BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0449.0009.p (554 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAA25878.1| mannanase [Bacillus circulans] 388 e-106 pir||JE0134 mannan endo-1,4-beta-mannosidase (EC 3.2.1.78) - Bac... 385 e-105 gb|AAS48170.1| mannanase [Bacillus circulans] 369 e-100 ref|ZP_00065570.1| COG2730: Endoglucanase [Microbulbifer degrada... 248 2e-64 dbj|BAA25188.1| beta-1,4-mannanase [Vibrio sp.] 230 8e-59 >dbj|BAA25878.1| mannanase [Bacillus circulans] Length = 516 Score = 388 bits (996), Expect = e-106, Method: Compositional matrix adjust. Identities = 194/322 (60%), Positives = 237/322 (73%), Gaps = 4/322 (1%) Query: 14 ILALTFILILSLSIPQKQAEAAGTSVKGFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDL 73 I + F+L+ S + Q + GFYVSG L DATG PFVMRGVNHAHTWYK+ L Sbjct: 13 IAFVAFLLMFSWT---GQLTNKAHAASGFYVSGTKLLDATGQPFVMRGVNHAHTWYKDQL 69 Query: 74 XXXXXXXXXTGANTVRIVLSNGGQWSLDSLSDVQNILALCDQYKLTAMLEVHDATGSDNA 133 TGANT+RIVL+NG +W+LD ++ V NIL LC+Q KL A+LEVHDATGSD+ Sbjct: 70 STAIPAIAKTGANTIRIVLANGHKWTLDDVNTVNNILTLCEQNKLIAVLEVHDATGSDSL 129 Query: 134 STLNAAVNYWISIKDALIGKEDRVIVNIANEWFGSWSTPAWASGYQSAIPALRAAGIKNT 193 S L+ AVNYWI IK ALIGKEDRVI+NIANEW+G+W AWA+GY+ AIP LR AG+ +T Sbjct: 130 SDLDNAVNYWIGIKSALIGKEDRVIINIANEWYGTWDGVAWANGYKQAIPKLRNAGLTHT 189 Query: 194 LVVDAAGWGQYPSSIFTSGNAVFNSDPLANTIFSIHMYEYSGGTAATVKSNIDNALAIGV 253 L+VD+AGWGQYP S+ G V N+DPL NT+FSIHMYEY+GG A+TVKSNID L + Sbjct: 190 LIVDSAGWGQYPDSVKNYGTEVLNADPLKNTVFSIHMYEYAGGNASTVKSNIDGVLNKNL 249 Query: 254 PVIIGEFGWKHSGGDVDEPAIMSYSQEKGVGWLAWSWYGNGGGVEYLDLASGPAG-TLSD 312 +IIGEFG +H+ GDVDE IMSYSQEKGVGWLAWSW GN + YLD+ + AG +L+ Sbjct: 250 ALIIGEFGGQHTNGDVDEATIMSYSQEKGVGWLAWSWKGNSSDLAYLDMTNDWAGNSLTS 309 Query: 313 WGKSVVNGPYGTKATSALNKIY 334 +G +VVNG G KATS L+ I+ Sbjct: 310 FGNTVVNGSNGIKATSVLSGIF 331 >pir||JE0134 mannan endo-1,4-beta-mannosidase (EC 3.2.1.78) - Bacillus circulans Length = 516 Score = 385 bits (988), Expect = e-105, Method: Compositional matrix adjust. Identities = 193/322 (59%), Positives = 235/322 (72%), Gaps = 4/322 (1%) Query: 14 ILALTFILILSLSIPQKQAEAAGTSVKGFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDL 73 I + F+L+ S + Q + GFYVSG L DATG PFVMRGVNHAHTWYK+ L Sbjct: 13 IAFVAFLLMFSWT---GQLTNKAHAASGFYVSGTKLLDATGQPFVMRGVNHAHTWYKDQL 69 Query: 74 XXXXXXXXXTGANTVRIVLSNGGQWSLDSLSDVQNILALCDQYKLTAMLEVHDATGSDNA 133 TGANT+RIVL+NG +W+LD ++ V NIL LC+Q KL A+LEVHDATGSD+ Sbjct: 70 STAIPAIAKTGANTIRIVLANGHKWTLDDVNTVNNILTLCEQNKLIAVLEVHDATGSDSL 129 Query: 134 STLNAAVNYWISIKDALIGKEDRVIVNIANEWFGSWSTPAWASGYQSAIPALRAAGIKNT 