BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0471.0002.p (451 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00019229.1| hypothetical protein [Chloroflexus aurantiacus] 119 1e-25 gb|AAO83404.1| putative SN-glycerol-3-phosphate-binding periplas... 115 2e-24 ref|ZP_00036882.1| COG1653: ABC-type sugar transport system, per... 109 1e-22 ref|NP_738126.1| putative glycerol-3-phosphate-binding periplasm... 108 3e-22 ref|NP_600604.1| ABC-type transporter, periplasmic component [Co... 107 4e-22 >ref|ZP_00019229.1| hypothetical protein [Chloroflexus aurantiacus] Length = 448 Score = 119 bits (299), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 119/476 (25%), Positives = 193/476 (40%), Gaps = 58/476 (12%) Query: 2 MMNVLKRLSLVSMI---AGLTVITACGGNSASDNXXXXXXXXXXXXXXXXXPTQGKPVTI 58 M L R L+ ++ +G V+ ACG S G V I Sbjct: 1 MKQTLTRRELLRLMMAGSGAAVLAACGAQSGQSTGQSPTAPAVVSQ-------PGSTVKI 53 Query: 59 EFWYGLGGKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPAAVLS 118 +W G LGE +++++ FN SQ EV V+ Q Y ET QK AA+ P +L Sbjct: 54 TYWGSFSGNLGEAEQAMVKAFNDSQDEVEVEYQFQGSYEETAQKFTAALQANTTPDVILL 113 Query: 119 SNMDW---ARKGYFASMDELIAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQVMYY 175 S++ W G ++D+L A Q + + D+ L +G G Y++P +T + YY Sbjct: 114 SDVWWFGFYLAGAITALDDL-ARQVNLDFSDYEPVLLNEGVRKGVHYWIPFARSTPLFYY 172 Query: 176 RKDAFEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFGWEPMWGSGN-------MIDA 228 KD + ++G+ +TW + A A K+ DG + F G N + Sbjct: 173 NKDIWAEAGLPDRAPETWAEFAEWAPKLVKSDGSRAAF-------GHPNGASYIAWLFQG 225 Query: 229 VMSKGGSILSGDGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTID-DV 287 V+ + G S +T+ P + ++ + ++K W ++ D Sbjct: 226 VVWQFGGQYSQPDFTMTMTDPNTLRAAQFYQDTVLKEK----------WAILSPNLNQDF 275 Query: 288 MKGQAAGYTGSSGDQGDLDFSIVSAMEQPGWAGVGE-------GKPVAEALMAGIPAKAG 340 + G A S+G + + + GVG G P A +A I ++A Sbjct: 276 IGGAIASMMASTGALAGIQATATFPV------GVGFLPRETNFGCPTGGAGLA-IVSRAP 328 Query: 341 DAEKQAAMKWLTYFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIK-----VP 395 ++ AAMK++ + T+S N W+ +TGY+ VR S + P V F NP K +P Sbjct: 329 AEKQLAAMKYIAFATSSTNAGMWARSTGYMPVRISTKQTPEMVEFFNQNPNFKTAVDQLP 388 Query: 396 LMQASHASAPFQDPTGGKISDALKIAADKVQIENTPAAEALKEAQQTAQAALDKMK 451 +A A+ F I L+ + + AE E Q AQ L+ +K Sbjct: 389 KTRAQDAARVFVRNGDQIIGKGLERIIVNGETPSAVFAEVNNELTQAAQPILEDLK 444 >gb|AAO83404.1| putative SN-glycerol-3-phosphate-binding periplasmic protein [Enterobacter aerogenes] Length = 436 Score = 115 bits (288), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 100/356 (28%), Positives = 164/356 (46%), Gaps = 33/356 (9%) Query: 66 GKLGENMESLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPAAVLSS---NMD 122 GKL M +I+ FN SQQ+V V+GI +Y T+ K ++A GQ PA V+ S D Sbjct: 36 GKLTMEMTRVIKAFNESQQDVEVRGIFTGNYDTTKIKAESAQKAGQPPALVIMSANFTTD 95 Query: 123 WARKGYFASMDELIA----AQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQVMYYRKD 178 A K MDEL DF +++F K QV G Y +P + +T ++YY K Sbjct: 96 LALKDEILPMDELFKYGDQKAGDFLQKEFWPAMHKNAQVMGTTYAIPFHNSTPILYYNKT 155 Query: 179 AFEKSGIDASQLKTWEDLAAAAKKMAVQDGGKTTFFGWEPMWGSGN-------MIDAVMS 231 +++GI A +TW +L A AKK+ + G+ W M S N V + Sbjct: 156 LLDRAGI-AQPPQTWAELLADAKKLTDESKGQ-----WGIMLPSTNDDYGGWIFSALVRA 