BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0030.p (376 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pdb|1EL5|A Chain A, Complex Of Monomeric Sarcosine Oxidase With ... 290 4e-77 sp|P40859|MSOX_BACSP Monomeric sarcosine oxidase (MSOX) >gi|2126... 290 5e-77 prf||2105282A Sar oxidase 289 7e-77 pdb|1L9C|A Chain A, Role Of Histidine 269 In Catalysis By Monome... 288 2e-76 pir||T44248 sarcosine oxidase (EC 1.5.3.1) [imported] - Arthroba... 284 2e-75 >pdb|1EL5|A Chain A, Complex Of Monomeric Sarcosine Oxidase With The Inhibitor Dimethylglycine pdb|1EL5|B Chain B, Complex Of Monomeric Sarcosine Oxidase With The Inhibitor Dimethylglycine pdb|1EL7|A Chain A, Complex Of Monomeric Sarcosine Oxidase With The Inhibitor [methytelluro]acetate pdb|1EL7|B Chain B, Complex Of Monomeric Sarcosine Oxidase With The Inhibitor [methytelluro]acetate pdb|1EL8|A Chain A, Complex Of Monomeric Sarcosine Oxidase With The Inhibitor [methylseleno]cetate pdb|1EL8|B Chain B, Complex Of Monomeric Sarcosine Oxidase With The Inhibitor [methylseleno]cetate pdb|1L9F|A Chain A, Monomeric Sarcosine Oxidase: Structure Of A Covalently Flavinylated Amine Oxidizing Enzyme pdb|1L9F|B Chain B, Monomeric Sarcosine Oxidase: Structure Of A Covalently Flavinylated Amine Oxidizing Enzyme pdb|1L9E|A Chain A, Role Of Histidine 269 In Catalysis By Monomeric Sarcosine Oxidase pdb|1L9E|B Chain B, Role Of Histidine 269 In Catalysis By Monomeric Sarcosine Oxidase Length = 389 Score = 290 bits (742), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 157/367 (42%), Positives = 216/367 (58%), Gaps = 11/367 (2%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGY LA+QGVKTLLVDAFDPPHT GSHHG+TR+IRHAY Y+ LALR+ LW Sbjct: 14 MGMAAGYQLAKQGVKTLLVDAFDPPHTNGSHHGDTRIIRHAYGEGREYVPLALRSQELWY 73 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSF-ADRLAEAEKRQVRVERLDADEIRRRWPVLNLP 119 ELE+E+ ++ +GVL + +F A+ + A++ + V+ L+ DEI +RWP + +P Sbjct: 74 ELEKETHHKIFTKTGVLVFGPKGESAFVAETMEAAKEHSLTVDLLEGDEINKRWPGITVP 133 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 E++ A++EP++G L+SE CI YR+LA A GA+ SV + T +G Y Sbjct: 134 ENYNAIFEPNSGVLFSENCIRAYRELAEARGAKVLTHTRVEDFDISPDSVKIETANGSYT 193 Query: 180 GAAAILSAGAWFGSLPPFAAL--PIQAIRKVVGWFQS-----TPDFDAGSFPGFTLGTGE 232 I+S GAW L L P+Q R+VVG+F+S + D D FPGF + Sbjct: 194 ADKLIVSMGAWNSKLLSKLNLDIPLQPYRQVVGFFESDESKYSNDID---FPGFMVEVPN 250 Query: 233 GGYYGFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLL 292 G YYGFPS GG GLK+G H G+ P FG DE +LR LE Y+PGA G L Sbjct: 251 GIYYGFPSFGGCGLKLGYHTFGQKIDPDTINREFGVYPEDESNLRAFLEEYMPGANGELK 310 Query: 293 KGSVCKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNI 352 +G+VC Y T DE FIID HP + ++++A E+L+ LA ++ +I Sbjct: 311 RGAVCMYTKTLDEHFIIDLHPEHSNVVIAAGFSGHGFKFSSGVGEVLSQLALTGKTEHDI 370 Query: 353 GPFSLSR 359 FS++R Sbjct: 371 SIFSINR 377 >sp|P40859|MSOX_BACSP Monomeric sarcosine oxidase (MSOX) pir||I39975 sarcosine oxidase (EC 1.5.3.1) - Bacillus sp dbj|BAA03967.1| sarcosine oxidase [Bacillus sp.] Length = 390 Score = 290 bits (741), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 157/367 (42%), Positives = 216/367 (58%), Gaps = 11/367 (2%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGY LA+QGVKTLLVDAFDPPHT