BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0009.p (177 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAB61133.1| GdmH [Staphylococcus gallinarum] 82 8e-15 ref|NP_623732.1| conserved hypothetical protein [Thermoanaerobac... 76 3e-13 gb|AAB61159.1| putative membrane protein [Staphylococcus epiderm... 75 5e-13 ref|NP_561725.1| hypothetical protein [Clostridium perfringens] ... 74 1e-12 ref|NP_782538.1| membrane associated protein [Clostridium tetani... 74 1e-12 >gb|AAB61133.1| GdmH [Staphylococcus gallinarum] Length = 330 Score = 81.6 bits (200), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 44/85 (51%), Positives = 63/85 (74%), Gaps = 5/85 (5%) Query: 58 RFIVSGGLDRVDYKYTEAEGMKNYLVEQGVPENAIILEDKSTSTYENLRFSQKLMKSKDL 117 +F++SGG + D + +EA+ MKNYL+ QGV EN II E+KS STYENL+FS+KL+K Sbjct: 198 KFLLSGGQGK-DERISEAQAMKNYLISQGVNENKIICENKSQSTYENLQFSKKLIKK--- 253 Query: 118 NTAVII-THTFHGQRALEIARELNY 141 NT ++I ++ FH RA+ IA++L Y Sbjct: 254 NTNILICSNNFHILRAVFIAKKLKY 278 >ref|NP_623732.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM25336.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 192 Score = 76.3 bits (186), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 46/122 (37%), Positives = 71/122 (58%), Gaps = 8/122 (6%) Query: 21 GCGHYSWYGDVGRQAQPRTTXXXLDYGLELYREGKFPRFIVSGGLDRVDYKYTEAEGMKN 80 GC + YGD A L+ L+LY+EG + R+I+ G +EAE K Sbjct: 45 GC---AVYGD----APSPFFKERLEEALKLYKEG-YGRYIIISGGKGPGESISEAEAGKR 96 Query: 81 YLVEQGVPENAIILEDKSTSTYENLRFSQKLMKSKDLNTAVIITHTFHGQRALEIARELN 140 Y +E+GV I+L++KS STYENL FS+++M+ L TA+I+++ FH +RA IA+ + Sbjct: 97 YFLEKGVASKNILLDNKSFSTYENLLFSKEIMEKNSLKTAIIVSNKFHLKRASVIAKRIG 156 Query: 141 YN 142 + Sbjct: 157 ID 158 >gb|AAB61159.1| putative membrane protein [Staphylococcus epidermidis] Length = 330 Score = 75.5 bits (184), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 41/92 (44%), Positives = 65/92 (70%), Gaps = 3/92 (3%) Query: 52 REGKFPRFIVSGGLDRVDYKYTEAEGMKNYLVEQGVPENAIILEDKSTSTYENLRFSQKL 111 ++ K +F++SGG + D + +E++ MK YL+ QGV EN II E+KS STYENL+FS+ L Sbjct: 192 KKDKHTKFLLSGGQGK-DERISESQAMKKYLIAQGVNENRIICENKSQSTYENLKFSKSL 250 Query: 112 MKSKDLNTAVIITHTFHGQRALEIARELNYNR 143 + SK+ T +I ++ +H RA+ IA++L Y+ Sbjct: 251 I-SKN-ATIIICSNNYHILRAVFIAKKLKYSH 280 >ref|NP_561725.1| hypothetical protein [Clostridium perfringens] dbj|BAB80515.1| hypothetical protein [Clostridium perfringens str. 13] Length = 206 Score = 74.3 bits (181), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 45/107 (42%), Positives = 65/107 (60%), Gaps = 5/107 (4%) Query: 74 EAEGMKNYLVEQGVPENAIILEDKSTSTYENLRFSQKLMKSK--DLNTAVIITHTFHGQR 131 E+E MKN+LV +G+ N II EDKS ST++NL FS+K+++ + D + +I+T FH R Sbjct: 96 ESEAMKNFLVNKGINPNRIIEEDKSKSTFQNLEFSKKILEERHADKDEVLIVTSDFHLFR 155 Query: 132 ALEIARELNYNRPELGLTDSQVMSM-LKYKTREILAYTKWKMQQLFL 177 A EIA LN GL +S+ ++Y RE A K +Q+ FL Sbjct: 156 AKEIADYLNIKNE--GLPSKTPISLRVQYMVREFPAMIKLLLQEAFL 200 >ref|NP_782538.1| membrane associated protein [Clostridium tetani E88] gb|AAO36475.1| membrane associated protein [Clostridium tetani E88] Length = 192 Score = 74.3 bits (181), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 39/89 (43%), Positives = 58/89 (65%), Gaps = 1/89 (1%) Query: 44 LDYGLELYREGKFPRFIVSGGLDRVDYKYTEAEGMKNYLVEQGVPENAIILEDKSTSTYE 103 ++ G LY+EG + R+I++ G +EAE MK YL+ +G+ E II EDKSTST E Sbjct: 61 IEEGYRLYKEG-YGRYILASGGKGPGESISEAEAMKRYLISKGIKEEYIIKEDKSTSTME 119 Query: 104 NLRFSQKLMKSKDLNTAVIITHTFHGQRA 132 NL+ K+M+ K L AVI+++ +H +RA Sbjct: 120 NLKNCNKIMEKKGLKNAVIVSNKYHLKRA 148 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 256,103,109 Number of extensions: 9518663 Number of successful extensions: 22432 Number of sequences better than 1.0e-05: 92 Number of HSP's gapped: 22423 Number of HSP's successfully gapped: 92 Length of query: 177 Length of database: 757,237,578 Length adjustment: 117 Effective length of query: 60 Effective length of database: 439,570,995 Effective search space: 26374259700 Effective search space used: 26374259700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 122 (51.6 bits)