BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0004.p (1044 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_622722.1| Diguanylate cyclase/phosphodiesterase domain 2 ... 432 e-119 ref|NP_953065.1| sensory box/GGDEF family protein [Geobacter sul... 383 e-104 ref|ZP_00060071.1| COG2200: FOG: EAL domain [Clostridium thermoc... 382 e-104 ref|NP_953557.1| sensory box/GGDEF family protein [Geobacter sul... 371 e-101 ref|ZP_00042687.1| COG2200: FOG: EAL domain [Magnetococcus sp. M... 370 e-101 >ref|NP_622722.1| Diguanylate cyclase/phosphodiesterase domain 2 (EAL) [Thermoanaerobacter tengcongensis] gb|AAM24326.1| Diguanylate cyclase/phosphodiesterase domain 2 (EAL) [Thermoanaerobacter tengcongensis] Length = 793 Score = 432 bits (1110), Expect = e-119, Method: Compositional matrix adjust. Identities = 229/596 (38%), Positives = 342/596 (57%), Gaps = 39/596 (6%) Query: 443 NKLEASERRFRLASEGSGAYMWELDWESGMYNLSERWYEVMGYTKEEINSFEDGVLSIIH 502 ++L+ SE R+RL EG+ +W+ D + +SERW +++G KE +N++ + + IH Sbjct: 198 DELKRSEERYRLVVEGASDGLWDWDLITDTAYISERWKDIIGLDKEVVNNYYETWIKFIH 257 Query: 503 PDDQEPARQARQEHLAGLTPIYEIEYRMRTKDNRYVWFEVRGKAIVDPKDKIVLFLGSLI 562 P D + + HL TP Y EYR++TKD RY+W RGKA+ D + + + GS Sbjct: 258 PRDIKIVINNLRNHLEKKTPFYLCEYRIKTKDGRYIWVLSRGKALWDEEGRAIRIAGSHT 317 Query: 563 DISKRKQAEFQLGNSYQELEATYEQLTATQEELVGQYDMLLENQKNMHRLAYMDSLSSLP 622 DI++RKQ E +L +Y +A+ D L+ LP Sbjct: 318 DITERKQMESKL-----------------------KY------------MAFYDPLTGLP 342 Query: 623 NRILLLDTME---SYFHRPGGKAALLFVDTDNFKYINDTLGHKSGDILIRQASERLQSVM 679 R + +D ++ + R G K A+ FVD DNFK IND+LGH GD L+++ + +L++ + Sbjct: 343 RREVFMDRLKIAMADAKRNGKKLAVFFVDLDNFKTINDSLGHHIGDRLLKKVARKLKACL 402 Query: 680 REGDMLSRLGGDEFVVFIKDVEDRAEVLNLAESILRSFRRSFLIGESNLYISVSIGISFY 739 RE D +SR+GGDEFV+ + D+ + + +A IL F + LY+++SIGI+ Y Sbjct: 403 RESDTVSRVGGDEFVILLPDIINIDDTNKVANRILELFNQPIKFNNYELYVTISIGIAIY 462 Query: 740 PEDGETTEEILMNADVAMYRAKEEGKNTFVVYDKSMHMSFNERMNIEKHLRSAMNNNEFE 799 P+ G+ I+ NAD+AMY+AK +GKN+F +D I + LR A+ +F Sbjct: 463 PDHGKNERAIVKNADIAMYKAKRDGKNSFQYFDSITKKEVEIASTIRRDLRFALEKGQFF 522 Query: 800 