BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0001.p (583 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00185871.1| hypothetical protein Rxyl338101 [Rubrobacter ... 279 1e-73 ref|ZP_00185869.1| hypothetical protein Rxyl337901 [Rubrobacter ... 278 2e-73 ref|NP_745651.1| hydantoin utilization protein B [Pseudomonas pu... 267 4e-70 gb|EAI05824.1| unknown [environmental sequence] 258 3e-67 sp|Q01263|HYUB_PSESN Hydantoin utilization protein B (ORF3) >gi|... 248 4e-64 >ref|ZP_00185871.1| hypothetical protein Rxyl338101 [Rubrobacter xylanophilus DSM 9941] Length = 515 Score = 279 bits (714), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 189/529 (35%), Positives = 268/529 (50%), Gaps = 23/529 (4%) Query: 4 KVDDWLLSQIVGGTLDSVAEEMSAVVTRTARSPIFNEAHDFTTGVFDFTRSSTRLVAQAP 63 ++D LS ++ L VAEEM AV+ R A S E D + +FD + R+VAQA Sbjct: 5 RLDPVTLS-VLASALAGVAEEMGAVLIRGAYSSNIKERRDCSAALFD---ARGRMVAQAE 60 Query: 64 GCTLHLYAIVSAVDQLMEAFKNDLHPGDVLLVNDPYHGGSHSLDWTIVMPVFHGRRPILL 123 +HL A+ AV +M + D PGDV +NDPY GG+H D T+V PV + + Sbjct: 61 HIPVHLGAMPEAVAAVM---RRDPEPGDVFAINDPYSGGTHLPDITLVSPVAYEGEIVGY 117 Query: 124 PAVRSHMGDNGGPVAGGYNPRSRDIWHDGLRIPPIKLYARGEKRRDVFEMILANNRLEHW 183 R+H D GG G SR+I+ +GL IPP++L GE DV +++LAN R Sbjct: 118 AVTRAHHSDVGGMRPGSMPSDSREIYQEGLIIPPVRLVRGGEYVADVLKLLLANVRTPAL 177 Query: 184 LEGDLDAMIGACKMAAERIGQLLERYGPPSVIGAIEQRIEYTERRVREEISSWPDGTYTA 243 GDL A I A +A R+G+L+ER G +V+ A E+ I YTERR R+ I + PDG +A Sbjct: 178 RRGDLRAQIAANNIAERRVGELIERRGRETVLTAFEEVISYTERRTRDAIGALPDGERSA 237 Query: 244 ETFADHDFQGNRDIKIRATVKVEGSGLEIDFTGSSPQVAGFINSPLSNTTSFAFVAVSTC 303 E F + D + DI IRATV+VEG +EIDF+G++ VAG +N PL T S + A+ Sbjct: 238 EDFLEGDGTTDEDIPIRATVRVEGEEMEIDFSGTADAVAGNVNCPLPVTRSACYFALRVL 297 Query: 304 CDESIPINEGYMNPVKVTAPEGTVVNPRLPAPCGHATACVGAEIAEAVLLAISQCAPERA 363 + I N G P+ + APEG++VN R P+ IA+AVL A+S APE Sbjct: 298 LPKDILPNAGTYAPLTIRAPEGSLVNARSPSAVVAGNVETSNRIADAVLAALSGFAPEEI 357 Query: 364 GVNAHKLPLAYTNGTYEDGRPWVNLNXXXXXXXXXXXXETDGWGLYPPVMTGVILPSIEM 423 N GR W + G M+ + +E Sbjct: 358 PAQGQ----GTMNNLIIGGRGWTYYE--TIGGGQGASAKGPGPSGVHVGMSNTLNTPVEA 411 Query: 424 NELQYPSRVLKHEYAADYTGAGQYRGAPGLEVKIQHLEPSYTSVMMAGVRNTTRGLCGG- 482 EL+YP RV ++E GAG++RG G+ ++ LEP+ S++ R+ +G GG Sbjct: 412 FELEYPMRVERYELLYGSGGAGRHRGGDGIVRSVRVLEPASLSLLTDRRRHPPKGAAGGQ 471 Query: 483 -GDAPANLVVLGDGQSAPLEVRETAFHVHMEPGGIITFHRAGGGGWGNP 530 G NL+ DG+ P + +E G ++T GGGG+G P Sbjct: 472 PGRVGRNLI---DGEELPAKTSR-----ELEAGDVVTVETPGGGGYGRP 512 >ref|ZP_00185869.1| hypothetical protein Rxyl337901 [Rubrobacter xylanophilus DSM 9941] Length = 567 Score = 278 bits (712), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 192/533 (36%), Positives = 274/533 (51%), Gaps = 31/533 (5%) Query: 4 KVDDWLLSQIVGGTLDSVAEEMSAVVTRTARSPIFNEAHDFTTGVFDFTRSSTRLVAQAP 63 ++D LS ++ L VAEEM AV+ R A S E D + +FD + R+VAQA Sbjct: 57 RLDPVTLS-VLASALAGVAEEMGAVLIRGAYSSNIKERRDCSAALFD---ARGRMVAQAE 112 Query: 64 GCTLHLYAIVSAVDQLMEAFKNDLHPGDVLLVNDPYHGGSHSLDWTIVMPVFHGRRPILL 123 +HL A+ AV +M + D PGDV +NDPY GG+H D T+V PV + + Sbjct: 113 HIPVHLGAMPEAVAAVM---RRDPEPGDVFAINDPYSGGTHLPDITLVSPVAYEGEIVGY 169 Query: 124 PAVRSHMGDNGGPVAGGYNPRSRDIWHDGLRIPPIKLYARGEKRRDVFEMILANNRLEHW 183 R+H D GG G SR+I+ +GL IPP++L GE DV +++LAN R Sbjct: 170 AVTRAHHSDVGGMRPGSMPSDSREIYQEGLIIPPVRLVRGGEYVADVLKLLLANVRTPAL 229 Query: 184 LEGDLDAMIGACKMAAERIGQLLERYGPPSVIGAIEQRIEYTERRVREEISSWPDGTYTA 243 GDL A I A +A R+G+L+ER G +V+ A E+ I YTERR R+ I + PDG +A Sbjct: 230 RRGDLRAQIAANNIAERRVGELIERRGRETVLTAFEEVISYTERRTRDAIGALPDGERSA 289 Query: 244 ETFADHDFQGNRDIKIRATVKVEGSGLEIDFTGSSPQVAGFINSPLSNTTSFAFVAVSTC 303 E F + D + DI IRATV+VEG +EIDF+G++ VAG +N PL T S + A+ Sbjct: 290 EDFLEGDGTTDEDIPIRATVRVEGEEMEIDFSGTADAVAGNVNCPLPVTRSACYFALRVL 349 Query: 304 CDESIPINEGYMNPVKVTAPEGTVVNPRLPAPCGHATACVGAEIAEAVLLAISQCAPERA 363 + I N G P+ + APEG++VN R P+ IA+AVL A+S APE Sbjct: 350 LPKDILPNAGTYAPLTIRAPEGSLVNARSPSAVVAGNVETSNRIADAVLAALSGFAPE-- 407 Query: 364 GVNAHKLPLAYTNGTYED----GRPWVNLNXXXXXXXXXXXXETDGWGLYPPVMTGVILP 419 ++P A GT + GR W + G M+ + Sbjct: 408 -----EIP-AQGQGTMNNLIIGGRGWTYYE--TIGGGQGASAKGPGPSGVHVGMSNTLNT 459 Query: 420 SIEMNELQYPSRVLKHEYAADYTGAGQYRGAPGLEVKIQHLEPSYTSVMMAGVRNTTRGL 479 +E EL+YP RV ++E GAG++RG G+ ++ LEP+ S++ R+ +G Sbjct: 460 PVEAFELEYPMRVERYELLYGSGGAGRHRGGDGIVRSVRVLEPASLSLLTDRRRHPPKGA 519 Query: 480 CGG--GDAPANLVVLGDGQSAPLEVRETAFHVHMEPGGIITFHRAGGGGWGNP 530 GG G NL+ DG+ P + +E G ++T GGGG+G P Sbjct: 520 AGGQPGRVGRNLI---DGEELPAKTSR-----ELEAGDVVTVETPGGGGYGRP 564 >ref|NP_745651.1| hydantoin utilization protein B [Pseudomonas putida KT2440] gb|AAN69115.1| hydantoin utilization protein B [Pseudomonas putida KT2440] Length = 581 Score = 267 bits (683), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 184/575 (32%), Positives = 281/575 (48%), Gaps = 28/575 (4%) Query: 7 DWLLSQIVGGTLDSVAEEMSAVVTRTARSPIFNEAHDFTTGVFDFTRSSTRLVAQAPGCT 66 D + +V G L++ EM+ + +T RS +FN AHD++ +FD ++ Q Sbjct: 5 DPITLAVVRGALETAQREMTLTLEKTGRSSVFNLAHDYSNSLFDHL---PEMILQGQDIP 61 Query: 67 LHLYAIVSAVDQLMEAFKNDLHPGDVLLVNDPYHGGSHSLDWTIVMPVFHGRRPILLPAV 126 +HL +++ A+ + E F +D+ GDV+ NDP + GSH LD + PV++ + Sbjct: 62 IHLGSLIPAMKCVAEFFGDDIAEGDVIYHNDPAYKGSHILDCCMYKPVYYQDELVFWTVC 121 Query: 127 RSHMGDNGGPVAGGYNPRSRDIWHDGLRIPPIKLYARGEKRRDVFEMILANNRLEHWLEG 186 + H+ D GGPV GYNP +++I+ +GLRIPP+KL+ +G++R DV +L N R + EG Sbjct: 122 KGHLTDIGGPVPAGYNPDAKEIYAEGLRIPPVKLWEKGKRREDVINFMLTNMRARPYQEG 181 Query: 187 DLDAMIGACKMAAERIGQLLERYGPPSVIGAIEQRIEYTERRVREEISSWPDGTYTAETF 246 DL+A GACK+ + +LL++YG V I + +R +R + PDG Y+ Sbjct: 182 DLNAQYGACKVGERHLLELLDKYGVAQVRACIAELKNMADRHMRALLRDVPDGDYSGTAV 241 Query: 247 ADHDFQGNRDIKIRATVKVEGSGLEIDFTGSSPQVAGFINSPLSNTTSFAFVAVSTCCDE 306 + G ++ I A V++ G + S PQV FINS N+ S ++ + Sbjct: 242 LEDAGHGLGELAITAHVQIRGDQAHVRIE-SPPQVPYFINSYEGNSVSGVYLGLMMFAQV 300 Query: 307 SIPINEGYMNPVKV-TAPEGTVVNPRLPAPCGHATACVGAEIAEAVLLAISQCAPER--- 362 + P NEG V V P GT+ N PAP + T +A+AV A+ Q +P+R Sbjct: 301 APPYNEGLYRCVTVDVGPRGTLCNAEEPAPHVNCTTTPMETLADAVRTALEQASPQRVTA 360 Query: 363 -----AGVN-AHKLPLAYTNGTYEDGRPWVNLNXXXXXXXXXXXXETDGWGLYPPVMTGV 416 +G+N A + P NG E +V + DGW P+ Sbjct: 361 SWGHSSGINIAGRDP---RNGNSE----YVTMVLASIISGAGANKVMDGWHACGPLCCFG 413 Query: 417 ILPS--IEMNELQYPSRVLKHEYAADYTGAGQYRGAPGLEVKIQHLEPSYTSVMMAGVRN 474 L S IE+ E YP + ++ AD GAG+ RG G ++I+ L+ + T V R Sbjct: 414 ALMSGDIELLEHAYPVLIHRYSLMADSGGAGELRGGSGTRLEIEPLDHAMTVVGFGEGRQ 473 Query: 475 TTRGLCGGGDAPANLVVLGDGQSAPLEVRETAF----HVHMEPGGIITFHRAGGGGWGNP 530 G LG + RE + + ++PG I GGGG+G+P Sbjct: 474 LPTAGAAGARNALLEPKLGRLIHRHADGREDHYTQNPMLTLQPGERIININPGGGGYGDP 533 Query: 531 LLREPGKVLEDVLNGIVSVEGAEREYGVGIVRVDG 565 L R VLEDV NG+VS +GA EYGV ++ DG Sbjct: 534 LRRRVAAVLEDVRNGLVSPQGAALEYGV-VLDADG 567 >gb|EAI05824.1| unknown [environmental sequence] Length = 591 Score = 258 bits (659), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 174/577 (30%), Positives = 287/577 (49%), Gaps = 40/577 (6%) Query: 7 DWLLSQIVGGTLDSVAEEMSAVVTRTARSPIFNEAHDFTTGVFDFTRSSTRLVAQAPGCT 66 D + +++ + ++ M++V+ R++ SPI + DF+ ++D + +L++QA