BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0016.p (375 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00036973.1| hypothetical protein [Enterococcus faecium] 233 5e-60 ref|NP_242920.1| unsaturated glucuronyl hydrolase [Bacillus halo... 232 1e-59 ref|ZP_00036971.1| hypothetical protein [Enterococcus faecium] 220 5e-56 ref|NP_811825.1| unsaturated glucuronylhydrolase [Bacteroides th... 214 2e-54 ref|NP_814555.1| glucuronyl hydrolase, putative [Enterococcus fa... 213 6e-54 >ref|ZP_00036973.1| hypothetical protein [Enterococcus faecium] Length = 372 Score = 233 bits (594), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 131/374 (35%), Positives = 197/374 (52%), Gaps = 14/374 (3%) Query: 6 WVEEAWSKALEKTGINSQSIGAEFPHASQGGKY--VLE--APDWWTAGFWPGMLWQFYGE 61 W + + A EK +G + P+ S GKY V++ DWWT GFW G+LWQ + + Sbjct: 8 WSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVIKDRGIDWWTNGFWSGILWQLFHK 67 Query: 62 CGDESLKAIAEHCEKRLDEVLDGYVRLDHDLGFMWTLTSVANYKLTGNEISRIRALKAAN 121 E K A E+ L+ L + + HD+GF++ +++A+Y+ + N+ SR L AA Sbjct: 68 TKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLFLPSAIAHYQQSQNKHSRQLGLHAAT 127 Query: 122 YLSARFNLKGRYIRAWNPWREGEDNSGVAIIDCCMNTSLLFWASAETGDPRYRHIAEAHM 181 L+ R+N+ G Y+ AWN + G IID MN LL+WAS ETGDPR+ IA H Sbjct: 128 VLAGRYNISGNYLVAWN-----DHKPGWMIIDSMMNLPLLYWASRETGDPRFSKIARQHA 182 Query: 182 DTVLEYFIRADGSVYHIVNFNPDTGEVKEKLGGQGYGPESAWSRGAAWAIYGLTLAYHHS 241 DT +Y +R DGS HI F+ ++G+ LGGQGY S WSRG AWA+YG L+Y ++ Sbjct: 183 DTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGYNENSCWSRGNAWALYGFALSYRYT 242 Query: 242 GKQEYLHAAQRVADFFLTRLPED-HVPHWDFRAPGEIGEIRDTXXXXXXXXXXXXXXXXX 300 YL A+++A F+ ++ + ++P DF DT Sbjct: 243 KNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK---DSNIDTSAGVCAACGLLELAGYV 299 Query: 301 XDPESHMYRSGALKIMESLFRNYGTWDQPDEEGLLLHGTSNYPENRNIDVPLIYGDFFYV 360 S MY A+ I++ ++Y W+ DE+G++ G Y +VPLIY D+F++ Sbjct: 300 SSVRSKMYHEAAINILQKTEKHYANWNM-DEDGIIGGGAEAYHRELTYNVPLIYSDYFFI 358 Query: 361 EALARLKEAGPFYW 374 E L RL+++ W Sbjct: 359 EGLLRLEQSELAIW 372 >ref|NP_242920.1| unsaturated glucuronyl hydrolase [Bacillus halodurans] pir||F83906 unsaturated glucuronyl hydrolase BH2054 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05773.1| unsaturated glucuronyl hydrolase [Bacillus halodurans] Length = 375 Score = 232 bits (591), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 133/372 (35%), Positives = 193/372 (51%), Gaps = 9/372 (2%) Query: 8 EEAWSKALEKTGINSQSIGAEFPHASQGGK-YVLEAPDWWTAGFWPGMLWQFYGECGDES 66 ++A + EKT N + FPH S+ G+ Y L + WT GFW G+LW Y D + Sbjct: 4 KQAMTDVAEKTLTNIKRFNGRFPHVSEDGEHYELNNNNEWTNGFWSGILWLCYEYTNDPA 63 Query: 67 LKAIAEHCEKRLDEVLDGYVRLDH-DLGFMWTLTSVANYKLTGNEISRIRALKAANYLSA 125 + A + + ++ + LDH D+GF+++L+S A + + +E ++ ++AA+ L Sbjct: 64 FRQAAASTVRSFQQRMEQNLELDHHDIGFLYSLSSKAQWIIERDERAKQLTIEAADVLMK 123 Query: 126 RFNLKGRYIRAWNPWREGE-DNSGVAIIDCCMNTSLLFWASAETGDPRYRHIAEAHMDTV 184 