BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0010.p (718 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAB18278.1| heparinase III protein 154 1e-35 pir||JC4910 heparin-sulfate lyase (EC 4.2.2.8) - Flavobacterium ... 147 1e-33 ref|NP_813568.1| heparinase III protein [Bacteroides thetaiotaom... 142 2e-32 ref|NP_736319.1| Unknown [Streptococcus agalactiae NEM316] >gi|2... 116 2e-24 ref|NP_688886.1| conserved hypothetical protein [Streptococcus a... 115 3e-24 >gb|AAB18278.1| heparinase III protein Length = 659 Score = 154 bits (388), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 131/550 (23%), Positives = 221/550 (40%), Gaps = 63/550 (11%) Query: 105 WNSWLYDSSQYQLHLTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDNPMPVDGTFR 164 W W ++ + L R + + +A Y TGDEKYA+ + S + NP+ + Sbjct: 124 WQMWPVKDNEVRWQLHRVKWWQAMALVYHATGDEKYAREWVYQYSDWARKNPLGLSQD-- 181 Query: 165 AEHSTWDPLSVGVRLFMLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVRKYHATHGNH 224 + W PL V R+ LP F F +SP+F P ++ + S+H+ Y+ ++A GNH Sbjct: 182 NDKFVWRPLEVSDRVQSLPPTFSLFVNSPAFTPAFLMEFLNSYHQQADYLSTHYAEQGNH 241 Query: 225 VCMQLRGLIQTALLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFEGSPNYHLVVMRD 284 + + + + PE KDS W + G+ + I++ VY DG+QFE SP YH+ + Sbjct: 242 RLFEAQRNLFAGVSFPEFKDSPRWRQTGISVLNTEIKKQVYADGMQFELSPIYHVAAIDI 301 Query: 285 LYELVALFHKLGIAA---GEYQEILENMFVVMMHLLAPDGQLVKFGD---TDQ-----QV 333 + ++ + Y + +ENM + ++ + PD FGD TD+ Q Sbjct: 302 FLKAYGSAKRVNLEKEFPQSYVQTVENMIMALISISLPDYNTPMFGDSWITDKNFRMAQF 361 Query: 334 ASELRNVMSLGAYLYQRSDFKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAACFP 393 AS R + A Y +D K + P + Sbjct: 362 ASWARVFPANQAIKYFATDGK--------------------------QGKAPNFLSKALS 395 Query: 394 AGGYLTSRQGWKHTDMYMAMRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFEWD-- 451 G+ T R GW M ++A HA D + L+ GR D+G+ ++ D Sbjct: 396 NAGFYTFRSGWDKNATVMVLKASPPGEFHAQPDNGTFELFIKGRNFTPDAGVFVYSGDEA 455 Query: 452 --KERKYVVSTRAHNTVVVDGQDQHVRSFHWNTPATAACRIWDFQTNEQYDYTFASHYGY 509 K R + TR H+T+ +D Q+ + R ++T D ++ Y Sbjct: 456 IMKLRNWYRQTRIHSTLTLDNQNMVI----------TKARQNKWETGNNLDVLTYTNPSY 505 Query: 510 TRYDDPVIHSRKVLFVKNSYWLIVDLFEAKGQHRYEQYFHLPP-GGVAYDCFTGEVSTQL 568 D H R VLF+ Y+L++D + ++ L +D V T Sbjct: 506 PNLD----HQRSVLFINKKYFLVIDRAIGEATGNLGVHWQLKEDSNPVFDKTKNRVYTTY 561 Query: 569 EDANLLLVHPSAGQEMDQLSVEPGLV-FKQGNYYDHP--VVKRSLTAAGRTVIATLAVPF 625 D N L++ S + L+ E G V + P V ++ AG ++ P+ Sbjct: 562 RDGNNLMIQ-SLNADRTSLNEEEGKVSYVYNKELKRPAFVFEKPKKNAGTQNFVSIVYPY 620 Query: 626 -GTAKPKVKV 634 G P++ + Sbjct: 621 DGQKAPEISI 630 >pir||JC4910 heparin-sulfate lyase (EC 4.2.2.8) - Flavobacterium heparinum Length = 659 Score = 147 bits (370), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 129/550 (23%), Positives = 218/550 (39%), Gaps = 63/550 (11%) Query: 105 WNSWLYDSSQYQLHLTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDNPMPVDGTFR 164 W W ++ + L R + + TGDEKYA+ + S + NP+ + Sbjct: 124 WQMWPVKDNEVRWQLHRVKWWQLWPWFITATGDEKYAREWVYQYSDWARKNPLGLSQD-- 181 