BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0475.0007.p (476 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00037358.1| COG1288: Predicted membrane protein [Enteroco... 572 e-162 ref|NP_212977.1| conserved hypothetical integral membrane protei... 327 3e-88 gb|AAB94000.1| unknown [Borrelia afzelii] 322 9e-87 ref|NP_602937.1| Short-chain fatty acids transporter [Fusobacter... 310 5e-83 ref|NP_416801.1| putative S-transferase [Escherichia coli K12] >... 309 8e-83 >ref|ZP_00037358.1| COG1288: Predicted membrane protein [Enterococcus faecium] Length = 466 Score = 572 bits (1474), Expect = e-162, Method: Compositional matrix adjust. Identities = 293/462 (63%), Positives = 355/462 (76%), Gaps = 8/462 (1%) Query: 1 METVKSKWKWSLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQEVVVDGSSRTAIIPGTYHT 60 MET+K K+ + PHSYT LTWI+PSGQF+ V G +T ++PGTYH Sbjct: 1 METMKRKF--TFPHSYTLIFILIILAAILTWIIPSGQFETVVENVAGMEKTTVVPGTYHQ 58 Query: 61 VPKISDQGDLRQGFAQIASAPMEGIINAADVVAFVLIVGGAFGIILQTGAIDRALMALAG 120 + D+ D QGF+ I AP EGII+A +VVAFVLIVGGAFGIIL+TGAIDR L LA Sbjct: 59 I----DKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKTGAIDRGLFTLAK 114 Query: 121 RLKTKGILLIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILILFLGTQ 180 L KGIL+IP++M++FSLGGSTFGMSEE+IPL+A+FI LMF+LG+DS+TAILILFL TQ Sbjct: 115 VLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGYDSMTAILILFLSTQ 174 Query: 181 IGYVGSTVNPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKKNPEKS 240 GYVGST+NPF+VLIAQGVAGV GNP LW R + W V TGIS+ FTM YA RVKKNP S Sbjct: 175 AGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIVFTMLYASRVKKNPLSS 234 Query: 241 AVFQNDILNRQHFIKENDGIEVKFSNRDKLIIGGFIVTLGIIVWGILSKGWYMTEIGALF 300 V+++D+ + F +N E +FS +DKLII GFIV LGI++WGI+SKGWYM EI ALF Sbjct: 235 IVYESDLKVKHKF--QNFDTEKEFSTQDKLIIAGFIVGLGIMIWGIISKGWYMVEISALF 292 Query: 301 FVLGIFSGIVTRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVVAENGMIIDTILNGLA 360 LG+F+G + R SQKEIAE FV+GC +F YAAVIIGLARG+LV+ E GMIIDTILN LA Sbjct: 293 LGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGVLVILEGGMIIDTILNSLA 352 Query: 361 TMLEGLPNYLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDLVGVNREAVVTAYQF 420 +L GLP +LFTTI+L+ +++F VPSSSG AALTMPV+APLGDL+ V+R+ VV + QF Sbjct: 353 NLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVMAPLGDLMNVSRDVVVLSNQF 412 Query: 421 GNGLTNLISPTGGVLLAGLAIARINFGQWLKVILKLFPILWL 462 GNGL N+ISPT GVLLAGLAIA INF +W KV K+F IL L Sbjct: 413 GNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFAILTL 454 >ref|NP_212977.1| conserved hypothetical integral membrane protein [Borrelia burgdorferi] pir||B70205 conserved hypothetical integral membrane protein BB0843 - Lyme disease spirochete gb|AAC67189.1| conserved hypothetical integral membrane protein [Borrelia