BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0002.p (506 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00052550.1| COG0146: N-methylhydantoinase B/acetone carbo... 137 7e-31 ref|NP_214322.1| N-methylhydantoinase B [Aquifex aeolicus] >gi|7... 123 1e-26 ref|NP_614331.1| N-methylhydantoinase B [Methanopyrus kandleri A... 119 1e-25 ref|NP_343407.1| N-methyl hydantoinase B (hyuB-2) [Sulfolobus so... 114 5e-24 gb|EAJ63420.1| unknown [environmental sequence] 113 9e-24 >ref|ZP_00052550.1| COG0146: N-methylhydantoinase B/acetone carboxylase, alpha subunit [Magnetospirillum magnetotacticum] Length = 695 Score = 137 bits (344), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 96/335 (28%), Positives = 145/335 (43%), Gaps = 10/335 (2%) Query: 4 VHSIEDQIIYGKIAAVSRHAGEQLQRISRSPLIAESRAFAAGIVTEAAGLVHQIQGEAEH 63 + I+ I+ G++ AV L R S SP+I E FA + L+ Q G Sbjct: 115 IRQIDTDILGGRLNAVIDEMATVLVRTSLSPVINEVLDFACSVCDAEGRLIAQTNGITIL 174 Query: 64 LYALRASTRAMYDHFAYDLEEGDVLISADVYKGGTKGQMLTMVLPFFLEGELTLSPVIRV 123 + A+ FA + GDV IS D Y GGT +T+V P F EGEL + Sbjct: 175 TGTISCQLAAIRRKFAGAIRSGDVFISNDPYGGGTHIADITLVKPLFFEGELVCFVTVTA 234 Query: 124 QLTDLAGEYPGGYHPEAFEMWQESMRITPVKLYSGGKLQRDVQRFVLANSRTPGXXXXXX 183 +L G+ G P+A +++QE +RI +++ G Q D+ + N R P Sbjct: 235 HCNELGGKVAGSLSPDASDIFQEGLRIPALRIVREGIRQDDLIEMIAGNVRLPEMTVADL 294 Query: 184 XXXXXXXXXXXTGLRSIIQAYGLSKV----ARAIGQMKAHTQEQVLNLLPEGR---KEWC 236 LR + ++YG ++ ARA+ +A ++ + LLP G ++W Sbjct: 295 SAQLAVVRLGDLRLRELCESYGADRLKASFARALDLGEALSRAAI-RLLPNGTYSARDWI 353 Query: 237 GAADFSASKVSGRVQVRMQREGNLWKADFTGSASQVEAPFNCSEATTSACAVVAILAEHL 296 ++ +QVR+ G+ +ADFTGSA Q AP NC+ A A A A+LA Sbjct: 354 DGDGNDDERLP--IQVRVTISGDAVRADFTGSAPQRAAPINCTRAVLEAAAKTALLAVVR 411 Query: 297 ESLPVNDGLLDVFDFKLPAGSIVNPAYPSATALGY 331 P NDG P G++ + T Y Sbjct: 412 PETPANDGWFRPLTVIAPEGTVFTATDAAPTGWCY 446 >ref|NP_214322.1| N-methylhydantoinase B [Aquifex aeolicus] pir||F70465 N-methylhydantoinase (ATP-hydrolyzing) (EC 3.5.2.14) - Aquifex aeolicus gb|AAC07716.1| N-methylhydantoinase B [Aquifex aeolicus VF5] Length = 521 Score = 123 bits (308), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 90/332 (27%), Positives = 145/332 (43%), Gaps = 11/332 (3%) Query: 6 SIEDQIIYGKIAAVSRHAGEQLQRISRSPLIAESRAFAAGIVTEAAGLVHQIQGEAEHLY 65 SI ++ ++++ G LQR + SP I E R F+ + + LV Q HL Sbjct: 4 SILLEVFKNAFSSIAEEMGLVLQRTAFSPNIKERRDFSCAVFDKEGNLVAQAAHIPVHLG 63 Query: 66 ALRASTRAMYDHFAYDLEEGDVLISADVYKGGTKGQMLTMVLPFFLEGELTLSPVIRVQL 125 ++ S + F + EEGD+++ D Y GGT +T+V PFF +GEL R Sbjct: 64 SMPLSVKEAIKAFKGEFEEGDMVVLNDPYMGGTHLPDITLVAPFFYKGELLFFLANRAHH 123 Query: 126 