BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0028.p (447 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAJ13797.1| unknown [environmental sequence] 578 e-163 ref|NP_465866.1| similar to nitrilotriacetate monooxygenase [Lis... 563 e-159 ref|NP_471767.1| similar to nitrilotriacetate monooxygenase [Lis... 561 e-158 ref|ZP_00089347.1| COG2141: Coenzyme F420-dependent N5,N10-methy... 555 e-157 gb|EAJ13543.1| unknown [environmental sequence] 555 e-157 >gb|EAJ13797.1| unknown [environmental sequence] Length = 447 Score = 578 bits (1489), Expect = e-163, Method: Compositional matrix adjust. Identities = 271/436 (62%), Positives = 343/436 (78%) Query: 5 KPKQLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIADGLYI 64 + +++ LGA +HG GG + WRHPDA DAS+N + K ++AE K D +F+ADGL+I Sbjct: 12 RSRRIHLGATIHGPGGHYAGWRHPDAVIDASINPDFAKNVARQAEAAKFDFVFVADGLHI 71 Query: 65 NEKSIPHFLNRFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVISGGRAG 124 N KSIPHFLNRFEPLT+LSAL+ V+ ++GLVGTLSTSYSEPFTVARQF SLD +S GRAG Sbjct: 72 NAKSIPHFLNRFEPLTLLSALSAVTERVGLVGTLSTSYSEPFTVARQFASLDHLSRGRAG 131 Query: 125 WNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKETGVFFD 184 WNVVTSPLEGSA N+ R EHP+H +RYRIA EYL+V +GLWDSWE+DAFVR++ +GVFFD Sbjct: 132 WNVVTSPLEGSARNFSRTEHPEHDERYRIADEYLEVTKGLWDSWEEDAFVRDRASGVFFD 191 Query: 185 PEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFTGHDTLE 244 P K+HTLDH+G++FSV+GPLN+ R+ Q P++FQAG+SE GK A+ ADAVFT HDTL Sbjct: 192 PAKLHTLDHKGKYFSVEGPLNVGRTPQARPILFQAGASEAGKQLAATHADAVFTHHDTLA 251 Query: 245 EAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIENALNYL 304 +A+EFY D+K +V GR P ++ IF GIG I+G T E+AE++YQE L+TI +AL++L Sbjct: 252 QAREFYADLKQQVIEQGRQPADLKIFQGIGVIVGRTAEDAEQQYQESLKLITIGSALDHL 311 Query: 305 GRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQAATPRSS 364 GRFFEHHDF QY LDEPFPELG+LG NSF+SGTD+IK+NAR +GLTLR+VAL+AA PR Sbjct: 312 GRFFEHHDFGQYALDEPFPELGELGSNSFRSGTDEIKRNARARGLTLRQVALEAAAPRPR 371 Query: 365 FIGTPEQVADQIQAWFEXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGLFRTEYESDTL 424 FIGTPE +AD +Q WF+ P +FV++VVPIL+ERGLFR+EYE TL Sbjct: 372 FIGTPEHIADGLQEWFDAAAADGFIVGGGTPRAFTDFVEQVVPILRERGLFRSEYEGTTL 431 Query: 425 RGNLGLPVPANRYAKA 440 R +LG+ P+NRY+ A Sbjct: 432 RDHLGVRYPSNRYSAA 447 >ref|NP_465866.1| similar to nitrilotriacetate monooxygenase [Listeria monocytogenes EGD-e] pir||AG1367 nitrilotriacetate monooxygenase homolog lmo2343 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00421.1| lmo2343 [Listeria monocytogenes] Length = 440 Score = 563 bits (1451), Expect = e-159, Method: Compositional