Query: 165 AEHSTWDPLSVGVRLFMLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVRKYHATHGNH 224 + W PL V R+ LP F F +SP+F P ++ + S+H+ Y+ ++A GNH Sbjct: 182 NDKFVWRPLEVSDRVQSLPPTFSLFVNSPAFTPAFLMEFLNSYHQQADYLSTHYAEQGNH 241 Query: 225 VCMQLRGLIQTALLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFEGSPNYHLVVMRD 284 + + + + PE KDS W + G+ + I++ VY DG+QFE SP YH+ + Sbjct: 242 RLFEAQRNLFAGVSFPEFKDSPRWRQTGISVLNTEIKKQVYADGMQFELSPIYHVAAIDI 301 Query: 285 LYELVALFHKLGIAA---GEYQEILENMFVVMMHLLAPDGQLVKFGD---TDQ-----QV 333 + ++ + Y + +ENM + ++ + PD FGD TD+ Q Sbjct: 302 FLKAYGSAKRVTLEKEFPQSYVQTVENMIMALISISLPDYNTPMFGDSWITDKNFRMAQF 361 Query: 334 ASELRNVMSLGAYLYQRSDFKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAACFP 393 AS R + A Y +D K + P + Sbjct: 362 ASWARVFPANQAIKYFATDGK--------------------------QGKAPNFLSKALS 395 Query: 394 AGGYLTSRQGWKHTDMYMAMRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFEWD-- 451 G+ T R GW M ++A HA D + L+ GR D+G+ ++ D Sbjct: 396 NAGFYTFRSGWDKNATVMVLKASPPGEFHAQPDNGTFELFIKGRNFTPDAGVFVYSGDEA 455 Query: 452 --KERKYVVSTRAHNTVVVDGQDQHVRSFHWNTPATAACRIWDFQTNEQYDYTFASHYGY 509 K R + TR H+T+ +D Q+ + R ++T D ++ Y Sbjct: 456 IMKLRNWYRQTRIHSTLTLDNQNMVI----------TKARQNKWETGNNLDVLTYTNPSY 505 Query: 510 TRYDDPVIHSRKVLFVKNSYWLIVDLFEAKGQHRYEQYFHLPP-GGVAYDCFTGEVSTQL 568 D H R VLF+ Y+L++D + ++ L +D V T Sbjct: 506 PNLD----HQRSVLFINKKYFLVIDRAIGEATGNLGVHWQLKEDSNPVFDKTKNRVYTTY 561 Query: 569 EDANLLLVHPSAGQEMDQLSVEPGLV-FKQGNYYDHP--VVKRSLTAAGRTVIATLAVPF 625 D N L++ S + L+ E G V + P V ++ AG ++ P+ Sbjct: 562 RDGNNLMIQ-SLNADRTSLNEEEGKVSYVYNKELKRPAFVFEKPKKNAGTQNFVSIVYPY 620 Query: 626 -GTAKPKVKV 634 G P++ + Sbjct: 621 DGQKAPEISI 630 >ref|NP_813568.1| heparinase III protein [Bacteroides thetaiotaomicron VPI-5482] gb|AAO79762.1| heparinase III protein [Bacteroides thetaiotaomicron VPI-5482] Length = 666 Score = 142 bits (359), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 145/614 (23%), Positives = 237/614 (38%), Gaps = 84/614 (13%) Query: 9 TMLDLDRPELAEAAAKLHEGNVSGARDAYLEVIASGNTGRYYFDVRDVPGLKAYAVNSYS 68 ++L+LD P L + A EG A A L+ + R D+ LK + Sbjct: 31 SLLNLDYPGLEKVKALHQEGKDEDAAKALLDYYRA----RTNVKTPDI-NLKKITI---- 81 Query: 69 QEEEAKQDIAEADRIAEGDIPLFKGKRVVFPKGT-YEWNSWLYDSSQYQLHLTRFVYVKR 127 K++ AD + + KG + + G W W ++ + L R + Sbjct: 82 ----GKEEQQWADDGLKHTFFVHKGYQPSYNYGEDINWQYWPVKDNELRWQLHRHKWFTP 137 Query: 128 LARAYALTGDEKYAKCFNDMMSHFIDDNPMP----------VDGTFRAE----HSTWDPL 173 + +AY ++GDEKYAK + +I NP+ DG + E W PL Sbjct: 138 MGKAYRVSGDEKYAKEWAYQYIDWIKKNPLVKMDKKEYELVSDGKIKGEVENVRFAWRPL 197 Query: 174 SVGVRLFMLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVRKYHATHGNHVCMQLRGLI 233 V RL F F SPSF P+ + + ++H+H ++ ++ GNH+ + + +I Sbjct: 198 EVSNRLQDQTTQFQLFLPSPSFTPDFLTEFLVNYHKHAVHILANYSDQGNHLLFEAQRMI 257 Query: 234 QTALLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFEGSPNYHLVVMRDLYELVALFH 293 PE K++ W + G+ + + VY DG QFE P+YHL + +F Sbjct: 258 YAGAFFPEFKEAPAWRKSGIDILNREVNVQVYNDGGQFELDPHYHLAA-------INIFC 310 Query: 294 