BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0011.p (842 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_782407.1| ABC transporter ATP-binding protein [Clostridiu... 103 1e-20 ref|ZP_00100370.1| COG0577: ABC-type antimicrobial peptide trans... 97 2e-18 ref|NP_782039.1| putative permease [Clostridium tetani E88] >gi|... 89 4e-16 ref|NP_783744.1| ABC transporter-associated permease [Clostridiu... 82 8e-14 ref|NP_347094.1| Predicted ATP transporter permease component [C... 81 9e-14 >ref|NP_782407.1| ABC transporter ATP-binding protein [Clostridium tetani E88] gb|AAO36344.1| ABC transporter ATP-binding protein [Clostridium tetani E88] Length = 845 Score = 103 bits (258), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 194/846 (22%), Positives = 337/846 (39%), Gaps = 152/846 (17%) Query: 78 ETLKKHSSIREYGVSLNAGD--LRNEVFKDNRVEVKQIDESYARHSFVNFIEGGLPAAEN 135 + + + I + G+ + GD LR+E ++ + + +E+ R I+G LP + Sbjct: 71 DKIHNNPKIDKVGLKESVGDVYLRDE----KKINITKFNENALRLLPYKAIKGELPKKHD 126 Query: 136 EVVLNTWVMDKLGIPHSLGQKATLDIDIGERVVRQDFIVSGYY--DADKNLSIAGLAFVS 193 E+ L +W++ + ++G + + I + R ++F ++G ++ L GL V Sbjct: 127 EIALESWILSYMDGTPNVG--SIIKIKLNNRE-EKEFKITGLIKNNSSSQLEGRGLGIVY 183 Query: 194 EAWVQKNLSSIDPVKSKASGSYVNTTELSVMFSNAVDIEKKLNKVLADTGLDVPIGINWA 253 N S I S+ + +EL F E+ L L G + Sbjct: 184 SDKFNINNSIIYATISEKADISDTISELKGNFKEFKSNEEVLR--LMGEGQN-------- 233 Query: 254 YTTSSLTDNITKVVPYLAVILI--IMLSGYLLIYNIFYISVVRDVKFYGLLKAIGTTPRQ 311 +NI K + +A I+I ++++ +IYN F ISVV +K +GLL+A+G T +Q Sbjct: 234 -------ENINKSLYQIASIIIGVVVIATIAVIYNSFQISVVERIKQFGLLRAVGATQKQ 286 Query: 312 LKKIIT-----IQSQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVETSYSNSPWIFI 366 +KKI+ I S V SY I Sbjct: 287 IKKIVLREATLISSIGIPLGLFFGVIALKIVAEIFKMMSSSAFGNLNVVISYK----ILA 342 Query: 367 GGAVFAYLTVWIAASKPGRMAARIAPVEAVRFAGVSAKRKVNK-RSKRGAKLYG----MA 421 A+ +++I+A P R A +I+P+ A+ + K K+ K R + K+ G MA Sbjct: 343 ISALVGLFSIYISALIPARFAGKISPLVAISSRALIVKEKIKKNRGRIAKKILGINGLMA 402 Query: 422 FANLFRNKKKXXXXXXXXXXXXXXXXXXXXVISSLDVNKYLSTFLSGDMVIRNKAIVLSA 481 F N+ RNKK+ + S+ + + +TF+ N + Sbjct: 403 FKNIKRNKKRFNITVFS-------------ITISVTLFIFFTTFI-------NMLNFFNT 442 Query: 482 GEQSGDPYKLSGPFISKLGKIDGVER-------VDSVYFKFENYTID---DTIRAALKPL 531 E D + S +G +D + +D + K NYT D I + K + Sbjct: 443 PETESDISQFS-----VVGTVDNKGKSSLNKNIIDQI--KNNNYTNDVFVHYINYSSKSV 495 Query: 532 AEGAPSEPALTWVFKNNFIQLDLYG------------IDAGWYELVQKDVLEGSFDPQKF 579 EP L V + + ++DL G D + K V+ G D K Sbjct: 496 