BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0013.p (344 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00097261.1| COG0642: Signal transduction histidine kinase... 237 3e-61 ref|NP_782405.1| sensor protein resE [Clostridium tetani E88] >g... 167 4e-40 ref|NP_782041.1| sensor protein resE [Clostridium tetani E88] >g... 157 4e-37 ref|NP_347091.1| Sensory transduction histidine kinase (HAMP, Hi... 153 4e-36 ref|ZP_00060007.1| COG0642: Signal transduction histidine kinase... 152 9e-36 >ref|ZP_00097261.1| COG0642: Signal transduction histidine kinase [Desulfitobacterium hafniense] Length = 331 Score = 237 bits (605), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 126/285 (44%), Positives = 184/285 (64%), Gaps = 3/285 (1%) Query: 58 GVRNWTLRRQITSFMDSVDEMVDRAIHGREKMTHFDETSLSSLEHKLWRYIEINKTNEQN 117 GV W RR+ MDS++ M+DRAI G T FDE+ LS+ E K+ R++ + + + Q+ Sbjct: 34 GVTFWQ-RRKTRRIMDSLNTMLDRAIEGSFAETAFDESLLSATEAKMARFLGVCQVSSQS 92 Query: 118 LETEKNTIKELISDISHQTKTPLSSIMMYSQLLAETPELSGNTRLLLNEIESQSEKLEWL 177 L EK+ IK LI+DISHQTKTP++++++YSQLL E L + + +Q++KL++L Sbjct: 93 LAAEKDKIKGLIADISHQTKTPIANLLLYSQLLEEHELPEEGA-LCVKALSAQAQKLDFL 151 Query: 178 ITSLVKLSRLEAGMITLQSTSGPVIETITRAVSHIYTRAEHKGIDLQIECDPLTRARHDH 237 I +LVK SRLEAG+I + G V I A++ I +AE KGI ++ E RA +D Sbjct: 152 IGALVKASRLEAGIIAVTPRQGEVQALIEAALAQIRLKAEEKGIRIEAE-RAAGRAYYDP 210 Query: 238 KWTSEALFNLLENAVKYTKAGGTIRISAESNEMFTRIDVSDTGIGIQRDELEHIFKRFYR 297 KWT+EAL+N+L+N VKY+ IRI A E+F RID+SD G+GI E IF RFYR Sbjct: 211 KWTAEALYNILDNGVKYSPPQSLIRIRAIPYELFYRIDISDEGMGIAEAEQSQIFARFYR 270 Query: 298 GCSARDYEGIGIGLYLTQKIISIQGGFITAASEAGKGTKLTVFLP 342 + EG+G+GL+L ++I++ GG+I +S+ G+G +VFLP Sbjct: 271 SPAVSGQEGVGLGLFLAREILAAGGGYIKVSSKPGQGAVFSVFLP 315 >ref|NP_782405.1| sensor protein resE [Clostridium tetani E88] gb|AAO36342.1| sensor protein resE [Clostridium tetani E88] Length = 414 Score = 167 bits (422), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 86/229 (37%), Positives = 136/229 (59%), Gaps = 1/229 (0%) Query: 112 KTNEQNLETEKNTIKELISDISHQTKTPLSSIMMYSQLLAETPELSGNTRLLLNEIESQS 171 K N ++E EK + L+SDISHQ KTP+SS+++Y+ +L R L Q Sbjct: 181 KNNFLSIEREKEFLINLLSDISHQLKTPISSLIIYNDILLNRTIDEDKRREFLENSSKQL 240 Query: 172 EKLEWLITSLVKLSRLEAGMITLQSTSGPVIETITRAVSHIYTRAEHKGIDLQIEC-DPL 230 ++EWLI SL+KL++L+AG I + + + +T+ A+ + +A+++ +DL+ + Sbjct: 241 NRIEWLIKSLLKLAKLDAGAIKFEKINSDINKTVKEAIEALRAKAKYEKVDLRFHSKNSN 