LHYQPQVEIKTGLITGFEALIRWNSPVLGFVSPLSFIKIAEDSRLIIYIGEWVLREASAF 859 LHYQP V+ +TG + EAL+RW P G +SP+ FI IAE++R II IGEWVLR A Sbjct: 523 LHYQPIVDARTGKVVSMEALLRWQHPKKGLISPIHFIPIAEETRQIISIGEWVLRTACKQ 582 Query: 860 MKSMNDRTGLPYKISVNISIIQLLQDDFVDIVLDSLEESGLAPNCLELEITESIFMESFE 919 MK + + +SVN+S+ QL Q DF +V D L E L P LELEITE++ +E+ + Sbjct: 583 MKEWRNMGYYGFSVSVNVSVHQLQQPDFAKVVFDILNEIELEPRFLELEITETVLIEAMD 642 Query: 920 NTVSKLEFLKSRGIRIALDDFGTGYSSLSYLQQLPISTLKMDKIFIDSLADQGYSQSFVQ 979 V L LK G++I +DDFGT SS YLQ+ + LK+D+ F+ + + +++ V Sbjct: 643 TVVRNLYRLKEMGVKIIIDDFGTENSSFKYLQKFTVDGLKIDRSFVSEIKVE-VNKAIVD 701 Query: 980 TIIILGHKMGLDVVAEGVEDADQLAFLKEADCDKVQGYLISRPVPQRGVIELLEPQ 1035 I LGH+M L V AEGVE +QL L E+ CDKVQGY S+P+P I +++ + Sbjct: 702 AITFLGHRMKLGVTAEGVETKEQLDCLVESGCDKVQGYYFSKPLPPDEAIRMVQKE 757 >ref|NP_953065.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] gb|AAR35392.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] Length = 799 Score = 383 bits (984), Expect = e-104, Method: Compositional matrix adjust. Identities = 220/563 (39%), Positives = 325/563 (57%), Gaps = 46/563 (8%) Query: 475 LSERWYEVMGYTKEEINSFEDGVLSIIHPDDQEPARQARQEHLAG--LTPIYEIEYRMRT 532 ++E W +V+GYT EE E IIHPD +E + LAG L+P +E + Sbjct: 262 VNEAWQQVLGYTAEEAK--ELSFFGIIHPDSRERCNAELERILAGDVLSP---LEATLVA 316 Query: 533 KDNRYVWFEVRGKAIVDPKDKIVLFLGSLIDISKRKQAEFQLGNSYQELEATYEQLTATQ 592 K R + E VD + ++ G DI++RKQ E QL Sbjct: 317 KGGRSIIVEGSLTCGVDGEGSTAVW-GICRDITERKQVEEQL------------------ 357 Query: 593 EELVGQYDMLLENQKNMHRLAYMDSLSSLPNRILLLDTME---SYFHRPGGKAALLFVDT 649 +RLA+ D+L+ LPNRIL LD ++ ++ +R AL+F+D Sbjct: 358 -----------------YRLAHHDTLTGLPNRILFLDRLQQAKAHANRYRHHVALMFLDL 400 Query: 650 DNFKYINDTLGHKSGDILIRQASERLQSVMREGDMLSRLGGDEFVVFIKDVEDRAEVLNL 709 D FK INDTLGH GD L+++ ++RL +RE D ++R+GGDEF V + +++ +V + Sbjct: 401 DRFKIINDTLGHPVGDKLLQEVAKRLVDCVREVDTVARIGGDEFTVVLVNIDSVEDVKRV 460 Query: 710 AESILRSFRRSFLIGESNLYISVSIGISFYPEDGETTEEILMNADVAMYRAKEEGKNTFV 769 A+ IL+S F++ LYI+ SIGI+ YP D E + ++ AD+AMY AK EG+NTF Sbjct: 461 AQKILKSLSVPFMVDSFELYITTSIGIALYPGDDENPDNLVKKADIAMYHAKGEGRNTFK 520 Query: 770 