R+ K +AW P EG+ N G I+DC MN LLFWAS TG+P YR A H D Sbjct: 124 RWREKIELFQAWGP--EGDLSNGGRIIVDCLMNLPLLFWASEVTGNPDYREAAIIHADKT 181 Query: 185 LEYFIRADGSVYHIVNFNPDTGEVKEKLGGQGYGPESAWSRGAAWAIYGLTLAYHHSGKQ 244 + +R D S YH FN +TGE QGY S WSRG AWAIYG +AY ++G + Sbjct: 182 RRFIVRGDDSTYHTFYFNQETGEALRGGTHQGYEDGSTWSRGQAWAIYGFAIAYRYTGNE 241 Query: 245 EYLHAAQRVADFFLTRLPEDHVPHWDFRAPGEIGEIRDTXXXXXXX--XXXXXXXXXXXD 302 YL A+R A +F+ LP D+V +WDF AP RD+ D Sbjct: 242 RYLETAKRTAKYFIENLPADYVAYWDFNAPITPDTKRDSSASAIASCGILELLSHLQETD 301 Query: 303 PESHMYRSGALKIMESLFRNYGTWDQPDEEGLLLHGTSNYPENRNIDVPLIYGDFFYVEA 362 P+ ++ K M SL NY + + D +GL+ G+ + D +I+GD+FY EA Sbjct: 302 PDKAFFQQSVQKQMTSLVENYAS--EKDAQGLIKRGSYSVRIGHAPDDYVIWGDYFYTEA 359 Query: 363 LARLKEAGPFYW 374 L RL++ YW Sbjct: 360 LMRLEKLRNGYW 371 >ref|ZP_00036971.1| hypothetical protein [Enterococcus faecium] Length = 407 Score = 220 bits (560), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 124/380 (32%), Positives = 194/380 (51%), Gaps = 16/380 (4%) Query: 7 VEEAWSKALEKTGINSQSI-------GAEFP-HASQGGKYVLEAPDWWTAGFWPGMLWQF 58 +E+ W+ +E+ +++ + +FP G+Y L WT GFW G+ W Sbjct: 32 MEKEWNNEIERCRRHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLL 91 Query: 59 YGECGDESLKAIAEHCEKRLDEVLDGYVRLDH-DLGFMWTLTSVANYKLTGNEISRIRAL 117 + + GD + AE + +D + +H DLGF+++L++VA+YK+TGN + ++ Sbjct: 92 FEKTGDPLFQQTAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASI 151 Query: 118 KAANYLSARFNLKGRYIRAW-NPWREGEDNSGVAIIDCCMNTSLLFWASAETGDPRYRHI 176 AA L RF K I+AW +P G I+DCCMN LL+WAS ETG+ +Y+ Sbjct: 152 DAAETLLQRFWEKAGVIQAWGDP--SDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKA 209 Query: 177 AEAHMDTVLEYFIRADGSVYHIVNFNPDTGEVKEKLGGQGYGPESAWSRGAAWAIYGLTL 236 A+AH+ +Y +R DGS YH + G+ + QGY S W+RG AW IYG L Sbjct: 210 AQAHIQNAADYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFAL 269 Query: 237 AYHHSGKQEYLHAAQRVADFFLTRLPEDHVPHWDFRAPGEIGEIRDTXXXXXXXX--XXX 294 +Y + +Q+Y ++ +A++F+ RLPED + +WD E +DT Sbjct: 270 SYRYLKEQKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLEL 329 Query: 295 XXXXXXXDPESHMYRSGALKIMESLFRNYGTWDQPDEEGLLLHGTSNYPENRNIDVPLIY 354 E ++Y+S KIM L Y T +E+G L N P+N ++ P I+ Sbjct: 330 IESGLLDKTEKNLYQSAVWKIMRQLKERYAT--TEEEDGFLTQSVYNKPKNNGVNTPCIW 387 Query: 355 GDFFYVEALARLKEAGPFYW 374 GD++Y+EAL R+ YW Sbjct: 388 GDYYYLEALVRMTSNWSSYW 407 >ref|NP_811825.1| unsaturated glucuronylhydrolase [Bacteroides thetaiotaomicron VPI-5482] gb|AAO78019.1| unsaturated glucuronylhydrolase [Bacteroides thetaiotaomicron VPI-5482] Length = 402 Score = 214 bits (546), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 123/346 (35%), Positives = 183/346 (52%), Gaps = 25/346 (7%) Query: 34 QGGKYVLEAPDWWTAGFWPGMLWQFYGECGDESLKAIAEHCEKRLDEVLDGYVRLDHDLG 93 + GK + +WWT+GF+PG+LW Y G++SLK AE+ KR++ Y +HD+G Sbjct: 61 KDGKLMTSKSNWWTSGFFPGVLWYLYEVNGNDSLKMYAENYTKRIEN--QKYTTDNHDVG 118 Query: 94 FMWTLTSVANYKLTGNEISRIRALKAANYLSARFNLKGRYIRAWNPWREGEDNSGVAIID 153 FM + +LTGN+ R L+ + LS RF + IR+W+ W + V IID Sbjct: 119 FMLYCSFGNGLRLTGNDDYRKTLLQGSESLSTRFRPQVGCIRSWD-WNQKVWEYPV-IID 176 Query: 154 CCMNTSLLFWASAETGDPRYRHIAEAHMDTVLEYFIRADGSVYHIVNFNPDTGEVKEKLG 213 MN +L WAS + DP++ IA++H D ++Y R+D S YH+++++ +G ++K Sbjct: 177 NLMNLEMLMWASKNSDDPKFEEIAKSHADVTMKYHFRSDYSSYHVISYDTISGLPEKKNT 236 Query: 214 GQGYGPESAWSRGAAWAIYGLTLAYHHSGKQEYLHAAQRVADFFLT--RLPEDHVPHWDF 271 QGY ES W+RG WA+YG T+ Y +G+++YL A VA F + RLPED +P+WDF Sbjct: 237 CQGYAHESCWARGQGWALYGYTMMYRETGQEKYLRHAINVAKFIINHPRLPEDKIPYWDF 296 Query: 272 RAPGEIGEIRDTXXXXXXXXXXXXXXXXXXDPESHMYRSG-----ALKIMESLFRNYGTW 326 AP E+RD E +Y G L + E+ + + Sbjct: 297 DAPNIPNELRDASAGALMASAFI---------ELSLYTEGDFSKQCLSVAETQLKTLSSP 347 Query: 327 DQPDEEG-----LLLHGTSNYPENRNIDVPLIYGDFFYVEALARLK 367 + E G +L H N P +DVPL Y D++YVEAL R K Sbjct: 348 EYLAEPGTNCNFILKHSVGNNPGKAEVDVPLTYADYYYVEALVRYK 393 >ref|NP_814555.1| glucuronyl hydrolase, putative [Enterococcus faecalis V583] gb|AAO80625.1| glucuronyl hydrolase, putative [Enterococcus faecalis V583] Length = 387 Score = 213 bits (542), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 125/372 (33%), Positives = 192/372 (51%), Gaps = 8/372 (2%) Query: 6 WVEEAWSKALEKTGINSQSIGAEFPHA-SQGGKYVLEAPDWWTAGFWPGMLWQFYGECGD 64 W++ ++ + K N Q G +FP A + G+Y L+A D WT GFW GMLW Y Sbjct: 21 WIQTQLNECVSKIAKNMQRFGTQFPSACATNGRYRLKANDDWTNGFWTGMLWLAYEWSHQ 80 Query: 65 ESLKAIAEHCEKRLDEVLDGYVRLDH-DLGFMWTLTSVANYKLTGNEISRIRALKAANYL 123 E + +A + L+ + LDH D+GF+++L++ A YK+TG+E + L AA L Sbjct: 81 EKFRLLAMENIDSFQQRLEEHFVLDHHDIGFLYSLSAGAGYKITGDERCKQEVLAAAEVL 140 Query: 124 SARFNLKGRYIRAWNPWREGEDNSGVAIIDCCMNTSLLFWASAETGDPRYRHIAEAHMDT 183 SARF G +I+AW + G+ IID MN LLF A+ +G+ Y IA H T Sbjct: 141 SARFQENGSFIQAWGHY--GDPKEYRLIIDSLMNLPLLFEATKLSGNRVYEEIASRHYHT 198 Query: 184 VLEYFIRADGSVYHIVNFNPDTGEVKEKLGGQGYGPESAWSRGAAWAIYGLTLAYHHSGK 243 +++ I++D + YH F+P+TG+ K QG+ S W+RG +WAI G+ L Sbjct: 199 LMKTVIKSDATTYHTYYFDPETGKPKHGATHQGHSDTSIWARGQSWAILGIPLNETFLHS 258 Query: 244 QEYLHAAQRVADFFLTRLPEDHVPHWDFRAPGEIGEIRDTXXXXXXXXXXXXXXXXXXDP 303 Q + ++ + FL LP D +P+WDF ++ +D+ P Sbjct: 259 QPFPEMYPQIVEVFLAHLPADLIPYWDFDFNDQMPSDKDSSALAIAACGLLEADKLQAFP 318 Query: 304 ESHMYRSGALKIMESLFRNYGTWDQPDEEGLLLHGTSNYPENRNIDVPLIYGDFFYVEAL 363 ++ G + + +R T + + EGLLLHG + E + ID P ++GD+FY+EAL Sbjct: 319 QAKELAKGMIYQLGEYYR---TQNDSENEGLLLHGVYAHAEGKGIDEPNLWGDYFYMEAL 375 Query: 364 ARL-KEAGPFYW 374 RL K + YW Sbjct: 376 MRLAKPSWQRYW 387 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 600,783,686 Number of extensions: 24993805 Number of successful extensions: 50603 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 50507 Number of HSP's successfully gapped: 27 Length of query: 375 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 248 Effective length of database: 412,420,005 Effective search space: 102280161240 Effective search space used: 102280161240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)