BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0019.p (729 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00099967.1| COG0840: Methyl-accepting chemotaxis protein ... 190 1e-46 ref|NP_350063.1| Methyl-accepting chemotaxis protein [Clostridiu... 168 4e-40 ref|NP_782459.1| methyl-accepting chemotaxis protein [Clostridiu... 157 1e-36 ref|NP_347181.1| Methyl-accepting chemotaxis protein [Clostridiu... 146 1e-33 ref|NP_348227.1| Methyl-accepting chemotaxis-like protein (chemo... 130 1e-28 >ref|ZP_00099967.1| COG0840: Methyl-accepting chemotaxis protein [Desulfitobacterium hafniense] Length = 612 Score = 190 bits (483), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 146/507 (28%), Positives = 231/507 (45%), Gaps = 21/507 (4%) Query: 176 ITQDEFEPPADYNLKERGWYSLTAKNKRTTV-TDPYIDAGSGKMVVSVSAPILDESGKLI 234 I ++F PP E+GWY V TDPY+D S M+ S D GKL+ Sbjct: 66 IPVEKFYPP------EKGWYKTGLNGNGAIVRTDPYLDPMSNTMITSNVRSFTDADGKLV 119 Query: 235 GVAGADISTEQITQALSAFNYNGSGYAVLIDKTGTFI---HHPDSDNILLKKMSDLGGNW 291 G G D+ I+ LS+ +GY++++ +G + ++P+++ L+++ ++ G Sbjct: 120 GTLGIDVQQSVISDMLSSMKTGETGYSMIVHTSGIILADGNNPENNFKQLEEI-NIAGLD 178 Query: 292 KMLGDQMVQWGSNVIRTEIDGVASYVSYAPVVENQWSVALIVPQKSAEQRLQGFQQXXXX 351 K+ D + + +DG V+ V W +A ++ + Q Sbjct: 179 KLGSDDLTPFD-----VMVDGAKYIVNPHKVDGTDWILASLMSESELTQGANSVALMMLI 233 Query: 352 XXXXXXXXXXXXXXXXXXXXXRPIPQLTAAFRQAMTGDLSARAK---VNAKGEIGVLAGG 408 PI + GD S + K E+G + Sbjct: 234 VSVVMLLLTTVLITITANKITTPIKKSAQYLELVANGDFSQEIDSRFLARKDEVGAITNA 293 Query: 409 FNELIASQQNMXXXXXXXXXXXXXAVGNAETNVSALDGSISEISAITEELSAGMEQTAAS 468 N + S + + V NV L S+ EIS T +++A E+T+A+ Sbjct: 294 INHMKNSIKQLVNSIKSESVAIEEKVHAVMDNVVDLSKSLEEISVTTGDVAASTEETSAA 353 Query: 469 MEELNAGTNEIEHAVSGIXXXXXXXXXXXXQIXXXXRAERLKEGAGRSRRQADEMYGQSE 528 EE++A T EIE AV I +I RAE K+ ++++A +++ +++ Sbjct: 354 SEEMSATTQEIEKAVQLIAGKSQQGAVSSQEITR--RAEDTKKNVHTAQKKAADIFVETQ 411 Query: 529 EKLRKAIEQSRSTAQIHALTGAILDIAAQTSLLSLNASIXXXXXXXXXXXXXXXXXXIRK 588 KL KAI +S+ +QI LT AI+ I QT+LL+LNA+I IRK Sbjct: 412 AKLEKAIVESKVVSQIDLLTDAIMQITEQTNLLALNAAIEAARAGEAGRGFSVVSEEIRK 471 Query: 589 LAENSRETAAEIQEVTGAVVAAVENLVEGAENMLRFVDRQVLKDYDSMQETGRQYSEDAG 648 LAE S+ +IQEVT V +AV+NL + + +L F+ V DY M + +YSEDA Sbjct: 472 LAEQSKGAVMQIQEVTTKVTSAVDNLSQSSNGLLSFMSVDVNNDYQVMLDVAERYSEDAN 531 Query: 649 YIHELVTDFSATSGQLLASIQSMHSAI 675 ++ ELV DFSAT+ QLL SIQ++ AI Sbjct: 532 FVDELVADFSATTEQLLVSIQNISDAI 558 >ref|NP_350063.1| Methyl-accepting chemotaxis protein [Clostridium acetobutylicum] pir||H97326 methyl-accepting chemotaxis protein [imported] - Clostridium acetobutylicum gb|AAK81403.1| Methyl-accepting chemotaxis protein [Clostridium acetobutylicum] Length = 582 Score = 168 bits (426), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 110/292 (37%), Positives = 159/292 (54%), Gaps = 4/292 (1%) Query: 438 ETN--VSALDGSISEISAITEELSAGMEQTAASMEELNAGTNEIEHAVSGIXXXXXXXXX 495 ETN VS L G + +IS+ TEELSAGME+TAAS +E+N+ T E+E A++ I Sbjct: 291 ETNKSVSYLKGELEDISSTTEELSAGMEETAASTQEMNSTTTEMEKALNNIASKALEGSK 350 Query: 496 XXXQIXXXXRAERLKEGAGRSRRQADEMYGQSEEKLRKAIEQSRSTAQIHALTGAILDIA 555 I RA LKE S A+E+ KL K++ QS++ QI+ LT +IL I Sbjct: 351 SVGDISK--RAHELKENFSVSYNNAEEVMDSVRVKLEKSLLQSKAVEQINELTDSILQIT 408 Query: 556 AQTSLLSLNASIXXXXXXXXXXXXXXXXXXIRKLAENSRETAAEIQEVTGAVVAAVENLV 615 +QT+LL+LNASI IRKLAENS+ T +IQE+T V+ +VE L Sbjct: 409 SQTNLLALNASIEAARAGEAGKGFAVVAEEIRKLAENSKVTVNKIQEITKVVINSVEGLS 468 Query: 616 EGAENMLRFVDRQVLKDYDSMQETGRQYSEDAGYIHELVTDFSATSGQLLASIQSMHSAI 675 + + +L+FV+ +V DY +M E QY DA ++E+V+DFSATS +LL+SIQ+M + Sbjct: 469 DNSNELLQFVEEEVNNDYRTMLEATDQYKLDADLVNEIVSDFSATSEELLSSIQNMVMTM 528 Query: 676 XXXXXXXXXXXXXXXXXXXXXXQIISKSGGIVAEMEEIKHSSEQLLHTVSKF 727 I++++ +V ++ S+ L VSKF Sbjct: 529 NSIAEATNEGAIGTGNIAEKVASIVNRANELVKIIDSTNEGSDNLYKMVSKF 580 >ref|NP_782459.1| methyl-accepting chemotaxis protein [Clostridium tetani E88] gb|AAO36396.1| methyl-accepting chemotaxis protein [Clostridium tetani E88] Length = 569 Score = 157 bits (396), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 113/341 (33%), Positives = 179/341 (52%), Gaps = 2/341 (0%) Query: 388 GDLSARAKVNAKGEIGVLAGGFNELIASQQNMXXXXXXXXXXXXXAVGNAETNVSALDGS 447 GDL+ KVN+K EI LA N+ I + +++ V +TNVS L+ Sbjct: 230 GDLTQEIKVNSKDEINDLAKSLNKFIQNIKDIIKKVNESADNMENIVDIIKTNVSDLNND 289 Query: 448 ISEISAITEELSAGMEQTAASMEELNAGTNEIEHAVSGIXXXXXXXXXXXXQIXXXXRAE 507 I E+SA TEELSA +EQTAAS EE++A + IE ++ I +I RAE Sbjct: 290 IEEVSATTEELSANIEQTAASAEEMSATSQNIEKSIQSIAQKSQEGVFQAGEINK--RAE 347 Query: 508 RLKEGAGRSRRQADEMYGQSEEKLRKAIEQSRSTAQIHALTGAILDIAAQTSLLSLNASI 567 K+ S+ +A+E+ ++ +L KAIE S+ QI+ L+ +I++I +QT+LL+LNA+I Sbjct: 348 DTKKNIQVSQEKAEEILTNTKTELEKAIESSKVVEQINILSESIMNITSQTNLLALNAAI 407 Query: 568 XXXXXXXXXXXXXXXXXXIRKLAENSRETAAEIQEVTGAVVAAVENLVEGAENMLRFVDR 627 IR+LAE S++ EIQ +T V+ +V++L + + N+L FV Sbjct: 408 EAARAGEAGKGFSVVAEEIRELAEQSKDAVTEIQSITNKVIESVKSLSDSSSNLLTFVST 467 Query: 628 QVLKDYDSMQETGRQYSEDAGYIHELVTDFSATSGQLLASIQSMHSAIXXXXXXXXXXXX 687 + DY +M +Y+ DA +I +VT+F++TS +LL+S+Q + S I Sbjct: 468 DIKNDYKTMLNVVDKYNGDANFIDSIVTEFTSTSEELLSSLQDVLSTIDGVAQASSEGAG 527 Query: 688 XXXXXXXXXXQIISKSGGIVAEMEEIKHSSEQLLHTVSKFK 728 +I KS ++ E + K + +L +SKFK Sbjct: 528 GTTDIANKIIEISDKSNDVLQEALKSKERANKLKKEISKFK 568 >ref|NP_347181.1| Methyl-accepting chemotaxis protein [Clostridium acetobutylicum] pir||F96966 methyl-accepting chemotaxis protein [imported] - Clostridium acetobutylicum gb|AAK78521.1| Methyl-accepting chemotaxis protein [Clostridium acetobutylicum] Length = 575 Score = 146 bits (369), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 102/323 (31%), Positives = 167/323 (51%), Gaps = 36/323 (11%) Query: 373 RPIPQLTAAFRQAMT------GDLSARAKVNAKGEIGVLAGGFNELIASQQNMXXXXXXX 426 RPI + + A ++ ++ GDLS R VN K EIG L G + I +++ Sbjct: 216 RPIVKASRAIKEIISDIENNDGDLSKRIYVNTKDEIGELTLGVDAFIERLEDI------- 268 Query: 