GSHHG+TR+IRHAY Y+ LALR+ LW Sbjct: 15 MGMAAGYQLAKQGVKTLLVDAFDPPHTNGSHHGDTRIIRHAYGEGREYVPLALRSQELWY 74 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSF-ADRLAEAEKRQVRVERLDADEIRRRWPVLNLP 119 ELE+E+ ++ +GVL + +F A+ + A++ + V+ L+ DEI +RWP + +P Sbjct: 75 ELEKETHHKIFTKTGVLVFGPKGESAFVAETMEAAKEHSLTVDLLEGDEINKRWPGITVP 134 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 E++ A++EP++G L+SE CI YR+LA A GA+ SV + T +G Y Sbjct: 135 ENYNAIFEPNSGVLFSENCIRAYRELAEARGAKVLTHTRVEDFDISPDSVKIETANGSYT 194 Query: 180 GAAAILSAGAWFGSLPPFAAL--PIQAIRKVVGWFQS-----TPDFDAGSFPGFTLGTGE 232 I+S GAW L L P+Q R+VVG+F+S + D D FPGF + Sbjct: 195 ADKLIVSMGAWNSKLLSKLNLDIPLQPYRQVVGFFESDESKYSNDID---FPGFMVEVPN 251 Query: 233 GGYYGFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLL 292 G YYGFPS GG GLK+G H G+ P FG DE +LR LE Y+PGA G L Sbjct: 252 GIYYGFPSFGGCGLKLGYHTFGQKIDPDTINREFGVYPEDESNLRAFLEEYMPGANGELK 311 Query: 293 KGSVCKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNI 352 +G+VC Y T DE FIID HP + ++++A E+L+ LA ++ +I Sbjct: 312 RGAVCMYTKTLDEHFIIDLHPEHSNVVIAAGFSGHGFKFSSGVGEVLSQLALTGKTEHDI 371 Query: 353 GPFSLSR 359 FS++R Sbjct: 372 SIFSINR 378 >prf||2105282A Sar oxidase Length = 390 Score = 289 bits (740), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 157/367 (42%), Positives = 215/367 (58%), Gaps = 11/367 (2%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGY LA+QGVKTLLVDAFDPPHT GSHHG+TR+IRHAY Y+ LALR+ LW Sbjct: 15 MGMAAGYQLAKQGVKTLLVDAFDPPHTNGSHHGDTRIIRHAYGEGREYVPLALRSQELWY 74 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSF-ADRLAEAEKRQVRVERLDADEIRRRWPVLNLP 119 ELE+E+ ++ +GVL + +F A+ + A++ + V L+ DEI +RWP + +P Sbjct: 75 ELEKETHHKIFTKTGVLVFGPKGESAFVAETMEAAKEHSLTVHLLEGDEINKRWPGITVP 134 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 E++ A++EP++G L+SE CI YR+LA A GA+ SV + T +G Y Sbjct: 135 ENYNAIFEPNSGVLFSENCIRAYRELAEARGAKVLTHTRVEDFDISPDSVKIETANGSYT 194 Query: 180 GAAAILSAGAWFGSLPPFAAL--PIQAIRKVVGWFQS-----TPDFDAGSFPGFTLGTGE 232 I+S GAW L L P+Q R+VVG+F+S + D D FPGF + Sbjct: 195 ADKLIVSMGAWNSKLLSKLNLDIPLQPYRQVVGFFESDESKYSNDID---FPGFMVEVPN 251 Query: 233 GGYYGFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLL 292 G YYGFPS GG GLK+G H G+ P FG DE +LR LE Y+PGA G L Sbjct: 252 GIYYGFPSFGGCGLKLGYHTFGQKIDPDTINREFGVYPEDESNLRAFLEEYMPGANGELK 311 Query: 293 KGSVCKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNI 352 +G+VC Y T DE FIID HP + ++++A E+L+ LA ++ +I Sbjct: 312 RGAVCMYTKTLDEHFIIDLHPEHSNVVIAAGFSGHGFKFSSGVGEVLSQLALTGKTEHDI 371 Query: 353 GPFSLSR 359 FS++R Sbjct: 372 SIFSINR 378 >pdb|1L9C|A Chain A, Role Of Histidine 269 In Catalysis By Monomeric Sarcosine Oxidase pdb|1L9C|B Chain B, Role Of Histidine 269 In Catalysis By Monomeric Sarcosine Oxidase pdb|1L9D|A Chain A, Role Of Histidine 269 In Catalysis By Monomeric Sarcosine Oxidase pdb|1L9D|B Chain B, Role Of Histidine 269 In Catalysis By Monomeric Sarcosine Oxidase Length = 389 Score = 288 bits (736), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 156/367 (42%), Positives = 216/367 (58%), Gaps = 11/367 (2%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGY LA+QGVKTLLVDAFDPPHT GSHHG+TR+IRHAY Y+ LALR+ LW Sbjct: 14 MGMAAGYQLAKQGVKTLLVDAFDPPHTNGSHHGDTRIIRHAYGEGREYVPLALRSQELWY 