BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0472.0012.p (534 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P43263|NPRE_BACBR Bacillolysin precursor (Neutral protease) >... 495 e-138 sp|P29148|NPRE_PAEPO Bacillolysin precursor (Neutral protease) >... 476 e-133 dbj|BAA06144.1| hydrolase [Lactobacillus sp.] >gi|1094070|prf||2... 409 e-113 pir||I40227 neutral proteinase (EC 3.4.24.-) - Bacillus megateri... 404 e-111 sp|P05806|NPRE_BACCE Bacillolysin precursor (Neutral protease) >... 400 e-110 >sp|P43263|NPRE_BACBR Bacillolysin precursor (Neutral protease) pir||PN0114 microbial metalloproteinase (EC 3.4.24.-) precursor - Bacillus brevis emb|CAA43589.1| microbial metalloproteinases [Brevibacillus brevis] prf||1802380A metalloprotease Length = 527 Score = 495 bits (1274), Expect = e-138, Method: Compositional matrix adjust. Identities = 270/534 (50%), Positives = 338/534 (63%), Gaps = 9/534 (1%) Query: 1 MKKS-LASLLSGIVALGSFASVGIAAESTERSQPAELFTGSTPISITGQSLKTPAGDGSE 59 MKKS LA+ L+ +A+G + A + + + + TP I + P G + Sbjct: 1 MKKSYLATSLTLSIAVGVSGFTSVPAFAKTKIDYHKQW--DTPQYIG--EVWEPEGAKGD 56 Query: 60 EQVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTL 119 + V+ YLE K + N+ F+I + N +T ++HYRL + NGIP+YG QT+ Sbjct: 57 DVVWSYLEKYKDEFRIQG--NVEDHFEIVNEARNKETDTKHYRLQEVYNGIPIYGFQQTV 114 Query: 120 HIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAP 179 HID GNVTS LG + D+ + L + +++ +A+ A+ D + G + P Sbjct: 115 HIDADGNVTSFLGQFIPDLDSN--KQLKKKPKLNEQKAVKQAIKDVEGEVGEKPDFIQDP 172 Query: 180 EADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDLTGTGTGVN 239 EA LY ++ E LAY E+N L+PEP R YFI A G V+ KYN+L+ +T TG GV Sbjct: 173 EAKLYIYVHEDESYLAYAVELNFLDPEPGRWMYFIDAHSGDVINKYNMLDHVTATGKGVL 232 Query: 240 GDTKTFETRLSGSTYQLYDNTRGKGIVTYTAKNRXXXXXXXXXXXXNVWSDRAGVDAHTY 299 GDTK FET GSTY L D TRGKGI TYTA NR N W+D A VDAH + Sbjct: 233 GDTKQFETTKQGSTYMLKDTTRGKGIETYTANNRTSLPGTLMTDSDNYWTDGAAVDAHAH 292 Query: 300 AERTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSYNNAFWNGSQIVFGDGDGTTFTLLSGD 359 A++TYDY+ RNS D G IRS+VHY T YNNAFWNGSQ+V+GDGDGTTF LSG Sbjct: 293 AQKTYDYFRNVHNRNSYDGNGAVIRSTVHYSTRYNNAFWNGSQMVYGDGDGTTFLPLSGG 352 Query: 360 LDVVGHELTHGVTENTANLEYYGEPGALNESFSDIIGNSIEGENWLVGDKVYTPGIPGDA 419 LDVV HELTH VTE TA L Y E GALNES SDI G ++ ++WL+G+ +YTPG GDA Sbjct: 353 LDVVAHELTHAVTERTAGLVYQNESGALNESMSDIFGAMVDNDDWLMGEDIYTPGRSGDA 412 Query: 420 LRSLANPTLYGQPDKYSDRYTGSSDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIGRE 479 LRSL +P YG PD YS RYTGS DNGGVHTNSGINNKA YL+A+GGT GV V GIGR