TDLAGEYPGGYHPEAFEMWQESMRITPVKLYSGGKLQRDVQRFVLANSRTPGXXXXXXXX 185 D+ G PG P + ++QE I P+KL G+L D + L N RTP Sbjct: 124 ADVGGATPGSM-PLSTSIFQEGFIIPPLKLMKRGELNEDFMKVFLRNVRTPEEREGDFKA 182 Query: 186 XXXXXXXXXTGLRSIIQAYGLSKVARAIGQMKAHTQEQV---LNLLPEGRKEWCGAADFS 242 LR +I+ G+ KV ++ +++ + + LPEG E+ D+ Sbjct: 183 QIMANITGIKRLRELIEREGIEKVLFFSKELMNYSERIIRERIKALPEGEYEF---EDYM 239 Query: 243 ASKVSGRVQVRMQREGNLWKA----DFTGSASQVEAPFNCSEATTSACAVVAILAEHLES 298 GR V++ + + K DF+ S Q N A T + A+++ Sbjct: 240 EDDGYGRKDVKIHLKLKVEKEKLIFDFSKSDDQTRGGINAVRAITLSAVYYAVISLLGSD 299 Query: 299 LPVNDGLLDVFDFKLPAGSIVNPAYPSATALG 330 +PVN+G + GS+V+ +P+ A G Sbjct: 300 IPVNEGCFRPLEVITRKGSVVDAEFPAGVAGG 331 >ref|NP_614331.1| N-methylhydantoinase B [Methanopyrus kandleri AV19] gb|AAM02261.1| N-methylhydantoinase B [Methanopyrus kandleri AV19] Length = 513 Score = 119 bits (299), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 88/331 (26%), Positives = 146/331 (44%), Gaps = 9/331 (2%) Query: 7 IEDQIIYGKIAAVSRHAGEQLQRISRSPLIAESRAFAAGIVTEAAGLVHQIQGEAEHLYA 66 ++ +I+ + G L+R + SP I E F+ I L+ Q + HL + Sbjct: 1 MKHEIVRQALIYACEEMGALLRRSAFSPNIREREDFSCAIYDADGELIAQAEHIPVHLGS 60 Query: 67 LRASTRAMYDHF-AYDLEEGDVLISADVYKGGTKGQMLTMVLPFFLEGELTLSPVIRVQL 125 +R + +A+ D F + ++E GD + D Y GGT LT+V P F + EL +R Sbjct: 61 MRWAVQAVLDEFGSSEMEPGDAFLLNDPYAGGTHLPDLTVVAPAFHDDELVAFAAVRAHH 120 Query: 126 TDLAGEYPGGYHPEAFEMWQESMRITPVKLYSGGKLQRDVQRFVLANSRTPGXXXXXXXX 185 D+ G+ PG +A +++ E +RI P+K+ G+ + DV R + ANSR Sbjct: 121 ADVGGKVPGSMPHDAEDVYSEGLRIPPIKVAEDGEPRDDVLRLLEANSRGGEERRYDLTA 180 Query: 186 XXXXXXXXXTGLRSIIQAYGLSKVARAIGQMKAHTQEQV---LNLLPEGRKEWCGAADFS 242 +R II +G RA K + + ++ + +P+G E+ G Sbjct: 181 QIAAAFRGCRQVRRIIDEHGREAWERACEWCKDYAERRMRVAIERIPDG--EYSGEDYLE 238 Query: 243 ASKVSG---RVQVRMQREGNLWKADFTGSASQVEAPFNCSEATTSACAVVAILAEHLESL 299 +S R+ V ++ EG+ DFTG+ Q P N T + A+ A Sbjct: 239 GDGISEDPVRISVTVRVEGDEVTVDFTGTDEQTRGPVNAPLPVTYSAVFFALKAALDPET 298 Query: 300 PVNDGLLDVFDFKLPAGSIVNPAYPSATALG 330 PV++G + P G++VNP P+ G Sbjct: 299 PVSEGTYRPIEVIAPRGTVVNPEPPAPVCAG 329 >ref|NP_343407.1| N-methyl hydantoinase B (hyuB-2) [Sulfolobus solfataricus] pir||F90367 n-methyl hydantoinase B (hyuB-2) [imported] - Sulfolobus solfataricus gb|AAK42197.1| N-methyl hydantoinase B (hyuB-2) [Sulfolobus solfataricus] Length = 510 Score = 114 bits (285), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 77/321 (23%), Positives = 137/321 (42%), Gaps = 2/321 (0%) Query: 10 QIIYGKIAAVSRHAGEQLQRISRSPLIAESRAFAAGIVTEAAGLVHQIQGEAEHLYALRA 69 +II+ ++ G L+R + SP I E + I+ ++ Q + HL + Sbjct: 5 EIIHKAFEFIAEEMGVMLKRSAMSPNIRERMDHSCAILDAEGNIIAQAEHIPVHLGSFSV 64 Query: 70 