209 Query: 232 KGGSILSGDGT-KVTIDSPEWVETWDLFRKWIHEDKIMR---IHSGGQGWEYWYKTIDDV 287 GG + D +V +SP + ++ I++DK+M ++S ++ + Sbjct: 210 NGGKYFNEDYPGEVYYNSPTAIGALRFWQDLIYKDKVMPSGVLNSKQISASFFSGKLGMA 269 Query: 288 MKGQAA-GYTGSSGDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAEKQA 346 M A G+ + +L +++ A EQ P+ A + DA+K+A Sbjct: 270 MLSTGALGFMRENSKDFELGVAMLPAKEQ-------RAVPIGGASLVSFKG-INDAQKKA 321 Query: 347 AMKWLTYFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHA 402 A ++LTY + + WS TGY S R+++ P ++ + +P+ + L Q +A Sbjct: 322 AYQFLTYLVSPQVNGAWSRFTGYFSPRKASYDTPEMKAYLQQDPRAAIALEQLKYA 377 >ref|ZP_00036882.1| COG1653: ABC-type sugar transport system, periplasmic component [Enterococcus faecium] Length = 437 Score = 109 bits (272), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 104/424 (24%), Positives = 184/424 (43%), Gaps = 76/424 (17%) Query: 14 MIAGLTVITACGGNSASDNXXXXXXXXXXXXXXXXXPTQGKPVTIEFWYGLGGKLGENME 73 ++A ++ ACGG S++++ K VT+ FW G GE ++ Sbjct: 26 LVAATGILGACGGGSSAES------------------DSDKEVTLTFWNGFTASDGEILQ 67 Query: 74 SLIQKFNASQQEVIVKGIVQADYSETEQKLQAAIATGQVPAAVLSSNMDWARKGYFASMD 133 ++ +FN + + I + ++ +KL AI++ + P + + D FA Sbjct: 68 EIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD------FAQYV 121 Query: 134 ELIAAQP--DF------NKEDFVQTFLKQGQVDGKQYFLPMYGTTQVMYYRKDAFEKSGI 185 E A QP DF +K DF +T ++ G+V+GKQYF+PM MY+ D F+++G+ Sbjct: 122 ENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNNDLFKQAGL 181 Query: 186 DASQ-LKTWEDLAAAAKKMAVQDGGKTTF-FGWEPMWGSGNMIDAVMSKGGSILSGDGTK 243 D Q TWE+L A K+ + F F + G+ + + +++ GG +++ D TK Sbjct: 182 DTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKD---GTAPLYNWMLANGGKLVNDDYTK 238 Query: 244 VTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTIDDVMK-GQAA-----GYTG 297 SPE ++T +K IH DK G +D++M GQ A + Sbjct: 239 SEFASPENLKTLKAIQKMIHVDKTGPESISG-------AEMDNLMNAGQLAIEINGPWLN 291 Query: 298 SSGDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMAGIPAKAGDAEKQAAMKWLTYFTNS 357 + ++D+++ + P + + + + + IPA + +K+A ++L Y+ + Sbjct: 292 NGLKANEIDYNVTTL---PQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT 348 Query: 358 ENTAFWSINTGYISVRQSALKD-----------------------PAFVSFSETNPQIKV 394 E WSI G+ + S KD P F S N I Sbjct: 349 EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDIIN 408 Query: 395 PLMQ 398 PL++ Sbjct: 409 PLIE 412 >ref|NP_738126.1| putative glycerol-3-phosphate-binding periplasmic protein [Corynebacterium efficiens YS-314] dbj|BAC18326.1| putative glycerol-3-phosphate-binding periplasmic protein [Corynebacterium efficiens YS-314] Length = 441 Score = 108 bits (270), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 98/400 (24%), Positives = 167/400 (41%), Gaps = 61/400 (15%) Query: 20 VITACGGNSASDNXXXXXXXXXXXXXXXXXPTQGKPVTIEFWYGLGGKLGENMESLIQKF 79 +TAC G AS + +G TI +W G + +I +F Sbjct: 24 TLTACAGTGASTDTADATTA------------EGATNTIVWWSNHPGNSKDVELEIISRF 71 Query: 80 NASQQEVIVKGI-VQADYSETEQKLQAAIATGQVPAAVLSSNMDW---ARKGYFASMDEL 135 ++ V+ + A+Y+E QK AA++ G +P V+ S+ +W A G A++DE Sbjct: 72 EEENPDLTVQLVDAGANYAEVSQKFNAALSGGDLPDVVVLSDTEWFNFAVNGATANIDE- 130 Query: 136 IAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQVMYYRKDAFEKSGIDASQLKTWED 