BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0031.p (389 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_346844.1| Hydrolase of the alpha/beta superfamily [Clostr... 454 e-126 ref|NP_350022.1| Alpha/beta superfamily hydrolase [Clostridium a... 180 5e-44 ref|ZP_00062904.1| COG0596: Predicted hydrolases or acyltransfer... 177 4e-43 ref|ZP_00063163.1| COG0596: Predicted hydrolases or acyltransfer... 177 5e-43 ref|NP_721654.1| conserved hypothetical protein [Streptococcus m... 151 2e-35 >ref|NP_346844.1| Hydrolase of the alpha/beta superfamily [Clostridium acetobutylicum] pir||E96924 hydrolase of the alpha/beta superfamily [imported] - Clostridium acetobutylicum gb|AAK78184.1| Hydrolase of the alpha/beta superfamily [Clostridium acetobutylicum] Length = 388 Score = 454 bits (1167), Expect = e-126, Method: Compositional matrix adjust. Identities = 216/378 (57%), Positives = 281/378 (74%), Gaps = 5/378 (1%) Query: 11 FPVGFYEELHPDFSINFQMNRFYSWTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQQAV 70 FPVGFY+++HPDFS+NFQMNR Y+ TND ML+EMR +P IHNY E IE L+L +A+ Sbjct: 11 FPVGFYDDIHPDFSVNFQMNRCYTMTNDDVMLKEMREISPSIHNYEEFIEKFLELYNKAL 70 Query: 71 AADKKLKAANYLRGAEFYMPEGHPLKHTSRQQFIVLTRAHYQVEDNQHFDIPYGKGFLSA 130 KL+AA YLR AEFYM HP K R+QFI L + ++ + D Q + +PY GFL A Sbjct: 71 NEGHKLRAAYYLRTAEFYMTADHPQKQNYRKQFISLMQEYFNISDEQPYKVPYENGFLPA 130 Query: 131 YRFSPEVPAKGSIVLFGGFDSYIEEIFLMALVFKDAGYDVVCFDGPGQGAALEDWQLPMT 190 YRF+ E P KG+IV+FGGFDSY+EE+F MAL K+AGYDVV FDGPGQG+ALED+++PMT Sbjct: 131 YRFTSEAP-KGTIVMFGGFDSYVEEMFKMALFLKEAGYDVVFFDGPGQGSALEDYKIPMT 189 Query: 191 HEWEKPVATVLDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADDICTDFYKVLLR 250 HEWEKPV ++LD+F L +VTL+ +E RV RV+ADDI ++FY+V+L Sbjct: 190 HEWEKPVKSILDYFELDDVTLLGISLGGYLTVRAAAFE-KRVHRVIADDILSNFYEVVL- 247 Query: 251 QTGP--LEDSISTLMAKENEAEINALFSRLMKENLMLEWGITQGMHVTGSQTPYGFLKQS 308 T P L++ +++LM+ NE E+N LF LMK++LM+EW I QGMH+TGS+TPY FL + Sbjct: 248 NTLPSQLKEKVNSLMSDGNEQEVNILFKDLMKKSLMVEWAINQGMHITGSKTPYEFLNKI 307 Query: 309 MLYTTAGISPLLSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLFTRKEQAQNHC 368 +LY T+ IS L QDVLL+AAQEDHYIPL QF +Q+ SL +VRSLTTR+FTRKE AQNHC Sbjct: 308 LLYNTSKISEKLEQDVLLMAAQEDHYIPLNQFFDQSKSLINVRSLTTRMFTRKETAQNHC 367 Query: 369 HVGNIGLSIHVMLHWLQQ 386 GN+GL+ V+ +W++Q Sbjct: 368 QSGNLGLAFEVIANWIEQ 385 >ref|NP_350022.1| Alpha/beta superfamily hydrolase [Clostridium acetobutylicum] pir||G97321 alpha/beta superfamily hydrolase [imported] - Clostridium acetobutylicum gb|AAK81362.1| Alpha/beta superfamily hydrolase [Clostridium acetobutylicum] Length = 371 Score = 180 bits (456), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 111/360 (30%), Positives = 179/360 (49%), Gaps = 5/360 (1%) Query: 26 NFQMNRFY-SWTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQQAVAADKKLKAANYLRG 84 NFQ+NRF + D E+ ++ A I + KL +++ K A+ Y Sbjct: 13 NFQLNRFLGKFMEDSEVKNDIEKALLNIKDMNSWYIAWRKLAEKSEIKGKFELASAYYAA 72 Query: 85 AEFYMPEGHPLKHTSRQQFIVLTRAHYQVEDNQHFDIPYGKGFLSAYRFSPEVPAKGSIV 144 FY+ E