEIVEGITLLGFKYPLTNKTI 180 Query: 181 KLGQSLGVSNILTAERGTVSIESGLLLIIQSKD 213 + G +L +SN L E+GT S SG+L++I+S D Sbjct: 181 EWGSTLCISNELIDEKGTFSFTSGILMVIRSTD 213 >ref|NP_980198.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] gb|AAS42806.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] Length = 213 Score = 141 bits (355), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 84/212 (39%), Positives = 122/212 (57%), Gaps = 9/212 (4%) Query: 8 IFAGGDLSAEY---LSLLDEEDFVIGA-DRGALFLITHGVEPDISVGDFDSVPPEALHQI 63 I AGG AEY S + E V A DRG L+ G+ P ++ GD+DSV E L + Sbjct: 5 ILAGG--PAEYCADFSPYENEKVVWAAVDRGVYRLLKRGIIPAVAFGDYDSVTEEELAWM 62 Query: 64 EAGSKKTITCDPVDKDLTDSEMALDLALGTQPESILLFGVTGTRIDHTLASIQMMTRALQ 123 + P +KD TD E+A++ AL +P I +FG TG R+DH LA+IQM+ + L+ Sbjct: 63 -GQQTNDLHIVPREKDQTDLEIAINWALEQKPALIRIFGATGGRLDHGLANIQMLLKGLE 121 Query: 124 RQVHCSVMDTHNYITLTGSQTEI--HDLGYTYVSLLPLTPEVTGITLEGFQYPLTGATLK 181 + ++D N I++ T I + + YVS +P+T V GITL GF+YPLT ++ Sbjct: 122 VGIEMCIVDNQNEISVKRVGTHIIEENKNFPYVSFVPVTEIVEGITLLGFKYPLTNKKIE 181 Query: 182 LGQSLGVSNILTAERGTVSIESGLLLIIQSKD 213 G +L +SN L E+GT S SG+L++I+S D Sbjct: 182 WGSTLCISNELVEEKGTFSFTSGILMVIRSTD 213 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 301,497,210 Number of extensions: 11972189 Number of successful extensions: 27823 Number of sequences better than 1.0e-05: 74 Number of HSP's gapped: 27767 Number of HSP's successfully gapped: 74 Length of query: 213 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 93 Effective length of database: 431,425,698 Effective search space: 40122589914 Effective search space used: 40122589914 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 123 (52.0 bits)