BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0009.p (179 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00183615.1| hypothetical protein Exigu093901 [Exiguobacte... 264 6e-70 ref|NP_600237.1| hypothetical protein NCgl0971 [Corynebacterium ... 185 4e-46 ref|NP_630351.1| putative reductase [Streptomyces coelicolor A3(... 181 5e-45 ref|NP_739340.1| conserved hypothetical protein [Corynebacterium... 162 3e-39 gb|AAP69587.1| putative reductase [Streptomyces griseoflavus] 157 7e-38 >ref|ZP_00183615.1| hypothetical protein Exigu093901 [Exiguobacterium sp. 255-15] Length = 180 Score = 264 bits (675), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 129/180 (71%), Positives = 148/180 (82%), Gaps = 1/180 (0%) Query: 1 MSALNIGIILGSTRQGRLSPQVGEWVKGIADQRGDANYEIVDIADFKLPLLGEADA-SEQ 59 M+ LNIGIILGSTR+GRLSPQVG WVK +AD+RGDA+Y I+DIAD+KLPLLGE D + Sbjct: 1 MTKLNIGIILGSTREGRLSPQVGNWVKELADKRGDAHYTIIDIADYKLPLLGEKDGDASG 60 Query: 60 AGRWNDKLNALDGFVFIVAEYNHSITGALKNALDYAREAWNNKAAGIVSYGSVGGARAAE 119 A W++ + DGFVFI EYNHSI+ +LKNALDY R WNNKAAGIVSYGSVGGARAAE Sbjct: 61 AAAWSEAIAVQDGFVFITQEYNHSISASLKNALDYLRVEWNNKAAGIVSYGSVGGARAAE 120 Query: 120 HLRGILGELSIADVRIHPALSLFTDFENGQTFKPAELHLTNVNGMLDQVVAWSGALKTLR 179 HLRGILGEL IADVR+HPALSLFTDFENG K A + +VN MLDQV+ W+GALKT+R Sbjct: 121 HLRGILGELLIADVRVHPALSLFTDFENGAVLKAAPVQADSVNQMLDQVIPWAGALKTIR 180 >ref|NP_600237.1| hypothetical protein NCgl0971 [Corynebacterium glutamicum ATCC 13032] dbj|BAB98407.1| Predicted flavoprotein [Corynebacterium glutamicum ATCC 13032] emb|CAF19716.1| NADPH-dependent FMN reductase [Corynebacterium glutamicum ATCC 13032] Length = 188 Score = 185 bits (470), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 96/181 (53%), Positives = 118/181 (65%), Gaps = 8/181 (4%) Query: 6 IGIILGSTRQGRLSPQVGEWVKGIADQRGDANYEIVDIADFKLPLLGEA-------DASE 58 I II GSTR GR++ V WV A +R DA YE+VDIADF P+L EA A+E Sbjct: 4 IAIITGSTRPGRVNIDVANWVLERAQERNDAQYELVDIADFNFPVLDEAMPAGYGQYANE 63 Query: 59 QAGRWNDKLNALDGFVFIVAEYNHSITGALKNALDYAREAWNNKAAGIVSYGSVGGARAA 118 W K+ DGF+F+ EYNHS+ AL NAL Y WNNKAAGIVSYGS G RAA Sbjct: 64 HTKAWAAKIAEFDGFIFVTGEYNHSVPAALTNALSYLSAEWNNKAAGIVSYGSAMGVRAA 123 Query: 119 EHLRGILGELSIADVRIHPALSLFTDFENGQTFKPAELHLTNVNGMLDQVVAWSGALKTL 178 EHLRGIL EL IA V+ LS+FTDFE FKP+E +++V+ ML+Q+V W+ A+ T+ Sbjct: 124 EHLRGILSELQIAHVQKTGLLSIFTDFEY-PNFKPSEQGISSVDAMLEQLVVWTKAMSTI 182 Query: 179 R 179 R Sbjct: 183 R 183 >ref|NP_630351.1| putative reductase [Streptomyces coelicolor A3(2)] emb|CAB60170.1| putative reductase [Streptomyces coelicolor A3(2)] Length = 185 Score = 181 bits (460), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 93/182 (51%), Positives = 116/182 (63%), Gaps = 7/182 (3%) Query: 5 NIGIILGSTRQGRLSPQVGEWVKGIADQRGDANYEIVDIADFKLPLLGE------ADASE 58 + I++GST R+ V WV +A +RGDA +E+VD+ D+ LP L E S+ Sbjct: 3 