BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0013.p (421 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060128.1| COG0151: Phosphoribosylamine-glycine ligase [... 441 e-122 ref|NP_951668.1| phosphoribosylamine--glycine ligase [Geobacter ... 429 e-119 gb|AAN87361.1| Phosphoribosylamine--glycine ligase [Heliobacillu... 427 e-118 ref|NP_241500.1| phosphoribosylglycinamide synthetase [Bacillus ... 427 e-118 ref|NP_830173.1| Phosphoribosylamine--glycine ligase [Bacillus c... 422 e-117 >ref|ZP_00060128.1| COG0151: Phosphoribosylamine-glycine ligase [Clostridium thermocellum ATCC 27405] Length = 419 Score = 441 bits (1135), Expect = e-122, Method: Compositional matrix adjust. Identities = 224/421 (53%), Positives = 284/421 (67%), Gaps = 5/421 (1%) Query: 1 MDILVVGGGGREHAIIWSLSRSPKAGKIYCAPGNAGIAQLAECVPIGVFEFDKLTAFAKE 60 M +LVVG GGREHA++W + +SP+ K+YCAPGN GI+ +AECVPI + + + F+KE Sbjct: 1 MKVLVVGSGGREHALVWKIVQSPRVEKVYCAPGNGGISAIAECVPIKAMDIEGIVNFSKE 60 Query: 61 KSIDYVVIGPDDPLAAGIVDAFEAHHIPVFGPRKNAAEIEGSKTFMKDLLHKYSIPTAAY 120 K ID VV+ PDDPLAAG+VDA E I FGP K AA IE SK F K+L+ KY+IPTA Y Sbjct: 61 KKIDMVVVAPDDPLAAGMVDALEEAGIRAFGPNKAAAVIESSKAFAKNLMKKYNIPTARY 120 Query: 121 EKFSDYEAALSYLRGQGLPVVIKADGLAAGKGVTVAYSREEAELALRDIMVTKVFGEAGA 180 E F + A++YL+ Q PVV+KADGLA GKGV +A + +EA+ A++ IM KVFGEAG Sbjct: 121 EIFENSADAINYLQDQKYPVVVKADGLALGKGVIIAQNFDEAKQAVQSIMEDKVFGEAGN 180 Query: 181 QVVIEEFLAGQEMSILAFVDGETVRPMAAAQDHKPVFDGDKGPNTGGMGTYSPLPHIDEA 240 +VVIEEFL GQE+S+LAF DG+T++ M ++QDHK D D+G NTGGMGT+SP E Sbjct: 181 KVVIEEFLVGQEVSMLAFTDGKTIKTMVSSQDHKRALDNDQGLNTGGMGTFSPSRIYTEE 240 Query: 241 IIQEAVETIIKPTARAMVAEGRPFSGVLFAGLMISPDGKPKTIEFNARFGDPETQVVLPR 300 I + +E I KPT AM EGR F GVL+ GL+I+ DG PK +E+NARFGDPETQVVLPR Sbjct: 241 IDRYCMERIYKPTIEAMEKEGRKFKGVLYFGLIITKDG-PKVLEYNARFGDPETQVVLPR 299 Query: 301 LKSDLLEIFLAVTEGRLXXXXXXXXXXXXVCVVLASEGYPGPYPKGVPIAGLENSPND-- 358 L +D+++IF AV + RL VCV++AS GYP Y G I+G+E++ D Sbjct: 300 LNTDIIDIFEAVIDERLDEVEISWNDSACVCVIMASGGYPKEYKTGYEISGIEDAERDAN 359 Query: 359 -LVFHAGTERTEEGTWVTNGGRVLGVVGMGXXXXXXXXXXXXXXXKISFPGMHSRSDIAM 417 +VFHAGT+R E G + T GGRVLGV M KI F MH R DI Sbjct: 360 IVVFHAGTKR-ENGKYYTAGGRVLGVTAMENTLDEAIKKAYEGVGKIKFQDMHYRKDIGK 418 Query: 418 K 418 K Sbjct: 419 K 419 >ref|NP_951668.1| phosphoribosylamine--glycine ligase [Geobacter sulfurreducens PCA] gb|AAR33941.1| phosphoribosylamine--glycine ligase [Geobacter sulfurreducens PCA] Length = 423 Score = 