K-LGIA---------AGEYQEILENMFVVMMHLLAPDGQLVKFGDTDQQVASE-LRNVMS 342 K LGIA EY + +E M + ++ PD F D E L+N + Sbjct: 311 KALGIADVNGFRNEFPQEYLDTIEKMIMFYANISFPDYTNPCFSDAKITEKKEMLKNYRA 370 Query: 343 LGAYLYQRSDFKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAACFPAGGYLTSRQ 402 + K L + +L P + F G+ R Sbjct: 371 WSKLFPKNETIKYLATDGKEGAL-------------------PDYMSKGFLKSGFFVFRN 411 Query: 403 GWKHTDMYMAMRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLF----EWDKERKYVV 458 W M ++AG H D + ++ G+ L DSG ++ E ++R + Sbjct: 412 SWGMDATQMVVKAGPKGFWHCQPDNGTFEMWFNGKNLFPDSGSYVYAGEGEVMEQRNWHR 471 Query: 459 STRAHNTVVVDGQDQHVRSFHWNTPATAACRIWDFQTNEQYDYTFASHYGYTRYDDPVIH 518 T HNTV +D ++ + ++W + N Q T Y + H Sbjct: 472 QTSVHNTVTLDNKNLET--------TESVTKLWQPEGNIQTLVTENPSYKNFK------H 517 Query: 519 SRKVLFVKNSYWLIVDLFEAKGQHRYEQYFHLPPGGVAYDCFTGEVSTQLEDANLLLVHP 578 R V FV N+Y++IVD + ++ +P G +A TQ ED + + + Sbjct: 518 RRSVFFVDNTYFVIVDEVSGSAKGSVNLHYQMPKGEIANSREDMTFLTQFEDGSNMKLQ- 576 Query: 579 SAGQEMDQLSVEPG 592 G E + EPG Sbjct: 577 CFGPEGMSMKKEPG 590 >ref|NP_736319.1| Unknown [Streptococcus agalactiae NEM316] emb|CAD47544.1| Unknown [Streptococcus agalactiae NEM316] Length = 634 Score = 116 bits (290), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 132/611 (21%), Positives = 244/611 (39%), Gaps = 59/611 (9%) Query: 59 LKAYAVNSYSQEEEAKQDIAEADRIAEGDIPLFKGKRVVFPKGTYEWNSWLYDSSQYQLH 118 +K Y N+Y QE +A ++ + D + + EW+ + D ++ Sbjct: 21 IKTYQSNAY-QEMKASVNLMMRNTFVFNDNWDMEPCSKAYCLDPLEWDKPVTDDPEWLYM 79 Query: 119 LTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDNPMPVDGTFRAEHSTWDPLSVGVR 178 L R Y+ + Y + GD+ Y + M H+ID T + E + + G+R Sbjct: 80 LNRQTYLFKFLVVYIVEGDKSYLRQMKYFMYHWID-----CQFTLKPEGAVSRTIDTGIR 134 Query: 179 LFMLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVRKYHATHGNHVCMQLRGLIQTA-- 236 + I K+KL+ S E Y+R Y+ + + G++QT Sbjct: 135 CMSWLKVLIFLDYFGLITETKKIKLLTSLREQITYMRDYYREKDS---LSNWGILQTTAI 191 Query: 237 ----------LLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFEGSPNYHLVVMRDLY 286 L LPE++ AE E+ I+ + DG Q+E S YH+ V++ L Sbjct: 192 LACLYYYEDELNLPEIQSFAE------EELLLQIKLQILDDGSQYEQSIMYHVEVLKSLM 245 Query: 287 ELVALFHKLGIAAGEYQEILENMFVVMMHLLAPDGQLVKFGDTDQQVASELRNVMSLGAY 346 ELV L K + +E +E M ++ + PD + GD+D ++ R++++L Sbjct: 246 ELVILAPKYYLPL---EETIEKMVTYLIAMTGPDYCQLAIGDSD---VTDTRDILTLATL 299 Query: 347 LYQRSDFKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAACFPAGGYLTSRQGWKH 406 + + S K+ + + + G ++ + ++ E+A FP G++ R + Sbjct: 300 VLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIPRATIGESAYLFPDSGHVCLRDDRR- 358 Query: 407 TDMYMAMRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFEWDKERKYVVSTRAHNTV 466 Y+ + G H HSD S+ LY + + D+G ++ ++ R + +H+T Sbjct: 359 ---YIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDAGRYTYKEEQLRYDFKRSTSHSTC 415 Query: 467 VVDGQDQHVRSFHWNTPATAACRIWDFQTNEQYDYTFASHYGYTRYDDPVIHSRKVLFVK 526 +DGQ + W + C + ++Y + R D H R +L + Sbjct: 416 TLDGQPLEMIKDSWTYNSYPKCDYCQLTSKDRY-HLVEGQLHVQRASDIYYHKRWLLTLP 474 Query: 527 