BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0470.0014.p (255 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_632209.1| Short chain dehydrogenase/reductase [Methanosar... 206 5e-52 ref|ZP_00061090.1| COG1028: Dehydrogenases with different specif... 202 7e-51 ref|NP_615081.1| gluconate 5-dehydrogenase [Methanosarcina aceti... 200 2e-50 ref|NP_406070.1| gluconate 5-dehydrogenase [Yersinia pestis] >gi... 168 1e-40 ref|NP_993678.1| gluconate 5-dehydrogenase [Yersinia pestis biov... 168 1e-40 >ref|NP_632209.1| Short chain dehydrogenase/reductase [Methanosarcina mazei Goe1] gb|AAM29881.1| Short chain dehydrogenase/reductase [Methanosarcina mazei Goe1] Length = 255 Score = 206 bits (523), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 102/255 (40%), Positives = 150/255 (58%) Query: 1 MNNLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQT 60 M NLFDL+G+ E A+A A QGAD+A++ R +KL +EIE G Sbjct: 1 MKNLFDLTGRVAIVTGASSGLGVEFARALANQGADIAIVARREEKLKEVQKEIEKIGVTC 60 Query: 61 LSLGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKS 120 CDV++ D+I++AV+ I + +G+IDILVNNAG+ + ++ + W +IDTNL Sbjct: 61 RYYICDVMQTDQIKSAVEKIEKDFGKIDILVNNAGLGLVDAADKTSDEMWRNMIDTNLNG 120 Query: 121 IFLMSKHVIKPMKERRYGRIINMASICGMTGSKSFPLHAYNASKGAVLSITRGMGASLAP 180 +F ++ V K M +++YGRIIN+ SI K P+ AY ++KG VL +T+ + A Sbjct: 121 VFFFAREVGKVMLKQKYGRIINIGSIHSTVSMKGMPVTAYCSTKGGVLMLTKSLATEWAK 180 Query: 181 YGITVNAIGPSLFRTEMTERALYQDSFLQLYNAQCPIGRPGNPEEINGAAIYFASDASSY 240 GITVNAIGP F M E + F ++ CP+GR G +++ +Y AS+AS Y Sbjct: 181 EGITVNAIGPGYFALGMAEGVVADPEFAKIIEFMCPMGRAGQTGDLDTTVVYLASEASKY 240 Query: 241 TTGQTLYVDGGWTSV 255 TGQ + VDGGWT++ Sbjct: 241 ITGQIITVDGGWTAL 255 >ref|ZP_00061090.1| COG1028: Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) [Clostridium thermocellum ATCC 27405] Length = 260 Score = 202 bits (513), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 102/253 (40%), Positives = 149/253 (58%) Query: 3 NLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQTLS 62 +LF L G+ ++AKA AAQGAD+ +L R +KL A EI G + L Sbjct: 8 DLFSLKGRVAVVTGASSGLGVQMAKALAAQGADIVILARRKEKLEKVAEEIRQFGVRCLP 67 Query: 63 LGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKSIF 122 + CDV + + + A + + +G++DIL+NNAG EE + W I+ +L +F Sbjct: 68 IECDVTKIEMVRKAAELAEKEFGKVDILINNAGSGGIAPAEETSDEMWSNTINVDLSGVF 127 Query: 123 LMSKHVIKPMKERRYGRIINMASICGMTGSKSFPLHAYNASKGAVLSITRGMGASLAPYG 182 ++++ K M + +YGRIIN++SI GM G+ + P AY+A+KG V+++TR + A A YG Sbjct: 128 MVAREFGKIMIKNKYGRIINISSIYGMVGNMAHPSAAYHAAKGGVINLTRALAAEWAKYG 187 Query: 183 ITVNAIGPSLFRTEMTERALYQDSFLQLYNAQCPIGRPGNPEEINGAAIYFASDASSYTT 242 ITVNAI P F TE+TE L + F + P+GR G E++ A+Y ASDASSY T Sbjct: 188 ITVNAICPGYFVTELTEDVLNTEYFTNYMKSTVPMGRYGKEGELDSTAVYLASDASSYVT 247 Query: 243 GQTLYVDGGWTSV 255 G + VDGG+T V Sbjct: 248 GAVIPVDGGYTCV 260 >ref|NP_615081.1| gluconate 5-dehydrogenase [Methanosarcina acetivorans str. C2A] gb|AAM03561.1| gluconate 5-dehydrogenase [Methanosarcina acetivorans str. C2A] Length = 255 Score = 200 bits (509), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 98/255 (38%), Positives = 148/255 (58%) Query: 1 MNNLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQT 60 M NLFDL+GK E A+A A QGA++A++ R +KL +EIE G Sbjct: 1 MKNLFDLTGKVAIVTGASSGLGVEFARALANQGANIAIIARREEKLKEVQKEIEKLGVTC 60 Query: 61 LSLGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKS 120 CDV++ ++I+ AV+ +++ +G+IDILVNNAG+ + ++ + W +IDTNL Sbjct: 61 RYYLCDVMKTEQIKNAVEQVVKDFGRIDILVNNAGLGLVDAADKTTDEMWHNMIDTNLNG 