BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0035.p (180 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_782402.1| putative phosphoesterase [Clostridium tetani E8... 204 8e-52 ref|NP_349355.1| Predicted phosphoesterase [Clostridium acetobut... 200 1e-50 ref|ZP_00129022.1| COG0622: Predicted phosphoesterase [Desulfovi... 187 8e-47 dbj|BAA77455.1| orf2dG [Clostridium histolyticum] 187 1e-46 ref|NP_797195.1| conserved hypothetical protein [Vibrio parahaem... 178 6e-44 >ref|NP_782402.1| putative phosphoesterase [Clostridium tetani E88] gb|AAO36339.1| putative phosphoesterase [Clostridium tetani E88] Length = 181 Score = 204 bits (519), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 94/180 (52%), Positives = 124/180 (68%) Query: 1 MKLMFISDIHGSLYWXXXXXXXXXXXXPHTLVVLGDFLYHGPRNPLPDGYDPQGVAARLN 60 MKL FISDIHGS Y+ LV+LGD LYHG RNPLP Y+P+ VA LN Sbjct: 1 MKLFFISDIHGSSYYLEKVINIYEKEKADYLVILGDELYHGARNPLPKEYNPKKVAEILN 60 Query: 61 AYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHEGRKIYATHGHGFSIDNLPPLAPGD 120 Y ++AVRGNCD+EVDQM+L +P+M DY +IL+ R+++ THGH ++ +NLP ++ GD Sbjct: 61 KYKNKIIAVRGNCDSEVDQMVLNYPIMSDYSIILYNNRRLFLTHGHIYNKNNLPTISNGD 120 Query: 121 VFIQGHTHLPVADVKEGITVLNPGSISLPKENNPNSYAILENGEFIIKDFAGKIVKQIRL 180 VFI GHTH+P+A I ++NPGSI+ PKEN P+ Y ILEN FIIK G++ K+I + Sbjct: 121 VFIYGHTHVPLAKRINNIFIINPGSITFPKENTPHCYGILENNTFIIKTLDGEVFKEINI 180 >ref|NP_349355.1| Predicted phosphoesterase [Clostridium acetobutylicum] pir||D97238 probable phosphoesterase [imported] - Clostridium acetobutylicum gb|AAK80695.1| Predicted phosphoesterase [Clostridium acetobutylicum] Length = 180 Score = 200 bits (508), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 90/180 (50%), Positives = 128/180 (71%) Query: 1 MKLMFISDIHGSLYWXXXXXXXXXXXXPHTLVVLGDFLYHGPRNPLPDGYDPQGVAARLN 60 MK+ F+SDIHGSL++ +V+LGD LYHG RNPLP GY+P+ V+ LN Sbjct: 1 MKIFFMSDIHGSLHYLEKALARFKEENADFIVILGDELYHGARNPLPLGYNPKAVSELLN 60 Query: 61 AYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHEGRKIYATHGHGFSIDNLPPLAPGD 120 +Y + ++A+RGNCD+EVD+M+L +P+M Y IL++ ++++ THGH + +NLP L GD Sbjct: 61 SYSEQIIAIRGNCDSEVDEMVLTYPIMSTYSTILYKDKRLFLTHGHVYGEENLPRLRRGD 120 Query: 121 VFIQGHTHLPVADVKEGITVLNPGSISLPKENNPNSYAILENGEFIIKDFAGKIVKQIRL 180 VF+ GHTH+PVA K+ I ++NPGSIS+PKEN+PNSYA+ EN F IKD AG ++ + Sbjct: 121 VFLYGHTHVPVAKKKDDIYIINPGSISIPKENSPNSYAVFENDLFEIKDLAGNTFMKLNI 180 >ref|ZP_00129022.1| COG0622: Predicted phosphoesterase [Desulfovibrio desulfuricans G20] Length = 186 Score = 187 bits (476), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 92/180 (51%), Positives = 120/180 (66%), Gaps = 5/180 (2%) Query: 1 MKLMFISDIHGSLYWXXXXXXXXXXXXPHTLVVLGDFLYHGPRNPLPDGYDPQGVAARLN 60 MK++F+SDIHGS P + +LGD LYHGPRNPLP YDP+GVAA LN Sbjct: 1 MKILFVSDIHGSAGAARTVLEKAEAMQPDAVALLGDALYHGPRNPLPADYDPKGVAALLN 