ISIDKKEPNLEKVAPDIYEKIDLDGKEMIKLGVAFDAYDENKLKNSSKYVVSGKIDIDKM 555 Query: 580 ASGNYVLVT----------------TSIKAGDTDKSYYHPGDHIEYKGLGKSYEVMAVLN 623 N V++ T +K GD S+Y + + +GK+ E LN Sbjct: 556 KKENGVILVKNQLIADKGGHYNGSFTKLKVGD---SFYVNKNILRDDLIGKNNEKENKLN 612 Query: 624 YNALYAATTQVYS-----PYGYNAFFPSSE----LTRKLPAG--SGPPMALSATLHVDPA 672 +N +V + PYG FF + E +T K G +G M + A Sbjct: 613 FNKEDMVKVKVVAIVNTPPYG---FFVNPEHLKIITTKDVLGNIAGKDMNKILLDQIQSA 669 Query: 673 KLDSVMQTTKSLAASSE--------------ELVFKSREDYRQELGGF-IRIFQTVGYGL 717 ++D K A E ++ ++ Q+ G I+I YG Sbjct: 670 EID-----LKDKGAGDEFQKWIQPLGDRFGIRVINHVQQMEEQKSSGLQIKILM---YGF 721 Query: 718 SFVIALIGVLNYINTVITGVIARRNEFALLESIGMTKPQLKKVLVYEG-LFNVLLTVSII 776 V++LIG +N INT+ T +I R+ E A L +IGMT +K++++ EG L+ V ++ + Sbjct: 722 IVVVSLIGAVNIINTITTNLILRKKEIASLSAIGMTYKNIKRMILTEGILYGVYGSIYGV 781 Query: 777 STLGVFLTYSISKSIADSMAFTVFHMGWLPFILAVPVLAAIAYTVTLSSYRMLSKDTIVE 836 +G FL+Y++ ++ + M F + + W +AV I + M+ K I++ Sbjct: 782 -IVGTFLSYAMFSNMTNIMKFK-WPIPWQSICIAVGTSIFIGLISVIRPLNMIKKSNIID 839 Query: 837 RLRQTE 842 ++ E Sbjct: 840 VIKAEE 845 >ref|ZP_00100370.1| COG0577: ABC-type antimicrobial peptide transport system, permease component [Desulfitobacterium hafniense] Length = 176 Score = 97.1 bits (240), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 60/170 (35%), Positives = 90/170 (52%), Gaps = 9/170 (5%) Query: 225 FSNAVDIEKKLNKVLADTGLD--------VPIGINWAYTTSSLTDNITKVVPYLAVILII 276 F+N+++I + L KV+ ++G + G+NWAY ++ + + + + +L+ Sbjct: 7 FANSLNIAENLEKVVTESGFSMEEGDPNYLATGVNWAYLSTVVGMDAGTLTALASALLLF 66 Query: 277 MLSGYLLIYNIFYISVVRDVKFYGLLKAIGTTPRQLKKIITIQS-QXXXXXXXXXXXXXX 335 +L+GYL+IYNIF ISV+RD++FYGLLK IGTT RQL+ II Q+ + Sbjct: 67 VLTGYLIIYNIFQISVLRDIRFYGLLKTIGTTGRQLRAIIRRQALRLSLIGIPLGLLAGF 126 Query: 336 XXXXXXXXXXXXXXXXXXVETSYSNSPWIFIGGAVFAYLTVWIAASKPGR 385 S S P IF G A+FA TVWI+A KPG+ Sbjct: 127 LVGKALVPYLMERSSYAGSGVSVSPDPLIFAGAALFALFTVWISAHKPGK 176 >ref|NP_782039.1| putative permease [Clostridium tetani E88] gb|AAO35976.1| putative permease [Clostridium tetani E88] Length = 875 Score = 89.4 bits (220), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 88/344 (25%), Positives = 154/344 (44%), Gaps = 45/344 (13%) Query: 123 VNFIEGGLPAAENEVVLNTWVMDKLGIPHSLGQKATLDIDIGERVVRQDFIVSGYYD--- 179 +N EG LP ENE+VL WV D L +G K LDI G+R+V++ G + Sbjct: 128 INVKEGRLPQKENEIVLEYWVPDYLSGKPKIGDKIKLDI--GKRIVKEKGSKDGEGETKE 185 Query: 180 -----ADKNLSIAGLAFVSEAW----VQKNLSSIDPVKSKASGSYVNTTELSVMFSNAVD 230 + +I G+ +W + + ++ +D K + Y + V ++A D Sbjct: 186 