BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0004.p (205 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_487015.1| signal peptidase I [Nostoc sp. PCC 7120] >gi|25... 126 3e-28 ref|ZP_00162253.1| hypothetical protein Avar004901 [Anabaena var... 123 2e-27 ref|ZP_00061349.1| COG0681: Signal peptidase I [Clostridium ther... 120 2e-26 ref|NP_656962.1| Peptidase_S26, Signal peptidase I [Bacillus ant... 117 1e-25 emb|CAD70602.1| type I signal peptidase [Bacillus megaterium] 117 1e-25 >ref|NP_487015.1| signal peptidase I [Nostoc sp. PCC 7120] pir||AH2177 hypothetical protein alr2975 [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB74674.1| ORF_ID:alr2975~signal peptidase I [Nostoc sp. PCC 7120] Length = 190 Score = 126 bits (317), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 66/182 (36%), Positives = 101/182 (55%), Gaps = 8/182 (4%) Query: 24 RTGSLSGTGWVRDLRDWLVTAAIVFALMSLLNIFVFNVSTVIGQSMQPTLFEGEKLIISK 83 + S W R ++ L AI L L+ F+ + +SM PTL+EG++L++ K Sbjct: 9 KQASTPSKAW-RGWQENLTLIAIALCLALLIRTFIAEPRYIPSESMVPTLYEGDRLVVEK 67 Query: 84 ISLTFASPRRSDVVVLHDPST----GPDRKEYLVKRVIGIPGDRIEVRNHKLYVNGKLIG 139 +S F P D+VV P+ G + + +KRVIG PG+ I V N K+Y+NGK + Sbjct: 68 VSYNFQQPTTGDIVVFQPPAELQRRGYPKDQAFIKRVIGTPGEIISVNNGKVYLNGKALP 127 Query: 140 ESYVDTEIQDPDFAELTVKQGTYFVMGDNRHAGASKDSRYFGAVPQNRIVGKAAYIWWPL 199 E Y+ P F + V + +FVMGDNR+ S DSRY+G +P+ I+G+A + +WPL Sbjct: 128 EDYIAEPPNQP-FPPVKVPENQFFVMGDNRN--NSNDSRYWGFLPKENIIGRAVFRFWPL 184 Query: 200 SK 201 + Sbjct: 185 DR 186 >ref|ZP_00162253.1| hypothetical protein Avar004901 [Anabaena variabilis ATCC 29413] Length = 190 Score = 123 bits (309), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 67/181 (37%), Positives = 102/181 (56%), Gaps = 11/181 (6%) Query: 25 TGSLSGTGWVRDLRDWLVTAAIVFALMSLLNIFVFNVSTVIGQSMQPTLFEGEKLIISKI 84 T S + GW +L L+ A+ AL L+ F+ + +SM PTL+EG++L++ K+ Sbjct: 13 TPSKAWRGWQENLT--LIAIALCLAL--LIRTFIAEPRYIPSESMVPTLYEGDRLVVEKV 68 Query: 85 SLTFASPRRSDVVVLHDPST----GPDRKEYLVKRVIGIPGDRIEVRNHKLYVNGKLIGE 140 S F P D+VV P+ G + + +KRVI PG+ I V N K+Y+NGK + E Sbjct: 69 SYNFQQPTTGDIVVFQPPAELQRRGYPKDQAFIKRVIATPGEIISVNNGKVYLNGKALPE 128 Query: 141 SYVDTEIQDPDFAELTVKQGTYFVMGDNRHAGASKDSRYFGAVPQNRIVGKAAYIWWPLS 200 Y+ P F + V +FVMGDNR+ S DSRY+G +P+ I+G+A + +WPL Sbjct: 129 DYIAEPPNQP-FPPVKVPDNQFFVMGDNRN--NSNDSRYWGFLPKENIIGRAVFRFWPLD 185 Query: 201 K 201 + Sbjct: 186 R 186 >ref|ZP_00061349.1| COG0681: Signal peptidase I [Clostridium thermocellum ATCC 27405] Length = 164 Score = 120 bits (301), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 63/151 (41%), Positives = 99/151 (65%), Gaps = 7/151 (4%) Query: 57 FVFNVSTVIGQSMQPTLFEGEKLIISKISLTFASPRRSDVVVLHD-PSTGPDRKEYLVKR 115 FV ++ V G SM+ TL G++LII KIS F +R D+V ++D P DRK ++KR Sbjct: 10 FVAQITIVNGSSMETTLHNGDRLIIEKISPRFGWLKRGDIVTINDYPGLDSDRKP-IIKR 