BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0021.p (349 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_692007.1| hypothetical protein OB1086 [Oceanobacillus ihe... 159 9e-38 ref|NP_241888.1| BH1022~unknown conserved protein in bacilli [Ba... 154 4e-36 sp|P32814|YGL4_BACST Hypothetical 35.5 kDa protein in GLDA 3'reg... 153 5e-36 ref|NP_388773.1| alternate gene name: ygaQ~similar to hypothetic... 151 2e-35 emb|CAB07515.1| hypothetical 35.8 kd protein [Bacillus subtilis] 151 2e-35 >ref|NP_692007.1| hypothetical protein OB1086 [Oceanobacillus iheyensis HTE831] dbj|BAC13042.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 285 Score = 159 bits (402), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 77/152 (50%), Positives = 101/152 (66%), Gaps = 6/152 (3%) Query: 196 WWKDGNPEFEVFDVDCTNYVSQCLFAGGAPINYTGKRETGWWYKGYNGAQEWWSFSWAVS 255 WW NP + F+VDCTNY+SQCLFAGGAP RE GWWY G + WSFSWAV+ Sbjct: 139 WWNSYNPAYRTFEVDCTNYISQCLFAGGAPTWGEPVRERGWWYSGNS-----WSFSWAVA 193 Query: 256 DSLQRYLSAGRSSGLRAETVERADQLMLGDIIQYDWDGNGHYQHSTIVTAFDAAGQPLVN 315 +++ YLS G GL+ + VE ++L+ GD+I YD++G+ + H+TIV DA G PLVN Sbjct: 194 HAMRWYLS-GSQKGLKGKEVESPEELLPGDVICYDFEGDNRWNHTTIVVTKDAYGMPLVN 252 Query: 316 ARTVSSRHRFWDYRDSYAWTDRTAYRFFHIND 347 A T +SRHR+W Y DS A+T Y+FF I + Sbjct: 253 AHTDNSRHRYWSYEDSTAYTPEIQYKFFRIGE 284 >ref|NP_241888.1| BH1022~unknown conserved protein in bacilli [Bacillus halodurans] pir||F83777 hypothetical protein BH1022 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04741.1| BH1022~unknown conserved protein in bacilli [Bacillus halodurans] Length = 298 Score = 154 bits (388), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 73/152 (48%), Positives = 101/152 (66%), Gaps = 6/152 (3%) Query: 196 WWKDGNPEFEVFDVDCTNYVSQCLFAGGAPINYTGKRETGWWYKGYNGAQEWWSFSWAVS 255 WW D NP ++ F+ +CTN++SQCL AGGAP R GWWY+ N WS+SW V+ Sbjct: 151 WWNDYNPNYKNFENNCTNFISQCLRAGGAPTTGAPNRSKGWWYQNNN-----WSYSWTVA 205 Query: 256 DSLQRYLSAGRSSGLRAETVERADQLMLGDIIQYDWDGNGHYQHSTIVTAFDAAGQPLVN 315 ++++ +LS R+ GLR E + L+ GD+I YD++G+G +QH+TIV A D G PLVN Sbjct: 206 NAMRWHLSGART-GLRGEERKSPRDLIPGDVICYDFNGDGRWQHTTIVVAKDVNGDPLVN 264 Query: 316 ARTVSSRHRFWDYRDSYAWTDRTAYRFFHIND 347 A+T +SR R+W Y DS A+T Y+FFHI D Sbjct: 265 AQTTNSRMRYWAYEDSTAYTPNIQYKFFHIVD 296 >sp|P32814|YGL4_BACST Hypothetical 35.5 kDa protein in GLDA 3'region (ORF4) pir||JS0681 hypothetical protein 4 (gldA 5' region) - Bacillus stearothermophilus gb|AAA22479.1| ORF4 Length = 301 Score = 153 bits (387), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 75/152 (49%), Positives = 97/152 (63%), Gaps = 6/152 (3%) Query: 196 WWKDGNPEFEVFDVDCTNYVSQCLFAGGAPINYTGKRETGWWYKGYNGAQEWWSFSWAVS 255 