BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0010.p (224 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_388070.1| similar to chloramphenicol resistance protein [... 218 8e-56 ref|NP_834683.1| Chloramphenicol resistance protein [Bacillus ce... 80 4e-14 ref|NP_388461.1| similar to chloramphenicol resistance protein [... 79 1e-13 ref|NP_242982.1| chloramphenicol resistance protein [Bacillus ha... 79 1e-13 emb|CAB12399.2| ydhL [Bacillus subtilis subsp. subtilis str. 168] 78 1e-13 >ref|NP_388070.1| similar to chloramphenicol resistance protein [Bacillus subtilis] pir||A69746 chloramphenicol resistance protein homolog ybcL - Bacillus subtilis emb|CAB11965.1| ybcL [Bacillus subtilis subsp. subtilis str. 168] dbj|BAA33084.1| ybcL [Bacillus subtilis] Length = 390 Score = 218 bits (555), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 108/211 (51%), Positives = 141/211 (66%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 LGLI+M++IF T+P +GD+P+PL QQLAL K+ KVA+GL++TFFWLGGYS+AYTYLSPY Sbjct: 168 LGLIAMVVIFFTLPYTEGDKPVPLLQQLALFKKRKVAMGLSITFFWLGGYSVAYTYLSPY 227 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 L+ ISGIN +SG LLIFGIAS++GSK GG+S D+WGV TL G Sbjct: 228 LLNISGINGKLLSGVLLIFGIASLVGSKFGGYSTDKWGVPFTLVGGMTLHIVTLILLSLV 287 Query: 121 XXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQSXXXXXXXXXXXXXX 180 +L+LWSFAAWSTGPTQQ+H+ T++PE SGV+L +NQS Sbjct: 288 THSYIGVLVILILWSFAAWSTGPTQQFHLATIEPEMSGVLLSMNQSMMQFAMAVGAGIGG 347 Query: 181 XXXXXXXLSSITWIGAASVFLATSATLILYR 211 L+SITW+GA V +A A+L+++ Sbjct: 348 VFVENVSLASITWVGALGVMIAIIASLLIFN 378 >ref|NP_834683.1| Chloramphenicol resistance protein [Bacillus cereus ATCC 14579] gb|AAP11884.1| Chloramphenicol resistance protein [Bacillus cereus ATCC 14579] Length = 390 Score = 80.1 bits (196), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 47/163 (28%), Positives = 82/163 (50%), Gaps = 2/163 (1%) Query: 4 ISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPYLIT 63 I+ + +FLT ++ + + +QLA LK+ K+ +F +L G+ Y YL+P+L Sbjct: 171 IACISLFLT--KVPPTSVLSIKEQLATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKD 228 Query: 64 ISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXXXXX 123 + + ++IS IFGIA++IG GG+ AD+WG ++ Sbjct: 229 VMHVEANWISVFYFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFILPLMTFS 288 Query: 124 XXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQS 166 ++ LWS +W+ P QQ ++ + PE++G+ GLN S Sbjct: 289 FPLFIIMMGLWSMLSWAISPAQQNYLIEIAPESAGIQQGLNNS 331 >ref|NP_388461.1| similar to chloramphenicol resistance protein [Bacillus subtilis] pir||H69784 chloramphenicol resistance protein homolog ydhL - Bacillus subtilis dbj|BAA19704.1| S. lividans chloramphenicol resistance protein; P31141 (492) transmembrane [Bacillus subtilis] Length = 425 Score = 78.6 bits (192), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 46/166 (27%), Positives = 77/166 (46%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 L LISMLII + RI ++ IP +QL + K+A VT F L G+ Y Y +P+ Sbjct: 203 LALISMLIISIFFERIPAEKMIPFREQLKTIGNLKIASSHLVTMFTLAGHYTLYAYFAPF 262 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 L ++ ++S +FGI+++ G GG +DR G + ++ Sbjct: 263 LEETLHLSSFWVSICYFLFGISAVCGGPFGGALSDRLGSFKSILLVTGSFAIIMFLLPLS 322 Query: 121 XXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQS 166 V+++W +WS P QQ ++ + P++S + N S Sbjct: 323 TSSMIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPDSSDIQQSFNTS 368 >ref|NP_242982.1| chloramphenicol resistance protein [Bacillus halodurans] pir||D83914 chloramphenicol resistance protein BH2116 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05835.1| chloramphenicol resistance protein [Bacillus halodurans] Length = 286 Score = 78.6 bits (192), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 36/101 (35%), Positives = 64/101 (63%), Gaps = 3/101 (2%) Query: 1 LGLISMLII---FLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYL 57 + LIS+ +I + +P I+ ++P+P + QL ++K+ ++ GL T FW+ GY++ + Y+ Sbjct: 168 IALISLFLIISLYKLLPEIQANKPLPFTTQLQIMKDRRLMTGLGTTIFWILGYTMVFAYI 227 Query: 58 SPYLITISGINDSYISGALLIFGIASMIGSKIGGFSADRWG 98 SP L + + IS AL + G ++ IGS+ GGF+ D+WG Sbjct: 228 SPLLSREAEMPLEMISVALFVLGTSAFIGSRFGGFAVDKWG 268 >emb|CAB12399.2| ydhL [Bacillus subtilis subsp. subtilis str. 168] Length = 388 Score = 78.2 bits (191), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 46/166 (27%), Positives = 77/166 (46%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 L LISMLII + RI ++ IP +QL + K+A VT F L G+ Y Y +P+ Sbjct: 166 LALISMLIISIFFERIPAEKMIPFREQLKTIGNLKIASSHLVTMFTLAGHYTLYAYFAPF 225 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 L ++ ++S +FGI+++ G GG +DR G + ++ Sbjct: 226 LEETLHLSSFWVSICYFLFGISAVCGGPFGGALSDRLGSFKSILLVTGSFAIIMFLLPLS 285 Query: 121 XXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQS 166 V+++W +WS P QQ ++ + P++S + N S Sbjct: 286 TSSMIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPDSSDIQQSFNTS 331 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 249,190,276 Number of extensions: 7574647 Number of successful extensions: 20968 Number of sequences better than 1.0e-05: 97 Number of HSP's gapped: 20974 Number of HSP's successfully gapped: 97 Length of query: 224 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 103 Effective length of database: 428,710,599 Effective search space: 44157191697 Effective search space used: 44157191697 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)