BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0475.0006.p (527 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00037572.1| COG1574: Predicted metal-dependent hydrolase ... 235 2e-60 ref|ZP_00036637.1| hypothetical protein [Enterococcus faecium] 141 4e-32 ref|ZP_00019751.1| hypothetical protein [Chloroflexus aurantiacus] 139 1e-31 ref|NP_923321.1| hypothetical protein glr0375 [Gloeobacter viola... 135 2e-30 gb|EAK45405.1| unknown [environmental sequence] 111 5e-23 >ref|ZP_00037572.1| COG1574: Predicted metal-dependent hydrolase with the TIM-barrel fold [Enterococcus faecium] Length = 451 Score = 235 bits (599), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 121/391 (30%), Positives = 208/391 (53%), Gaps = 6/391 (1%) Query: 133 LDEAFPDRAVIAINRTKSYCWMNTLAQHKYQFAPDECSAESRAHLLKDMMQDRELVRQEF 192 L++ FPD + + C +N+ A+ ++ F C E+ LL + D++ ++ F Sbjct: 52 LNKLFPDIPTVIMASGAETCLLNSAAESRFNFTSQTCYPEAYWRLLHYVFSDKKFIKPLF 111 Query: 193 LDFARMLAKRGVTSIKDIGFDRHSXXXXXXXXXXXXXXXXXRVHFALEPVLQPLDIPAGL 252 + + + L RG+T+IK++GFD RV+F +PV + ++I Sbjct: 112 VSYMKFLNSRGITAIKEMGFDDFYGFTDILKELEESSDLSLRVNFMSQPVSEKMNIDYAK 171 Query: 253 QYKERYQGEFLRFQGYKLMLDGVVADHTGDMLEPYADMPGVTTLRPVDYEAIETAVQAAD 312 + + G+ +RF G+ M DG +++ GD+ +PY + +T + +D+ IE ++ AD Sbjct: 172 KMSALFTGDAIRFSGFNKMTDGSISEMKGDLKQPYTNNSEITNIEKIDWNEIERDLKIAD 231 Query: 313 SHGIKCCLTAEGDAAIRKAVDILEGC-SRRRGDQRIRHSISDLEYPHPDDIRRMGENGIF 371 +HG + L A+GD A+ K V ++E C + G RHSI+DLE DD+++MG+ GI Sbjct: 232 NHGFRFSLHAQGDGAVSKTVSLMEKCQTDINGKLLNRHSITDLELTEKDDLKKMGDLGII 291 Query: 372 AEVYAQILLL--NPSYEEAYMSAVAGKENESRFYNYKSMLEANVPVTIGTDLPLFLTSVP 429 AE+Y QI L NP + + G ++N K M+ N+ ++ GTDLPL +P Sbjct: 292 AEIYPQITSLYDNPLAKVKQIKEYVGPR-AKYYWNRKEMINNNIILSCGTDLPLLYDDIP 350 Query: 430 DSLYAASFRLFSDGSPMGGWQPEQGMPAAEVLKAWTINGARHNGMEERTGTLEAGKAADI 489 +S++ A F+D S + + +E++ AWT GA + G+E++ GTLEAGK ADI Sbjct: 351 ESIFNACGGYFTDDSFQ--FNSNNCLNVSELIDAWTSGGAYNLGLEDKIGTLEAGKLADI 408 Query: 490 AVFDRNLLDTTATEIRDAHVILTITAGQITY 520 ++ +L ++ A V+ TI G+I + Sbjct: 409 VIYSDDLFSIPIKNVKQAKVVTTIYDGKIVF 439 >ref|ZP_00036637.1| hypothetical protein [Enterococcus faecium] Length = 244 Score = 141 bits (355), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 66/203 (32%), Positives = 119/203 (58%), Gaps = 1/203 (0%) Query: 11 RVLLSNAVFTGIEDSVSPAFVALQGERIAAVGNPAEAKDWIGPKTFVHHLKDALIMPGVH 70 ++L S +F G E ++ + I V N D + T + D +MP +H Sbjct: 3 QILRSKMIFDG-EQPPFKGYIVINEGIIEKVENNWNYSDLLNENTELIAYDDQFVMPAIH 61 Query: 71 DNHVFFTGYMSMHRGVDLTHAATIDEALQLLRQDAENRPSEQNVYAYGWSEEGWGQLPGQ 130 DNHVFF+GY++M+ G+DL+ ++ +EA++ ++ ++ +++ +YA+GW W P + Sbjct: 62 DNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKADEPIYAHGWDAALWKTDPSE 121 Query: 131 SLLDEAFPDRAVIAINRTKSYCWMNTLAQHKYQFAPDECSAESRAHLLKDMMQDRELVRQ 190 LL+E + AI++ +S+CWMN +A+ Y F E SAESR L+K+M+ +++L+ + Sbjct: 122 ELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKETSAESRVLLIKEMLANKKLLAE 