300 Query: 231 TRARHDHKWTSEALFNLLENAVKYTKAGGTIRISAESNEMFTRIDVSDTGIGIQRDELEH 290 HD W SEA+ NL +N++++TK GG + I E +FTRI V D G GI ++E+ H Sbjct: 301 IIMEHDSNWVSEAIINLTKNSLEHTKEGGLVEIITEKTSIFTRIIVKDNGEGIYKEEIPH 360 Query: 291 IFKRFYRGCSARDYEGIGIGLYLTQKIISIQGGFITAASEAGKGTKLTV 339 IF RF++G ++ E +GIGL L++ II G I AS+ G GT T+ Sbjct: 361 IFDRFFKGRRNKNVESVGIGLALSKSIIEAHDGMIEVASKVGFGTTFTI 409 >ref|NP_782041.1| sensor protein resE [Clostridium tetani E88] gb|AAO35978.1| sensor protein resE [Clostridium tetani E88] Length = 417 Score = 157 bits (397), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 92/249 (36%), Positives = 146/249 (58%), Gaps = 4/249 (1%) Query: 94 ETSLSSLEHKLWRYIEINKTNEQNLETEKNTIKELISDISHQTKTPLSSIMMYSQLLAET 153 E + L H I K + L+ +K +K +ISDISHQ KTPL+S++ ++LL Sbjct: 163 EGDFAILAHNFNLMSNILKLSLHKLKEDKIFLKNIISDISHQLKTPLTSLLTINELLLTR 222 Query: 154 PELSGNTRL-LLNEIESQSEKLEWLITSLVKLSRLEAGMITLQSTSGPVIETITRAVSHI 212 E+ + R+ L + SQ ++EWLI SL+K++RLEA I + + I I A+S + Sbjct: 223 KEMKDDVRIDFLQKSGSQLNRMEWLIISLLKIARLEAQAINFEKRNTLGINFIQDALSSL 282 Query: 213 YTR--AEHKGIDLQIECDPLTRARHDHKWTSEALFNLLENAVKYTKAGGTIRISAESNEM 270 + A+H+ I + D + D +WT EA+ N+++N +++TK GG I++ + Sbjct: 283 KFKSDAKHQKIMVSGNLDNVYFYV-DEQWTIEAILNIIKNCIEHTKEGGEIKVHISETPL 341 Query: 271 FTRIDVSDTGIGIQRDELEHIFKRFYRGCSARDYEGIGIGLYLTQKIISIQGGFITAASE 330 F+RI + D G GI + +L HIF+RFY+G ++ + E +GIGL LT+ II QGG I S+ Sbjct: 342 FSRILIEDNGEGIDKKDLPHIFERFYKGSNSVNAESVGIGLALTKVIIERQGGTILVNSK 401 Query: 331 AGKGTKLTV 339 G G+K T+ Sbjct: 402 KGIGSKFTI 410 >ref|NP_347091.1| Sensory transduction histidine kinase (HAMP, HisKA, HATPase domains) [Clostridium acetobutylicum] pir||D96955 sensory transduction histidine kinase (HAMP, HisKA, HATPase domains) [imported] - Clostridium acetobutylicum gb|AAK78431.1| Sensory transduction histidine kinase (HAMP, HisKA, HATPase domains) [Clostridium acetobutylicum] Length = 416 Score = 153 bits (387), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 91/274 (33%), Positives = 147/274 (53%), Gaps = 4/274 (1%) Query: 72 MDSVDEMVDRAIHGRE--KMTHFDETSLSSLEHKLWRYIEINKTNEQNLETEKNTIKELI 129 ++ V E + I+G ++ + E L L R I + NL+ EK +K ++ Sbjct: 138 INEVHEGAQKIINGNYSLRLNDYREGELGKLSASFNRMSSIIENTLNNLKEEKIFLKNMM 197 Query: 130 SDISHQTKTPLSSIMMYSQLLAETPELSGNTR-LLLNEIESQSEKLEWLITSLVKLSRLE 188 DISHQ KTPLSS+ ++++LL +T L+E + Q +K+EWLI +L+K++RL+ Sbjct: 198 QDISHQLKTPLSSLKIFNELLLNQNIKDEDTMDEFLHESKKQLDKMEWLIINLLKMARLD 257 Query: 189 AGMITLQSTSGPVIETITRAVSHIYTRAEHKGIDLQIECDPLTRARHDHKWTSEALFNLL 248 AG I ++ + ET+ ++S + K + +E +HD KW EA N++ Sbjct: 258 AGAIDFKNEKRSIEETVCESISLLKPMYTLKNQKVSVESTDKAYFKHDKKWLLEAFSNII 317 Query: 249 ENAVKYTKAGGTIRISAESNEMFTRIDVSDTGIGIQRDELEHIFKRFYRGCSARDYEGIG 308 +N +++T G I+I + + ++ + D G GI +D+L HIF+RFY+G + G Sbjct: 318 KNCIEHTGDNGEIKIHVYRSPIMVKVTIEDNGSGISKDDLPHIFERFYKGKGVYNKSSTG 377 Query: 309 IGLYLTQKIISIQGGFITAASEAGKGTKLT-VFL 341 IGL + + II QGGFIT S GT VFL Sbjct: 378 IGLSMAKSIIENQGGFITVKSAVSAGTIFNIVFL 411 >ref|ZP_00060007.1| COG0642: Signal transduction histidine kinase [Clostridium thermocellum ATCC 27405] Length = 357 Score = 152 bits (385), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 100/300 (33%), Positives = 168/300 (56%), Gaps = 15/300 (5%) Query: 48 LFVSCMLLLGGVRNWTLR----RQITSFMDSVDEMVDRAIHGREKMTH--FDETSLSSLE 101 FV C+ + + ++ L R+I F VD R +HG E + + E L+ LE Sbjct: 58 FFVLCLCFVFTLVHFALTYLRYRKIAEFTREVD----RVLHGAESINFGSYSEGELAILE 113 Query: 102 HKLWRYIEINKTNEQNLETEKNTIKELISDISHQTKTPLSSI-MMYSQLLAETPELSGNT 160 ++ + + + L+ +K + + I+DISHQ +TPL+SI ++ SQLL P+LS Sbjct: 114 SEISKLTIRLREQAEALKKDKTFLADSIADISHQIRTPLTSINILVSQLL--KPKLSDEH 171 Query: 161 RL-LLNEIESQSEKLEWLITSLVKLSRLEAGMITLQSTSGPVIETITRAVSHIYTRAEHK 219 R+ + E+E +++WLIT+L+K+S+++A ++ Q + V E + RA+ I E K Sbjct: 172 RIEIAKELELMLMRIDWLITALLKMSKIDAEVVRFQRKTVSVAELVRRAIEPIAIPMELK 231 Query: 220 GIDLQIECDPLTRARHDHKWTSEALFNLLENAVKYTKAGGTIRISAESNEMFTRIDVSDT 279 + + + D W+ EA+ N+L+N +++T GG I I A N +FT I+++D Sbjct: 232 EQQIDVFMEGNETFIGDLSWSVEAIGNILKNCMEHTPKGGRIVIKACENAIFTEIEITDN 291 Query: 280 GIGIQRDELEHIFKRFYRGCSARDYEGIGIGLYLTQKIISIQGGFITAASEAGKGTKLTV 339 G GI R++L H+F+RFY+G ++ ++ +GIGL L + II Q G I A + G K TV Sbjct: 292 GPGIDREDLPHLFERFYKGKNSGEH-SMGIGLALARMIIVSQNGTIKAQNNHDGGAKFTV 350 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 453,757,331 Number of extensions: 17241272 Number of successful extensions: 71137 Number of sequences better than 1.0e-05: 4491 Number of HSP's gapped: 63848 Number of HSP's successfully gapped: 4500 Length of query: 344 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 218 Effective length of database: 415,135,104 Effective search space: 90499452672 Effective search space used: 90499452672 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)