BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0008.p (315 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_644302.1| integral membrane protein [Xanthomonas axonopod... 91 5e-17 ref|NP_810310.1| putative membrane protein [Bacteroides thetaiot... 84 5e-15 ref|NP_746873.1| transporter, putative [Pseudomonas putida KT244... 76 9e-13 ref|NP_253471.1| conserved hypothetical protein [Pseudomonas aer... 74 4e-12 ref|ZP_00126172.1| COG0697: Permeases of the drug/metabolite tra... 73 7e-12 >ref|NP_644302.1| integral membrane protein [Xanthomonas axonopodis pv. citri str. 306] gb|AAM38838.1| integral membrane protein [Xanthomonas axonopodis pv. citri str. 306] Length = 298 Score = 90.5 bits (223), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 77/265 (29%), Positives = 115/265 (43%), Gaps = 21/265 (7%) Query: 24 WGGTYVGMKVAIE--TMPPFLMAGIRFFVAGAVLYIISRLSGAKRPAGREWRSSAIVXXX 81 WG TY+ ++ A+E P +++G RF VAG+VLY R G P +W++ A++ Sbjct: 27 WGSTYLAIRFALEGGAQPLTMVSGARFIVAGSVLYSALRWRGVAAPTRAQWKNVALMGAA 86 Query: 82 XXXXXXXXVAWSEQRXXXXXXXXXXXXXXXWMMLFGWL----SRSGKRPXXXXXXXXXXX 137 V +E+ WM LFG L + G+ Sbjct: 87 LLLLGNGMVVLAERSVSSGLAATAVASVPLWMALFGALRGQHASGGEWLGIVIGFLGVVW 146 Query: 138 XXXXXXXXFQPGHKDNGTATDLTGIITXXXXXXXXXXXXMYSRSARMPDSPLMSTAAQML 197 PG G++ ++SR +P SP M+ A QML Sbjct: 147 LNAGSSLTATPG-----------GLVLLLIAPIGWAFGSVWSRGRDLP-SPFMTAAGQML 194 Query: 198 TGGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFGSIIGYTAYIWLLKNADAALVS 257 GGVLL+ + ++ P S + A+ YL VFGSI+ +TAY+WLL + AL Sbjct: 195 CGGVLLVSTGLLIGERPQV-WP--SPQGLLAVAYLCVFGSIVAFTAYVWLLHHVRPALAG 251 Query: 258 TYAFVNPVVAVFFGWLLAGEQLTVN 282 +YA+VNPV+AV G L E+ + Sbjct: 252 SYAYVNPVIAVSLGAWLGHERFSAQ 276 >ref|NP_810310.1| putative membrane protein [Bacteroides thetaiotaomicron VPI-5482] gb|AAO76504.1| putative membrane protein [Bacteroides thetaiotaomicron VPI-5482] Length = 315 Score = 84.0 bits (206), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 73/272 (26%), Positives = 116/272 (42%), Gaps = 9/272 (3%) Query: 15 IALVSVYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISRLSGAKRPAGREWRS 74 IA +Y+ WG TY + VA++ PFL+ +RF AG +L I L G + + Sbjct: 16 IAYFLIYVVWGSTYFFIGVALKDFSPFLLGALRFTTAGLILLGICYLRGEQIFKKSLVKR 75 Query: 75 SAIVXXXXXXXXXXXVAWSEQRXXXXXXXXXXXXXXXWMMLFG---WLSRSGKRPXXXXX 131 SA+ V +++ W++L W R+ R Sbjct: 76 SAVSGIVLLFVDMAVVMLAQRYLTSSLVAIIASSTALWILLLDVPMW--RTNFRNPLTIA 133 Query: 132 XXXXXXXXXXXXXXFQPGHKDNGTATDLTGIITXXXXXXXXXXXXMYS--RSARMPD-SP 188 Q + ++ GI+ +Y+ RS+R + + Sbjct: 134 GGGIGFGGVVMLYAEQLNAEWLHPQSE-RGILLLVFGCISWALGTLYAKYRSSREEEVNA 192 Query: 189 LMSTAAQMLTGGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFGSIIGYTAYIWLL 248 +A QML V+ I + D + D+ +S+ S+ +L YLI FGS++ Y+AY+WLL Sbjct: 193 FAGSAWQMLFASVMFWICAVVNGDVREADLSTVSVTSWFSLLYLISFGSLLAYSAYVWLL 252 Query: 249 KNADAALVSTYAFVNPVVAVFFGWLLAGEQLT 280 K AA V T+A+VNP +AV G EQ+T Sbjct: 253 KVRPAAEVGTHAYVNPFIAVLLGVSFGNEQVT 284 >ref|NP_746873.1| transporter, putative [Pseudomonas putida KT2440] gb|AAN70337.1| transporter, putative [Pseudomonas putida KT2440] Length = 306 Score = 76.3 bits (186), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 41/103 (39%), Positives = 68/103 (66%), Gaps = 4/103 (3%) Query: 177 MYSRSARMPDSPLMSTAAQMLTGGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFG 236 ++S++ +P P M++AA+ML GG +LL+ S + + +P + + AL YL+ FG Sbjct: 169 