193 S L+ AVNYWI IK ALIGKEDRVI+NIANEW+G+W AWA+GY+ AIP LR AG+ +T Sbjct: 130 SDLDNAVNYWIGIKSALIGKEDRVIINIANEWYGTWDGVAWANGYKQAIPKLRNAGLTHT 189 Query: 194 LVVDAAGWGQYPSSIFTSGNAVFNSDPLANTIFSIHMYEYSGGTAATVKSNIDNALAIGV 253 L+VD+AGWGQYP S+ G V N+DPL NT+FSIHMYEY+GG A+TVKSNID L + Sbjct: 190 LIVDSAGWGQYPDSVKNYGTEVLNADPLKNTVFSIHMYEYAGGNASTVKSNIDGVLNKNL 249 Query: 254 PVIIGEFGWKHSGGDVDEPAIMSYSQEKGVGWLAWSWYGNGGGVEYLDLASGPAG-TLSD 312 +IIGEFG H+ GDVDE IMSYS EKGVGWLAWSW GN + YLD+ + AG +L+ Sbjct: 250 ALIIGEFGGNHTNGDVDEATIMSYSNEKGVGWLAWSWKGNSSDLAYLDMTNDWAGNSLTS 309 Query: 313 WGKSVVNGPYGTKATSALNKIY 334 +G +VVNG G KATS L+ I+ Sbjct: 310 FGNTVVNGSNGIKATSVLSGIF 331 >gb|AAS48170.1| mannanase [Bacillus circulans] Length = 493 Score = 369 bits (946), Expect = e-100, Method: Compositional matrix adjust. Identities = 172/295 (58%), Positives = 222/295 (75%), Gaps = 1/295 (0%) Query: 41 GFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDLXXXXXXXXXTGANTVRIVLSNGGQWSL 100 GFYVSG L+DA G+PFVMRG+NH H WYK+ T ANT+RIVLS+GGQW+ Sbjct: 38 GFYVSGTTLYDANGNPFVMRGINHGHAWYKDQATTAIEGIANTDANTIRIVLSDGGQWTK 97 Query: 101 DSLSDVQNILALCDQYKLTAMLEVHDATGSDNASTLNAAVNYWISIKDALIGKEDRVIVN 160 D + V+N+++L ++ L A+LEVHDATG D+ ++LN AV+YWI ++ ALIGKED VI+N Sbjct: 98 DDIQTVRNLISLAEENNLVAVLEVHDATGYDSIASLNRAVDYWIEMRSALIGKEDTVIIN 157 Query: 161 IANEWFGSWSTPAWASGYQSAIPALRAAGIKNTLVVDAAGWGQYPSSIFTSGNAVFNSDP 220 IANEWFGSW AWA GY+ AIP LR AG+ +TL+VDAAGWGQ+P SI G VFN+DP Sbjct: 158 IANEWFGSWEGAAWADGYKQAIPRLRNAGLNHTLMVDAAGWGQFPQSIHDFGREVFNADP 217 Query: 221 LANTIFSIHMYEYSGGTAATVKSNIDNALAIGVPVIIGEFGWKHSGGDVDEPAIMSYSQE 280 NT+FSIHMYEY+GG A+ V++NID L + ++IGEFG +H+ GDVDE IMSYS++ Sbjct: 218 QRNTMFSIHMYEYAGGNASQVRTNIDRVLNQDLALVIGEFGHRHTNGDVDEATIMSYSEQ 277 Query: 281 KGVGWLAWSWYGNGGGVEYLDLASGPAG-TLSDWGKSVVNGPYGTKATSALNKIY 334 +GVGWLAWSW GNG EYLDL++ AG L+ WG ++VNGPYG + TS L+ ++ Sbjct: 278 RGVGWLAWSWKGNGPEWEYLDLSNDWAGNNLTAWGNTIVNGPYGLRETSRLSTVF 332 >ref|ZP_00065570.1| COG2730: Endoglucanase [Microbulbifer degradans 2-40] Length = 561 Score = 248 bits (634), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 139/309 (44%), Positives = 194/309 (62%), Gaps = 11/309 (3%) Query: 34 AAGTSVKGFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDLXXXXXXXXXTGANTVRIVLS 93 AA T GF VSG L D G F+MRGVNH H WY N GANTVR+VLS Sbjct: 31 AASTVNAGFSVSGTQLLDDNGQAFIMRGVNHPHAWYANQ-TSSFADIASVGANTVRVVLS 89 Query: 94 NGGQWSLDSLSDVQNILALCDQYKLTAMLEVHDATGSDNAS---TLNAAVNYWISIKDAL 150 +G QW+ +S SDV N+++LC KL +LEVHD TGS AS TL A YW+ I + L Sbjct: 90 DGQQWTRNSASDVANVISLCKANKLVCVLEVHDVTGSGEASAAGTLANAAQYWVDIANVL 