EVAIVIGSTGPRRVGEAVARWVHELAAKRGDAEFELVDLKDYDLPHLDEMMPAAMGQYSQ 62 Query: 59 QAGR-WNDKLNALDGFVFIVAEYNHSITGALKNALDYAREAWNNKAAGIVSYGSVGGARA 117 R W +K+ D FVF+ EYNHS TGALKNA+D+ W+NKAAG VSYGS GG RA Sbjct: 63 PHTRAWAEKVAGFDAFVFVTPEYNHSTTGALKNAIDFLHAEWHNKAAGFVSYGSNGGVRA 122 Query: 118 AEHLRGILGELSIADVRIHPALSLFTDFENGQTFKPAELHLTNVNGMLDQVVAWSGALKT 177 EHLR +LGEL +ADV+ ALS+FTDFE+ FKP E H +N ML QVVAW AL Sbjct: 123 VEHLRLVLGELQVADVQAQVALSVFTDFEHFTAFKPGEHHEATLNTMLAQVVAWGEALAP 182 Query: 178 LR 179 LR Sbjct: 183 LR 184 >ref|NP_739340.1| conserved hypothetical protein [Corynebacterium efficiens YS-314] dbj|BAC19540.1| conserved hypothetical protein [Corynebacterium efficiens YS-314] Length = 185 Score = 162 bits (410), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 85/181 (46%), Positives = 107/181 (59%), Gaps = 7/181 (3%) Query: 6 IGIILGSTRQGRLSPQVGEWVKGIADQRGDANYEIVDIADFKLPLLGEA-------DASE 58 I I++GSTR R + V EWV A + A YE+VDIADF LPLL E + + Sbjct: 4 IAIVVGSTRPNRNAASVAEWVFDNAPKDSGAEYELVDIADFNLPLLDEPMPPAMGQYSKD 63 Query: 59 QAGRWNDKLNALDGFVFIVAEYNHSITGALKNALDYAREAWNNKAAGIVSYGSVGGARAA 118 W +K+ + DGF+F+ EYNHS+ ALKNA+D+ WNNKA G VSYGS GG RA Sbjct: 64 HTKAWAEKVASFDGFIFVTPEYNHSVPAALKNAVDFLYAEWNNKAIGFVSYGSAGGTRAV 123 Query: 119 EHLRGILGELSIADVRIHPALSLFTDFENGQTFKPAELHLTNVNGMLDQVVAWSGALKTL 178 E R I EL +ADVR L+ DF + TF+P+E + + QV AWS ALKTL Sbjct: 124 EAWRLIAAELQMADVRAQVFLTFGNDFADMSTFQPSEGSTGALQDLFSQVTAWSEALKTL 183 Query: 179 R 179 R Sbjct: 184 R 184 >gb|AAP69587.1| putative reductase [Streptomyces griseoflavus] Length = 213 Score = 157 bits (398), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 87/198 (43%), Positives = 112/198 (56%), Gaps = 22/198 (11%) Query: 4 LNIGIILGSTRQGRLSPQVGEWVKGIADQR-----GDANYEIVDIADFKLPLLGEADAS- 57 + I +ILGSTR GR V +WV +A + G+A +E+VD+A++ LPLL E + Sbjct: 2 IRIAVILGSTRPGRRGAVVAQWVAEVAARHPAAVMGEAEFELVDLAEYGLPLLDEPVPAM 61 Query: 58 ------EQAGRWNDKLNALDGFVFIVAEYNHSITGALKNALDYAREAWNNKAAGIVSYGS 111 E+ RW + + DGFVF+ EYNHS+ ALKNA+D+ W +KAAG VSYG Sbjct: 62 FGQYQKEETRRWAAAIGSFDGFVFVTPEYNHSVPAALKNAIDHLFAEWTDKAAGFVSYGV 121 Query: 112 VGGARAAEHLRGILGELSIADVRIHPALSLFTDFENGQT----------FKPAELHLTNV 161 GG RA EHLR L E+ +A VR LS+F DF+ F P V Sbjct: 122 HGGTRAVEHLRLALAEVKVAGVRSQVVLSVFNDFDYTGCDMTDPTAMGRFTPGPQQEQTV 181 Query: 162 NGMLDQVVAWSGALKTLR 179 N MLD+VVAWS ALK LR Sbjct: 182 NTMLDEVVAWSTALKPLR 199 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 270,597,119 Number of extensions: 10675134 Number of successful extensions: 22670 Number of sequences better than 1.0e-05: 80 Number of HSP's gapped: 22646 Number of HSP's successfully gapped: 80 Length of query: 179 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 61 Effective length of database: 436,855,896 Effective search space: 26648209656 Effective search space used: 26648209656 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 122 (51.6 bits)