TFNKTGKREYTIVGVTAPKFSWPGVYITQGITFLDENKLASDKKY----NVYVKLNSAKD 241 Query: 231 IEKKLNKVLADTGLDVP------IGINWAYTTS-----------SLTDNITKVVPYLAVI 273 + KK + + G++ G N Y S+ + +T +V + ++ Sbjct: 242 VYKKCENIAKNLGMEKEKTSKGIYGYNIEYNEQLLRLYAQSLDKSINEGLTALVAF--IV 299 Query: 274 LIIMLSGYLLIYNIFYISVVRDVKFYGLLKAIGTTPRQLKKIITIQSQXXXXXXXXXXXX 333 +I++S +IYNIF ISV+ V +G+L+ G P Q+KK++ ++ Sbjct: 300 GLIIISTIAVIYNIFNISVLERVSQFGILRCTGAAPNQIKKLVLKEALILSFIGIPLGLA 359 Query: 334 XXXXXXXXXXXXXXXXXXXXVETSYSNSPWIFIGGAVFAYLTVWIAASKPGRMAARIAPV 393 ++ SP +FI A+ +T++++A P R A++++P+ Sbjct: 360 NGVLAMKIVISVVKVLLKDEIKVVI--SPVVFIISAIIGLITIYLSAIGPARKASKVSPL 417 Query: 394 EAVRFAGVSAKRKVNKRSKRGA--KLYG----MAFANLFRNKKK 431 EAVR G K K KR + K++G +A+ NL RN+KK Sbjct: 418 EAVRNTGSLKKEKFKKRKRFRIINKVFGIEGEIAYKNLKRNRKK 461 Score = 60.8 bits (146), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 39/133 (29%), Positives = 74/133 (55%), Gaps = 11/133 (8%) Query: 715 YGLSFVIALIGVLNYINTVITGVIARRNEFALLESIGMTKPQLKKVLVYEGLFNVLLTVS 774 YG VI LIG LN +NT+ T +I R+ E A++ ++GM + ++ K++ EG++ ++ Sbjct: 749 YGFVGVITLIGCLNIVNTISTNLILRKRELAMIRAVGMDRGKMSKMICIEGIYYGIIASI 808 Query: 775 IISTLGVFLTYSISKSIADSMAFTVFHMGWLPFI--LAVPVLAAIAYTVTLSSY---RML 829 +G L+Y + K + + F W+ I + + V+ AI ++ +S+Y R + Sbjct: 809 YGGIIGTALSYELFKIMTNFRDFQ-----WVFPIKEILIAVIGAIIISL-ISTYIPLRKI 862 Query: 830 SKDTIVERLRQTE 842 +K+ I+E +R E Sbjct: 863 NKENIIENIRGAE 875 >ref|NP_783744.1| ABC transporter-associated permease [Clostridium tetani] gb|AAO37412.1| ABC transporter-associated permease [Clostridium tetani] Length = 817 Score = 81.6 bits (200), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 152/743 (20%), Positives = 307/743 (41%), Gaps = 64/743 (8%) Query: 64 DFHGGFKYLTPAQAETLKKHSSIREYGVSLNAGDLRNEVFKDNRVEVKQIDESYARHSFV 123 D++ + ETLK + +++ V + G ++ K + + ++ DE++ + + + Sbjct: 56 DYYADLNTKNKQKTETLKGYKYLKDKTVHIPYGMFTSKDMKTS-LNIEISDETFLKSTGL 114 Query: 124 NFIEGGLPAAENEVVLNTWVMDKLGIPHSLGQKATLDIDIGERVVRQDFIVSGY--YDAD 181 ++G P ++E+ ++ ++ + I L Q TLD+ + ++ D GY Y Sbjct: 115 EMLKGRFPKNKDEICVDESFLENMNITKKLDQYITLDLINSPKNLKSD----GYKEYKFT 170 Query: 182 KNLSIAGLAFVSEAWVQKNLS-------SIDPVKSKASGSYVNTTELSVMFSNAVDIEKK 234 K + G+ + K +S +ID + ++ + + +I K Sbjct: 171 KKFKLVGILKTNPGIKFKGVSCGILSIKNIDDIPKDFIDYHILIKTFP--YKSVEEITKS 228 Query: 235 LNKVLADTGLDVP-IGINWAYTTSSLTDNITKVVPYLAVILIIMLSGYLLIYNIFYISVV 293 + + ++ I IN N +K++ +A ++++++ L IYNIF +S++ Sbjct: 229 IEHIQNRFNINEDDIFINRGLMQVIKHSNDSKILS-IAFEMVVIIASILTIYNIFNMSIL 287 Query: 294 