VYDKSMHMSFNERMNIEKHLRSAMNNNEFELHYQPQVEIKTGLITGFEALIRWNSPVLGF 829 Y M + ++R+ +E LR A++N EF ++YQP+V+I T IT EAL+RW P LG Sbjct: 521 FYVPKMDANADKRLLLETSLRKALDNEEFRVYYQPKVDITTKQITAMEALLRWQHPTLGL 580 Query: 830 VSPLSFIKIAEDSRLIIYIGEWVLREASAFMKSMNDRTGLPYKISVNISIIQLLQDDFVD 889 V+P FI +AE++ LI+ IGEWVLR A + + P K++VN+S Q Q + +D Sbjct: 581 VAPSEFIPLAEETGLIVPIGEWVLRAACLQNRKWLEMGLPPMKVAVNLSGYQFQQPNLLD 640 Query: 890 IVLDSLEESGLAPNCLELEITESIFMESFENTVSKLEFLKSRGIRIALDDFGTGYSSLSY 949 I+ L ++GL P LELEITES+ M++ + V+ L ++ GI I++DDFGTGYSSL++ Sbjct: 641 IIRTVLADTGLDPGLLELEITESVIMQNPDFAVAVLAEIRDLGIHISIDDFGTGYSSLAH 700 Query: 950 LQQLPISTLKMDKIFIDSLADQGYSQSFVQTIIILGHKMGLDVVAEGVEDADQLAFLKEA 1009 L++ I+TLK+DK F+ + + II +G+ + L V+AEGVE QL FL E Sbjct: 701 LKRFSINTLKIDKSFVRDVEINSADAAIATAIIAMGNSLNLKVIAEGVETEGQLTFLSEN 760 Query: 1010 DCDKVQGYLISRPVPQRGVIELL 1032 CD+VQG+L S P+P V + L Sbjct: 761 KCDEVQGFLFSTPMPSEDVADFL 783 >ref|ZP_00060071.1| COG2200: FOG: EAL domain [Clostridium thermocellum ATCC 27405] Length = 862 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 190/431 (44%), Positives = 275/431 (63%), Gaps = 3/431 (0%) Query: 609 MHRLAYMDSLSSLPNRILLLDTMESYF---HRPGGKAALLFVDTDNFKYINDTLGHKSGD 665 ++ + Y D L+ LPNR L D ++ R AL+ +D DNFK INDTLGH GD Sbjct: 422 LYNMEYYDPLTGLPNRSLFSDRLKMALAQSKRNNEMCALIILDLDNFKAINDTLGHSVGD 481 Query: 666 ILIRQASERLQSVMREGDMLSRLGGDEFVVFIKDVEDRAEVLNLAESILRSFRRSFLIGE 725 +L++Q +E+++ +RE D ++R+GGDEF++ ++D + A+ IL+ F++ +++ Sbjct: 482 MLLKQVAEKIKGYLREVDTVARIGGDEFIIIQPQIKDINDATRAADRILKKFQQPWILEG 541 Query: 726 SNLYISVSIGISFYPEDGETTEEILMNADVAMYRAKEEGKNTFVVYDKSMHMSFNERMNI 785 YI+ S+GI YP GE + I NAD A+YRAKE G+N + +Y +SM+ +R++I Sbjct: 542 KEYYITASMGIGIYPNGGEDPQTIFKNADTALYRAKELGRNNYQLYTESMNQKVLQRLDI 601 Query: 786 EKHLRSAMNNNEFELHYQPQVEIKTGLITGFEALIRWNSPVLGFVSPLSFIKIAEDSRLI 845 E LR A+ EF L YQPQ++IKTG I GFEAL+RW P G + P+ FI +AEDS LI Sbjct: 602 ENSLRRAIEKEEFVLFYQPQIDIKTGKIVGFEALLRWYHPDYGLMPPMEFIPVAEDSGLI 661 Query: 846 IYIGEWVLREASAFMKSMNDRTGLPYKISVNISIIQLLQDDFVDIVLDSLEESGLAPNCL 905 + IGEWVL A K + P+ ISVN+S Q + + V+++ +GLAP L Sbjct: 662 