60 Query: 61 AYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHEGRKIYATHGHGFSIDNLPPLAPGD 120 + +VAVRGNCD+EVDQML+++PMM D+ +L GR+++ THGH +S DNLPPLA GD Sbjct: 61 GWKNRIVAVRGNCDSEVDQMLIEYPMMSDFAWVLAGGRRLFLTHGHRYSPDNLPPLADGD 120 Query: 121 VFIQGHTHLP---VADVKEG--ITVLNPGSISLPKENNPNSYAILENGEFIIKDFAGKIV 175 VF+ GHTH+P A + G + + NPGS SLPK SYA+LE+G F + G ++ Sbjct: 121 VFVYGHTHVPQTGTAALAGGGVVHLWNPGSCSLPKSGYAASYAVLEDGVFRVCGLDGSVL 180 >dbj|BAA77455.1| orf2dG [Clostridium histolyticum] Length = 180 Score = 187 bits (474), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 88/180 (48%), Positives = 118/180 (65%) Query: 1 MKLMFISDIHGSLYWXXXXXXXXXXXXPHTLVVLGDFLYHGPRNPLPDGYDPQGVAARLN 60 MK+MFISDIHGSLY+ + +LGD LYHGPRN LP Y+P+ VA LN Sbjct: 1 MKIMFISDIHGSLYFLNKALERFEEEKADYIGILGDVLYHGPRNDLPKEYNPKDVAKILN 60 Query: 61 AYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHEGRKIYATHGHGFSIDNLPPLAPGD 120 Y ++AVRGNCD+EVDQML+ +PM+ DY +I +KI+ THGH F+ DN+P GD Sbjct: 61 RYKNKIIAVRGNCDSEVDQMLIDYPMLSDYSIIFFNNKKIFLTHGHIFNKDNMPHFNIGD 120 Query: 121 VFIQGHTHLPVADVKEGITVLNPGSISLPKENNPNSYAILENGEFIIKDFAGKIVKQIRL 180 + I GHTH+P + +G+T +NPGSIS+PK + NSY IL F IK+ GK++ + + Sbjct: 121 IMISGHTHIPSIEHIDGVTFINPGSISIPKGGSENSYGILNEDGFSIKNLNGKVILTLNI 180 >ref|NP_797195.1| conserved hypothetical protein [Vibrio parahaemolyticus RIMD 2210633] dbj|BAC59079.1| conserved hypothetical protein [Vibrio parahaemolyticus] Length = 181 Score = 178 bits (451), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 84/181 (46%), Positives = 118/181 (65%), Gaps = 1/181 (0%) Query: 1 MKLMFISDIHGSLYWXXXXXXXXXXXXPHTLVVLGDFLYHGPRNPLPDGYDPQGVAARLN 60 MKL F SD+HGSL LV+LGD L HGPRNP+P+GY+P VA LN Sbjct: 1 MKLFFASDLHGSLPATEETIKLFLASGAKHLVLLGDTLNHGPRNPIPEGYNPPQVAELLN 60 Query: 61 AYDKSLVAVRGNCDAEVDQMLLQFPMMGDYVLILHE-GRKIYATHGHGFSIDNLPPLAPG 119 Y + +++VRGNCD+EVDQMLL FPMM DY +L E G++I+ THGH ++ D PPL G Sbjct: 61 QYSQQIISVRGNCDSEVDQMLLNFPMMMDYAWVLLESGKRIFLTHGHLYNSDKRPPLREG 120 Query: 120 DVFIQGHTHLPVADVKEGITVLNPGSISLPKENNPNSYAILENGEFIIKDFAGKIVKQIR 179 D+ GHTH+PVA++KEG+ + NPGS++ P+ P S+ +L+ ++ G+++ Q Sbjct: 121 DIIAHGHTHVPVAEMKEGVVIFNPGSMTFPRNGLPRSFGLLDGNILSVQTPEGEVLAQTT 180 Query: 180 L 180 + Sbjct: 181 I 181 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.143 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 282,540,823 Number of extensions: 11834355 Number of successful extensions: 24268 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 24250 Number of HSP's successfully gapped: 23 Length of query: 180 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 62 Effective length of database: 436,855,896 Effective search space: 27085065552 Effective search space used: 27085065552 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)