149 Query: 151 IGKEDRVIVNIANEWFGSWSTPA--WASGYQSAIPALRAAGIKNTLVVDAAGWGQ-YPSS 207 G+ED VI+NIANE FG+ + PA W + +++AI LRAAG+ +TL++DAA WGQ + Sbjct: 150 KGQEDYVIINIANEPFGN-NVPASNWINQHKAAIQTLRAAGLTHTLMIDAANWGQDWQQV 208 Query: 208 IFTSGNAVFNSDPLANTIFSIHMYEYSGGTAATVKSNIDNALAI-GVPVIIGEFGWKHSG 266 + + + V +D L+NT+FS+HMY+ +TV++ + L+ +P+I+GEFG H G Sbjct: 209 MLNNASEVAQADSLSNTMFSVHMYQVY-NNLSTVENYVSTFLSSHNLPLIVGEFGADHQG 267 Query: 267 GDVDEPAIMSYSQEKGVGWLAWSWYGNGGGVEYLDLASG-PAGTLSDWGKSVVNGPYGTK 325 +VDE AI+S +++ G+G+L WSW GNG LD+ + +L+ WG ++NG G K Sbjct: 268 EEVDEDAILSVAEQYGIGYLGWSWSGNGSCCGTLDITNNFNVNSLTSWGNRLINGTNGIK 327 Query: 326 ATSALNKIY 334 ATS + +Y Sbjct: 328 ATSVIASVY 336 >dbj|BAA25188.1| beta-1,4-mannanase [Vibrio sp.] Length = 395 Score = 230 bits (586), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 137/309 (44%), Positives = 192/309 (62%), Gaps = 9/309 (2%) Query: 35 AGTSVKGFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDLXXXXXXXXXTGANTVRIVLSN 94 A + GFYVS L++A GS F +RG+NHAHTWY + L TGANTVR+VLSN Sbjct: 16 ASCAHAGFYVSNGVLYEANGSAFKIRGINHAHTWYTDKLSVALSGIAATGANTVRVVLSN 75 Query: 95 GGQWSLDSLSDVQNILALCDQYKLTAMLEVHDATG---SDNASTLNAAVNYWISIKDALI 151 G +W+ + +SDV NI+ L L A+LEVHD TG +A++L++A +YWI +K+ LI Sbjct: 76 GYRWTKNDVSDVTNIINLAKANNLIAILEVHDTTGYGEESSAASLDSAADYWIELKNELI 135 Query: 152 GKEDRVIVNIANEWFGSWSTP-AWASGYQSAIPALRAAGIKNTLVVDAAGWGQ-YPSSIF 209 G+ED VI+N+ NE FG+ + AW + + SAI LR+AGI +T++VDA WGQ + + Sbjct: 136 GQEDYVIINLGNEPFGNNNDAVAWVNDHVSAIQRLRSAGINHTIMVDAPNWGQDWKGFML 195 Query: 210 TSGNAVFNSDPLANTIFSIHMYE-YSGGTAATVKSNIDNALAIGVPVIIGEFGWKHSGGD 268 + VFNSDP NTIFS+HMYE YS + +V I + G+ ++IGEF H G D Sbjct: 196 NNAQFVFNSDPKLNTIFSVHMYEVYS--SYNSVNDYISSFTNNGLVLVIGEFASTHKGAD 253 Query: 269 VDEPAIMSYSQEKGVGWLAWSWYGNGGGVEYLDLASG-PAGTLSDWGKSVVNGPYGTKAT 327 VDE +IM S+ +G++ WSW GN LD+ + + S WG ++NG G K+T Sbjct: 254 VDEGSIMERSETLSLGYIGWSWSGNDTTTSDLDIVNNWDNNSYSTWGNVLINGQNGIKST 313 Query: 328 SALNKIYTT 336 S L ++T Sbjct: 314 STLATVFTC 322 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.132 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 777,923,823 Number of extensions: 32192410 Number of successful extensions: 76128 Number of sequences better than 1.0e-05: 99 Number of HSP's gapped: 75891 Number of HSP's successfully gapped: 114 Length of query: 554 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 423 Effective length of database: 401,559,609 Effective search space: 169859714607 Effective search space used: 169859714607 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)