VVIGEWVLETACRQNKKWIECGLEPHLISVNLSARQFQRSNIVEVIDRIRSSTGLAPELL 721 Query: 906 ELEITESIFMESFENTVSKLEFLKSRGIRIALDDFGTGYSSLSYLQQLPISTLKMDKIFI 965 ELEITES M+ T+ L L+ +GIR++LDDFGTGYSSL+YL+QLPI TLK+DK F+ Sbjct: 722 ELEITESTAMQDLSFTIDVLNQLRKKGIRVSLDDFGTGYSSLNYLRQLPIDTLKIDKSFV 781 Query: 966 DSLADQGYSQSFVQTIIILGHKMGLDVVAEGVEDADQLAFLKEADCDKVQGYLISRPVPQ 1025 + ++ +T+I L HK+ L VVAEGVE +QL FLK+ CDK QGYL S+P+P Sbjct: 782 QDIRANSKEEAIAKTVISLAHKLDLTVVAEGVETKEQLLFLKKEKCDKAQGYLFSKPLPA 841 Query: 1026 RGVIELLEPQR 1036 + ++L ++ Sbjct: 842 EEIEKMLRDKK 852 Score = 231 bits (590), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 128/352 (36%), Positives = 206/352 (58%), Gaps = 14/352 (3%) Query: 3 RCQLLLLISIVFMLAPPASAHAERQEIRYQAELGYPPYKYLQNGYLTGFDIDLTSMIFEK 62 +C + L +L +A+ I Y+ EL PP+K++++G ++GF+I+L IF Sbjct: 4 KCIGINLCVFSLLLIFCQVVYAKTDTIIYETELYGPPFKFIEDGEISGFEIELNQYIFSG 63 Query: 63 QSYLIHYSTGEWEGTYKRLTRGEIDTTGLMAVTEERKKDTLFSKPVFKSYISVYSRQALQ 122 Y Y WE Y++L GEIDT GL+ V EERKKD LFS V YIS+YS++ Sbjct: 64 SEYRFDYRFNTWEKVYEKLKNGEIDTCGLLVVNEERKKDILFSDTVMNIYISIYSKEK-N 122 Query: 123 EEIKLSTLKNYKVGVGKKQYSETVLRNQTDVSQYIQYATVPQALEALKKGEIDLLFENQE 182 + I + L+ Y+VGVGK+QYSE +L++ +S Y + V +A++AL +G+ID++FENQ+ Sbjct: 123 KNIGIKDLEKYRVGVGKEQYSEHILKDSVGISNYTTFVDVEEAIDALNEGKIDVIFENQD 182 Query: 183 VVDYLIVEQGLTGNIVRRLSNLYPQDVAYGISKSEPQLVTYINARLDRLQQSGAFEELYQ 242 VV++ ++++GLTG I+ + L+P VAYG+SK P+LV YIN RLD ++++G +E+LY+ Sbjct: 183 VVNHYLIKKGLTGKIIPHKTELFPVKVAYGVSKRNPELVKYINERLDSVKKNGIYEQLYR 242 Query: 243 QYFFTHSENYTSMXXXXXXXXXXXXLFLLVTVIAALRIYIRHLR-------RTIHSEQAF 295 ++F S+ Y + L+ ++A L++YIRHL+ R + + + Sbjct: 243 KHFLRPSDFYRR----KQRIRNFAAMLALLVLLALLQVYIRHLKKKISKAYRELRKQHEW 298 Query: 296 FRDVIEHSGMIVWAVHADKRIIRFNQYAASMTGLKEKDVLGANLDEIPGLSG 347 R + G V + + N M GL E+++LG LD++ LSG Sbjct: 299 LRITLSSIGEAVITTDENGTVTFSNYEIQKMLGLSEEEILGKKLDKL--LSG 348 >ref|NP_953557.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] gb|AAR35884.