burgdorferi B31] Length = 482 Score = 327 bits (839), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 179/473 (37%), Positives = 288/473 (60%), Gaps = 22/473 (4%) Query: 1 METVKSKWKWSLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQ-EVVVDGSSRTAIIPGTYH 59 M+ + +P S+T LT+++P+G+FD++ + + DGS R I+ GTY Sbjct: 1 MKLFRRNVMIKMPSSFTIIFSLIVFVTILTYVIPAGKFDKEFKQMGDGSKREIIVAGTYQ 60 Query: 60 TVPKISDQGDLRQGFAQ----IASAPMEGIINAADVVAFVLIVGGAFGIILQTGAIDRAL 115 V D+G +GF I +A +G+ +A +V+ FVLIVGGA+GII++TGAID + Sbjct: 61 YV----DRGS--RGFLHPIMTILTAMSKGMEHAVEVIVFVLIVGGAYGIIMKTGAIDVGI 114 Query: 116 MALAGRLKTKGILLIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILIL 175 L +L K LLIP+ M IFS+GG+ GMSEE +P Y + IPL+ ALG+DS+ I+ Sbjct: 115 YFLIKKLGHKDKLLIPLLMFIFSIGGTVTGMSEETLPFYFVMIPLIVALGYDSLVGAAII 174 Query: 176 FLGTQIGYVGSTVNPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKK 235 LG +G + STVNPF+ IA +A + + R + + V ++ + YA ++KK Sbjct: 175 ALGAGVGTMASTVNPFATGIASAIASISLQDGFYFRIVLYFVSVLAAITYVCVYASKIKK 234 Query: 236 NPEKSAVFQNDILNRQHFIKEN---------DGIEVKFSNRDKLIIGGFIVTLGIIVWGI 286 +P KS V+ + Q+F+K++ + +E F+++ L++ GF++ I+++ I Sbjct: 235 DPSKSLVYSQKDEHYQYFVKKDGLSTGDNAQNALEFTFAHKLVLLLFGFMIL--ILIFSI 292 Query: 287 LSKGWYMTEIGALFFVLGIFSGIVTRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVVA 346 ++ GW+M E+ L+ + I S + ++ + E+ + FVKG + AA++IGLARG+++V Sbjct: 293 VNLGWWMQEMTMLYLGVAIISAFICKLGETEMWDAFVKGSESLLTAALVIGLARGVMIVC 352 Query: 347 ENGMIIDTILNGLATMLEGLPNYLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDL 406 ++G+I DT+LN L LP LF + ++ I F+VPSSSG A+LTMP++APL D Sbjct: 353 DDGLITDTMLNAATNFLYNLPRPLFIILNEIIQIFIGFVVPSSSGHASLTMPIMAPLADF 412 Query: 407 VGVNREAVVTAYQFGNGLTNLISPTGGVLLAGLAIARINFGQWLKVILKLFPI 459 + + R +VV A Q +GL NLI+PT GV++A L I+R+++G W K +L LF I Sbjct: 413 LSIPRASVVIAMQTASGLINLITPTSGVIMAVLGISRLSYGTWFKFVLPLFMI 465 >gb|AAB94000.1| unknown [Borrelia afzelii] Length = 473 Score = 322 bits (826), Expect = 9e-87, Method: Compositional matrix adjust. Identities = 177/462 (38%), Positives = 280/462 (60%), Gaps = 21/462 (4%) Query: 11 SLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQ-EVVVDGSSRTAIIPGTYHTVPKISDQGD 69 +P S+T LT+++P+G+FD++ + DG R I+ GTY T+ D+G Sbjct: 3 KMPSSFTIIFSLIVFVTILTYVIPAGKFDKEFRQIGDGPKREIIVAGTYQTI----DRGP 58 Query: 70 LRQGFAQ----IASAPMEGIINAADVVAFVLIVGGAFGIILQTGAIDRALMALAGRLKTK 125 +GF I +A +G+ +AA+V+ FVLIVGGA+GII++TGAID + +L +L K Sbjct: 59 --RGFLHPIMTILTAMSKGMEHAAEVIIFVLIVGGAYGIIMKTGAIDAGIYSLIKKLGHK 116 Query: 126 GILLIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILILFLGTQIGYVG 185 LLIP+ M IFS+GG+ GMSEE +P Y + IPL+ ALG+D++ + I+ LG +G + Sbjct: 117 DKLLIPLLMFIFSIGGTVTGMSEETLPFYFVMIPLIVALGYDNVVGVAIIALGAGVGTMA 176 Query: 186 STVNPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKKNPEKSAVFQN 245 STVNPF+ IA +A + R + + + +++ + YA R+KK+P KS V+ Sbjct: 177 STVNPFATGIASAIASISLQDGFSFRIVLYFISILVAIIYVCVYASRIKKDPSKSLVYSK 236 Query: 246 DILNRQHFIKE--------NDGIEVKFSNRDKLIIGGFIVTLGIIVWGILSKGWYMTEIG 297 + Q+F+K + +E F+ + L++ GF++ +V+ I+ GW+M E+ Sbjct: 237 KNEHYQYFVKNEISKEDNVQNTLEFTFARKLVLLLFGFMILF--LVFSIVQLGWWMQEMT 294 Query: 298 ALFFVLGIFSGIVTRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVVAENGMIIDTILN 357 L+ + I S + R+ + E+ + FVKG I AA+IIGLARG+++V ++G+I T+LN Sbjct: 295 MLYLGVAIISAFICRLGESEMWDAFVKGSESLITAALIIGLARGVMIVCDDGLITATMLN 354 Query: 358 GLATMLEGLPNYLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDLVGVNREAVVTA 417 L LP F + ++ I F+VPSSSG A+LTMP++APL D + + R +VV A Sbjct: 355 AATNFLYNLPRPFFIILNEIIQIFIGFIVPSSSGHASLTMPIMAPLADFLSIGRSSVVIA 414 Query: 418 YQFGNGLTNLISPTGGVLLAGLAIARINFGQWLKVILKLFPI 459 Q +GL NLI+PT GV++A L I+++++G W K +L LF I Sbjct: 415 MQTSSGLINLITPTSGVIMAVLGISKLSYGTWFKFVLPLFII 456 >ref|NP_602937.1| Short-chain fatty acids transporter [Fusobacterium nucleatum subsp. nucleatum ATCC 25586] gb|AAL94236.1| Short-chain fatty acids transporter [Fusobacterium nucleatum subsp. nucleatum ATCC 25586] Length = 499 Score = 310 bits (794), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 179/464 (38%), Positives = 278/464 (59%), Gaps = 34/464 (7%) Query: 29 LTWIVPSGQFDRQEVVV-----DGSSRTAIIPGTYHTVPKISDQG-DLRQGFAQIASAPM 82 LT+IVP G+F+ QEV +R +PG++ ++ D+G +L++G Sbjct: 23 LTYIVPVGKFEMQEVTYVTNTGAEKTRNVPVPGSFSY--ELDDKGNELKKGIKIFEPGGE 80 Query: 83 EGIIN--------------AADVVAFVLIVGGAFGIILQTGAIDRALMALAGRLKTKGIL 128 G+ N A +VAF+L+VGGAFGIIL+TGA++ + ++ + K ++ Sbjct: 81 VGVTNYVFEGLASGDKWGTAVGIVAFLLVVGGAFGIILKTGAVESGIYSMISKSKGSELV 140 Query: 129 LIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMFALGFDSITAILILFLGTQIGYVGSTV 188 LIP+ ++FSLGG+ FGM EE IP + IP++ +G+DSIT ILI ++ TQIG+ S + Sbjct: 141 LIPVIFILFSLGGAVFGMGEEAIPFAMLIIPIVIDMGYDSITGILITYISTQIGFATSWM 200 Query: 189 NPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGISVGFTMWYAHRVKKNPEKSAVFQNDIL 248 NPFSV +AQGV+G+ R W FT V +T++YA +VK+NPE S ++ D Sbjct: 201 NPFSVAVAQGVSGIPVLSGAGFRIFMWLFFTAFGVIYTIFYARKVKRNPESSIAYKTDGY 260 Query: 249 NRQHFIKENDGIEVKFSNRDKLIIGGFIVTLGIIVWGILSKGWYMTEIGALFFVLGIFSG 308 R +F E G +F KLII I+ + +V+G++ +G+Y+ EI F ++G+ +G Sbjct: 261 FRDNFKSEEQG-NREFKLGHKLIILVLILGMAWVVYGVVKEGYYLPEIATQFVIMGLIAG 319 Query: 309 IV------TRMSQKEIAENFVKGCGEFIYAAVIIGLARGILVV-----AENGMIIDTILN 357 I+ MS +IA +F KG + + AA++IG+A+GI+++ A+ I++TILN Sbjct: 320 IIGVVFKLNNMSVNDIATSFRKGAEDMVGAALVIGMAKGIVLILGGTNADTPTILNTILN 379 Query: 358 GLATMLEGLPNYLFTTIMLVVHNVITFLVPSSSGEAALTMPVLAPLGDLVGVNREAVVTA 417 +A+ L + +M + +V F V S SG+AALTMP++APL DLVGV R+ V A Sbjct: 380 YVASALSNMSAAFCAWVMYIFQSVFNFFVVSGSGQAALTMPIMAPLSDLVGVTRQVAVLA 439 Query: 418 