BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0048.p (412 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243111.1| aminopeptidase [Bacillus halodurans] >gi|252897... 521 e-146 ref|NP_654259.1| Peptidase_M29, Thermophilic metalloprotease (M2... 495 e-139 ref|NP_830199.1| Aminopeptidase [Bacillus cereus ATCC 14579] >gi... 494 e-138 ref|NP_976687.1| aminopeptidase AmpS [Bacillus cereus ATCC 10987... 493 e-138 ref|NP_389328.1| aminopeptidase [Bacillus subtilis] >gi|2507254|... 471 e-131 >ref|NP_243111.1| aminopeptidase [Bacillus halodurans] pir||E83930 aminopeptidase BH2245 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05964.1| aminopeptidase [Bacillus halodurans] Length = 410 Score = 521 bits (1342), Expect = e-146, Method: Compositional matrix adjust. Identities = 251/412 (60%), Positives = 303/412 (73%), Gaps = 2/412 (0%) Query: 1 MPQLQAQLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWX 60 M Q L KYA+LAV++GVN+QPGQTL++ PISA E VRL+ KAY GA V V W Sbjct: 1 MTTFQENLEKYAELAVKVGVNIQPGQTLVIRTPISALELVRLVAKKAYEAGAKNVHVEWS 60 Query: 61 XXXEFITRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIA 120 E +TR F A E F + P W A E AENGAAFL + +DPD LK D ER+A Sbjct: 61 D--EELTRTKFHLAPDEAFHEYPAWIAKGFEELAENGAAFLSITGQDPDLLKDADPERVA 118 Query: 121 NYQKTRGEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVR 180 N K G+AL +R M+DKVSWSIVA PS+ WA K+ PD D V+KLWEAIF R Sbjct: 119 NLNKAGGKALKTFRSYIMADKVSWSIVAAPSEGWAKKISPDESKDRAVEKLWEAIFAATR 178 Query: 181 LDREDPVAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAK 240 D+ DPVAAW+ HL TL +K N LN ++Y +LHY APGTDL+IELP+ HLW G S N Sbjct: 179 TDQADPVAAWKNHLKTLNEKMNELNKRRYHALHYKAPGTDLTIELPDTHLWVSGGSTNKD 238 Query: 241 GHSFVANMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQ 300 G FVAN+PTEEVFTA KTGVNGTV TKPL+YGG +ID F++TF+ G++V AEQG Sbjct: 239 GVDFVANIPTEEVFTAAKKTGVNGTVTNTKPLNYGGTLIDQFTLTFKDGKVVDFTAEQGY 298 Query: 301 EALEYLIGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEG 360 E L+ L+ DEGA+Y+GEVALVPH+SPISE++++++NTLFDENASNHLAIGTAYAF +EG Sbjct: 299 ETLKRLLDTDEGARYIGEVALVPHQSPISETDLIFYNTLFDENASNHLAIGTAYAFNIEG 358 Query: 361 GKEMNKDELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWAF 412 GKEM+++EL G+NTS+THVDFMIGS+EMDI GIT DG REPVF KGNWAF Sbjct: 359 GKEMSEEELEQAGINTSITHVDFMIGSSEMDIDGITKDGKREPVFRKGNWAF 410 >ref|NP_654259.1| Peptidase_M29, Thermophilic metalloprotease (M29) [Bacillus anthracis A2012] ref|NP_842876.1| aminopeptidase AmpS [Bacillus anthracis str. Ames] gb|AAP24362.1| aminopeptidase AmpS [Bacillus anthracis str. Ames] Length = 409 Score = 495 bits (1275), Expect = e-139, Method: Compositional matrix adjust. Identities = 239/409 (58%), Positives = 300/409 (73%), Gaps = 2/409 (0%) Query: 4 LQAQLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWXXXX 63 + L KYA LAV +GVN+QPGQTL +SAP+ A +FVRL+T KAY GA V V+W Sbjct: 3 FEQTLEKYAALAVNVGVNIQPGQTLSISAPLEAVQFVRLVTEKAYKSGAKHVYVDWND-- 60 Query: 64 EFITRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIANYQ 123 E +TR F+ A E F + P+W A E A+ GAAF+ + AE+PD LKG+DS RIA Sbjct: 61 ETLTRLKFDLAPEEAFAEFPSWKANAREELAKEGAAFMSIYAENPDLLKGVDSTRIATAH 120 Query: 124 KTRGEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVRLDR 183 K GEA+ YR+ +DKVSW ++++P++ WA KVFPD +E+ KLW+AIF R D Sbjct: 121 KVAGEAMKVYRDYVQADKVSWCVISVPTKEWAAKVFPDAAPEEQEAKLWDAIFKATRADL 180 Query: 