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] Length = 873 Score = 371 bits (952), Expect = e-101, Method: Compositional matrix adjust. Identities = 184/422 (43%), Positives = 265/422 (62%), Gaps = 3/422 (0%) Query: 606 QKNMHRLAYMDSLSSLPNRILLLDTMESYF---HRPGGKAALLFVDTDNFKYINDTLGHK 662 Q+ + A D+L+ LPNR LL D + R G ALL D DNFK +NDTLGH Sbjct: 430 QEELLHQATHDTLTGLPNRSLLEDRLNQAVAGARRRGQMVALLMFDLDNFKVVNDTLGHT 489 Query: 663 SGDILIRQASERLQSVMREGDMLSRLGGDEFVVFIKDVEDRAEVLNLAESILRSFRRSFL 722 +GD L+R + R++S +R D +RLGGDEFV+ + ++ + + +AES F Sbjct: 490 AGDQLLRSVAARIRSFVRNCDTFARLGGDEFVIVLTNLTRMDDAVTVAESFRNLLAMPFF 549 Query: 723 IGESNLYISVSIGISFYPEDGETTEEILMNADVAMYRAKEEGKNTFVVYDKSMHMSFNER 782 +G ++I+ S+GIS +P+DG+T E ++ AD AMY KE G+N+F + + M+ N R Sbjct: 550 LGGREIFITASMGISLFPDDGDTMEALIKKADTAMYHIKESGRNSFQFFAEEMNQKVNAR 609 Query: 783 MNIEKHLRSAMNNNEFELHYQPQVEIKTGLITGFEALIRWNSPVLGFVSPLSFIKIAEDS 842 + IE LR A+ E LHYQP++E TG + G EAL+RWNSP +G +SP FI IAEDS Sbjct: 610 LTIETGLRRALEKGEMLLHYQPRLEPGTGAVVGMEALVRWNSPDMGLISPADFIPIAEDS 669 Query: 843 RLIIYIGEWVLREASAFMKSMNDRTGLPYKISVNISIIQLLQDDFVDIVLDSLEESGLAP 902 LI+ IG+WVL A + ++SVNIS Q + DFVD V+ +E+SGL+P Sbjct: 670 GLIVEIGDWVLATACCQALEWHRMGHDSLRVSVNISARQFVGHDFVDRVVRIIEQSGLSP 729 Query: 903 NCLELEITESIFMESFENTVSKLEFLKSRGIRIALDDFGTGYSSLSYLQQLPISTLKMDK 962 + +ELE+TE+ + + TV + L+ RGI I++DDFGTGYSSL+YL++ P+ LK+DK Sbjct: 730 HFVELELTETALTHNVDETVKIMNRLRERGITISIDDFGTGYSSLNYLKRFPVDVLKIDK 789 Query: 963 IFIDSLADQGYSQSFVQTIIILGHKMGLDVVAEGVEDADQLAFLKEADCDKVQGYLISRP 1022 FID + + + V TII + H M + VVAEGVE A+Q+ L E +C+++QG+ SRP Sbjct: 790 SFIDDMVKRREDAAIVATIIGIAHHMHMKVVAEGVETAEQMKLLMEGNCEEIQGFYFSRP 849 Query: 1023 VP 1024 +P Sbjct: 850 LP 851 >ref|ZP_00042687.1| COG2200: FOG: EAL domain [Magnetococcus sp. MC-1] Length = 1278 Score = 370 bits (951), Expect = e-101, Method: Compositional matrix adjust. Identities = 208/561 (37%), Positives = 318/561 (56%), Gaps = 41/561 (7%) Query: 467 DWESGMYNLSERWYEVMGYTKEEINSFEDGVLSIIHPDDQEPARQARQEHLAGLTPIYEI 526 D + + +++ + +MGYT++EI +L D+Q Q E L+ EI Sbjct: 733 DAQGDITDVNPAYQRIMGYTRDEIVGQNPSILKSGRHDEQ-FYEQMWNEMLSEGHWQGEI 791 Query: 527 EYRMRTKDNRYVWFEVRGKAIVDPKDKIVLFLGSLIDISKRKQAEFQLGNSYQELEATYE 586 R ++ + W + AI D + I F+G +DI+++K+ E QL Sbjct: 792 