BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0009.p (748 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_936111.1| glycogen debranching enzyme [Vibrio vulnificus ... 318 4e-85 ref|NP_763136.1| Glycogen debranching enzyme [Vibrio vulnificus ... 313 1e-83 ref|ZP_00019786.1| hypothetical protein [Chloroflexus aurantiacus] 156 2e-36 ref|NP_563252.1| probable glycogen debranching enzyme [Clostridi... 149 2e-34 ref|ZP_00149411.1| hypothetical protein Mbur042801 [Methanococco... 146 1e-33 >ref|NP_936111.1| glycogen debranching enzyme [Vibrio vulnificus YJ016] dbj|BAC96081.1| glycogen debranching enzyme [Vibrio vulnificus YJ016] Length = 926 Score = 318 bits (814), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 168/410 (40%), Positives = 242/410 (59%), Gaps = 8/410 (1%) Query: 235 WLNITMDQLVTRQQGDGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKF 294 W ++ V+ + G GI+AGLPWF + WGRD FIAL G L+ G F+ AK I+ +FA Sbjct: 395 WARLSSRTFVSHEFGVGIWAGLPWFKDCWGRDTFIALSGTSLINGLFDEAKAIISNFASM 454 Query: 295 QNTDAASKYFGRVPNILALK-NVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQN 353 Q ++ GR+PN + K N+ Y+TTDGTP I ++ +Y+ YSGD A ++YP VQ Sbjct: 455 QKSNLDDVNHGRIPNRVTSKTNIIYNTTDGTPWMIREIMEYLHYSGDMAFAQQIYPVVQR 514 Query: 354 SIEGSIKHWVDDKGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYFA 413 I+G KH++D+ G + H D +TWMDA+ +SPR +ANDIQALW+ LQ A Sbjct: 515 FIQGVEKHYLDEDGLMSHRDPDTWMDAKINGQIPWSPRGPKANDIQALWFESLQIAEQLA 574 Query: 414 EYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQLFALDM- 472 + GD A+ +A+K + +F F LAD L DE + SLRPNQL AL + Sbjct: 575 LWQGDEAFAQHCHQLATKAQESFVTKFWQPEERCLADCLRHGDEADLSLRPNQLMALTIP 634 Query: 473 IDDDSFKCEV----IKTAWEELVYPWGVASLDQKHPFFHPFHLT-SLYHKDEAYHNGTVW 527 + + E+ +K E+L++PWG+ SL Q H FHP+H S YHKD AYHNGT+W Sbjct: 635 MKRNLLAAEIGQHLVKNCVEQLLFPWGICSLAQSHQDFHPYHDNRSEYHKDAAYHNGTIW 694 Query: 528 LWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTGA 587 W G + + + GQ++ AY+L KN+ Q L G G +SEN+DA+ Q+ ++ +TG Sbjct: 695 GWNAGFTVSALCQFGQQDFAYQLSKNLAKQILTQGHRGTMSENLDAF-QQDENALVMTGT 753 Query: 588 YLQAWSNAEQLRVWYQYFLGFRPDMIHQALTLAPRIPEEIRDLQARVKIG 637 Y QAWS +E R Q +LGF P + + + L P++P ++AR+ G Sbjct: 754 YAQAWSVSEFARNAQQDYLGFMPRLAEKRMLLHPQLPARWHKVKARLPFG 803 >ref|NP_763136.1| Glycogen debranching enzyme [Vibrio vulnificus CMCP6] gb|AAO08126.1| Glycogen debranching enzyme [Vibrio vulnificus CMCP6] Length = 926 Score = 313 bits (801), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 166/410 (40%), Positives = 240/410 (58%), Gaps = 8/410 (1%) Query: 235 WLNITMDQLVTRQQGDGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKF 294 W + V+ + G GI+AGLPWF + WGRD FIAL G L+ G F+ AK I+ +FA Sbjct: 395 WARLASRTFVSHEFGVGIWAGLPWFKDCWGRDTFIALSGTSLINGLFDEAKAIISNFASM 454 Query: 295 QNTDAASKYFGRVPNILALK-NVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQN 353 Q ++ GR+PN + K N+ Y+TTDGTP I ++ +Y+ YSGD A ++YP VQ Sbjct: 455 QKSNLDDVNHGRIPNRVTSKTNIIYNTTDGTPWMIREIMEYLHYSGDMAFAQQIYPVVQR 514 Query: 354 SIEGSIKHWVDDKGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYFA 413 I+G KH++D+ G + H D +TWMDA+ +SPR +ANDIQALW+ LQ A Sbjct: 515 