BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0033.p (181 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_795121.1| carbon-nitrogen hydrolase family protein [Pseud... 239 3e-62 ref|ZP_00128044.1| COG0388: Predicted amidohydrolase [Pseudomona... 238 7e-62 ref|NP_248984.1| probable hydratase [Pseudomonas aeruginosa PA01... 235 3e-61 gb|EAJ39397.1| unknown [environmental sequence] 229 2e-59 ref|ZP_00092180.1| COG0388: Predicted amidohydrolase [Azotobacte... 226 2e-58 >ref|NP_795121.1| carbon-nitrogen hydrolase family protein [Pseudomonas syringae pv. tomato str. DC3000] gb|AAO58816.1| carbon-nitrogen hydrolase family protein [Pseudomonas syringae pv. tomato str. DC3000] Length = 292 Score = 239 bits (609), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 110/165 (66%), Positives = 124/165 (75%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D++ NI AE LVR FETPYFCQK +DY AT LE N A Sbjct: 12 MACSWDLEANIETAEKLVREAAAKGAQIILIQELFETPYFCQKPNADYLQLATTLESNVA 71 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HFQ IAKELQVVLPISF+E AR+N++A+IDADGT LG YRKSHIPDGPGY EK+Y Sbjct: 72 IKHFQKIAKELQVVLPISFFELAGRARFNTIAIIDADGTNLGIYRKSHIPDGPGYHEKYY 131 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW TRYAKIGVG+CWDQW+PE AR M+L GAEILFY Sbjct: 132 FNPGDTGFKVWQTRYAKIGVGICWDQWFPECARSMALQGAEILFY 176 >ref|ZP_00128044.1| COG0388: Predicted amidohydrolase [Pseudomonas syringae pv. syringae B728a] Length = 292 Score = 238 bits (606), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 110/165 (66%), Positives = 123/165 (74%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D++ NI AE LVR FETPYFCQK DY AT LE N A Sbjct: 12 MACSWDLEANIETAEKLVREAAAKGAQIILIQELFETPYFCQKPNPDYLQLATTLESNVA 71 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HFQ IAKELQVVLPISF+E AR+NS+A+IDADG+ LG YRKSHIPDGPGY EK+Y Sbjct: 72 IKHFQKIAKELQVVLPISFFELAGRARFNSIAIIDADGSNLGIYRKSHIPDGPGYHEKYY 131 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW TRYAKIGVG+CWDQW+PE AR M+L GAEILFY Sbjct: 132 FNPGDTGFKVWQTRYAKIGVGICWDQWFPECARSMALQGAEILFY 176 >ref|NP_248984.1| probable hydratase [Pseudomonas aeruginosa PA01] pir||G83608 probable hydratase PA0293 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG03682.1| N-carbamoylputrescine amidohydrolase [Pseudomonas aeruginosa PAO1] Length = 292 Score = 235 bits (600), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 107/165 (64%), Positives = 125/165 (75%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D NI +AE LVR FETPYFCQK DY AT +E N A Sbjct: 12 MACSWDRPANIARAEKLVRQAAARGAQIILIQELFETPYFCQKPNPDYLQLATTVEENAA 71 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HFQA+A+ELQVVLPISF+E+ AR+NS+AVIDADG LG YRKSHIPDGPGY EK+Y Sbjct: 72 IAHFQALARELQVVLPISFFERAGRARFNSIAVIDADGGNLGVYRKSHIPDGPGYHEKYY 131 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW TRYA+IGVG+CWDQW+PE+AR M+L+GAE+LFY Sbjct: 132 FNPGDTGFKVWQTRYARIGVGICWDQWFPESARSMALLGAELLFY 176 >gb|EAJ39397.1| unknown [environmental sequence] Length = 293 Score = 229 bits (584), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 104/165 (63%), Positives = 121/165 (73%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D NI AE LVR FE PYFCQK +DY AT + N A Sbjct: 12 MACSWDTPANIANAERLVRQAAAQGAQIILIQELFEAPYFCQKPNADYTQLATTVAENPA 71 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HFQ +A ELQVVLPISF+E+ AR+NS+A+IDADG+ LG YRKSHIPDGPGY EK+Y Sbjct: 72 IQHFQKVAAELQVVLPISFFERAGRARFNSIAIIDADGSNLGIYRKSHIPDGPGYHEKYY 131 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW+T YA+IGVG+CWDQW+PE AR M+LMGAEILFY Sbjct: 132 FNPGDTGFKVWDTAYARIGVGICWDQWFPECARSMALMGAEILFY 176 >ref|ZP_00092180.1| COG0388: Predicted amidohydrolase [Azotobacter vinelandii] Length = 286 Score = 226 bits (575), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 101/165 (61%), Positives = 119/165 (72%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D NI AE LVR FETPYFCQK DY AT +E N A Sbjct: 12 MACSWDRAANIANAERLVREAAARGAQVILLQELFETPYFCQKPNPDYLQLATTVEENPA 71 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HF+ IA ELQVVLPISFYE+ AR+NS+A++DADG +LG YRKSHIPDGPGY EK+Y Sbjct: 72 IRHFRKIAAELQVVLPISFYERAGQARFNSIAILDADGRMLGLYRKSHIPDGPGYHEKYY 131 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW TR+ +IGV +CWDQW+PE+AR M+L GAE+L Y Sbjct: 132 FNPGDTGFKVWQTRHGRIGVAICWDQWFPESARSMALQGAELLLY 176 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.138 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 272,530,618 Number of extensions: 11162311 Number of successful extensions: 20584 Number of sequences better than 1.0e-05: 330 Number of HSP's gapped: 20632 Number of HSP's successfully gapped: 330 Length of query: 181 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 63 Effective length of database: 436,855,896 Effective search space: 27521921448 Effective search space used: 27521921448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)