427 XXXXXXAVGNAETNVSALDGS--------------ISEISAITEELSAGMEQTAASMEEL 472 +G T+ + L+GS + E SA +E++AGME+TAAS+EE+ Sbjct: 269 -------IGKIRTSSATLEGSSKLLLEQISSSNIKVEETSATVQEIAAGMEETAASVEEI 321 Query: 473 NAGTNEIEHAVSGIXXXXXXXXXXXXQIXXXXRAERLKEGAGRSRRQADEMYGQSEEKLR 532 NA T++IE+AV I ++ +A +K A S++ A E+ +E L Sbjct: 322 NASTDDIENAVEKIALKAQSGSVATQKVNS--KANEIKVKAVSSQKAASEIIQVVQENLE 379 Query: 533 KAIEQSRSTAQIHALTGAILDIAAQTSLLSLNASIXXXXXXXXXXXXXXXXXXIRKLAEN 592 KA+ +S+ +I+ LT +IL+I QT++L+LNA+I I+ LAE+ Sbjct: 380 KALVKSKDVEKINLLTSSILEITEQTNVLALNAAIEASRAGEAGKGFAVVADEIKALAED 439 Query: 593 SRETAAEIQEVTGAVVAAVENLVEGAENMLRFVDRQVLKDYDSMQETGRQYSEDAGYIHE 652 S A+EIQ + V+ +V+NL ++++L F+ QV+KDYD +Y+ DA I + Sbjct: 440 SGRIASEIQSINSIVIESVKNLAGDSKSVLDFISNQVIKDYDMFVGVSEEYTNDATAISQ 499 Query: 653 LVTDFSATSGQLLASIQSMHSAI 675 L+ +F+ T+ QL S+ + AI Sbjct: 500 LMNNFNDTADQLKTSVNGIVIAI 522 >ref|NP_348227.1| Methyl-accepting chemotaxis-like protein (chemotaxis sensory transducer) [Clostridium acetobutylicum] pir||D97097 methyl-accepting chemotaxis-like protein (chemotaxis sensory transducer) [imported] - Clostridium acetobutylicum gb|AAK79567.1| Methyl-accepting chemotaxis-like protein (chemotaxis sensory transducer) [Clostridium acetobutylicum] Length = 673 Score = 130 bits (326), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 89/279 (31%), Positives = 137/279 (49%), Gaps = 2/279 (0%) Query: 450 EISAITEELSAGMEQTAASMEELNAGTNEIEHAVSGIXXXXXXXXXXXXQIXXXXRAERL 509 E S TE LSAGME+ +A++EE++A TNE+ ++S I + + Sbjct: 396 ETSGETENLSAGMEENSATVEEVSASTNEVNSSISNIAEKAENASELTYN--TNKMSSMI 453 Query: 510 KEGAGRSRRQADEMYGQSEEKLRKAIEQSRSTAQIHALTGAILDIAAQTSLLSLNASIXX 569 K+ S+R A+E+Y + L AI++S +I L AIL I QT+LL+LNA+I Sbjct: 454 KQDVVNSKRSANEIYANVKVGLESAIKKSEVVKEIDNLASAILQITEQTNLLALNAAIEA 513 Query: 570 XXXXXXXXXXXXXXXXIRKLAENSRETAAEIQEVTGAVVAAVENLVEGAENMLRFVDRQV 629 +R LAE S + A++IQ V V +V+ L + + +L+FVD V Sbjct: 514 ARAGEAGKGFAVVAEEVRTLAEQSSDMASKIQSVVETVNLSVKELNDQSVKLLKFVDENV 573 Query: 630 LKDYDSMQETGRQYSEDAGYIHELVTDFSATSGQLLASIQSMHSAIXXXXXXXXXXXXXX 689 KDYD + YS DA ++ L+++FS TS QL +SI + AI Sbjct: 574 YKDYDKFINSAETYSADAEKVNGLMSEFSTTSKQLNSSIDGISKAISEIAIVVNNGAIGV 633 Query: 690 XXXXXXXXQIISKSGGIVAEMEEIKHSSEQLLHTVSKFK 728 I+ K I +E K+S+ +L + +S+FK Sbjct: 634 GNIAEKSLNIVEKVKFIEESVENNKNSANELSNIISRFK 672 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.311 0.128 0.342 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 777,532,816 Number of extensions: 26298987 Number of successful extensions: 66231 Number of sequences better than 1.0e-05: 483 Number of HSP's gapped: 66241 Number of HSP's successfully gapped: 505 Length of query: 729 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 596 Effective length of database: 396,129,411 Effective search space: 236093128956 Effective search space used: 236093128956 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 130 (54.7 bits)