YQFGNGLTNLISPTGGVLLAGLAIARINFGQWLKVILKLFPILW 461 +Q G+G TN+I PT G+L+A L IA+I +G W+K +K IL+ Sbjct: 440 FQLGDGFTNMIVPTSGILMAVLGIAKIEWGVWVKYQIKFQLILF 483 >ref|NP_416801.1| putative S-transferase [Escherichia coli K12] pir||H65001 hypothetical protein b2298 - Escherichia coli (strain K-12) gb|AAC75358.1| putative S-transferase [Escherichia coli K12] Length = 513 Score = 309 bits (792), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 185/491 (37%), Positives = 277/491 (56%), Gaps = 40/491 (8%) Query: 5 KSKWKWSLPHSYTXXXXXXXXXXGLTWIVPSGQFDRQEVV--VDGSSRT--AIIPGT--- 57 K +W++P + TW+VP G FD QEV VDG ++T + P + Sbjct: 15 KPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQEVQYQVDGQTKTRKVVDPHSFRI 74 Query: 58 ---------YHTVPKISDQGDLRQGFAQIASAPMEGIIN------AADVVAFVLIVGGAF 102 YH V ++ GD R G P EG+ + A ++ F+L++GGAF Sbjct: 75 LTNEAGEPEYHRV-QLFTTGDERPGLMNF---PFEGLTSGSKYGTAVGIIMFMLVIGGAF 130 Query: 103 GIILQTGAIDRALMALAGRLKTKGILLIPIAMVIFSLGGSTFGMSEEIIPLYAIFIPLMF 162 GI+++TG ID ++AL + IL IP ++FSLGG+ FGM EE + I PLM Sbjct: 131 GIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSLGGAVFGMGEEAVAFAIIIAPLMV 190 Query: 163 ALGFDSITAILILFLGTQIGYVGSTVNPFSVLIAQGVAGVQGNPQLWLRFIEWTVFTGIS 222 LG+DSIT +L+ ++ TQIG+ S +NPF V++AQG+AGV LR + W + T I Sbjct: 191 RLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQGIAGVPVLSGSGLRIVVWVIATLIG 250 Query: 223 VGFTMWYAHRVKKNPEKSAVFQNDILNRQHFIKENDGIEVKFSNRDKLIIGGFIVTLGII 282 + FTM YA RVKKNP S V ++D R+ K+ D + F+ D L++ + + Sbjct: 251 LIFTMVYASRVKKNPLLSRVHESDRFFRE---KQADVEQRPFTFGDWLVLIVLTAVMVWV 307 Query: 283 VWGILSKGWYMTEIGALFFVLGIFSGIV------TRMSQKEIAENFVKGCGEFIYAAVII 336 +WG++ W++ EI + FF +G+ GI+ M+ +A +F +G I A+++ Sbjct: 308 IWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRLNGMTVNTMASSFTEGARMMIAPALLV 367 Query: 337 GLARGILVVAENG-----MIIDTILNGLATMLEGLPNYLFTTIMLVVHNVITFLVPSSSG 391 G A+GIL++ NG +++TILN +A + GL N + ML+ V F V S SG Sbjct: 368 GFAKGILLLVGNGEAGDASVLNTILNSIANAISGLDNAVAAWFMLLFQAVFNFFVTSGSG 427 Query: 392 EAALTMPVLAPLGDLVGVNREAVVTAYQFGNGLTNLISPTGGVLLAGLAIARINFGQWLK 451 +AALTMP+LAPLGDLVGVNR+ V A+QFG+G +++I PT L+A L + R++F WLK Sbjct: 428 QAALTMPLLAPLGDLVGVNRQVTVLAFQFGDGFSHIIYPTSASLMATLGVCRVDFRNWLK 487 Query: 452 VILKLFPILWL 462 V L +L++ Sbjct: 488 VGATLLGLLFI 498 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.143 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 673,792,452 Number of extensions: 28953232 Number of successful extensions: 115032 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 114869 Number of HSP's successfully gapped: 84 Length of query: 476 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 347 Effective length of database: 406,989,807 Effective search space: 141225463029 Effective search space used: 141225463029 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)