FIQGVEKHYLDEDGLMSHRDPDTWMDAKINGQIPWSPRGPKANDIQALWFESLQIAEQLA 574 Query: 414 EYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQLFALDM- 472 + GD A+ +A+K + +F F LAD L DE + S RPNQL AL + Sbjct: 575 LWQGDEAFAQHCHQLATKAQESFVTKFWQPEERCLADCLRHGDEADLSQRPNQLMALTIP 634 Query: 473 IDDDSFKCEV----IKTAWEELVYPWGVASLDQKHPFFHPFHLT-SLYHKDEAYHNGTVW 527 + + E+ +K ++L++PWG+ SL Q H FHP+H S YHKD AYHNGT+W Sbjct: 635 MKRNLLAAEIGQHLVKNCVDQLLFPWGICSLAQSHQDFHPYHDNRSEYHKDAAYHNGTIW 694 Query: 528 LWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTGA 587 W G + + + GQ++ AY+L KN+ Q L G G +SEN+DA+ Q+ ++ +TG Sbjct: 695 GWNAGFTVSALCQFGQQDFAYQLSKNLAKQILTQGHRGTMSENLDAF-QQDENALVMTGT 753 Query: 588 YLQAWSNAEQLRVWYQYFLGFRPDMIHQALTLAPRIPEEIRDLQARVKIG 637 Y QAWS +E R Q +LGF P + + + L P++P ++AR+ G Sbjct: 754 YAQAWSVSEFARNAQQDYLGFMPRLAEKRMLLHPQLPARWHKVKARLPFG 803 >ref|ZP_00019786.1| hypothetical protein [Chloroflexus aurantiacus] Length = 667 Score = 156 bits (395), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 122/388 (31%), Positives = 181/388 (46%), Gaps = 37/388 (9%) Query: 236 LNITMDQLV------TRQQGDGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILL 289 L + DQ + T +G I AG PWF + WGRD I+LPG + T + E A +L Sbjct: 289 LALAADQFIVDRPLDTGLRGRTIIAGYPWFTD-WGRDTMISLPGLTIPTRRPEVAAAVLR 347 Query: 290 SFAKFQNTDAASKYFGRVPNILALKNV--DYHTTDGTPRFIIQLQDYVKYSGDTAVITEL 347 +FA+F + G +PN + V +Y+T D T F ++ + GD +ITEL Sbjct: 348 TFARFVDQ-------GMLPNRFPDEGVAPEYNTADATLWFFEAIRAHHAAFGDDDLITEL 400 Query: 348 YPAVQNSIEGSIKHWVDDKGYLMHDD---------NETWMDARDANLKSYSPRDTRANDI 398 +P ++ I+ I+ D G D TWMD R +PR + +I Sbjct: 401 FPLLEEIIDWHIRGTRDGIGLDRGDSLLRAGTATTQLTWMDVRVRGW-VVTPRSGKPVEI 459 Query: 399 QALWYNQLQAGVYFAEYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDEP 458 ALWYN LQ+ FA ++G + +++ +A+ K F + H YL D ++ D Sbjct: 460 NALWYNALQSMAAFARHLGKAD--ERYTTMAAATKAGFNRFWYADGH-YLYDVIDGPDGV 516 Query: 459 EFSLRPNQLFALDMID---DDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLTSLY 515 + +LRPNQ+ A+ + D + V++ L+ G+ SL HP + P + SL Sbjct: 517 DPTLRPNQIIAVSLPYSPLDRQRQEAVVQACARSLLTGVGLRSLTPDHPDYAPRYTGSLL 576 Query: 516 HKDEAYHNGTVWLWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSENMDAYP 575 +D AYH GTVW WL G ++ A + L G VG +SE D P Sbjct: 577 ARDAAYHQGTVWAWLIGPFASAWYRITRDRDATRRLLLPLIDHLVDGCVGTISEIFDGDP 636 Query: 576 QEGQSWAKLTGAYLQAWSNAEQLRVWYQ 603 + G + QAWS AE LRVWY+ Sbjct: 637 PH-----QPRGCFAQAWSVAEVLRVWYE 659 >ref|NP_563252.1| probable glycogen debranching enzyme [Clostridium perfringens] dbj|BAB82042.1| probable glycogen debranching enzyme [Clostridium perfringens str. 13] Length = 672 Score = 149 bits (377), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 122/408 (29%), Positives = 199/408 (48%), Gaps = 61/408 (14%) Query: 235 WLNITMDQLVTRQQ---GDGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSF 291 +L I DQ + ++ G I AG PWF + WGRD IA+ G L TG+ E AK+I+ SF Sbjct: 277 YLPIAGDQFIVNRESIKGKTILAGYPWFLD-WGRDAMIAINGLTLSTGRLEDAKDIIRSF 335 Query: 292 AKFQNTDAASKYFGRVPNILALKNVD--YHTTDGTPRFIIQLQDYVKYSGDTAVI----T 345 + ++ G +PN+ K + Y+T D + FI + +Y+ Y+ + Sbjct: 336 SLYEKD-------GLIPNMFPGKGQEPLYNTVDASLWFINAVYNYLLYANSYEALEFVEK 