P K ++ Y+ +HF++PY F A R + K +++ Sbjct: 73 GSFYLRESDPNKAYMYTKYRENYYKSYKGVPLEHFNVPYEDSFFPAVRVKFQGSTK-TLI 131 Query: 145 LFGGFDSYIEEIFLMALVFKDAGYDVVCFDGPGQGAALEDWQLPMTHEWEKPVATVLDFF 204 GG+DS +EE+ F++ GYD++ F+GPGQG AL++ L H WE+PV+ +LD+F Sbjct: 132 FHGGYDSCLEEMLPFFSAFENLGYDIIAFEGPGQGMALKN-GLKFIHNWERPVSALLDYF 190 Query: 205 NLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADDICTDFYKVLLRQTGPLEDSISTLMA 264 NL EVT++ +E R+K + DI V+ + + + L+ Sbjct: 191 NLNEVTILGCSWGGYFCLRAAAFEK-RIKAAIVYDIFYCGMDVIGMKNKEMRYELEKLLN 249 Query: 265 KENEAEINALFSRLMKENLMLEWGITQGMHVTGSQTPYGFLKQSMLYTTAGISPLLSQDV 324 + +IN L MK ++ W I++GM T + TPY FLK L+T GI L+ QDV Sbjct: 250 NNEKDKINELMYSKMKADIDFNWKISKGMDNTMTYTPYDFLKAIQLHTMDGIEELIDQDV 309 Query: 325 LLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLFTRKEQAQNHCHVGNIGLSIHVMLHWL 384 LLLA +ED Y+P+ L + ++T ++FT++ + HC VG I L+ + +L Sbjct: 310 LLLAGEEDQYVPIEAMKLFEDRLVNA-NITKKIFTKETGGEQHCQVGRIDLAFDEIKKFL 368 >ref|ZP_00062904.1| COG0596: Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293] Length = 389 Score = 177 bits (449), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 109/373 (29%), Positives = 184/373 (49%), Gaps = 5/373 (1%) Query: 15 FYEELHPDFSINFQMNR-FYSWTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQQAVAAD 73 FYE + + N Q+NR ++ ++++ P + N A + G+ + Sbjct: 13 FYEYIKNNPQWNLQINRQLEGIKESSKVKDDLKIIIPKLTNDASWYKTWFSFGELREKEE 72 Query: 74 KKLKAANYLRGAEFYMPEGHPLKHTSRQQFIV-LTRAHYQVEDNQHFDIPYGKGFLSAYR 132 A Y A+FY+ + K T +++ RAH ++ D + + +PY K FL A + Sbjct: 73 DHALACAYFGLAQFYLNQDDENKQTVVHRYLNNFYRAHTEI-DYESYQVPYQKTFLPAIK 131 Query: 133 FSPEVPAKGSIVLFGGFDSYIEEIFLMALVFKDAGYDVVCFDGPGQGAALEDWQLPMTHE 192 + A ++++ GFDS++EEI + FK Y+V+ FDGPGQG AL D + T Sbjct: 132 VNINPTATKTLIIINGFDSFMEEILVAVNFFKGTDYNVILFDGPGQGRALIDNNIKFTPY 191 Query: 193 WEKPVATVLDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADDICTDFYKVLLRQ- 251 +E PV+ ++D+FNL EV + YE R+K VV D D L+ Sbjct: 192 FENPVSAIIDYFNLDEVDAMGVSWGGYFVIRAAAYEK-RIKNVVCYDFFYDGLNTFLKDF 250 Query: 252 TGPLEDSISTLMAKENEAEINALFSRLMKENLMLEWGITQGMHVTGSQTPYGFLKQSMLY 311 + + I L+ + + N L L+ + L + T+G TG++TPY LK+ + Sbjct: 251 SKDTRNHIRQLLQNNDVEKTNQLIPTLIHSDANLNFLFTKGYENTGTETPYSLLKEIERH 310 Query: 312 TTAGISPLLSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLFTRKEQAQNHCHVG 371 T GI L++Q+VLLLA ++D Y+P + LT+ +SL T++FT++ + HC G Sbjct: 311 TVKGIGHLVNQNVLLLAGKDDQYVPFNDLVLEQQELTNAKSLETKIFTKETGGEQHCQAG 370 Query: 372 NIGLSIHVMLHWL 384 L++ + +L Sbjct: 371 RYDLALVAIQKFL 383 >ref|ZP_00063163.1| COG0596: Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293] Length = 401 Score = 177 bits (448), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 106/366 (28%), Positives = 181/366 (49%), Gaps = 14/366 (3%) Query: 26 NFQMNRFYS--WTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQQAVAADKKLK---AAN 80 N Q+NRF + + ND ++ E++++ P + + + +A+A ++ A+ Sbjct: 40 NLQINRFINDEYENDQKVQEDLKSIIPQLKDEESWFKAW---NDKAIAREQDGDFSVAST 96 Query: 81 YLRGAEFYMPEGHPLKHTSRQQFIVLTRAHYQVEDNQHFDIPYGKGFLSAYRF-SPEVPA 139 