C Sbjct: 15 DPITFEVMRSIFEFASDRMASVLQRSSFSPILADMLDFSNAIYD---ADLQLLSQAANCP 71 Query: 67 LHLYAIVSAVDQLMEAF-KNDLHPGDVLLVNDPYHGGSHSLDWTIVMPVFHGRRPILLPA 125 +HL A+ + ++ ++ K+++H GDVL+VNDPY GG+H D + + P+++ + I Sbjct: 72 VHLAAMKFSAEEAVKGIGKDNVHEGDVLVVNDPYRGGTHINDISFIKPIYYKKELIGYGV 131 Query: 126 VRSHMGDNGGPVAGGYNPRSRDIWHDGLRIPPIKLYARGEKRRDVFEMILANNRLEHWLE 185 R H D GG G S I +GLR+PP+K++ G D+ ++L N+R H+++ Sbjct: 132 SRGHWMDLGG-GGAGGQAFSTHIAAEGLRLPPLKIFTDGRINEDLLAIMLNNSRTPHFIK 190 Query: 186 GDLDAMIGACKMAAERIGQLLERYGPPSVIGAIEQRIEYTERRVREEISSWPDGTYTAET 245 GDL A +GA + A + + + +RYG +V A+++ YTE+ +RE I P+G Y AE Sbjct: 191 GDLQAHLGALRGAEQELQRACDRYGVDTVKKAMKKIQAYTEKMIRESIGKIPNGVYEAED 250 Query: 246 FADHDFQGNRDIKIRATVKVEGSGLEIDFTGSSPQVAGFINSPLSNTTSFAFVAVSTCCD 305 +AD D + +KI+ +++ + +++DFTG+ P G INSP +NT S A ++ Sbjct: 251 YADTDGVSHERVKIKVKLEIRDTSIKVDFTGTDPICQGAINSPRANTISAALYSLQFFLA 310 Query: 306 ESIPINEGYMNPVKVTAPEGTVVNPRLPAPCGHATACVGAEIAEAVLLAISQCAPER-AG 364 P N+G NP++V PEG +N + PAP T ++I A+ A+++ PE+ G Sbjct: 311 PDAPQNQGMFNPIEVVLPEGCWLNAQWPAPTIGCTTLTSSKITSAIWQALAKAIPEKITG 370 Query: 365 VNAHKLPLAYTNGTYEDGRPWVNLNXXXXXXXXXXXXETDGWGLYP----------PVMT 414 + + T DG+ V GWG P P+ Sbjct: 371 STCAECNWFVASCTSPDGKTDV-----------FSDLPAGGWGGTPYADGMSVTMDPLGN 419 Query: 415 GVILPSIEMNELQYPSRVLKHEYAADYTGAGQYRGAPGLEVKIQHLEPSYTSVMMAGVRN 474 + +P+ E EL +P E D G GQ+RG G K++ L + A Sbjct: 420 CMNMPA-ETAELFFPIAYEAFEMRQDSAGPGQHRGGLGAVFKVRFLGGGELGMETARTLE 478 Query: 475 TTRGLCGGG----------DAPANLVVLGDGQSAPLEVRETAFHVH-MEPGGIITFHRAG 523 + G GG A ++ V+G G A H G + F G Sbjct: 479 GSPGAAGGLPSAVQRSSHIRADGSVQVIG-GIDAEGAWSNPLLSGHKFRAGEVFMFESTG 537 Query: 524 GGGWGNPLLREPGKVLEDVLNGIVSVEGAEREYGVGI 560 GGGWGNP R +VL+DVL+ +S+E AE+ YGV I Sbjct: 538 GGGWGNPKRRTVEEVLDDVLDEYISIEAAEKHYGVVI 574 >sp|Q01263|HYUB_PSESN Hydantoin utilization protein B (ORF3) pir||C42594 L-amino acid hydantoin hydrolase (ATP-hydrolyzing) (EC 3.5.2.-) hyuB [validated] - Pseudomonas sp. plasmid pHN671 gb|AAA25846.1| DL-hydantoinase dbj|BAA01378.1| hydantoinase [Pseudomonas sp.] Length = 592 Score = 248 bits (632), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 182/590 (30%), Positives = 282/590 (47%), Gaps = 25/590 (4%) Query: 7 DWLLSQIVGGTLDSVAEEMSAVVTRTARSPIFNEAHDFTTGVFDFTRSSTRLVAQAPGCT 66 D + Q+V G+L++VA EM + R + S I E+ DF + D +L + Sbjct: 