Sbjct: 413 LRSLQDPAAYGDPDHYSKRYTGSQDNGGVHTNSGINNKAAYLLAEGGTHYGVRVNGIGRT 472 Query: 480 DAVQIYYNALVYYLTTSSDFSAARAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 D +IYY+AL +YLT S+FSA R A + SAT+LFGA S QV AV AY+AVGV Sbjct: 473 DTAKIYYHALTHYLTPYSNFSAMRRAAVLSATDLFGANSRQVQAVNAAYDAVGV 526 >sp|P29148|NPRE_PAEPO Bacillolysin precursor (Neutral protease) pir||A41335 microbial metalloproteinase (EC 3.4.24.-) precursor, extracellular - Bacillus polymyxa dbj|BAA00734.1| extracellular neutral protease [Paenibacillus polymyxa] Length = 590 Score = 476 bits (1225), Expect = e-133, Method: Compositional matrix adjust. Identities = 293/600 (48%), Positives = 374/600 (62%), Gaps = 78/600 (13%) Query: 1 MKKSLASLLSGIVALGSFASVGIAAESTERSQPAELFTGSTPISITGQSLKTPAGDGSEE 60 MKK SLL G + LGS AS G +AES+ S PA+L TP T Q K P+ ++ Sbjct: 1 MKKVWFSLLGGAMLLGSVAS-GASAESS-VSGPAQL----TPTFHTEQ-WKAPSSVSGDD 53 Query: 61 QVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTLH 120 V+ YL K +L ++R +F+I ++ T+ K+G HYRL QY+NGIPVYGA+QT+H Sbjct: 54 IVWSYLNRQKKSLLGVDSSSVREQFRIVDR-TSDKSGVSHYRLKQYVNGIPVYGAEQTIH 112 Query: 121 IDKSGNVTSLLGTVV-EDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYG--PLGEAQI 177 + KSG VTS LG V+ ED + Q IS +EA+ A +A + P + I Sbjct: 113 VGKSGEVTSYLGAVINEDQQEEATQGTT--PKISASEAVYTAYKEAAARIEALPTSDDTI 170 Query: 178 APEA--------DLYYFIVGGEPKLAY-KTEVNV-------------------------- 202 + +A D Y + L+ K E+++ Sbjct: 171 SKDAEEPSSVSKDTYAEAANNDKTLSVDKDELSLDKASVLKDSKIEAVEAEKSSIAKIAN 230 Query: 203 LEPE---PLRIRYFISAEDGTVLF--KYNILE---------------------DLT--GT 234 L+PE + Y+ +D +++ + N+LE D+ T Sbjct: 231 LQPEVDPKAELYYYPKGDDLLLVYVTEVNVLEPAPLRTRYIIDANDGSIVFQYDIINEAT 290 Query: 235 GTGVNGDTKTFETRLSGSTYQLYDNTRGKGIVTYTAKNRXXXXXXXXXXXXNVWSDRAGV 294 G GV GD+K+F T SGS+YQL D TRG GIVTYTA NR NVW+D AGV Sbjct: 291 GKGVLGDSKSFTTTASGSSYQLKDTTRGNGIVTYTASNRQSIPGTLLTDADNVWNDPAGV 350 Query: 295 DAHTYAERTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSYNNAFWNGSQIVF--GDGDGTT 352 DAH YA +TYDYY FGRNS+D +GLQ+RS+VHY + YNNAFWNGSQ+ + GDGDG+T Sbjct: 351 DAHAYAAKTYDYYKSKFGRNSIDGRGLQLRSTVHYGSRYNNAFWNGSQMTYGDGDGDGST 410 Query: 353 FTLLSGDLDVVGHELTHGVTENTANLEYYGEPGALNESFSDIIGNSIEGENWLVGDKVYT 412 F SGD DVVGHELTHGVTE T+NLEYYGE GALNE+FSD+IGN I+ +NWLVGD +YT Sbjct: 411 FIAFSGDPDVVGHELTHGVTEYTSNLEYYGESGALNEAFSDVIGNDIQRKNWLVGDDIYT 470 Query: 413 PGIPGDALRSLANPTLYGQPDKYSDRYTGSSDNGGVHTNSGINNKAFYLVAQGGTFNGVT 472 P I GDALRS++NPTLY QP YS+ Y GSSDNGGVHTNSGI NKA+YL+AQGGTF+GVT Sbjct: 471 PNICGDALRSMSNPTLYDQPHHYSNLYKGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVT 530 Query: 473 VAGIGREDAVQIYYNALVYYLTTSSDFSAARAAVIQSATELFGAGSAQVTAVTQAYNAVG 532 V GIGR+ AVQIYY+A YLT+SSDFS ARAAVIQ+A +L+GA SA+ TA ++++AVG Sbjct: 531 VNGIGRDAAVQIYYSAFTNYLTSSSDFSNARAAVIQAAKDLYGANSAEATAAAKSFDAVG 590 >dbj|BAA06144.1| hydrolase [Lactobacillus sp.] prf||2105285A neutral protease Length = 566 Score = 409 bits (1052), Expect = e-113, Method: Compositional matrix adjust. Identities = 247/571 (43%), Positives = 331/571 (57%), Gaps = 47/571 (8%) Query: 2 KKSLASLLSGIVALGSFASVGIAAESTERSQPAELF--TGSTPISITGQSLKTPAGDGSE 59 KKSLA +L+ +A+ +F G A ++ + + T +P ++G L G +E Sbjct: 3 KKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQSPEFVSGD-LTEATGKKAE 61 Query: 60 EQVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTL 119 V+ YL + KG+ L K + + F++K+ + ++ T S RL Q G+PV+G+ Q Sbjct: 62 SVVFDYLNAAKGDYKLGEK-SAQDSFKVKQAKKDAVTDSTVLRLQQVYEGVPVWGSTQVA 120 Query: 120 HIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAP 179 H+ K G++ L GTV D+ + L I +AI A D G + ++ P Sbjct: 121 HVSKDGSLKVLSGTVAPDL--DKKEKLKNKNKIEGAKAIEIAQKD----LGVTPKYEVEP 174 Query: 180 EADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDL-------- 231 +ADLY + G E AY +N LEP P YFI A+ G VL KYN L+ + Sbjct: 175 KADLYVYQNGEETTYAYVVNLNFLEPSPGNYYYFIEADSGKVLNKYNKLDHVANEDKSPV 234 Query: 232 ---------------------TGTGTGVNGDTKTFETRLSGSTYQLYDNTRGKGIVTYTA 270 GTG GV GDTK+ T LS S+Y L DNTRG I TY A Sbjct: 235 KQEAPKQEAKPAVKPVTGTNAVGTGKGVLGDTKSLNTTLSASSYYLQDNTRGATIFTYDA 294 Query: 271 KNRXXXXXXXXXXXXNVWSDRAGVDA---HTYAERTYDYYLQHFGRNSLDNKGLQIRSSV 327 KNR NV++ A H YA +TYDYY F RNS+++ G ++S+V Sbjct: 295 KNRSTLPGTLWVDADNVFNAAYDAAAVDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTV 354 Query: 328 HYYTSYNNAFWNGSQIVFGDGDGTTFTLLSGDLDVVGHELTHGVTENTANLEYYGEPGAL 387 HY + YNNAFWNGSQ+V+GDGDG TFT LSG +DV+GHELTH VTE +++L Y E GAL Sbjct: 355 HYGSKYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTEYSSDLIYQNESGAL 414 Query: 388 NESFSDIIGNSIE-----GENWLVGDKVYTPGIPGDALRSLANPTLYGQPDKYSDRYTGS 442 NE+ SD+ G +E +W +G+ +YTPG GDALRS+++PT YG PD YS RYTG+ Sbjct: 415 NEAISDVFGTLVEYYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGT 474 Query: 443 SDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIGREDAVQIYYNALVYYLTTSSDFSAA 502 SDNGGVHTNSGI NKA YL+A GGT GVTV GIG++ IYY A Y T S+ FS A Sbjct: 475 SDNGGVHTNSGIINKAAYLLANGGTHYGVTVNGIGKDKVGAIYYRANTQYFTQSTTFSQA 534 Query: 503 RAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 RA ++Q+A +L+GA SA+V AV Q+Y+AVGV Sbjct: 535 RAGLVQAAADLYGASSAEVAAVKQSYSAVGV 565 >pir||I40227 neutral proteinase (EC 3.4.24.