Y + +EFY+ P + +++ + + + + +PY G+L + +P A Sbjct: 97 YYQASEFYLSPEDPRDENAYRKYRENFYKGFNDFEYERYQVPYENGYLPIVKLVTPN--A 154 Query: 140 KGSIVLFGGFDSYIEEIFLMALVFKDAGYDVVCFDGPGQGAALEDWQLPMTHEWEKPVAT 199 +++LF G+DSY+EE+ M+ K Y++ FDGPGQG AL + + H WE PV+ Sbjct: 155 TKNLLLFAGYDSYMEEMVKMSKFLKGIDYNIYVFDGPGQGTALRQG-IHLIHNWENPVSV 213 Query: 200 VLDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADDICTDFYKVL-LRQTGPLEDS 258 +LD+FNL+ + + +E R+ ++VA DI F+ L +R Sbjct: 214 ILDYFNLKRASAVGMSLGGYLVMRAAAFEK-RLDKIVAFDIFYSFFDALRIRMPYNQITK 272 Query: 259 ISTLMAKENEAEINALFSRLMKENLMLEWGITQGMHVTGSQTPYGFLKQSMLYTTAGISP 318 I L+A ++ +N +F +M NL L W I +GM G Q+PY LKQ Y I P Sbjct: 273 IEQLLAAKDAKTVNFMFKSMMANNLDLNWKINKGMANVGVQSPYELLKQFQKYNMEPIMP 332 Query: 319 LLSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLFTRKEQAQNHCHVGNIGLSIH 378 L++QDVLLLA + D Y+P + E L + S+ + +F + HC GN L Sbjct: 333 LINQDVLLLAGENDQYVPSSRLVEIDSKLINAASVKSIMFDKASGGDQHCQAGNRQLGFD 392 Query: 379 VMLHWL 384 + +L Sbjct: 393 AIKEFL 398 >ref|NP_721654.1| conserved hypothetical protein [Streptococcus mutans UA159] gb|AAN58960.1| conserved hypothetical protein [Streptococcus mutans UA159] Length = 372 Score = 151 bits (382), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 99/353 (28%), Positives = 182/353 (51%), Gaps = 11/353 (3%) Query: 26 NFQMNRF-YSWTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQQAVAADKKLKAANYLRG 84 + Q+NR + + + ++ E+++ P + + + ++ + A AA+Y G Sbjct: 12 DLQINRLCFPFEGNEKVEEDLKLIVPQLKDISSWNKVWKEFALMREAKGDYALAASYFFG 71 Query: 85 AEFYMPEGHPLKHTSRQQFIV-LTRAHYQVEDNQHFDIPYGKGFLSAYRFSPEVPAKGSI 143 A FY+ E + ++ + RA+ + +H +IPY L A F + AK ++ Sbjct: 72 ASFYLLEDDTDRELIQEHRLQNFYRAYGDLGFERH-EIPYDNTSLPAVTFM-QPSAKKNL 129 Query: 144 VLFGGFDSYIEEIFLMALVFKDAGYDVVCFDGPGQGAALEDWQLPMTH---EWEKPVATV 200 ++FGG+DS +EE+ L ++ GY+++ FDGPGQG L ++ +H +EKPVA V Sbjct: 130 LIFGGYDSNLEELILWFSPLRNNGYNLIIFDGPGQGNML--FKKEKSHFIMNFEKPVAAV 187 Query: 201 LDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADDICTDFYKVLLRQTGPLEDSI- 259 LD+F+L V + +E R+++++A DI L P+ I Sbjct: 188 LDYFSLDRVAALGLSWGGFFVMRAAAFE-KRIEKIIALDIFYQGLDALTIAMNPVLAFIF 246 Query: 260 STLMAKENEAEINALFSRLMKENLMLEWGITQGMHVTGSQTPYGFLKQSMLYTTAGISPL 319 TL+ + + I+ L + M +L L W I +G +TG++TPY ++ + G+ PL Sbjct: 247 KTLVNFKCKTWIDVLLTHAMTRDLDLNWKIKRGYQLTGTKTPYDLIQNIKKHNMKGLGPL 306 Query: 320 LSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLFTRKEQAQNHCHVGN 372 ++Q+VLLLA ++D Y+P+ + G+ L++ + T LFT++ + HC G+ Sbjct: 307 INQEVLLLAGEKDQYVPVKRLGQIKKELSNAAEIKTHLFTQESGGEQHCQAGS 359 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 564,732,988 Number of extensions: 22311944 Number of successful extensions: 43644 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 43622 Number of HSP's successfully gapped: 15 Length of query: 389 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 262 Effective length of database: 412,420,005 Effective search space: 108054041310 Effective search space used: 108054041310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)