12 DPITVQVVLGSLENVAVEMGHKLARMSYSSIIRESEDFGCALVDV--RGQQLCESSHSTP 69 Query: 67 LHLYAI---VSAVDQLMEAFKNDLHPGDVLLVNDPYHGGSHSLDWTIVMPVFHGRRPILL 123 L I + + ++ME + + GDV++ N PYHG SH D +PVF+ I Sbjct: 70 LQSGPIPGYIKGIREIMEDRNDTFNQGDVIMHNSPYHGASHGPDVGFCIPVFYKDELIGF 129 Query: 124 PAVRSHMGDNGGPVAGGYN-PRSRDIWHDGLRIPPIKLYARGEKRRDVFEMILANNRLEH 182 +H D G G + D + +GL+ IK+Y +G K R V++++ N R Sbjct: 130 SVTTAHHLDIGSSTPGSCGIVDAVDAYAEGLQFKAIKVYDQGVKNRYVWDILKDNIRAPK 189 Query: 183 WLEGDLDAMIGACKMAAERIGQLLERYGPPSVIGAIEQRIEYTERRVREEISSWPDGTYT 242 + GD++A I A ++ A+R +++E+YG +V A E+ + Y+E+ +R+ I PDG YT Sbjct: 190 LVVGDMEAQIAAARIGAQRYIEIIEKYGLDTVQAASEELMNYSEKMMRDAIKKLPDGEYT 249 Query: 243 AETF----ADHDFQGNRDIKIRATVKVEGSGLEIDFTGSSPQVAGF-INSPLSNTTSFAF 297 AE F D D +D++I TVKV+GS L +D TG+SPQV IN PL T A Sbjct: 250 AEGFLDGYLDSDDPAKKDLRINVTVKVDGSDLTVDLTGTSPQVTDKPINMPLLGTVDIAI 309 Query: 298 VAV--STCCDESI----PINEGYMNPVKVTAPEGTVVNPRLPAPCGHATACVGAEIAEAV 351 S D ++ P N G + P+K+ AP+GT+ NP PAP A G +A+ + Sbjct: 310 YLTLRSILLDSTVYGNFPQNSGLIRPIKIVAPKGTLCNPIFPAPT-IARFNSGNAVADTL 368 Query: 352 LLAISQCAPERAGVNAHKLPLAYTNGTYEDGRPWVNLNXXXXXXXXXXXXETDGWGLYPP 411 + A++Q P + L + +G + WV ++ DG Sbjct: 369 MKALAQVVPHQVSAGVGNLQVVAFSGQSNENY-WVYMDIMEGSYGGRYG--KDGMDAVDT 425 Query: 412 VMTGVILPSIEMNELQYPSRVLKHEYAADYTGAGQYRGAPGLEVKIQHLEPSYTSVMMAG 471 + IE E YP RV ++E + + G++RG G ++ L SV G Sbjct: 426 LYANTRNNPIEDIESHYPLRVNRYELRDNDSAPGKWRGGIGSIREVSFLADGSFSVEADG 485 Query: 472 VRNTTRGLCGGGDAPANLVVLGDGQSAPL-EVRETAFHVHMEPGGIITFHRAGGGGWGNP 530 + G G D + + D ++ L ++ + H + G I GGG+GNP Sbjct: 486 HKYAPWGFDDGQDGYVGSLSIRDNETNELVQLPSKLPNRHAQSGSTIQLVGPCGGGYGNP 545 Query: 531 LLREPGKVLEDVLNGIVSVEGAEREYGVGIVRVDGGFAVDDRKTAELRQL 580 L REP KVL D L+G ++ E A EYGV I + +D KT ELR++ Sbjct: 546 LEREPEKVLSDYLDGFITKEKALVEYGVTITDSE---EIDYEKTNELRKV 592 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 918,915,756 Number of extensions: 41473085 Number of successful extensions: 88051 Number of sequences better than 1.0e-05: 362 Number of HSP's gapped: 87318 Number of HSP's successfully gapped: 379 Length of query: 583 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 452 Effective length of database: 401,559,609 Effective search space: 181504943268 Effective search space used: 181504943268 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)