-) - Bacillus megaterium emb|CAA52964.1| neutral protease [Bacillus megaterium] prf||2109196C neutral protease Length = 562 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 243/565 (43%), Positives = 326/565 (57%), Gaps = 41/565 (7%) Query: 2 KKSLASLLSGIVALGSFASVGIAAESTERSQPAELFTG--STPISITGQSLKTPAGDGSE 59 K++L LLS + SFA ++ + E + +P I+G+ L P+ ++ Sbjct: 5 KQALKVLLSVGILSSSFAFAHTSSAAPNNVLSTEKYNKEIKSPEFISGK-LSGPSSQKAQ 63 Query: 60 EQVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTL 119 + V+ Y+ + K L + +N + F++ E + + RL Q N IPV+G+ Q Sbjct: 64 DVVFHYMNTNKDKYKLGN-ENAQNSFKVTEVVKDPVEQATVVRLQQVYNNIPVWGSTQLA 122 Query: 120 HIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAP 179 H+ K G + + GTV D+ + + L K + +AI AA D + P E +P Sbjct: 123 HVAKDGTLKVVSGTVAPDLDKK--EKLKGQKQVDSKKAIQAAEKDL--GFKPTYEK--SP 176 Query: 180 EADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDLTG------ 233 ++LY + G + AY +N L PEP YF+ A G VL KYN ++ + G Sbjct: 177 SSELYVYQNGSDTTYAYVVNLNFLSPEPGNYYYFVDAISGKVLDKYNTIDSVAGPKADVK 236 Query: 234 -----------------TGTGVNGDTKTFETRLSGSTYQLYDNTRGKGIVTYTAKNRXXX 276 +G GV GDTK+ +T LS STY L DNTRG I TY AKNR Sbjct: 237 QAAKPAAKPVTGTNAIGSGKGVLGDTKSLKTTLSSSTYYLQDNTRGATIYTYDAKNRTSL 296 Query: 277 XXXXXXXXXNVWS---DRAGVDAHTYAERTYDYYLQHFGRNSLDNKGLQIRSSVHYYTSY 333 N ++ D A VDAH YA TYDYY F RNS DN G ++S+VHY + Y Sbjct: 297 PGTLWTDTDNTYNATRDAAAVDAHYYAGVTYDYYKNKFNRNSYDNAGAPLKSTVHYSSGY 356 Query: 334 NNAFWNGSQIVFGDGDGTTFTLLSGDLDVVGHELTHGVTENTANLEYYGEPGALNESFSD 393 NNAFWNGSQ+V+GDGDGTTF LSG LDV+GHELTH VTE ++NL Y E GALNE+ SD Sbjct: 357 NNAFWNGSQMVYGDGDGTTFVPLSGGLDVIGHELTHAVTERSSNLIYQYESGALNEAISD 416 Query: 394 IIGNSIE-----GENWLVGDKVYTPGIPGDALRSLANPTLYGQPDKYSDRYTGSSDNGGV 448 I G +E +W +G+ +YTPG GDALRS++NP YG PD YS RYTGSSDNGGV Sbjct: 417 IFGTLVEYYDNRNPDWEIGEDIYTPGTSGDALRSMSNPAKYGDPDHYSKRYTGSSDNGGV 476 Query: 449 HTNSGINNKAFYLVAQGGTFNGVTVAGIGREDAVQIYYNALVYYLTTSSDFSAARAAVIQ 508 HTNSGI NKA YL+A GGT GVTV GIG + +IYY A Y T S+ FS ARA ++Q Sbjct: 477 HTNSGIINKAAYLLANGGTHYGVTVTGIGGDKLGKIYYRANTLYFTQSTTFSQARAGLVQ 536 Query: 509 SATELFGAGSAQVTAVTQAYNAVGV 533 +A +L+G+GS +V +V ++++AVGV Sbjct: 537 AAADLYGSGSQEVISVGKSFDAVGV 561 >sp|P05806|NPRE_BACCE Bacillolysin precursor (Neutral protease) pir||HYBSU bacillolysin (EC 3.4.24.28) precursor [validated] - Bacillus cereus gb|AAA22620.1| neutral protease Length = 566 Score = 400 bits (1028), Expect = e-110, Method: Compositional matrix adjust. Identities = 248/571 (43%), Positives = 333/571 (58%), Gaps = 