matrix adjust. Identities = 261/436 (59%), Positives = 334/436 (76%) Query: 3 MTKPKQLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIADGL 62 MT+ + ++ GA++HGVGG+T WRHPD P AS + + YK + AE+G +FIADGL Sbjct: 1 MTRKETIQFGAIIHGVGGTTDGWRHPDINPAASTDLDFYKTRAKIAEQGLFSFVFIADGL 60 Query: 63 YINEKSIPHFLNRFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVISGGR 122 +I+EKSIPHFLNRFEP+TILSALA + IGLVGT STS++EPFT+ARQ SLD IS GR Sbjct: 61 FISEKSIPHFLNRFEPITILSALAESTENIGLVGTFSTSFTEPFTLARQLSSLDHISSGR 120 Query: 123 AGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKETGVF 182 AGWN+VTSP EG+A N+ +K P H RY IAAE+L+V RGLW+SWE+DAF NKETG F Sbjct: 121 AGWNLVTSPQEGAARNHSKKNLPTHSDRYTIAAEHLKVVRGLWNSWEEDAFTYNKETGEF 180 Query: 183 FDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFTGHDT 242 F+PEK+H L+H+GE+F V+GPLNI RSKQG PV+FQAG+S G+++A++ A+A+FT D+ Sbjct: 181 FNPEKLHHLNHQGEYFQVEGPLNIGRSKQGEPVVFQAGASSTGRDFAAQNAEAIFTHSDS 240 Query: 243 LEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIENALN 302 LEEA EFYRDVK+R GRN DEV IFPGI PI+ DT EEAE KY E A L+ IENA+ Sbjct: 241 LEEAVEFYRDVKSRAEKAGRNADEVRIFPGISPIVADTLEEAEAKYTEFASLIPIENAVT 300 Query: 303 YLGRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQAATPR 362 YL R+F+ +D SQ+ LD PFP+LGD+G+N+FQS TD+IKK A+E+ LTLR+VA +AATP+ Sbjct: 301 YLARYFDDYDLSQFDLDAPFPDLGDIGKNAFQSTTDRIKKEAKERHLTLRQVATEAATPK 360 Query: 363 SSFIGTPEQVADQIQAWFEXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGLFRTEYESD 422 + FIGT EQVAD I+ WF+ +P E FV++V+P+LQ RGL+R EY + Sbjct: 361 TPFIGTKEQVADLIEVWFQNEAADGFIIASDIPGTFETFVEQVIPLLQSRGLYRNEYPAT 420 Query: 423 TLRGNLGLPVPANRYA 438 TLRGNLGL +P N+ A Sbjct: 421 TLRGNLGLTIPQNKQA 436 >ref|NP_471767.1| similar to nitrilotriacetate monooxygenase [Listeria innocua] pir||AH1736 nitrilotriacetate monooxygenase homolog lin2437 [imported] - Listeria innocua (strain Clip11262) emb|CAC97664.1| lin2437 [Listeria innocua] Length = 440 Score = 561 bits (1445), Expect = e-158, Method: Compositional matrix adjust. Identities = 259/436 (59%), Positives = 334/436 (76%) Query: 3 MTKPKQLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIADGL 62 MT+ ++ GA++HGVGG+T WRHPD P AS + + YK + AE+G +FIADGL Sbjct: 1 MTRKDTIQFGAIIHGVGGTTDGWRHPDINPAASTDLDFYKTRAKIAEQGLFSFVFIADGL 60 Query: 63 YINEKSIPHFLNRFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVISGGR 122 +I+EKSIPHFLNRFEP+TILSALA + IGLVGT STS++EPFT+ARQ SLD ISGGR Sbjct: 61 FISEKSIPHFLNRFEPITILSALAESTENIGLVGTFSTSFTEPFTLARQLSSLDHISGGR 120 Query: 123 AGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKETGVF 182 AGWN+VTSP EG+A N+ +K P H RY IAAE+L+V