BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0001.p (155 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_657736.1| hypothetical protein predicted by GeneMark [Bac... 126 2e-28 ref|NP_389585.1| morphogenic protein [Bacillus subtilis] >gi|116... 124 7e-28 ref|NP_833493.1| Spore coat protein E [Bacillus cereus ATCC 1457... 122 2e-27 ref|NP_980101.1| spore coat protein E [Bacillus cereus ATCC 1098... 121 6e-27 ref|NP_243236.1| outer spore coat protein [Bacillus halodurans] ... 120 8e-27 >ref|NP_657736.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_846153.1| spore coat protein E [Bacillus anthracis str. Ames] gb|AAP27639.1| spore coat protein E [Bacillus anthracis str. Ames] Length = 180 Score = 126 bits (316), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 61/137 (44%), Positives = 82/137 (59%) Query: 5 HHPTSILGAWIINHQYEAVAAGNGIEVIGTYDVNIWYSYDKNKQTEVAKETVSYVELIPL 64 + PTSILG W+INH YEA G +E+ G YDVN WYS+D N +TEV E V+Y + + + Sbjct: 30 NEPTSILGCWVINHSYEARKNGKHVEIEGFYDVNTWYSFDGNTKTEVVTERVNYTDEVSI 89 Query: 65 SYLDPRHRAATVEVSAEATQEPNCVEAGVSPGGGSVTLRVEREFEAELVAETKVRVYVTD 124 Y D +E+ A Q PNC+EA VSP G + + VEREF E+V ETK+ V V Sbjct: 90 GYRDKNFSGDDLEIIARVIQPPNCLEALVSPNGNKIVVTVEREFVTEVVGETKICVSVNP 149 Query: 125 QSDDPEDKDYDFDGESF 141 + D+D+ D + F Sbjct: 150 EGCVESDEDFQIDDDEF 166 >ref|NP_389585.1| morphogenic protein [Bacillus subtilis] sp|P14016|COTE_BACSU Spore coat protein E pir||A31245 outer spore coat protein cotE - Bacillus subtilis emb|CAA31433.1| coat protein [Bacillus subtilis] emb|CAB13576.1| morphogenic protein [Bacillus subtilis subsp. subtilis str. 168] Length = 181 Score = 124 bits (311), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 59/122 (48%), Positives = 80/122 (65%) Query: 1 VTPPHHPTSILGAWIINHQYEAVAAGNGIEVIGTYDVNIWYSYDKNKQTEVAKETVSYVE 60 ++P P+SILG WIINH+Y+A G +E+ G YD+N+WYSY N +TEV E V YV+ Sbjct: 26 ISPEKKPSSILGGWIINHKYDAEKIGKTVEIEGYYDINVWYSYADNTKTEVVTERVKYVD 85 Query: 61 LIPLSYLDPRHRAATVEVSAEATQEPNCVEAGVSPGGGSVTLRVEREFEAELVAETKVRV 120 +I L Y D + EV A+ Q+PNC+E +SP G + ++ EREF AE+V ETKV V Sbjct: 86 VIKLRYRDNNYLDDEHEVIAKVLQQPNCLEVTISPNGNKIVVQAEREFLAEVVGETKVVV 145 Query: 121 YV 122 V Sbjct: 146 EV 147 >ref|NP_833493.1| Spore coat protein E [Bacillus cereus ATCC 14579] gb|AAP10694.1| Spore coat protein E [Bacillus cereus ATCC 14579] Length = 180 Score = 122 bits (307), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 60/137 (43%), Positives = 80/137 (58%) Query: 5 HHPTSILGAWIINHQYEAVAAGNGIEVIGTYDVNIWYSYDKNKQTEVAKETVSYVELIPL 64 + PTSILG W+INH YEA G +E+ G YDVN WYS+D N +TEV E V+Y + + + Sbjct: 30 NEPTSILGCWVINHSYEARKNGKHVEIEGFYDVNTWYSFDGNTKTEVVTERVNYTDEVSI 89 Query: 65 SYLDPRHRAATVEVSAEATQEPNCVEAGVSPGGGSVTLRVEREFEAELVAETKVRVYVTD 124 Y D +E+ A Q PNC+EA VSP G + + VEREF E+V ETK+ V V Sbjct: 90 GYRDKNFSGDDLEIIARVIQPPNCLEALVSPNGNKIVVTVEREFVTEVVGETKICVSVNP 149 Query: 125 QSDDPEDKDYDFDGESF 141 + D D+ + F Sbjct: 150 EGCVESDDDFQIADDEF 166 >ref|NP_980101.1| spore coat protein E [Bacillus cereus ATCC 10987] gb|AAS42709.1| spore coat protein E [Bacillus cereus ATCC 10987] Length = 180 Score = 121 bits (303), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 59/137 (43%), Positives = 80/137 (58%) Query: 5 HHPTSILGAWIINHQYEAVAAGNGIEVIGTYDVNIWYSYDKNKQTEVAKETVSYVELIPL 64 + PTSILG W+INH YEA G +E+ G YDVN WYS+D N +TEV E V+Y + + + Sbjct: 30 NEPTSILGCWVINHSYEARKNGKHVEIEGFYDVNTWYSFDGNTKTEVVTERVNYTDEVSI 89 Query: 65 SYLDPRHRAATVEVSAEATQEPNCVEAGVSPGGGSVTLRVEREFEAELVAETKVRVYVTD 124 Y D +E+ A Q PNC+EA VSP G + + VEREF E+V ETK+ V V Sbjct: 90 GYRDKNFSGDDLEIIARVIQPPNCLEALVSPNGNKIVVTVEREFVTEVVGETKICVSVNP 149 Query: 125 QSDDPEDKDYDFDGESF 141 + D + + + F Sbjct: 150 EGCVESDDAFQIEDDEF 166 >ref|NP_243236.1| outer spore coat protein [Bacillus halodurans] pir||B83946 outer spore coat protein cotE [imported] - Bacillus halodurans (strain C-125) dbj|BAB06089.1| outer spore coat protein [Bacillus halodurans] Length = 187 Score = 120 bits (302), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 56/122 (45%), Positives = 81/122 (66%) Query: 1 VTPPHHPTSILGAWIINHQYEAVAAGNGIEVIGTYDVNIWYSYDKNKQTEVAKETVSYVE 60 ++P P+SILG WIINH+Y A G +EV G+YD+N+W+SY N +T+VA +TVSY + Sbjct: 29 ISPSEKPSSILGCWIINHKYSADKKGENVEVQGSYDINVWFSYKNNTKTDVATQTVSYTD 88 Query: 61 LIPLSYLDPRHRAATVEVSAEATQEPNCVEAGVSPGGGSVTLRVEREFEAELVAETKVRV 120 +IPL+ D + ++V A Q+PN +EA ++ G V ++VEREF E + ETKV V Sbjct: 89 IIPLTMRDENVLSKEMDVVARPIQQPNTLEATIAESGVHVDVQVEREFVVECIGETKVAV 148 Query: 121 YV 122 V Sbjct: 149 AV 150 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.311 0.131 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 223,460,252 Number of extensions: 8097047 Number of successful extensions: 16525 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 16524 Number of HSP's successfully gapped: 6 Length of query: 155 Length of database: 757,237,578 Length adjustment: 113 Effective length of query: 42 Effective length of database: 450,431,391 Effective search space: 18918118422 Effective search space used: 18918118422 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 120 (50.8 bits)