WDRRKSGEIFPKWLTI--DAIRDEQGVISNFVGLFMDITQQKETEEQL------------ 837 Query: 587 QLTATQEELVGQYDMLLENQKNMHRLAYMDSLSSLPNRILLLDTMESYFH---RPGGKAA 643 +LAY D L+ LPNR L D +E R G Sbjct: 838 -----------------------EKLAYYDPLTELPNRALFRDRLEQEMRLSARGGKSLG 874 Query: 644 LLFVDTDNFKYINDTLGHKSGDILIRQASERLQSVMREGDMLSRLGGDEFVVFIKDVEDR 703 L F+D D FKY+ND+LGH GD L+ Q + R++ +R D ++RLGGDEF V + +E Sbjct: 875 LFFIDLDRFKYVNDSLGHDKGDDLLIQVAGRIKHHLRTSDTVARLGGDEFTVILPGIEQT 934 Query: 704 AEVLNLAESILRSFRRSFLIGESNLYISVSIGISFYPEDGETTEEILMNADVAMYRAKEE 763 +V +A +I++S F++ ++I SIGI+ +P+DGE E + +AD AMYRAKEE Sbjct: 935 NDVARIAGAIVKSLGEVFVLQGHEVFIGGSIGIAMHPDDGEDYETLTKHADTAMYRAKEE 994 Query: 764 GKNTFVVYDKSMHMSFNERMNIEKHLRSAMNNNEFELHYQPQVEIKTGLITGFEALIRWN 823 G+ T+ + M+ R+++E+ LR A+ N+ LHYQP+V++K ITG EAL+RWN Sbjct: 995 GRGTYKFFTSDMNEINGRRLSLERALRQAVECNQLTLHYQPKVDVKQCRITGMEALVRWN 1054 Query: 824 SPVLGFVSPLSFIKIAEDSRLIIYIGEWVLREASAFMKSMNDRTGLPYKISVNISIIQLL 883 P G VSP+ FI IAE++ LII IGEWV+++A K + + P ++++N+S Q Sbjct: 1055 HPEQGLVSPVDFIPIAEETGLIITIGEWVMQQACMDAKRLARSSRHPLQVAINLSAKQFQ 1114 Query: 884 QDDFVDIVLDSLEESGLAPNCLELEITESIFMESFENTVSKLEFLKSRGIRIALDDFGTG 943 V + SL++SGLAP LE+EITES+ M T+ ++ L++ G+++++DDFGTG Sbjct: 1115 DALLVAKLERSLQQSGLAPELLEVEITESLAMGDVNRTIEQIGELRALGVKVSIDDFGTG 1174 Query: 944 YSSLSYLQQLPISTLKMDKIFIDSLADQGYSQSFVQTIIILGHKMGLDVVAEGVEDADQL 1003 YSSLSYL++LP++ LK+DK FI L + V +I+ + +GL VVAEGVE+ Q+ Sbjct: 1175 YSSLSYLKKLPLNALKIDKSFIQDLYLDSDDTAIVSSILSMASALGLSVVAEGVENQAQI 1234 Query: 1004 AFLKEADCDKVQGYLISRPVP 1024 FL E +C +QGY S+P+P Sbjct: 1235 HFLCEKECPTIQGYFFSKPLP 1255 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,443,383,629 Number of extensions: 59527425 Number of successful extensions: 173870 Number of sequences better than 1.0e-05: 3202 Number of HSP's gapped: 170343 Number of HSP's successfully gapped: 3278 Length of query: 1044 Length of database: 757,237,578 Length adjustment: 136 Effective length of query: 908 Effective length of database: 387,984,114 Effective search space: 352289575512 Effective search space used: 352289575512 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)