184 EDPVAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAKGHS 243 E+PV AW+EH TL K + LN K YK+LHY PGTDL+IELPE H+WA S+N K Sbjct: 181 ENPVEAWKEHDKTLHTKVDYLNEKHYKALHYTGPGTDLTIELPEKHVWAGAGSLNEKNVP 240 Query: 244 FVANMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQEAL 303 F+AN+PTEEVFT PLKTGVNG V +TKPL++ GNIID F++TFE GRIV AE G+EAL Sbjct: 241 FMANIPTEEVFTMPLKTGVNGQVSSTKPLAFAGNIIDNFTLTFENGRIVDYKAEVGEEAL 300 Query: 304 EYLIGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEGGKE 363 ++L+ D+G+ +LGEVALVPH SPIS +NIL++NTLFDENAS HLAIG+AYAF L GGK Sbjct: 301 KHLVETDKGSHFLGEVALVPHDSPISNTNILFYNTLFDENASCHLAIGSAYAFNLVGGKT 360 Query: 364 MNKDELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWAF 412 M+K+EL +G NTS+TH DFMIGSA++DI GIT DG EP+F KGNWAF Sbjct: 361 MSKEELAENGANTSITHNDFMIGSAKLDIDGITADGRHEPIFRKGNWAF 409 >ref|NP_830199.1| Aminopeptidase [Bacillus cereus ATCC 14579] gb|AAP07400.1| Aminopeptidase [Bacillus cereus ATCC 14579] Length = 409 Score = 494 bits (1271), Expect = e-138, Method: Compositional matrix adjust. Identities = 238/409 (58%), Positives = 299/409 (73%), Gaps = 2/409 (0%) Query: 4 LQAQLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWXXXX 63 + L KYA LAV +GVN+QPGQTL +SAP+ A +FVRL+T KAY GA V V+W Sbjct: 3 FEQTLEKYAALAVNVGVNIQPGQTLSISAPLEAVQFVRLVTEKAYKSGAKHVYVDWND-- 60 Query: 64 EFITRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIANYQ 123 E +TR F+ A E F + P+W A E A+ GAAF+ + AE+PD LKG+++ RIA Sbjct: 61 ETLTRLKFDLAPEEAFAEFPSWKAHAREELAKEGAAFMSIYAENPDLLKGVEASRIATAH 120 Query: 124 KTRGEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVRLDR 183 K GEA+ YR+ +DKVSW ++++P++ WA KVFPDV +E+ KLW+AIF R D Sbjct: 121 KVAGEAMKVYRDYVQADKVSWCVISVPTKEWAAKVFPDVAPEEQEAKLWDAIFKATRADL 180 Query: 184 EDPVAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAKGHS 243 E+PV AW+EH +TL K + LN K YK+LHY PGTDL+IELPE H+WA S+N K Sbjct: 181 ENPVEAWKEHDETLHTKVDYLNEKHYKALHYTGPGTDLTIELPEKHVWAGAGSLNEKNVP 240 Query: 244 FVANMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQEAL 303 F+AN+PTEEVFT PLKTGVNG V +TKPL + GNIID F++TFE GRIV AE G+EAL Sbjct: 241 FMANIPTEEVFTMPLKTGVNGQVSSTKPLVFAGNIIDNFTLTFENGRIVDYKAEAGEEAL 300 Query: 304 EYLIGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEGGKE 363 ++L+ DEG+ +LGEVALVPH SPIS +N+L++NTLFDENAS HLAIG AYAF L GGK Sbjct: 301 KHLVETDEGSHFLGEVALVPHDSPISNTNVLFYNTLFDENASCHLAIGNAYAFNLVGGKT 360 Query: 364 MNKDELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWAF 412 M+K+EL +G N S+TH DFMIGSAE+DI GIT DG EP+F KGNWAF Sbjct: 361 MSKEELAENGANASITHNDFMIGSAELDIDGITADGRHEPIFRKGNWAF 409 >ref|NP_976687.1| aminopeptidase AmpS [Bacillus cereus ATCC 10987] gb|AAS39295.1| aminopeptidase AmpS [Bacillus cereus ATCC 10987] Length = 409 Score = 493 bits (1269), Expect = e-138, Method: Compositional matrix adjust. Identities = 239/409 (58%), Positives = 298/409 (72%), Gaps = 2/409 (0%) Query: 4 LQAQLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWXXXX 63 + L KYA LAV +GVN+QPGQTL +SAP+ A +FVRL+T KAY GA V V+W Sbjct: 3 FEQTLEKYAALAVNVGVNIQPGQTLSISAPLEAVQFVRLVTEKAYKSGAKHVYVDWND-- 60 Query: 64 EFITRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIANYQ 123 E +TR F+ A E F + P+W A E A+ GAAF+ + AE+PD LKG++ RIA Sbjct: 61 ETLTRLKFDLAPEEAFAEFPSWKAHAREELAKEGAAFMSIYAENPDLLKGVEPTRIATAH 120 Query: 124 KTRGEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVRLDR 183 K GEA+ YR+ +DKVSW ++++P++ WA KVFPDV +E+ KLW+AIF R D Sbjct: 121 KVAGEAMKVYRDYVQADKVSWCVISVPTKEWAAKVFPDVAPEEQEAKLWDAIFKATRADL 180 Query: 184 EDPVAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAKGHS 243 E+PV AW+EH TL K + LN K YK+LHY PGTDL+IELPE H+WA S+N K Sbjct: 181 ENPVEAWKEHDKTLHTKVDYLNEKHYKALHYTGPGTDLTIELPEKHVWAGAGSLNEKNVP 240 Query: 244 FVANMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQEAL 303 F+AN+PTEEVFT PLKTGVNG V +TKPL++ GNIID F++TFE GRIV AE G+EAL Sbjct: 241 FMANIPTEEVFTMPLKTGVNGQVSSTKPLAFAGNIIDNFTLTFENGRIVDYKAEVGEEAL 300 Query: 304 EYLIGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEGGKE 363 ++L+ DEG+ +LGEVALVPH SPIS +NIL++NTLFDENAS HLAIG AYAF L GGK Sbjct: 301 KHLVETDEGSHFLGEVALVPHDSPISNTNILFYNTLFDENASCHLAIGNAYAFNLVGGKT 360 Query: 364 MNKDELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWAF 412 M+K+EL +G N S+TH DFMIGSAE+DI GIT DG EP+F KGNWAF Sbjct: 361 MSKEELAKNGANASITHEDFMIGSAELDIDGITADGRHEPIFRKGNWAF 409 >ref|NP_389328.1| aminopeptidase [Bacillus subtilis] sp|P39762|AMPS_BACSU Aminopeptidase ampS pir||C69585 aminopeptidase ampS - Bacillus subtilis emb|CAB13318.1| aminopeptidase [Bacillus subtilis subsp. subtilis str. 168] gb|AAC24920.1| aminopeptidase [Bacillus subtilis] Length = 410 Score = 471 bits (1211), Expect = e-131, Method: Compositional matrix adjust. Identities = 227/412 (55%), Positives = 291/412 (70%), Gaps = 2/412 (0%) Query: 1 MPQLQAQLSKYADLAVRIGVNVQPGQTLIVSAPISAAEFVRLITAKAYAIGASQVKVNWX 60 M +L+ YA LAV +GVNVQ GQ ++V+A +FVRLI AY GA V VNW Sbjct: 1 MDSFSQKLNTYAQLAVEVGVNVQKGQYVVVNASTDVRDFVRLIVKHAYEKGAKNVTVNWQ 60 Query: 61 XXXEFITRQLFEHAAPEVFTKPPTWFAGEITEFAENGAAFLHVIAEDPDALKGIDSERIA 120 + + +E A E F + P W A E A+NGAAF+ V++ +PD LKGIDS+RIA Sbjct: 61 DDE--VAKLKYELAPFEAFEEYPEWEAKGREELAKNGAAFISVVSSNPDLLKGIDSKRIA 118 Query: 121 NYQKTRGEALTKYRELQMSDKVSWSIVAIPSQAWADKVFPDVPADERVDKLWEAIFHTVR 180 +QK G+AL YR+ SDKVSW++V S WA KVFP +E + LWE IF R Sbjct: 119 AFQKAAGKALHTYRQYIQSDKVSWTVVGAASAGWAHKVFPGKSEEEAIHLLWEEIFKATR 178 Query: 181 LDREDPVAAWQEHLDTLEQKANVLNAKKYKSLHYIAPGTDLSIELPEGHLWAQGDSINAK 240 ++ ++PV AW H L +K + LN + Y +LHY A GTDL+I+LP H+WA S+N Sbjct: 179 VNEDNPVQAWINHDQNLHEKVDHLNERHYAALHYQAEGTDLTIKLPRKHVWAGAGSVNES 238 Query: 241 GHSFVANMPTEEVFTAPLKTGVNGTVKATKPLSYGGNIIDGFSITFEQGRIVSVAAEQGQ 300 GH F+ANMPTEEVFT P K GV+G V +TKPLSYGGNII+ F++TFE GRIV + AE+G+ Sbjct: 239 GHEFMANMPTEEVFTLPQKDGVDGVVSSTKPLSYGGNIIENFTLTFENGRIVDIKAEKGE 298 Query: 301 EALEYLIGLDEGAKYLGEVALVPHKSPISESNILYFNTLFDENASNHLAIGTAYAFCLEG 360 + L+ L+ DEG+ YLGEVALVP+ SPIS+SNIL++NTLFDENASNHLAIG+AYAF +EG Sbjct: 299 DILKELVETDEGSHYLGEVALVPYDSPISQSNILFYNTLFDENASNHLAIGSAYAFNIEG 358 Query: 361 GKEMNKDELIAHGLNTSVTHVDFMIGSAEMDIYGITVDGTREPVFLKGNWAF 412 GK+M+++EL+ GLN S+THVDFMIGS +M+I GIT DG REP+F GNWAF Sbjct: 359 GKQMSREELVKEGLNESITHVDFMIGSKDMNIDGITADGKREPIFRNGNWAF 410 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 613,874,623 Number of extensions: 25411629 Number of successful extensions: 64310 Number of sequences better than 1.0e-05: 102 Number of HSP's gapped: 64217 Number of HSP's successfully gapped: 102 Length of query: 412 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 284 Effective length of database: 409,704,906 Effective search space: 116356193304 Effective search space used: 116356193304 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)