RGLW+SWE+DAF NKETG F Sbjct: 121 AGWNLVTSPQEGAARNHSKKNLPTHSDRYSIAAEHLKVVRGLWNSWEEDAFTYNKETGEF 180 Query: 183 FDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFTGHDT 242 F+PEK+H L+H+GE+F V+GPLNI RSKQG PV+FQAG+S G+++A++ A+A+FT D+ Sbjct: 181 FNPEKLHRLNHQGEYFQVEGPLNIGRSKQGEPVVFQAGASSTGRDFAAQNAEAIFTHSDS 240 Query: 243 LEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIENALN 302 LEEA +FY+DVK R GRN E+ IFPGI PI+ DT EEAE+KY E A L+ IENA+ Sbjct: 241 LEEAIQFYQDVKTRAEKAGRNAAEIRIFPGISPIVADTLEEAEQKYTEFANLIPIENAVT 300 Query: 303 YLGRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQAATPR 362 YL R+F+ +D SQ+ LDEPFP+LGD+G+N+FQS TD+IKK A+E+ LTLR+VA +AATP+ Sbjct: 301 YLARYFDDYDLSQFELDEPFPDLGDIGKNAFQSTTDRIKKEAKERNLTLRQVATEAATPK 360 Query: 363 SSFIGTPEQVADQIQAWFEXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGLFRTEYESD 422 + FIGT EQVAD I+ WF+ +P E FV++V+P+LQ RGL+R EY + Sbjct: 361 TPFIGTKEQVADLIEIWFQNEAADGFIIASDIPGTFETFVEQVIPLLQSRGLYRNEYPAT 420 Query: 423 TLRGNLGLPVPANRYA 438 TLRGNLGL +P N+ A Sbjct: 421 TLRGNLGLEIPKNKQA 436 >ref|ZP_00089347.1| COG2141: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases [Azotobacter vinelandii] Length = 500 Score = 555 bits (1431), Expect = e-157, Method: Compositional matrix adjust. Identities = 266/438 (60%), Positives = 331/438 (75%) Query: 1 MNMTKPKQLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIFIAD 60 + M P+QLKLGA++HGVG WRHPDA DASVNF Y+Q Q AE G+ D +FIAD Sbjct: 58 IAMGGPRQLKLGAIIHGVGHGWGDWRHPDAVADASVNFRFYRQQAQLAEAGRFDFLFIAD 117 Query: 61 GLYINEKSIPHFLNRFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDVISG 120 L+I+EKS PH+LNRFEPLTILSALA V+ IGLVGT + SY+EPF +ARQF SLD ISG Sbjct: 118 SLHIHEKSSPHYLNRFEPLTILSALATVTEHIGLVGTATVSYTEPFNLARQFASLDHISG 177 Query: 121 GRAGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNKETG 180 GRAGWNVVTS L G+A N+GR EH H RYRIA E+L V +GLWDSWEDDAFVR+K +G Sbjct: 178 GRAGWNVVTSWLSGTADNFGRPEHAPHDLRYRIAREHLNVVKGLWDSWEDDAFVRDKASG 237 Query: 181 VFFDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVFTGH 240 FFDP+++H L+H+GEFFSVKGPLNIARS QG PVIFQAGSSE G+N+A++ ADA+F Sbjct: 238 KFFDPDRLHALNHQGEFFSVKGPLNIARSPQGQPVIFQAGSSEEGRNFAAQNADAIFVNP 297 Query: 241 DTLEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTIENA 300 ++ +EA +YRD+K R A +GR+P ++ I PGI PI+G P E ER+Y++ LV+IE+A Sbjct: 298 ESFDEALAYYRDIKTRTAQYGRDPQKLSILPGIRPIVGRDPAEVERRYRQAVDLVSIEDA 357 Query: 301 LNYLGRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQAAT 360 L LGR F HDFS+YPLDEPFPELGD+GR+S Q ++ IK+ ARE+GL+LRE AL+ + Sbjct: 358 LVALGRPFNDHDFSRYPLDEPFPELGDIGRDSQQGESNHIKRVAREEGLSLREAALRFSR 417 