68 Query: 116 VIGIPGDRIEVRNHKLYVNGKLIGESYVDTEIQ-----DPDFAELTVKQGTYFVMGDNRH 170 +IG+ GD++E+R+ K+YVNG+ + E Y++ +++ + +++EL V +G +V+GDNR Sbjct: 69 IIGLEGDKVEIRDGKVYVNGEALEEDYINVDVEGTLEVNENYSELYVPEGHIYVLGDNRL 128 Query: 171 AGASKDSRYFGAVPQNRIVGKAAYIWWPLSK 201 G SKDSR FG V + GKA + ++PL K Sbjct: 129 PGQSKDSRTFGPVDIKNVGGKAIFRFFPLDK 159 >ref|NP_656962.1| Peptidase_S26, Signal peptidase I [Bacillus anthracis A2012] ref|NP_845426.1| Signal peptidase I U [Bacillus anthracis str. Ames] ref|NP_979425.1| Signal peptidase I U [Bacillus cereus ATCC 10987] gb|AAP26912.1| Signal peptidase I U [Bacillus anthracis str. Ames] gb|AAS42033.1| Signal peptidase I U [Bacillus cereus ATCC 10987] Length = 183 Score = 117 bits (294), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 68/184 (36%), Positives = 100/184 (54%), Gaps = 20/184 (10%) Query: 35 RDLRDWLVTAAIVFALMSLLNIFVFNVSTVIGQSMQPTLFEGEKLIISKISLTFASPRRS 94 ++L W T L++++ +F S V G+SM PTL E+++++KI + + R Sbjct: 7 KELFSWAKTIGFTLVLIAIIRGVLFTPSLVQGESMMPTLENNERVLVNKIGYSISGLERF 66 Query: 95 DVVVLHDPSTGPDRKEYLVKRVIGIPGDRIEVRNHKLYVNGKLIGESYVDT-------EI 147 D++V H G D LVKRVIG+PGD +E +N LYVNGK + E Y+ + Sbjct: 67 DIIVFHG-KEGYD----LVKRVIGLPGDTVEYKNDVLYVNGKAMEEPYLKEFKEKAAGRV 121 Query: 148 QDPDF------AELTVKQGTYFVMGDNRHAGASKDSRYFGAVPQNRIVGKAAYIWWPLSK 201 PDF + V +G FV+GDNR SKD R FG + ++ IVGK ++WPL + Sbjct: 122 LTPDFTLEQITGKTKVPEGQVFVLGDNRE--VSKDGRMFGFISEDEIVGKGQAVFWPLKQ 179 Query: 202 ANAL 205 AL Sbjct: 180 VRAL 183 >emb|CAD70602.1| type I signal peptidase [Bacillus megaterium] Length = 183 Score = 117 bits (294), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 69/180 (38%), Positives = 100/180 (55%), Gaps = 20/180 (11%) Query: 36 DLRDWLVTAAIVFALMSLLNIFVFNVSTVIGQSMQPTLFEGEKLIISKISLTFASPRRSD 95 +L +W+ AI L ++ F+F V G SM PTL + ++I++K S P R D Sbjct: 7 ELWEWIKAIAIAVLLAVVIRYFLFAPIVVDGLSMMPTLHDQNRMIVNKFSYKIGDPDRFD 66 Query: 96 VVVLHDPSTGPDRKEYLVKRVIGIPGDRIEVRNHKLYVNGKLIGESYVDT---EIQDPDF 152 ++V H + K+Y +KR+IG+PGD IE RN LYVNGK E Y+D E+ D + Sbjct: 67 IIVFHATA----EKDY-IKRIIGLPGDHIEYRNDTLYVNGKAYKEPYLDQYKKEVIDGNL 121 Query: 153 AE----------LTVKQGTYFVMGDNRHAGASKDSRYFGAVPQNRIVGKAAYIWWPLSKA 202 E TV +G FVMGDNR SKDSR G V ++++GK + ++WP+ +A Sbjct: 122 TEDFKLEDVTGKKTVPEGYLFVMGDNRR--YSKDSRQIGFVSMDKVLGKTSAVYWPIKEA 179 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 305,875,070 Number of extensions: 12312272 Number of successful extensions: 29600 Number of sequences better than 1.0e-05: 427 Number of HSP's gapped: 29214 Number of HSP's successfully gapped: 428 Length of query: 205 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 85 Effective length of database: 431,425,698 Effective search space: 36671184330 Effective search space used: 36671184330 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)