BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0002.p (349 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_213011.1| hypothetical protein [Aquifex aeolicus] >gi|751... 129 8e-29 ref|ZP_00065584.1| COG2199: FOG: GGDEF domain [Microbulbifer deg... 124 2e-27 ref|NP_213183.1| hypothetical protein [Aquifex aeolicus] >gi|751... 123 6e-27 ref|ZP_00140319.1| COG2199: FOG: GGDEF domain [Pseudomonas aerug... 123 8e-27 ref|NP_254174.1| hypothetical protein [Pseudomonas aeruginosa PA... 122 1e-26 >ref|NP_213011.1| hypothetical protein [Aquifex aeolicus] pir||H70302 conserved hypothetical protein aq_035 - Aquifex aeolicus gb|AAC06416.1| hypothetical protein [Aquifex aeolicus VF5] Length = 431 Score = 129 bits (325), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 64/169 (37%), Positives = 102/169 (60%), Gaps = 2/169 (1%) Query: 182 QNIIMDRLSKVDPLTELYNHKTFHEYLGWLIEHQQSNPFPMQLAVLDIDNFKKVNDQYGH 241 + + +R++ DPLT LYN + F E + + + + ++DIDNFKK+ND YGH Sbjct: 256 EKTLYERMALTDPLTGLYNRRVFTEMAEKELAKAKRYGYNFSILMIDIDNFKKINDTYGH 315 Query: 242 WVGDIVLRQVAGIVLKHIGADDFAARYGGEEFVVILTAKPLEDSRRIMTQILHGIAALPV 301 VGD+VL++++ I+ +++ D AR+GGEEF+V+L+ L + + Q+ I P+ Sbjct: 316 DVGDLVLKKISEILKRNVRGADLVARFGGEEFIVMLSNTNLNGAVKKAEQLRRMIEQTPI 375 Query: 302 AEMDGNS--VTVSIGMHDYAGTDSKSSTFQQADDALYEAKKTGKNKIVV 348 +G VTVSIG+ Y G +S ++AD ALYEAK+ GKN++ V Sbjct: 376 ELPNGEKLRVTVSIGVSTYRGHESLEELIKEADQALYEAKRKGKNRVEV 424 >ref|ZP_00065584.1| COG2199: FOG: GGDEF domain [Microbulbifer degradans 2-40] Length = 565 Score = 124 bits (312), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 79/228 (34%), Positives = 122/228 (53%), Gaps = 12/228 (5%) Query: 128 LDTSVYDNSLRIQNMIIAFILTGTALAGFALIARGRDLMQSLESSLKSEQDLRIQNIIMD 187 L ++V D+ IQN + F A + +L A+ + LM +++ + + R Q Sbjct: 343 LQSTVSDHIRTIQNALSTFKQAQPAPSQ-SLSAQLKALMDRVKTVEEESEKSRAQLEEQR 401 Query: 188 RLSKVDPLT-----ELYNHKTFHEYLGWLIEHQQSNPFPMQLAVLDIDNFKKVNDQYGHW 242 + DPLT E YN + FHE + + P+ LAV D+D+FKK+ND +GH Sbjct: 402 YKATHDPLTSLPNREAYNERAFHELHRF-----KRYGHPLTLAVCDLDHFKKINDTFGHQ 456 Query: 243 VGDIVLRQVAGIVLKHIGADDFAARYGGEEFVVILTAKPLEDSRRIMTQILHGIAALPVA 302 GD VL+ VA +V + DF ARYGGEEFV+++ E ++ ++ ++ IA P Sbjct: 457 AGDKVLKLVAQVVSTRLRNVDFVARYGGEEFVLVMPETDAEQAKTVLDKMRAAIAKTPFR 516 Query: 303 EMDGN-SVTVSIGMHDYAGTDSKSSTFQQADDALYEAKKTGKNKIVVV 349 D ++T+S G+ + G DS S F +AD ALYEAK G+N+ V+ Sbjct: 517 FKDSPVTITMSFGIVQFRGEDSVESAFARADKALYEAKANGRNQCVIA 564 >ref|NP_213183.1| hypothetical protein [Aquifex aeolicus] pir||C70324 conserved hypothetical protein aq_265 - Aquifex aeolicus gb|AAC06589.1| hypothetical protein [Aquifex aeolicus VF5] Length = 338 Score = 123 bits (309), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 64/160 (40%), Positives = 96/160 (60%), Gaps = 3/160 (1%) Query: 191 KVDPLTELYNHKTFHEYLGWLIEHQQSNPFPMQLAVLDIDNFKKVNDQYGHWVGDIVLRQ 250 KVDPLT L N + L ++E