STRAMYDHFAYDLEEGDVLISADVYKGGTKGQMLTMVLPFFLEGELTLSPVIRVQLTDLA 129 + +LEEGD++I D Y GT + ++ P + G+L V + D+ Sbjct: 65 GVKNTLK--IVNLEEGDMVILNDPYISGTHLNDVMLLAPIYYNGKLIGYVVNKAHHVDVG 122 Query: 130 GEYPGGYHPEAFEMWQESMRITPVKLYSGGKLQRDVQRFVLANSRTPGXXXXXXXXXXXX 189 G PG +P A +++E + I PVK+ GKL ++ + N + P Sbjct: 123 GPSPGSLNPYATTIYEEGLVIPPVKIIKKGKLNEEIVEIIRENFKVPEVSLGDLNAQISA 182 Query: 190 XXXXXTGLRSIIQAYGLSKVARAIGQMKAHTQEQVLNLLPEGRKEWCGAADFSASKVSGR 249 ++ +I YGL +V + + + + L+ + + + D+ R Sbjct: 183 NLNGVLRVKQLIDKYGLDRVLSSWAKSIEYGRSLALSEISKWNEGSFEDEDYLELDTLKR 242 Query: 250 VQVRMQREGNLWKADFTGSASQVEAPFNCSEATTSACAVVAILAEHLESLPVNDGLLDVF 309 ++V++ ADF G+ +QVEAPFN T + + + + +P N+G V Sbjct: 243 IRVKITISDKGIIADFNGTDNQVEAPFNAVYGVTFSAVSFVVRSLIGKDIPTNEGFYSVI 302 Query: 310 DFKLPAGSIVNPAYPSATALG 330 P G+IVNP P+A G Sbjct: 303 KVIAPEGTIVNPLKPAAVGGG 323 >gb|EAJ63420.1| unknown [environmental sequence] Length = 341 Score = 113 bits (283), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 82/327 (25%), Positives = 144/327 (44%), Gaps = 10/327 (3%) Query: 10 QIIYGKIAAVSRHAGEQLQRISRSPLIAESRAFAAGIVTEAAGLVHQIQGEAE-HLYALR 68 +II + +++ L R S S I E + + IV + LV Q H+ ++R Sbjct: 14 EIISNGLRSITDECFVALTRSSYSTNIKERKDHSIAIVDKKGRLVVQAAMTIPLHIASMR 73 Query: 69 ASTRAMYDHFAYDLEEGDVLISADVYK-GGTKGQMLTMVLPFFLEGELTLSPVIRVQLTD 127 + + F D+ EGDV ++ D + GGT + +P F++GE+ D Sbjct: 74 GLMECILEKFEDDINEGDVFVANDPHTAGGTHLPDINYAMPIFIDGEIVAFSCNIAHHAD 133 Query: 128 LAGEYPGGYHPEAFEMWQESMRITPVKLYSGGKLQRDVQRFVLANSRTPGXXXXXXXXXX 187 + G PG E++QE +RI VKL+ G+LQ+D+ +L N R P Sbjct: 134 VGGMVPGSMAGGMSEIYQEGLRIPVVKLFRKGELQQDIMDILLLNVRVPNERRGDHNAQI 193 Query: 188 XXXXXXXTGLRSIIQAYGLSKVARAIGQMKAHTQEQV---LNLLPEGRKEWCGAAD---F 241 L +I+ GL V A Q+ + T ++ ++ +P G + D Sbjct: 194 AACRLGARRLLEVIEENGLKNVLSAFDQIISRTARRMRLAISEIPSGSYSFEDVMDGDGI 253 Query: 242 SASKVSGRVQVRMQREGNLWKADFTGSASQVEAPFNCSEATTSACAVVAILAEHLESLPV 301 + + + +R+ G+ DF+G++ QV N + A A A++A +P Sbjct: 254 DTTNIPICLTIRVS--GDKINLDFSGTSQQVAGNINTTFNAVQASACYALIAALDSEMPS 311 Query: 302 NDGLLDVFDFKLPAGSIVNPAYPSATA 328 N G+LDV + + G+++N +P+ A Sbjct: 312 NQGILDVVEINIEPGTLLNSVFPAPVA 338 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 706,422,453 Number of extensions: 27697521 Number of successful extensions: 58596 Number of sequences better than 1.0e-05: 187 Number of HSP's gapped: 58421 Number of HSP's successfully gapped: 187 Length of query: 506 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 376 Effective length of database: 404,274,708 Effective search space: 152007290208 Effective search space used: 152007290208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)