181 Query: 191 EFLDFARMLAKRGVTSIKDIGFD 213 + +F +L +GV S+K+I FD Sbjct: 182 VYAEFEALLLSKGVISMKEIVFD 204 >ref|ZP_00019751.1| hypothetical protein [Chloroflexus aurantiacus] Length = 533 Score = 139 bits (351), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 128/533 (24%), Positives = 210/533 (39%), Gaps = 28/533 (5%) Query: 12 VLLSNAVFTGIEDSVSPAFVALQGERIAAVGNPAEAKDWIGPKTFVHHLKDALIMPGVHD 71 ++L N F G SV+ A +A++GERI +G +A W P T + L++PG D Sbjct: 3 LILRNVRFPG--QSVAQA-IAIRGERITWIGPDHDAAQWQQPATQIIDGAGMLVLPGFTD 59 Query: 72 NHVFFTGYMSMHRGVDLTHAATIDEALQLLRQDAENRPSEQNVYAYGWSEEGW--GQLPG 129 +H + L A +I + L A P+ + GW + G+LP Sbjct: 60 SHFHLHNGARALSVLRLEQAESIADLQTRLADYAAANPTLPWIIGRGWRYRLFAPGELPT 119 Query: 130 QSLLDEAFPDRAVIAINRTKSYCWMNTLAQHKYQF--APDECSAESRAHLLKDMMQDREL 187 + LLD PDR V+ W NT A D + S+ + D + EL Sbjct: 120 RHLLDAVVPDRPVLLTAFDGHTAWANTAALQIAGILAGADTGNPFSQVVMGNDGLASGEL 179 Query: 188 VRQEFLDFARM--------------------LAKRGVTSIKDIGFDRHSXXXXXXXXXXX 227 +D R LA G+T + ++ D Sbjct: 180 REAPAMDLVRRCIPSPTEAEIRDSVRRALRELAALGLTGVHNMDGDEAQRTRYRDLAAAG 239 Query: 228 XXXXXXRVHFALEPVLQPLDIPAGLQYKERYQGEFLRFQGYKLMLDGVVADHTGDMLEPY 287 R+ ++ P P I +Y+ ++ KL +DGVV T ML PY Sbjct: 240 ELTLRIRLPLSVSPDTDPARITDWAMDARQYRNTLVQTDAVKLFIDGVVEAKTALMLAPY 299 Query: 288 ADMPGVTTLRPVDYEAIETAVQAADSHGIKCCLTAEGDAAIRKAVDILEGCSRRRGDQRI 347 AD G + D + V AD+ G++ C+ A GD +++ +D L + G + Sbjct: 300 ADGSGDCGVANYDADEFIRLVTRADAAGLQVCVHAIGDGGVQQTLDALAQAQQINGQRDA 359 Query: 348 RHSISDLEYPHPDDIRRMGENGIFAEVYAQILLLNPSYEEAYMSAVAGKENESRFYNYKS 407 RH + E P D+ R G+ A V + + + V G++ + ++ Sbjct: 360 RHRVEHAEIVDPTDLPRFAHLGVIASVQPLHVDFGMDRDNPWWRLV-GEQRLRYGFPWRD 418 Query: 408 MLEANVPVTIGTDLPLFLTSVPDSLYAASFRLFSDGSPMGGWQPEQGMPAAEVLKAWTIN 467 +L+A + +G+D P+ + + A R DGS P+Q + AE L +T Sbjct: 419 LLQAGARMALGSDWPVADPNPLRGIQVACTRGKLDGSAADSDFPDQRLTIAEALAGYTTW 478 Query: 468 GARHNGMEERTGTLEAGKAADIAVFDRNLLDTTATEIRDAHVILTITAGQITY 520 A + G L G AD+ + DR++ +I V+LT+ G+I + Sbjct: 479 AAYAGHRDHELGRLAPGYLADLVLLDRDITTIPPDQIATTRVMLTMVGGRIVF 531 >ref|NP_923321.1| hypothetical protein glr0375 [Gloeobacter violaceus] dbj|BAC88316.1| glr0375 [Gloeobacter violaceus PCC 7421] Length = 582 Score = 135 bits (341), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 135/543 (24%), Positives = 215/543 (39%), Gaps = 31/543 (5%) Query: 9 ADRVLLSNAVFTGIEDSVSPAFVALQGERIAAVGNPAEAKDWIGPKTFVHHLKDALIMPG 68 AD VL + V+TG+ +A+ RI G+ G KT + L + PG Sbjct: 25 ADLVLTNGRVWTGVPQQPWVEAMAVAKGRILKTGSREAIGKLTGRKTQIIDLGGRMAAPG 84 Query: 69 VHDNHVFFTGYMSMHRGVDLTHAATIDEALQLLRQDAENRPSEQNVYAYGWSEEGW--GQ 126 +D H+ F G VDL ++ + + + + A P + GW G+ Sbjct: 85 FNDAHIHFLGGALDLAIVDLNGTTSLAQIQRRVAEYARAHPERAWIEGVGWQYSALPGGR 144 Query: 127 LPGQSLLDEAFPDRAVIAINRTKSYCWMNTLAQHKYQFAPDECSAESRAHLLKDMMQDRE 186 LP ++ LD R V + W+N+ A + P + AE +++D + Sbjct: 145 LPTRADLDAIEAKRPVFLRSYDGHTVWVNSRALEIAKITP-QTRAEGFGEIVRDPVSGEA 