195 +A + + + +V + + DG Y LP +T + YY ++ + ++G+ ++W++ Sbjct: 131 VAKRNNIDTSSYVDSLYNDYEHDGGHYGLPFARSTTLFYYNRELWNEAGLPDRGPESWQE 190 Query: 196 LAAAAKKM-AVQDGGK-------TTFFGWE---PMWGSGNMIDAVMSKGGSILSGDGTKV 244 + K+ D GK + W PMW S GG+ G +++ Sbjct: 191 FSEWGPKIQEAMDSGKALGWGDAVNYLSWTFEGPMW----------SLGGNYSVGWESRL 240 Query: 245 TIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTIDDVMKGQAAGYTGSSGDQGD 304 T +PE + + + + + E W D+ A G GS Q Sbjct: 241 T--TPETIRSVEWLKSTVDEG--------------WATVSTDLTNEFATGIVGSC-IQST 283 Query: 305 LDFSIVSAMEQPGWA------GVGEGKPVAEALMAGIPAKAGDAEKQAAMKWLTYFTNSE 358 + V A WA GEG GIP+ + + A+K++ + TNS Sbjct: 284 GSLASVEAAATFDWAVAPLPNPTGEGACPTGGAGLGIPSGISEERQDNAIKFIDFLTNSA 343 Query: 359 NTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQ 398 NT +WS TGY+ VR+ A +DP +F + NP V + Q Sbjct: 344 NTGYWSRETGYVPVRKDAAEDPEHRAFLDENPAYNVAVEQ 383 >ref|NP_600604.1| ABC-type transporter, periplasmic component [Corynebacterium glutamicum ATCC 13032] dbj|BAB98778.1| ABC-type transporter, periplasmic component [Corynebacterium glutamicum ATCC 13032] emb|CAF21396.1| secreted sn-glycerol-3-phosphate-binding protein [Corynebacterium glutamicum ATCC 13032] Length = 438 Score = 107 bits (268), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 104/388 (26%), Positives = 166/388 (42%), Gaps = 61/388 (15%) Query: 75 LIQKFNASQQEVIVKGI-VQADYSETEQKLQAAIATGQVPAAVLSSNMDW---ARKGYFA 130 LI +F A ++ V+ + A+Y+E QK AA++ G +P V+ S+ +W A G Sbjct: 64 LISRFEAENPDLKVQLVDAGANYAEVSQKFNAALSGGDLPDLVVLSDTEWFNFAINGATV 123 Query: 131 SMDELIAAQPDFNKEDFVQTFLKQGQVDGKQYFLPMYGTTQVMYYRKDAFEKSGIDASQL 190 ++DE +A + + + +V + +G Y LP +T + YY KD + K+G++ Sbjct: 124 NIDE-VAKRNNIDTSTYVDSLFNDYSHEGGHYGLPFARSTVLFYYNKDLWAKAGLEDRGP 182 Query: 191 KTWEDLAAAAKKM--------AVQDGGKTTFFGWE---PMWGSGNMIDAVMSKGGSILSG 239 ++WE+ + K+ A G T + W PMW S GG+ G Sbjct: 183 ESWEEFSEWGPKLQEAMDSGFAHGWGDATNYLSWTFEGPMW----------SLGGNYSEG 232 Query: 240 DGTKVTIDSPEWVETWDLFRKWIHEDKIMRIHSGGQGWEYWYKTID-DVMKGQAAGYTGS 298 +++T +PE + + + + E + T+ DV A G GS Sbjct: 233 WESRLT--TPETIRAVEWLKSTVDEG---------------FATVSTDVTNEFATGLIGS 275 Query: 299 SGDQGDLDFSIVSAMEQPGWAGVGEGKPVAEALMA------GIPAKAGDAEKQAAMKWLT 352 Q D S V+ W P E GIP+ + + A+K++ Sbjct: 276 C-IQSTGDLSSVAGAASFDWGVAALPNPTGEGACPTGGAGLGIPSGISEQRQDNALKFID 334 Query: 353 YFTNSENTAFWSINTGYISVRQSALKDPAFVSFSETNPQIKVPLMQASHASAPFQD---- 408 + TN+ NT +WS TGY+ VR+ A DP +F E NP V + Q + QD Sbjct: 335 FLTNAANTGYWSRETGYVPVRKDAASDPDHAAFLEENPAYNVAVEQLPDTRS--QDNFRV 392 Query: 409 --PTGGK-ISDAL-KIAADKVQIENTPA 432 P G + I DAL KI I+ T A Sbjct: 393 LLPNGDRTIGDALEKICLTGADIDVTLA 420 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.129 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 647,955,422 Number of extensions: 26667203 Number of successful extensions: 54910 Number of sequences better than 1.0e-05: 225 Number of HSP's gapped: 54887 Number of HSP's successfully gapped: 226 Length of query: 451 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 322 Effective length of database: 406,989,807 Effective search space: 131050717854 Effective search space used: 131050717854 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 128 (53.9 bits)