VWSKTLPLPQGP-MASAAEMLVGGAVLLLGSVLSGE-RMTQLPTAA--GWGALAYLVFFG 224 Query: 237 SIIGYTAYIWLLKNADAALVSTYAFVNPVVAVFFGWLLAGEQL 279 SI+ ++AY++LLK+ A ++YA+VNP VAV G + AGEQ+ Sbjct: 225 SILAFSAYMYLLKHVRPAAATSYAYVNPAVAVLLGIVFAGEQI 267 Score = 60.8 bits (146), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 28/70 (40%), Positives = 46/70 (65%), Gaps = 1/70 (1%) Query: 9 PVYVIGIALVSVYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISRLSGAKRPA 68 P+ +IG A +++YL WG TY+ +++ +E+ PP LMAG+RF +AG +LY R G P Sbjct: 8 PLLLIG-AFLALYLVWGSTYLFIRIGVESWPPMLMAGVRFLIAGGLLYGFLRWRGVPAPT 66 Query: 69 GREWRSSAIV 78 +WR++ + Sbjct: 67 WPQWRAAGAI 76 >ref|NP_253471.1| conserved hypothetical protein [Pseudomonas aeruginosa PA01] pir||E83049 conserved hypothetical protein PA4783 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG08169.1| conserved hypothetical protein [Pseudomonas aeruginosa PAO1] Length = 296 Score = 73.9 bits (180), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 46/104 (44%), Positives = 70/104 (67%), Gaps = 6/104 (5%) Query: 177 MYSRSARMPDSPLMSTAAQMLTGGVLLLIFSYFTSDWSKLDI-PAISLRSYAALGYLIVF 235 ++SR +P P M++AA+ML GG +LL+ S + + +L+ P+ S + AL YLI+F Sbjct: 168 VWSRRLSLPAGP-MASAAEMLVGGAVLLVGSLASGE--RLEHWPSTS--GWLALVYLILF 222 Query: 236 GSIIGYTAYIWLLKNADAALVSTYAFVNPVVAVFFGWLLAGEQL 279 GSII ++AY +LL+N A ++YA+VNP VAV G L AGE++ Sbjct: 223 GSIIAFSAYQYLLRNVRPAAATSYAYVNPAVAVLLGTLFAGERI 266 Score = 61.6 bits (148), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 28/70 (40%), Positives = 44/70 (62%), Gaps = 1/70 (1%) Query: 9 PVYVIGIALVSVYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISRLSGAKRPA 68 P+ + G A ++Y WG TY+ ++ +E+ PP LMAG+RF +AG +++ R GA P Sbjct: 7 PLLLFG-AFFALYFIWGSTYLAIRFGVESWPPMLMAGVRFVIAGGLMFAWLRWRGAPLPT 65 Query: 69 GREWRSSAIV 78 REWR + +V Sbjct: 66 WREWRGAGVV 75 >ref|ZP_00126172.1| COG0697: Permeases of the drug/metabolite transporter (DMT) superfamily [Pseudomonas syringae pv. syringae B728a] Length = 304 Score = 73.2 bits (178), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 43/104 (41%), Positives = 65/104 (62%), Gaps = 4/104 (3%) Query: 179 SRSARMPDSPLMSTAAQMLTGGVLLLIFSYFTSDWSKLDIPAISLRSYAALGYLIVFGSI 238 S+ +P P M++AA+M+T G LLI S + + +P + + AL YL+VFGSI Sbjct: 171 SKHLPLPKGP-MASAAEMITAGATLLIGSALSGE-RLAHMPTAA--GWGALLYLVVFGSI 226 Query: 239 IGYTAYIWLLKNADAALVSTYAFVNPVVAVFFGWLLAGEQLTVN 282 + ++AY++LLKN A ++YA+VNP VAV G + AGE + V Sbjct: 227 VAFSAYMYLLKNVRPAAATSYAYVNPAVAVMLGVVFAGETIGVE 270 Score = 63.9 bits (154), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 29/78 (37%), Positives = 51/78 (65%), Gaps = 2/78 (2%) Query: 1 MKQKAGANPVYVIGIALVSVYLFWGGTYVGMKVAIETMPPFLMAGIRFFVAGAVLYIISR 60 M Q +P+ +IG A +++YL WG TY+ +++ +E+ PP +MAG+RF +AG ++Y R Sbjct: 1 MPQSVRVSPL-LIG-AFLALYLIWGSTYLVIRIGVESWPPLMMAGVRFLIAGCLMYGFLR 58 Query: 61 LSGAKRPAGREWRSSAIV 78 G P REW+++ ++ Sbjct: 59 FRGVPAPTWREWKAAFVI 76 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 343,296,927 Number of extensions: 10926545 Number of successful extensions: 31658 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 31664 Number of HSP's successfully gapped: 47 Length of query: 315 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 190 Effective length of database: 417,850,203 Effective search space: 79391538570 Effective search space used: 79391538570 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)