WW NP + FDV+CTNYVSQCL AG AP+ R +GWW N WS+SW+V+ Sbjct: 151 WWNSRNPNYPYFDVNCTNYVSQCLRAGDAPMRGYPNRSSGWWLANNN-----WSYSWSVA 205 Query: 256 DSLQRYLSAGRSSGLRAETVERADQLMLGDIIQYDWDGNGHYQHSTIVTAFDAAGQPLVN 315 +L YLS ++ GLRAE V + L GD+I YD+ G+G + HST V A D + PLVN Sbjct: 206 HALMLYLSTSKT-GLRAEEVSSPELLKPGDVICYDFQGDGRFDHSTFVVAKDKSDMPLVN 264 Query: 316 ARTVSSRHRFWDYRDSYAWTDRTAYRFFHIND 347 A+T +SR R+W Y DS A+T+ Y+FFHI D Sbjct: 265 AQTSNSRMRYWSYEDSTAYTENIQYKFFHIID 296 >ref|NP_388773.1| alternate gene name: ygaQ~similar to hypothetical proteins [Bacillus subtilis] pir||F69820 conserved hypothetical protein yhbB - Bacillus subtilis emb|CAB12720.1| yhbB [Bacillus subtilis subsp. subtilis str. 168] Length = 311 Score = 151 bits (382), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 73/152 (48%), Positives = 99/152 (65%), Gaps = 6/152 (3%) Query: 196 WWKDGNPEFEVFDVDCTNYVSQCLFAGGAPINYTGKRETGWWYKGYNGAQEWWSFSWAVS 255 +W NP ++ F +CTN++SQCL AGGAP+ R +GWW K Q WS+SW V+ Sbjct: 161 FWNKRNPAYKNFSDNCTNFISQCLHAGGAPMRGHPNRGSGWWMK-----QSSWSYSWTVA 215 Query: 256 DSLQRYLSAGRSSGLRAETVERADQLMLGDIIQYDWDGNGHYQHSTIVTAFDAAGQPLVN 315 S++ YLS ++ GLRA V+ A++LM GD+I YD++G+G + H+TIV A D PLVN Sbjct: 216 HSMKMYLSNSKA-GLRAVRVKSAEELMPGDVICYDFEGDGRFNHTTIVVAKDKGNMPLVN 274 Query: 316 ARTVSSRHRFWDYRDSYAWTDRTAYRFFHIND 347 A+T SR R+W Y DS A+T Y FFHI D Sbjct: 275 AQTYDSRMRYWSYEDSTAYTPSIRYAFFHIVD 306 >emb|CAB07515.1| hypothetical 35.8 kd protein [Bacillus subtilis] Length = 311 Score = 151 bits (381), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 73/152 (48%), Positives = 99/152 (65%), Gaps = 6/152 (3%) Query: 196 WWKDGNPEFEVFDVDCTNYVSQCLFAGGAPINYTGKRETGWWYKGYNGAQEWWSFSWAVS 255 +W NP ++ F +CTN++SQCL AGGAP+ R +GWW K Q WS+SW V+ Sbjct: 161 FWNKRNPAYKNFSDNCTNFISQCLHAGGAPMRGHPNRGSGWWMK-----QSSWSYSWTVA 215 Query: 256 DSLQRYLSAGRSSGLRAETVERADQLMLGDIIQYDWDGNGHYQHSTIVTAFDAAGQPLVN 315 S++ YLS ++ GLRA V+ A++LM GD+I YD++G+G + H+TIV A D PLVN Sbjct: 216 HSMKMYLSNSKA-GLRAVRVKSAEELMPGDVICYDFEGDGRFNHTTIVVAKDKGNMPLVN 274 Query: 316 ARTVSSRHRFWDYRDSYAWTDRTAYRFFHIND 347 A+T SR R+W Y DS A+T Y FFHI D Sbjct: 275 AQTYDSRMRYWSYEDSTAYTPSIRYAFFHIVD 306 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.133 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 525,468,770 Number of extensions: 20875022 Number of successful extensions: 41413 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 41390 Number of HSP's successfully gapped: 17 Length of query: 349 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 223 Effective length of database: 415,135,104 Effective search space: 92575128192 Effective search space used: 92575128192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)