47/571 (8%) Query: 2 KKSLASLLSGIVALGSFASVGIAAESTERSQPAELF--TGSTPISITGQSLKTPAGDGSE 59 KKSLA +L+ +A+ +F G A ++ + + T +P I+G L G +E Sbjct: 3 KKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQSPEFISGD-LTEATGKKAE 61 Query: 60 EQVYKYLESVKGNLHLSSKDNIRGKFQIKEQQTNSKTGSQHYRLSQYINGIPVYGADQTL 119 V+ YL + KG+ L K + + F++K+ + ++ T S R+ Q G+PV+G+ Q Sbjct: 62 SVVFDYLNAAKGDYKLGEK-SAQDSFKVKQVKKDAVTDSTVVRMQQVYEGVPVWGSTQVA 120 Query: 120 HIDKSGNVTSLLGTVVEDVYQTIPQPLVQLKTISDTEAISAAVTDATQQYGPLGEAQIAP 179 H+ K G++ L GTV D+ + L I +AI A Q G + ++ P Sbjct: 121 HVSKDGSLKVLSGTVAPDL--DKKEKLKNKNKIEGAKAIEIA----QQDLGVTPKYEVEP 174 Query: 180 EADLYYFIVGGEPKLAYKTEVNVLEPEPLRIRYFISAEDGTVLFKYNILEDLT------- 232 +ADLY + G E AY +N L+P P YFI A+ G VL K+N ++ +T Sbjct: 175 KADLYVYQNGEETTYAYVVNLNFLDPSPGNYYYFIEADSGKVLNKFNTIDHVTNDDKSPV 234 Query: 233 ----------------------GTGTGVNGDTKTFETRLSGSTYQLYDNTRGKGIVTYTA 270 GTG GV GDTK+ T LSGS+Y L DNTRG I TY A Sbjct: 235 KQEAPKQDAKAVVKPVTGTNKVGTGKGVLGDTKSLNTTLSGSSYYLQDNTRGATIFTYDA 294 Query: 271 KNRXXXXXXXXXXXXNVWSDRAGVDA---HTYAERTYDYYLQHFGRNSLDNKGLQIRSSV 327 KNR NV++ A H YA +TYDYY F RNS+++ G ++S+V Sbjct: 295 KNRSTLPGTLWADADNVFNAAYDAAAVDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTV 354 Query: 328 HYYTSYNNAFWNGSQIVFGDGDGTTFTLLSGDLDVVGHELTHGVTENTANLEYYGEPGAL 387 HY ++YNNAFWNGSQ+V+GDGDG TFT LSG +DV+GHELTH VTEN++NL Y E GAL Sbjct: 355 HYGSNYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTENSSNLIYQNESGAL 414 Query: 388 NESFSDIIGNSIE-----GENWLVGDKVYTPGIPGDALRSLANPTLYGQPDKYSDRYTGS 442 NE+ SDI G +E +W +G+ +YTPG GDALRS+++PT YG PD YS RYTGS Sbjct: 415 NEAISDIFGTLVEFYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGS 474 Query: 443 SDNGGVHTNSGINNKAFYLVAQGGTFNGVTVAGIGREDAVQIYYNALVYYLTTSSDFSAA 502 SDNGGVHTNSGI NK YL+A GGT GVTV GIG++ IYY A Y T S+ FS A Sbjct: 475 SDNGGVHTNSGIINKQAYLLANGGTHYGVTVTGIGKDKLGAIYYRANTQYFTQSTTFSQA 534 Query: 503 RAAVIQSATELFGAGSAQVTAVTQAYNAVGV 533 RA +Q+A +L+GA SA+V AV Q+++AVGV Sbjct: 535 RAGAVQAAADLYGANSAEVAAVKQSFSAVGV 565 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 814,452,860 Number of extensions: 36774916 Number of successful extensions: 66638 Number of sequences better than 1.0e-05: 171 Number of HSP's gapped: 66068 Number of HSP's successfully gapped: 172 Length of query: 534 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 404 Effective length of database: 404,274,708 Effective search space: 163326982032 Effective search space used: 163326982032 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 128 (53.9 bits)