429 bits (1103), Expect = e-119, Method: Compositional matrix adjust. Identities = 222/423 (52%), Positives = 290/423 (68%), Gaps = 5/423 (1%) Query: 1 MDILVVGGGGREHAIIWSLSRSPKAGKIYCAPGNAGIAQLAECVPIGVFEFDKLTAFAKE 60 M +LV+GGGGREHA++W +++SP K+YCAPGN GI +AE V + V + D L AFA E Sbjct: 1 MKVLVIGGGGREHALVWKIAQSPLVSKVYCAPGNPGIGLIAENVSLAVDDLDGLAAFATE 60 Query: 61 KSIDYVVIGPDDPLAAGIVDAFEAHHIPVFGPRKNAAEIEGSKTFMKDLLHKYSIPTAAY 120 +SID V+GP+ PL+ GIVD FE + +FG R+NAA IE SK F KDL+ KY +PTAAY Sbjct: 61 QSIDLTVVGPELPLSLGIVDRFEEKGLLIFGARRNAAIIEASKAFSKDLMKKYQVPTAAY 120 Query: 121 EKFSDYEAALSYLRGQGLPVVIKADGLAAGKGVTVAYSREEAELALRDIMVTKVFGEAGA 180 + F++ E A++++ G+P+V+KADGLAAGKGV +A++REEA A+ D++ FG+AG+ Sbjct: 121 DVFTEVEPAVAFIDRVGVPIVVKADGLAAGKGVIIAHTREEAVGAVMDMLSGNAFGDAGS 180 Query: 181 QVVIEEFLAGQEMSILAFVDGETVRPMAAAQDHKPVFDGDKGPNTGGMGTYSPLPHIDEA 240 +VVIEEFL G+E S LAF DG+ + P+A+AQDHK VFDGD GPNTGGMG YSP P + A Sbjct: 181 RVVIEEFLTGEEASFLAFTDGKNIIPLASAQDHKAVFDGDTGPNTGGMGAYSPAPVVTPA 240 Query: 241 IIQEAVETIIKPTARAMVAEGRPFSGVLFAGLMISPDGKPKTIEFNARFGDPETQVVLPR 300 I + + +++ T M AEGRP+ GVL+AGLMI+ D + KT+EFNARFGDPE Q +L R Sbjct: 241 IHDKVMAEVMRRTVDGMAAEGRPYRGVLYAGLMINGD-QVKTLEFNARFGDPECQPLLMR 299 Query: 301 LKSDLLEIFLAVTEGRLXXXXXXXXXXXXVCVVLASEGYPGPYPKGVPIAGLENSPN--D 358 +KSD++ + LAV G L VCVV+AS GYP Y KG I GLE++ D Sbjct: 300 MKSDIVPVLLAVARGDLSGIELEWHDMAAVCVVMASGGYPADYRKGDEIRGLEDAARMED 359 Query: 359 L-VFHAGTERTEEGTWVTNGGRVLGVVGMGXXXXXXXXXXXXXXXKISFPGMHSRSDIAM 417 L VFHAGT R + G VT+GGRVLGV +G IS+ G+H R DI Sbjct: 360 LFVFHAGTSR-KNGRIVTSGGRVLGVTALGATVGEAIDRAYRGVAAISWDGVHYRKDIGA 418 Query: 418 KAL 420 KAL Sbjct: 419 KAL 421 >gb|AAN87361.1| Phosphoribosylamine--glycine ligase [Heliobacillus mobilis] Length = 438 Score = 427 bits (1099), Expect = e-118, Method: Compositional matrix adjust. Identities = 222/428 (51%), Positives = 285/428 (66%), Gaps = 10/428 (2%) Query: 1 MDILVVGGGGREHAIIWSLSRSPKAGKIYCAPGNAGIAQLAECVPIGVFEFDKLTAFAKE 60 + +LVVGGGGREHA++W L +SP+ ++YCAPGNAGI ++A+CV I + L FA E Sbjct: 9 LKVLVVGGGGREHALVWKLRQSPRVSEVYCAPGNAGIEKIAKCVNIKADDVTGLLTFAWE 68 Query: 61 KSIDYVVIGPDDPLAAGIVDAFEAHHIPVFGPRKNAAEIEGSKTFMKDLLHKYSIPTAAY 120 K ID V+GP+ PL+AGIVD F H +P+FGP + AAEIEGSK F KDL+ KY IPTA+Y Sbjct: 69 KRIDLTVVGPEAPLSAGIVDVFNDHEMPIFGPTQKAAEIEGSKAFSKDLMQKYGIPTASY 128 Query: 121 EKFSDYEAALSYLRG-------QGLPVVIKADGLAAGKGVTVAYSREEAELALRDIMVTK 173 F++ E AL+++ G P V+KADGLAAGKGV VA EA+ A+R ++ Sbjct: 129 