RDVKFYGLLKAIGTTPRQLKKIITIQSQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 353 K YG+L+ +G +++ I+ + Sbjct: 288 DRTKDYGILRCLGFRKKKIMSIVLKEGLILSIISIPIGILIGCFLSKSIYFSIGKILNNT 347 Query: 354 VETSYSNSPWIFIGGAVFAYLTVWIAASKPGRMAARIAPVEAVRFAGVSAK-RKVNKRS- 411 V+ S + S ++ + + L+V I+A P A ++P+ A++ V +K K+ R Sbjct: 348 VK-SLTLSIYVILLVFIITLLSVSISAFMPAIYAYNVSPIGAIKNLNVYSKIHKIKHRKG 406 Query: 412 ----KRGAKLYGM-AFANLFRNKKKXXXXXXXXXXXXXXXXXXXXVISSLDVNKYLSTFL 466 K+ K GM A+ N++R KK+ + + + F Sbjct: 407 HFIIKKLFKNEGMLAYKNIWRMKKRTYLIVFSLGISITIFTAFMYYFNHIAIEDPSENF- 465 Query: 467 SGDMVIRNKAIVLSAGEQSGDPYKLSGPFISKLGKIDGVERVDSVYFKFENYTIDDT-IR 525 +N L+ + + Y FISK+ + G++ + + E ++++ I+ Sbjct: 466 ------KNGQFKLTFDNRLDNTYCYDNNFISKINNMHGIKHITKIQSATETAVLNESFIK 519 Query: 526 AALKPLAEGAPSEPALTWVFKNNFIQLDLYGIDA---GWYELVQKDV-LEGSFDPQKFAS 581 + LK PSE LT I Y + G+ E + KD+ + D + Sbjct: 520 SNLK----DNPSENNLT-------IDNGEYRVPCNVIGYNEEIVKDLKTKIGKDEVILIN 568 Query: 582 GNYVLVTTSIKAGD--TDKSYYHPGDHIEYKGLGKSYEVMAVLNYNALYAATTQVYSPYG 639 Y T++KAGD T + +Y K K +++ +LN A Y + S G Sbjct: 569 DKYAKNNTNLKAGDIITLRRFYKDNIEQRVKYEDKEFKIKDILN-KAPYQS-----SMIG 622 Query: 640 YNAFFPSSELTRKLPAGSGPPMALSATLHVDPAKLDSVMQTTKSLAASSEELVFKSREDY 699 +A + + +S + + +++ ++ S+ L + Y Sbjct: 623 ISALIRHDDFKNFTKINGYRTLDISVYENFNMDFINNELENLTKEVKYSKLLSYYKEIKY 682 Query: 700 RQELGGFIRIFQTVGYGLSFVIALIGVLNYINTVITGVIARRNEFALLESIGMTKPQLKK 759 ++ FI+I + Y I LIG+LN INT+ T ++ R +EFA L++IG K ++K Sbjct: 683 IKDSIKFIKI---LTYSFIACIVLIGLLNIINTINTNIVLRMSEFATLKAIGFNKNHIRK 739 Query: 760 VLVYEGLFNVLLTVSIISTLGVF 782 V+++EGLF +L+ S G+F Sbjct: 740 VIMFEGLFYGILS----SIFGMF 758 >ref|NP_347094.1| Predicted ATP transporter permease component [Clostridium acetobutylicum] pir||G96955 probable ATP transporter permease component CAC0454 [imported] - Clostridium acetobutylicum gb|AAK78434.1| Predicted ATP transporter permease component [Clostridium acetobutylicum] Length = 832 Score = 81.3 bits (199), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 180/749 (24%), Positives = 299/749 (39%), Gaps = 79/749 (10%) Query: 127 EGGLPAAENEVVLNTWVMDKLGIPHSLGQKATLDIDIGERVVRQDFIVSGYYDADKNLSI 186 +G P VVL T + +KLG + K + + G + ++FI+SG+ D + N Sbjct: 130 KGTFPKDNKHVVL-TKMFNKLGYKVNDKIKIYVLTNGGGDLKEKEFIISGFLDYNYNT-- 186 Query: 187 AGLAFVSEAWVQKNLSSIDPVKSKASGSYVNTTELSVMFSNAVDIEK--KLNKVLADTGL 244 + F E V + L+ ID K S+VN + + IEK K V+ + GL Sbjct: 187 --ILFPKE--VAEKLTPID----KKYISFVNMKKGVNYNKSFRTIEKQIKCEDVMYNYGL 238 Query: 245 DVPIGINWAYTTSSLTDNITKVVPYLAVILIIMLSGYLLIYNIFYISVVRDVKFYGLLKA 