Query: 361 PRSSFIGTPEQVADQIQAWFEXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGLFRTEYE 420 P +F+GTPEQ+AD +Q WFE +P+ L+ F + VVP+LQ RGLFR EY Sbjct: 418 PNRAFVGTPEQIADTLQHWFEKGAADGFTIGSLLPDSLQSFTELVVPVLQARGLFRREYA 477 Query: 421 SDTLRGNLGLPVPANRYA 438 TLR NLGL VP NRY+ Sbjct: 478 GHTLRDNLGLDVPVNRYS 495 >gb|EAJ13543.1| unknown [environmental sequence] Length = 444 Score = 555 bits (1430), Expect = e-157, Method: Compositional matrix adjust. Identities = 265/443 (59%), Positives = 337/443 (76%), Gaps = 5/443 (1%) Query: 3 MTKP-----KQLKLGALLHGVGGSTSMWRHPDAKPDASVNFELYKQWVQKAEEGKLDLIF 57 M++P + + LGA++ G G+ S WRHPDA DAS+N ++ ++AE K DL+F Sbjct: 1 MSQPTSASSRPIHLGAIIQGASGNMSAWRHPDAVADASINVTFVRELARQAEAAKFDLLF 60 Query: 58 IADGLYINEKSIPHFLNRFEPLTILSALAGVSSKIGLVGTLSTSYSEPFTVARQFGSLDV 117 +ADGL+IN KSIPHFLNRFEPLT+LSALA V+ +IGLVGTLSTSYS+PFTVARQF SLD Sbjct: 61 VADGLHINAKSIPHFLNRFEPLTLLSALALVTERIGLVGTLSTSYSDPFTVARQFSSLDH 120 Query: 118 ISGGRAGWNVVTSPLEGSALNYGRKEHPDHGKRYRIAAEYLQVARGLWDSWEDDAFVRNK 177 +S GRAGWNVVTSPLEGSA N+ R +HP+H RYRIA+E+L V +GLWDSWEDDAFVR+K Sbjct: 121 LSDGRAGWNVVTSPLEGSAKNFSRDKHPEHALRYRIASEFLTVTKGLWDSWEDDAFVRDK 180 Query: 178 ETGVFFDPEKMHTLDHEGEFFSVKGPLNIARSKQGGPVIFQAGSSEVGKNYASREADAVF 237 +GVFFDP+K+HTL+H GEFFSV GPLNI RS+QG P++FQAG+S+ GK A ADA++ Sbjct: 181 ASGVFFDPDKLHTLNHAGEFFSVAGPLNIGRSRQGRPILFQAGASDDGKGVAVEHADAIY 240 Query: 238 TGHDTLEEAQEFYRDVKARVASFGRNPDEVLIFPGIGPILGDTPEEAERKYQEVAGLVTI 297 T +T+ AQ FY DV+ R+A+ GR PD + +F G I+ + +AE +YQ+ A LVTI Sbjct: 241 TRQETIPLAQAFYADVQKRLAARGRAPDSLKVFQGTSVIVAASAADAEAQYQDTARLVTI 300 Query: 298 ENALNYLGRFFEHHDFSQYPLDEPFPELGDLGRNSFQSGTDKIKKNAREKGLTLREVALQ 357 +NAL+YLGR+F+HHDF+QY LD PFP+LG+LG N F+S TD IK++A E+GLTLREVAL+ Sbjct: 301 DNALDYLGRYFDHHDFTQYALDAPFPDLGELGGNQFRSTTDTIKRDAAERGLTLREVALE 360 Query: 358 AATPRSSFIGTPEQVADQIQAWFEXXXXXXXXXXXXVPNGLEEFVDRVVPILQERGLFRT 417 AATPR +F GTPEQVAD +QAW + P EF +VVPILQERG++R Sbjct: 361 AATPRPAFAGTPEQVADGLQAWVDARATDGFIVRGGTPTAFTEFATKVVPILQERGVYRR 420 Query: 418 EYESDTLRGNLGLPVPANRYAKA 440 +YE TLR +LGL P NR+A+A Sbjct: 421 DYEGATLREHLGLAYPVNRHAQA 443 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 692,227,804 Number of extensions: 29758835 Number of successful extensions: 73177 Number of sequences better than 1.0e-05: 287 Number of HSP's gapped: 72636 Number of HSP's successfully gapped: 290 Length of query: 447 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 318 Effective length of database: 406,989,807 Effective search space: 129422758626 Effective search space used: 129422758626 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)