203 Query: 187 ----------LVRQEFLDFAR------------MLAKRGVTSIKDIGFDRHSXXXXXXXX 224 LVR+ + R + A G+TSI++ G S Sbjct: 204 TGVLKESAVGLVRRHIPEPTREEQLVALRAGMKLAASLGITSIQNAGGSAESFALYEELL 263 Query: 225 XXXXXXXXXRVHFALEPVLQPLDIPAGLQYKERYQGE-FLRFQGYKLMLDGVVADHTGDM 283 V ++ P ++ + ERY+ LR KL++DGV+ HT M Sbjct: 264 RRGELTVRTSVALSVGPQTTDAELTEFARIHERYKNHPMLRAGAAKLLIDGVIETHTAAM 323 Query: 284 LEPYADMPGVTTLRPVDYEAIETAVQAADSHGIKCCLTAEGDAAIRKAVDILEGCSRRRG 343 L PY+D P E A G++ A GD A+R A+D E Sbjct: 324 LTPYSDKPDTRGEPAYSAEVFNQLALRAHKAGLQLYTHAIGDRAVRIALDGYEYVKNIAQ 383 Query: 344 DQRIRHSISDLEYPHPDDIRRMGENGIFAEVYAQILLLNPSYEEAYMSAVAGKENESRFY 403 R I +E P DI R G+ A + + + +P E + AV G E R + Sbjct: 384 PADPRFRIEHIEVVAPQDIPRFARLGVIASM--EPIHADPGTVEVWSRAV-GPERTERAF 440 Query: 404 NYKSMLEANVPVTIGTDLPLFLTSVP-DSLYAASFRLFSDGSPMGGWQPEQGMPAAEVLK 462 + + + +D P ++ P L++A R +G+P GGW Q + L+ Sbjct: 441 AWADLAAGGARLVFSSDWPAAISLDPLRGLHSAVNRRTLEGTPPGGWVARQRVDLQRALR 500 Query: 463 AWTINGARHNGMEERTGTLEAGKAADIAVFDRNLLDTTATEIRDAHVILTITAGQITY-D 521 A+T GA + E G + G AAD+ VF ++L +I V+LT+ G++ Y D Sbjct: 501 AYTAEGAYASFEENFKGRIAPGMAADLVVFSQDLFTIDPLQIGSTKVVLTVFDGKVIYRD 560 Query: 522 AAR 524 A R Sbjct: 561 AER 563 >gb|EAK45405.1| unknown [environmental sequence] Length = 374 Score = 111 bits (277), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 85/292 (29%), Positives = 140/292 (47%), Gaps = 27/292 (9%) Query: 248 IPAGLQYKERYQGEFLRFQGYKLMLDGVVADHTGDMLEPYADMPGVTTLRP-VDYEAIET 306 IP ++ ++ Y E + K+M DG+V ++T +++ Y ++PG T P ++ E ++ Sbjct: 85 IPHLIELRKNYSSELVSPTTVKIMQDGLVENYTAVLVDHYHNVPGPTKGIPMIEPEFLKE 144 Query: 307 AVQAADSHGIKCCLTAEGDAAIRKAVDILEGCSRRRGDQRIRHSISDLEYPHPDDIRRMG 366 V A D+ G + A GD A R+++D +E G RH +S L+ HPDD R Sbjct: 145 VVTALDALGFQLHFHALGDGAARQSLDAVEESVYENGQLGNRHHVSHLQLIHPDDFDRFA 204 Query: 367 ENGIFAEVYAQILLLNPSYEEAYMSAVA----GKENESRFYNYKSMLEANVPVTIGTDL- 421 E EV A L +Y Y++ +A G E Y KS+ A + G+D Sbjct: 205 E----LEVVANFQPL-WAYTGDYLTELAAPFIGAERLRWSYAIKSLQNAGALIAFGSDWS 259 Query: 422 -----PLFLTSVPDSLYAASFRLFSDGSPMGGWQPEQGMPA--------AEVLKAWTING 468 P + + +A+ + +P+ PEQ +P + A+TIN Sbjct: 260 VSSANPFYQIETAITRQSATEITALENAPV---DPEQDVPLNIEESIDLKTAIDAFTINA 316 Query: 469 ARHNGMEERTGTLEAGKAADIAVFDRNLLDTTATEIRDAHVILTITAGQITY 520 A N E TG++E GK AD+AV D+NL + + EI + +V+LT+ G + + Sbjct: 317 AFVNKSENLTGSIEVGKYADLAVLDQNLFEIESAEISETNVLLTLFDGNVVH 368 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 739,982,008 Number of extensions: 30094194 Number of successful extensions: 65388 Number of sequences better than 1.0e-05: 178 Number of HSP's gapped: 65258 Number of HSP's successfully gapped: 187 Length of query: 527 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 397 Effective length of database: 404,274,708 Effective search space: 160497059076 Effective search space used: 160497059076 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)