NSYWLIVDLFEAKGQHRYEQYFHLPPGGVAYDCFTGEVSTQLEDANLLLVHPSAGQEMDQ 586 + L++D G+H + L + + F + L LV P+ Sbjct: 475 QAITLVIDKVSCPGEHVLTNQYILDDQVIYENGFVND---------LKLVSPTT------ 519 Query: 587 LSVEPGLVFKQGNYY--DHPVVKRSLTAAGRTVIATLAVPFGTAKPKVKVERLPARRNGA 644 ++E L+ K+ N H +VK+ + + TL V +VK L + Sbjct: 520 FNLEDCLISKRYNQLTESHKLVKK-IKFVDEVMDYTLIVDRNC---QVKCVPLVQTNSHK 575 Query: 645 ELSAAEATALR 655 ELS + A +R Sbjct: 576 ELSNSIAFDIR 586 >ref|NP_688886.1| conserved hypothetical protein [Streptococcus agalactiae 2603V/R] gb|AAN00759.1| conserved hypothetical protein [Streptococcus agalactiae 2603V/R] Length = 634 Score = 115 bits (289), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 108/496 (21%), Positives = 203/496 (40%), Gaps = 38/496 (7%) Query: 59 LKAYAVNSYSQEEEAKQDIAEADRIAEGDIPLFKGKRVVFPKGTYEWNSWLYDSSQYQLH 118 +K Y N+Y QE +A ++ + D + + EW+ + D ++ Sbjct: 21 IKTYQSNAY-QEMKASVNLMMRNTFVFNDNWDMEPCSKAYCLDPLEWDKPVTDDPEWLYM 79 Query: 119 LTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDNPMPVDGTFRAEHSTWDPLSVGVR 178 L R Y+ + Y + GD+ Y + M H+ID T + E + + G+R Sbjct: 80 LNRQTYLFKFLVVYIVEGDKSYLRQMKYFMYHWID-----CQFTLKPEGAVSRTIDTGIR 134 Query: 179 LFMLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVRKYHATHGNHVCMQLRGLIQTA-- 236 + I K+KL+ S E Y+R Y+ + + G++QT Sbjct: 135 CMSWLKVLIFLDYFGLITETKKIKLLTSLREQITYMRDYYREKDS---LSNWGILQTTAI 191 Query: 237 ----------LLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFEGSPNYHLVVMRDLY 286 L LPE++ AE E+ I+ + DG Q+E S YH+ V++ L Sbjct: 192 LACLYYYEDELNLPEIQSFAE------EELLLQIKLQILDDGSQYEQSIMYHVEVLKSLM 245 Query: 287 ELVALFHKLGIAAGEYQEILENMFVVMMHLLAPDGQLVKFGDTDQQVASELRNVMSLGAY 346 ELV L K + +E +E M ++ + PD + GD+D ++ R++++L Sbjct: 246 ELVILAPKYYLPL---EETIEKMVTYLIAMTGPDYCQLAIGDSD---VTDTRDILTLATL 299 Query: 347 LYQRSDFKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAACFPAGGYLTSRQGWKH 406 + + S K+ + + + G ++ + ++ E+A FP G++ R + Sbjct: 300 VLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIPRATIGESAYLFPDSGHVCLRDDRR- 358 Query: 407 TDMYMAMRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFEWDKERKYVVSTRAHNTV 466 Y+ + G H HSD S+ LY + + D+G ++ ++ R + +H+T Sbjct: 359 ---YIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDAGRYTYKEEQLRYDFKRSTSHSTC 415 Query: 467 VVDGQDQHVRSFHWNTPATAACRIWDFQTNEQYDYTFASHYGYTRYDDPVIHSRKVLFVK 526 +DGQ + W + C + ++Y + R D H R +L + Sbjct: 416 TLDGQPLEMIKDSWTYNSYPKCDYCQLTSKDRY-HLVEGQLHVQRASDIYYHKRWLLTLP 474 Query: 527 NSYWLIVDLFEAKGQH 542 + L++D G+H Sbjct: 475 QAITLVIDKVSCPGEH 490 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,125,823,200 Number of extensions: 49122913 Number of successful extensions: 99421 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 99378 Number of HSP's successfully gapped: 28 Length of query: 718 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 585 Effective length of database: 396,129,411 Effective search space: 231735705435 Effective search space used: 231735705435 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)