GVFTEVEPALAFVDELAARKTIAGSPCVVKADGLAAGKGVIVAKDVTEAKEAVRSMLEGN 188 Query: 174 VFGEAGAQVVIEEFLAGQEMSILAFVDGETVRPMAAAQDHKPVFDGDKGPNTGGMGTYSP 233 FG AG++VVIEEF+ G+E+SILAF DG+ V PM +AQDHK +FD D+GPNTGGMG YSP Sbjct: 189 AFGSAGSRVVIEEFMEGEEVSILAFTDGDHVIPMVSAQDHKRIFDNDEGPNTGGMGAYSP 248 Query: 234 LPHIDEAIIQEAVETIIKPTARAMVAEGRPFSGVLFAGLMISPDGKPKTIEFNARFGDPE 293 P E + Q+ E+I+KPT AM AEGRP+SGVL+AGLMI+ +G PK +E+NARFGDPE Sbjct: 249 APVYTEELAQQVQESILKPTVEAMKAEGRPYSGVLYAGLMITAEG-PKVLEYNARFGDPE 307 Query: 294 TQVVLPRLKSDLLEIFLAVTEGRLXXXXXXXXXXXXVCVVLASEGYPGPYPKGVPIAGLE 353 TQ VL RL +DL++I A+ E RL VCVV++++GYPG Y KG I GL+ Sbjct: 308 TQPVLMRLDTDLVDIIEAILEKRLDQQAISWSTDAAVCVVMSAKGYPGDYEKGNFITGLD 367 Query: 354 NSPNDL-VFHAGTERTEEGTWVTNGGRVLGVVGMGXXXXXXXXXXXXXXXKISFPGMHSR 412 N + VFHAGT + + VT GGRVLGV + F G H R Sbjct: 368 NPIAGVEVFHAGTAQKGDHV-VTTGGRVLGVTAKAQGIREAIELAYRQVGNVCFDGAHYR 426 Query: 413 SDIAMKAL 420 DI +AL Sbjct: 427 KDIGRRAL 434 >ref|NP_241500.1| phosphoribosylglycinamide synthetase [Bacillus halodurans] sp|Q9KF52|PUR2_BACHD Phosphoribosylamine--glycine ligase (GARS) (Glycinamide ribonucleotide synthetase) (Phosphoribosylglycinamide synthetase) pir||B83729 phosphoribosylglycinamide synthetase purD [imported] - Bacillus halodurans (strain C-125) dbj|BAB04353.1| phosphoribosylglycinamide synthetase [Bacillus halodurans] Length = 428 Score = 427 bits (1098), Expect = e-118, Method: Compositional matrix adjust. Identities = 218/421 (51%), Positives = 286/421 (67%), Gaps = 5/421 (1%) Query: 1 MDILVVGGGGREHAIIWSLSRSPKAGKIYCAPGNAGIAQLAECVPIGVFEFDKLTAFAKE 60 M++LV+G GGREH I W ++S K ++Y APGN G++ +A CV I + D+L AFAKE Sbjct: 1 MNVLVIGSGGREHTIAWKFAQSEKVERVYVAPGNDGMSDVATCVAISEQDHDQLVAFAKE 60 Query: 61 KSIDYVVIGPDDPLAAGIVDAFEAHHIPVFGPRKNAAEIEGSKTFMKDLLHKYSIPTAAY 120 I +GP+ PL AGIVD F+ + VFGP K AAEIEGSK++ K ++ Y+IPT +Y Sbjct: 61 NKIGLTFVGPEVPLLAGIVDRFQEEGLRVFGPSKRAAEIEGSKSYAKQVMKTYNIPTGSY 120 Query: 121 EKFSDYEAALSYLRGQGLPVVIKADGLAAGKGVTVAYSREEAELALRDIMVTKVFGEAGA 180 E F+ ++ A +Y+ +G+P+VIKADGLAAGKGV VA + EEA AL D++ FG AGA Sbjct: 121 EVFTSFDEAKAYVEAEGVPIVIKADGLAAGKGVVVALTNEEAIAALDDMLNQDKFGGAGA 180 Query: 181 QVVIEEFLAGQEMSILAFVDGETVRPMAAAQDHKPVFDGDKGPNTGGMGTYSPLPHIDEA 240 +VVIEE+L G+E+S++AFV GETV PM AQDHK FDGD+GPNTGGMG YSP+P + Sbjct: 181 RVVIEEYLEGEELSLMAFVHGETVIPMVGAQDHKRAFDGDQGPNTGGMGAYSPVPQFSDV 240 Query: 241 IIQEAVETIIKPTARAMVAEGRPFSGVLFAGLMISPDGKPKTIEFNARFGDPETQVVLPR 300 +++AV I+ PTARA++ E R F+G+L+AGLM++ DG PK