120 Query: 121 IFLMSKHVIKPMKERRYGRIINMASICGMTGSKSFPLHAYNASKGAVLSITRGMGASLAP 180 ++ ++ V K M +++GRIIN+ SI K P+ AY ++KG VL +T+ + A Sbjct: 121 VYFFAREVGKIMLRQKHGRIINIGSIHSTVSMKGLPVTAYCSTKGGVLMLTKSLATEWAK 180 Query: 181 YGITVNAIGPSLFRTEMTERALYQDSFLQLYNAQCPIGRPGNPEEINGAAIYFASDASSY 240 GITVNAIGP F M E + F ++ CP+GR G +++ +Y ASD S Y Sbjct: 181 EGITVNAIGPGYFALGMAEGVVADPEFAKIIEFMCPMGRAGQSGDLDTTVVYLASDESKY 240 Query: 241 TTGQTLYVDGGWTSV 255 TGQ + VDGGWT++ Sbjct: 241 ITGQIITVDGGWTAL 255 >ref|NP_406070.1| gluconate 5-dehydrogenase [Yersinia pestis] pir||AI0309 gluconate 5-dehydrogenase (EC 1.1.1.69) [imported] - Yersinia pestis (strain CO92) emb|CAC91341.1| gluconate 5-dehydrogenase [Yersinia pestis CO92] Length = 254 Score = 168 bits (425), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 92/251 (36%), Positives = 137/251 (54%), Gaps = 2/251 (0%) Query: 1 MNNLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQT 60 M NLF L + LAK A GA++ + D+ ++ E+ ++G Sbjct: 1 MKNLFSLENRKVLITGSAQGIGFLLAKGLAEFGAEIIINDITAERAEKAVAELRASGFIA 60 Query: 61 LSLGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKS 120 + +V DE+ A++ I H G ID+L+NNAG+ + E E++WD VI N KS Sbjct: 61 HAAAFNVTNHDEVNEAIEKIESHIGAIDVLINNAGIQRRHAFTEFPEKDWDDVIAVNQKS 120 Query: 121 IFLMSKHVIKPMKERRYGRIINMASICGMTGSKSFPLHAYNASKGAVLSITRGMGASLAP 180 +FL+S+ V + M +R+ G+IIN+ S+ G + + Y ASKGAV +TRGM LA Sbjct: 121 VFLVSQAVARYMVKRQRGKIINICSMQSELGRDT--ITPYAASKGAVKMLTRGMCVELAR 178 Query: 181 YGITVNAIGPSLFRTEMTERALYQDSFLQLYNAQCPIGRPGNPEEINGAAIYFASDASSY 240 Y I VN I P F+T+MT+ + +F + P R G+PEE+ GAA+Y +S AS + Sbjct: 179 YNIQVNGIAPGYFKTDMTKALVDDQAFTDWLCKRTPAARWGDPEELIGAAVYLSSKASDF 238 Query: 241 TTGQTLYVDGG 251 G L+VDGG Sbjct: 239 VNGHLLFVDGG 249 >ref|NP_993678.1| gluconate 5-dehydrogenase [Yersinia pestis biovar Mediaevails str. 91001] gb|AAS62555.1| gluconate 5-dehydrogenase [Yersinia pestis biovar Mediaevails str. 91001] Length = 289 Score = 168 bits (425), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 92/251 (36%), Positives = 137/251 (54%), Gaps = 2/251 (0%) Query: 1 MNNLFDLSGKXXXXXXXXXXXXXELAKAFAAQGADVALLDLRPDKLITPAREIESAGRQT 60 M NLF L + LAK A GA++ + D+ ++ E+ ++G Sbjct: 36 MKNLFSLENRKVLITGSAQGIGFLLAKGLAEFGAEIIINDITAERAEKAVAELRASGFIA 95 Query: 61 LSLGCDVIREDEIETAVQAILQHWGQIDILVNNAGVASAGSVEELEEREWDRVIDTNLKS 120 + +V DE+ A++ I H G ID+L+NNAG+ + E E++WD VI N KS Sbjct: 96 HAAAFNVTNHDEVNEAIEKIESHIGAIDVLINNAGIQRRHAFTEFPEKDWDDVIAVNQKS 155 Query: 121 IFLMSKHVIKPMKERRYGRIINMASICGMTGSKSFPLHAYNASKGAVLSITRGMGASLAP 180 +FL+S+ V + M +R+ G+IIN+ S+ G + + Y ASKGAV +TRGM LA Sbjct: 156 VFLVSQAVARYMVKRQRGKIINICSMQSELGRDT--ITPYAASKGAVKMLTRGMCVELAR 213 Query: 181 YGITVNAIGPSLFRTEMTERALYQDSFLQLYNAQCPIGRPGNPEEINGAAIYFASDASSY 240 Y I VN I P F+T+MT+ + +F + P R G+PEE+ GAA+Y +S AS + Sbjct: 214 YNIQVNGIAPGYFKTDMTKALVDDQAFTDWLCKRTPAARWGDPEELIGAAVYLSSKASDF 273 Query: 241 TTGQTLYVDGG 251 G L+VDGG Sbjct: 274 VNGHLLFVDGG 284 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 337,216,539 Number of extensions: 12866290 Number of successful extensions: 64121 Number of sequences better than 1.0e-05: 8335 Number of HSP's gapped: 54294 Number of HSP's successfully gapped: 8355 Length of query: 255 Length of database: 757,237,578 Length adjustment: 123 Effective length of query: 132 Effective length of database: 423,280,401 Effective search space: 55873012932 Effective search space used: 55873012932 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)