73 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSF-ADRLAEAEKRQVRVERLDADEIRRRWPVLNLP 119 ELE+E+ ++ +GVL + +F A+ + A++ + V+ L+ DEI +RWP + +P Sbjct: 74 ELEKETHHKIFTKTGVLVFGPKGESAFVAETMEAAKEHSLTVDLLEGDEINKRWPGITVP 133 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 E++ A++EP++G L+SE CI YR+LA A GA+ SV + T +G Y Sbjct: 134 ENYNAIFEPNSGVLFSENCIRAYRELAEARGAKVLTHTRVEDFDISPDSVKIETANGSYT 193 Query: 180 GAAAILSAGAWFGSLPPFAAL--PIQAIRKVVGWFQS-----TPDFDAGSFPGFTLGTGE 232 I+S GAW L L P+Q R+VVG+F+S + D D FPGF + Sbjct: 194 ADKLIVSMGAWNSKLLSKLNLDIPLQPYRQVVGFFESDESKYSNDID---FPGFMVEVPN 250 Query: 233 GGYYGFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLL 292 G YYGFPS GG GLK+G + G+ P FG DE +LR LE Y+PGA G L Sbjct: 251 GIYYGFPSFGGCGLKLGYNTFGQKIDPDTINREFGVYPEDESNLRAFLEEYMPGANGELK 310 Query: 293 KGSVCKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNI 352 +G+VC Y T DE FIID HP + ++++A E+L+ LA ++ +I Sbjct: 311 RGAVCMYTKTLDEHFIIDLHPEHSNVVIAAGFSGHGFKFSSGVGEVLSQLALTGKTEHDI 370 Query: 353 GPFSLSR 359 FS++R Sbjct: 371 SIFSINR 377 >pir||T44248 sarcosine oxidase (EC 1.5.3.1) [imported] - Arthrobacter sp. (strain TE1826) dbj|BAA09716.1| sarcosine oxidase [Arthrobacter sp.] dbj|BAA25926.1| sarcosine oxidase [Arthrobacter sp.] Length = 389 Score = 284 bits (727), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 148/364 (40%), Positives = 211/364 (57%), Gaps = 5/364 (1%) Query: 1 MGMSAGYYLARQGVKTLLVDAFDPPHTEGSHHGETRLIRHAYSGDPAYIDLALRAHVLWK 60 MGM+AGYYL++QGVKTLLVD+FDPPHT GSHHG+TR+IRHAY Y+ ALRA LW Sbjct: 17 MGMAAGYYLSKQGVKTLLVDSFDPPHTNGSHHGDTRIIRHAYGEGREYVPFALRAQELWY 76 Query: 61 ELEQESGTELLVPSGVLNLADQSVYSF-ADRLAEAEKRQVRVERLDADEIRRRWPVLNLP 119 ELE+E+ ++ +GVL + F A+ + A++ + V+ L+ EI +RWP + +P Sbjct: 77 ELEKETHHKIFTKTGVLVFGPKGEAPFVAETMEAAKEHSLDVDLLEGSEINKRWPGVTVP 136 Query: 120 EHFGAMYEPDAGYLYSEKCIAVYRKLALAHGAQXXXXXXXXXXXXXEGSVTVHTQSGDYH 179 E++ A++E ++G L+SE CI YR+LA A+GA+ E V + T G + Sbjct: 137 ENYNAIFEKNSGVLFSENCIRAYRELAEANGAKVLTYTPVEDFEIAEDFVKIQTAYGSFT 196 Query: 180 GAAAILSAGAWFGSL--PPFAALPIQAIRKVVGWFQSTPDFDAGS--FPGFTLGTGEGGY 235 + I+S GAW L +P+Q R+VVG+F+ + + +P F + G Y Sbjct: 197 ASKLIVSMGAWNSKLLSKLNIEIPLQPYRQVVGFFECDEKKYSNTHGYPAFMVEVPTGIY 256 Query: 236 YGFPSIGGAGLKIGRHDTGRVWRPGEPLAPFGSEESDEGDLRRVLEAYLPGAAGRLLKGS 295 YGFPS GG GLKIG H G+ P FG DEG++R+ LE Y+PGA G L G+ Sbjct: 257 YGFPSFGGCGLKIGYHTYGQKIDPDTINREFGIYPEDEGNIRKFLETYMPGATGELKSGA 316 Query: 296 VCKYEHTPDEDFIIDRHPAYRHILLAXXXXXXXXXXXXXXXEILADLAAGRASKQNIGPF 355 VC Y TPDE F+ID HP + ++ +A E L+ LA ++ +I F Sbjct: 317 VCMYTKTPDEHFVIDLHPQFSNVAIAAGFSGHGFKFSSVVGETLSQLAVTGKTEHDISIF 376 Query: 356 SLSR 359 S++R Sbjct: 377 SINR 380 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.139 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 573,642,381 Number of extensions: 24708482 Number of successful extensions: 52378 Number of sequences better than 1.0e-05: 104 Number of HSP's gapped: 52277 Number of HSP's successfully gapped: 105 Length of query: 376 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 249 Effective length of database: 412,420,005 Effective search space: 102692581245 Effective search space used: 102692581245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)