304 + + Y + + V+ L V+ I++S L++Y+ F IS+ VK +G+L++ Sbjct: 239 ---LELEGNYNNKASIYGMAFVMFILGVV--ILVSSALVVYSAFNISIFERVKEFGILRS 293 Query: 305 IGTTPRQLKKIITIQSQXXXXXXX-XXXXXXXXXXXXXXXXXXXXXXXXXVETSYSNSPW 363 +G + Q+K +I I++ V+ + S Sbjct: 294 VGASNYQIKSVIFIEAFIMIILGIPLGVLCGIAAIKILFLIVTLSKFSMFVDINIYISSR 353 Query: 364 IFIGGAVFAYLTVWIAASKPGRMAARIAPVEAVRFAGVSAKRKVNKRSKRG--AKLYGMA 421 + + +F L + ++ P + I P+EA+R G + +NKRS K+ Sbjct: 354 VIVSSIIFGVLCIIFSSIVPVIKSGGINPIEAIR--GTGKTKALNKRSYFNIIKKINFQT 411 Query: 422 FANLFRNKKKXXXXXXXXXXXXXXXXXXXXVISSLDVNKYLSTFLSGDMVIRNKAIVLSA 481 F FRN K+ V +S L F+ G + I++ I Sbjct: 412 FLT-FRNIKRNKGRFRLVVSSVAVSVVIFIVFNSFMF--LLFQFMKGPLYIKDFNI---- 464 Query: 482 GEQSGDPYKLSGPFISKLGKIDGVERVDSVYFKFENYTIDDTIRAALKPL----AEGAPS 537 S I + I GV+ V Y +++T R P E + Sbjct: 465 ---SSRDITFKKEDIYAIKDIKGVKDV---------YPMEETSRLIFCPFNRLSKEFKQA 512 Query: 538 EPALTWVFKNNFI--QLDLY--GIDAGWYELVQKDVLEGSFDPQKFASGNYVLVTTSIK- 592 L K+ + D+Y G D V+ V++G D +K SG VL IK Sbjct: 513 NKKLEH--KDGYFWSGADIYFAGYDLNELNEVKDYVIKGKIDIEKLNSGEEVLAFEKIKY 570 Query: 593 ---AGDTDKSYYHPGDHI---EYKGLGKSYEVMAV-LNYNALY-----AATTQVYSPYGY 640 + +++ + GD I + GK+ A L +N L A ++ S Y Sbjct: 571 RGVGEEINETNLNIGDEIYIDNNRVYGKADPAKASKLKFNKLKKVKVGAIVSKNLSYYNN 630 Query: 641 NA--FFPSSELTRKLPAGSGPPMALSATLHVDP-AKLDSVMQTTKSLAASSEELVFKSRE 697 N+ FF SS T G + VD A + V + S A + + + + E Sbjct: 631 NSAMFFISS--TEGYKNIIGHDSFDEFQVEVDKGANKEEVRKKIDSKLAVNYKYILQDEE 688 Query: 698 DYRQELGGFIRIFQTVGYGLSFVIALIGVLNYINTVITGVIARRNEFALLESIGMTKPQL 757 + + + + YG VI+LIG+LN INTV T ++ R EFA L S+GM+ L Sbjct: 689 EIESQYKQYKIETIVLVYGFIAVISLIGILNVINTVNTNLMLRLKEFACLISVGMSIHDL 748 Query: 758 KKVLVYEGLFNVLLTVSIISTLGVFLTYSISKSIADSMAFTVFHMGWLPFILAVPV---- 813 KK+L+ EG+ V +G L Y I K + ++ + W FI + V Sbjct: 749 KKMLLTEGILYGAYGVFYGLLVGSGLYYLIYKEMMNTSV-----VRWSLFIKEIAVATIG 803 Query: 814 LAAIAYTVTLSSYRMLSKDTIVERLRQTE 842 + I + + + + K I+E +R E Sbjct: 804 VIVIIFISMIFPLKRIQKMNIIENMRGDE 832 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,088,902,620 Number of extensions: 43230994 Number of successful extensions: 115229 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 115250 Number of HSP's successfully gapped: 12 Length of query: 842 Length of database: 757,237,578 Length adjustment: 134 Effective length of query: 708 Effective length of database: 393,414,312 Effective search space: 278537332896 Effective search space used: 278537332896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)