P +A++DIDNFKK+ND YGH GD VL++ Sbjct: 178 KVDPLTGLLNRRVLPYILRDVLELSLYTETPFSIAMVDIDNFKKINDTYGHLFGDKVLKE 237 Query: 251 VAGIVLKHIGADDFAARYGGEEFVVILTAKPLEDSRRIMTQILHGIAALPVAEMDGN-SV 309 VA I+ K++ D+ RYGGEEF++++ + L+D+ RI+ +I + P+ V Sbjct: 238 VAKIIKKNLRRSDYVFRYGGEEFLILMPSTELKDAVRILEKIRKEVENTPICWQGKEIRV 297 Query: 310 TVSIGMHD--YAGTDSKSSTFQQADDALYEAKKTGKNKIV 347 T+S+G+ Y G S + AD+ LY AK+TGKN++V Sbjct: 298 TISVGVCSDVYNGLKSPEEYIKCADEKLYLAKRTGKNRVV 337 >ref|ZP_00140319.1| COG2199: FOG: GGDEF domain [Pseudomonas aeruginosa UCBPP-PA14] Length = 253 Score = 123 bits (308), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 69/179 (38%), Positives = 104/179 (58%), Gaps = 6/179 (3%) Query: 176 EQDLR-IQNIIMDRLSKV--DPLTELYNHKTFHEYLGWLIEHQQSNPFPMQLAVLDIDNF 232 EQD + + + D+ K DPLT L N E L + + + + LAVLDID+F Sbjct: 76 EQDAKAFHSHLEDQRQKALTDPLTGLPNRAALSERLEQEVARRHRDGGDLLLAVLDIDHF 135 Query: 233 KKVNDQYGHWVGDIVLRQVAGIVLKHIGADDFAARYGGEEFVVILTAKPLEDSRRIMTQI 292 K++ND +GH GD VL+ +AG + K + DF AR+GGEEFVV+L A LE R+++ ++ Sbjct: 136 KRINDDFGHLAGDKVLKIIAGELRKRLRQADFIARFGGEEFVVLLPATSLEAGRQLLERL 195 Query: 293 LHGIAALPVAEMDGN--SVTVSIGMHDYAGTDSKSSTFQQADDALYEAKKTGKNKIVVV 349 IAA P G S+T S G+ + G ++ F++AD ALY AK+ G++++ V Sbjct: 196 RAAIAACPF-HFKGEPLSITCSAGITAFEGNEAGEVVFERADQALYRAKRAGRDRLEVA 253 >ref|NP_254174.1| hypothetical protein [Pseudomonas aeruginosa PA01] pir||F82960 hypothetical protein PA5487 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG08872.1| hypothetical protein PA5487 [Pseudomonas aeruginosa PAO1] Length = 671 Score = 122 bits (307), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 69/179 (38%), Positives = 105/179 (58%), Gaps = 6/179 (3%) Query: 176 EQDLR-IQNIIMDRLSKV--DPLTELYNHKTFHEYLGWLIEHQQSNPFPMQLAVLDIDNF 232 EQD + + + D+ K DPLT L N E L + + + + LAVLDID+F Sbjct: 494 EQDAKAFHSHLEDQRQKALTDPLTGLPNRAALSERLEQEVARRHRDGGDLLLAVLDIDHF 553 Query: 233 KKVNDQYGHWVGDIVLRQVAGIVLKHIGADDFAARYGGEEFVVILTAKPLEDSRRIMTQI 292 K++ND +GH GD VL+ +AG + K + DF AR+GGEEFVV+L A LE R+++ ++ Sbjct: 554 KRINDDFGHLAGDKVLKIIAGELRKRLRQADFIARFGGEEFVVLLPATSLEAGRQLLERL 613 Query: 293 LHGIAALPVAEMDGN--SVTVSIGMHDYAGTDSKSSTFQQADDALYEAKKTGKNKIVVV 349 IAA P G S+T S G+ + G ++ + F++AD ALY AK+ G++++ V Sbjct: 614 RAAIAACPF-HFKGEPLSITCSAGITAFEGNEAGEAVFERADQALYRAKRAGRDRLEVA 671 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.138 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 452,766,056 Number of extensions: 17331218 Number of successful extensions: 52512 Number of sequences better than 1.0e-05: 2037 Number of HSP's gapped: 50768 Number of HSP's successfully gapped: 2037 Length of query: 349 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 223 Effective length of database: 415,135,104 Effective search space: 92575128192 Effective search space used: 92575128192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)