388 Query: 346 ELYPAVQNSIEG-------SIKHWVDDKGYLMHD----DNETWMDARDANLKSYSPRDTR 394 E+Y ++N I+ SIK +D++ L++ D TWMD R N +PR + Sbjct: 389 EVYKTIKNIIKAYKEGTKFSIK--MDEEDSLINAGSGLDQVTWMDVR-VNGIVVTPRHGK 445 Query: 395 ANDIQALWYNQLQAGVYFAEYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNP 454 +I ALWYN L+ + +A K+K +F F ++ YL D +N Sbjct: 446 PVEINALWYNALKIAALLKNKFEKEEKNEY-EELAKKVKDSFTKTFWNEDRKYLYDVVNN 504 Query: 455 EDEPEFSLRPNQLFALD----MIDDDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFH 510 ++ + SLRPNQ++A+ M+D + K +++ +EEL P+G+ SL + + +H + Sbjct: 505 HEKDD-SLRPNQIWAVSLPFTMLDREKEK-HIVQKVFEELYTPYGLRSLSRDNKDYHGIY 562 Query: 511 LTSLYHKDEAYHNGTVWLWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSEN 570 + L+ +D AYH GT W + G TAY + + +A++L V L + Sbjct: 563 IGKLFDRDMAYHQGTTWAF----------PLGGFFTAYCKVNDYSKEAVDL--VDSLMRD 610 Query: 571 MDAYPQ-----------EGQSWAKLTGAYLQAWSNAEQLRVWYQYFLG 607 M+ + + +G + K G Y QAWS E LRV+Y+ LG Sbjct: 611 MEDHIKDQCLGSIAEIFDGDNPYKARGCYAQAWSVGEMLRVYYEDILG 658 >ref|ZP_00149411.1| hypothetical protein Mbur042801 [Methanococcoides burtonii DSM 6242] Length = 602 Score = 146 bits (369), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 117/370 (31%), Positives = 179/370 (48%), Gaps = 39/370 (10%) Query: 251 GIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKFQNTDAASKYFGRVPNI 310 I AG WF + WGRD I+LPG LVTG+F AK IL +F ++ S+ G +PN Sbjct: 241 SILAGYHWFAD-WGRDAMISLPGLTLVTGRFNDAKAILTTF-----SEQCSQ--GLIPNR 292 Query: 311 LA---LKNVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAV----QNSIEGSIKHW- 362 + + DY+T D + FI L+ Y Y+ D + +++ V N IEG++ Sbjct: 293 FSDDGISPTDYNTVDASLWFIHSLERYYSYTRDIEFVKKMWDTVGSILHNYIEGTLYGIR 352 Query: 363 VDDKGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYFAEYMGDSGSA 422 +DD G + TWMD + + + +PR +A +I ALWYN L F E +G Sbjct: 353 MDDDGLIEQKGQLTWMDVKIGD-REITPRAGKACEINALWYNSLCIANLFGELIG--ADT 409 Query: 423 KKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDE----PEFSLRPNQLFALD----MID 474 K++ I+ K NF F + T L D ++ +++ + S+RPNQ+FA+ M++ Sbjct: 410 KQYLEISRLAKSNFVDTFWNSTDKCLYDCVSVDEDMAVIKDASIRPNQIFAVSLPYTMLN 469 Query: 475 DDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLTSLYHKDEAYHNGTVWLWLNGIA 534 D K +++ EEL+ P+G+ +L + + +D AYHNGTVW WL G Sbjct: 470 PDKEKM-IVEKVVEELLTPYGLRTLSPADSQYIGRYRGDRDSRDRAYHNGTVWPWLLGPF 528 Query: 535 MQRMIEAGQE-----ETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTGAYL 589 + + E + +L N G +G +SE D G + + G Sbjct: 529 VTAYTKVHNRSPSSLEYSRQLLLNFGPHFDEAG-IGTISEIFD-----GDTPHRPDGCIS 582 Query: 590 QAWSNAEQLR 599 QAWS AE LR Sbjct: 583 QAWSVAEILR 592 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,092,548,795 Number of extensions: 45496650 Number of successful extensions: 101761 Number of sequences better than 1.0e-05: 51 Number of HSP's gapped: 101620 Number of HSP's successfully gapped: 52 Length of query: 748 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 615 Effective length of database: 396,129,411 Effective search space: 243619587765 Effective search space used: 243619587765 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)