BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0034.p (228 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060284.1| hypothetical protein [Clostridium thermocellu... 160 2e-38 >ref|ZP_00060284.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 228 Score = 160 bits (406), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 82/222 (36%), Positives = 127/222 (57%), Gaps = 5/222 (2%) Query: 9 WNAIHQQLTRIITKPEEHANAVALFLELHDCLYASNGNNPEQTNYEDELLKDLREQTMRS 68 WN ++L II ++ + L +LH +++ + EDEL + L ++T + Sbjct: 8 WNPKQKRLKNIILNEDKFNETIELIQDLHSMVHSHDVYGRNIPTLEDELWEGLDKKTFVT 67 Query: 69 YPVHTANSNNSIVWHIWHSARIEDITMNILTADRDQILHSGHYADKLHTPFIHSGNGMSG 128 P + +I W+IWH RIED+T+NIL AD Q ++ +L+ +GN MS Sbjct: 68 MP---TKEDVTIAWNIWHITRIEDLTVNILIADEIQEFKRQNWPARLNIEVSDTGNAMSD 124 Query: 129 QEIAEFSAAVDLEALLEYRQAVAAGTRSIIAGLQPGQFRNKPRSTQISRISAEGAVKETE 188 EI S +++E L EYR+AV TR +I L+P F+ K + T+++RI EG V + E Sbjct: 125 DEIISLSEKINMEELREYRKAVGKRTREVILKLKPDDFKKKVQKTRLNRILDEGGVLDVE 184 Query: 189 --QWLIDYWGGKTIAGLVLMPATRHNFVHLNKAMRVKLKLQK 228 +WL+D+WG K IAGL+LMP TRH VHLN ++++K K + Sbjct: 185 DSKWLLDFWGKKNIAGLILMPITRHQVVHLNDSLKLKKKCSR 226 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.131 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 326,739,328 Number of extensions: 12548468 Number of successful extensions: 30503 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 30506 Number of HSP's successfully gapped: 1 Length of query: 228 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 107 Effective length of database: 428,710,599 Effective search space: 45872034093 Effective search space used: 45872034093 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)