IEFNARFGDPETQVVLPR Sbjct: 241 QLKQAVNEILIPTARALMQEERSFTGILYAGLMMTADG-PKVIEFNARFGDPETQVVLPR 299 Query: 301 LKSDLLEIFLAVTEGRLXXXXXXXXXXXXVCVVLASEGYPGPYPKGVPIAGLENSPND-L 359 LKSDL+ + ++ +G+ + VVLA++GYPG Y KG I+GLE +D L Sbjct: 300 LKSDLVNVIESLLDGQ--EPELEWDEQAVLGVVLATKGYPGSYEKGYTISGLEQLEDDTL 357 Query: 360 VFHAGTERTEEGTWVTNGGRVLGVVGMGXXXXXXXXXXXXXXXKISFPGMHSRSDIAMKA 419 VFHAGT+R EE VTNGGRVL V K+ G+ R DI KA Sbjct: 358 VFHAGTKREEE-ELVTNGGRVLLVAKQASTLREAQAAVYEELNKVKSDGLFYRKDIGSKA 416 Query: 420 L 420 + Sbjct: 417 I 417 >ref|NP_830173.1| Phosphoribosylamine--glycine ligase [Bacillus cereus ATCC 14579] gb|AAP07374.1| Phosphoribosylamine--glycine ligase [Bacillus cereus ATCC 14579] Length = 423 Score = 422 bits (1086), Expect = e-117, Method: Compositional matrix adjust. Identities = 218/420 (51%), Positives = 286/420 (68%), Gaps = 4/420 (0%) Query: 1 MDILVVGGGGREHAIIWSLSRSPKAGKIYCAPGNAGIAQLAECVPIGVFEFDKLTAFAKE 60 M++LV+G GGREHA+ W ++S K K+Y APGN G+ +A V I +FD L FAKE Sbjct: 1 MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPVDIDENDFDALVLFAKE 60 Query: 61 KSIDYVVIGPDDPLAAGIVDAFEAHHIPVFGPRKNAAEIEGSKTFMKDLLHKYSIPTAAY 120 +++ +GP+ PL GIVD F+ + VFGP K AA IEGSK F K+L+ KY IPTAAY Sbjct: 61 NNVELTFVGPEIPLMNGIVDCFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYDIPTAAY 120 Query: 121 EKFSDYEAALSYLRGQGLPVVIKADGLAAGKGVTVAYSREEAELALRDIMVTKVFGEAGA 180 E F+DYE A+ Y++ G P+VIKADGLAAGKGVTVA + EEA A+++++ FG A Sbjct: 121 ETFTDYEEAVQYIQKVGAPIVIKADGLAAGKGVTVAMTLEEALQAVKEMLQDVKFGAASK 180 Query: 181 QVVIEEFLAGQEMSILAFVDGETVRPMAAAQDHKPVFDGDKGPNTGGMGTYSPLPHIDEA 240 ++VIEEFL GQE S++AFV+G TV PM AQDHK FDGDKGPNTGGMG YSP+P I E+ Sbjct: 181 KIVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQIPES 240 Query: 241 IIQEAVETIIKPTARAMVAEGRPFSGVLFAGLMISPDGKPKTIEFNARFGDPETQVVLPR 300 +QEA++T++ PTA+AM+AE R F+G+L+AGL+++ DG PK IEFNARFGDPET+VVLPR Sbjct: 241 AVQEAIQTVLHPTAKAMIAENRSFTGILYAGLILTNDG-PKVIEFNARFGDPETEVVLPR 299 Query: 301 LKSDLLEIFLAVTEGRLXXXXXXXXXXXXVCVVLASEGYPGPYPKGVPIAGLENSPNDLV 360 L++DL+++ AV + + VVLAS+GYP Y KG I+GL+ + +V Sbjct: 300 LENDLVDVCNAVLDES--ELTLQWSEEAVIGVVLASKGYPEAYKKGDIISGLDALQDVIV 357 Query: 361 FHAGTERTEEGTWVTNGGRVLGVVGMGXXXXXXXXXXXXXXXKISFPGMHSRSDIAMKAL 420 FHAGT + G +VTNGGRVL V KI G+ RSDI +A+ Sbjct: 358 FHAGT-AMKHGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKIESDGLFYRSDIGYRAI 416 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 617,614,603 Number of extensions: 26233870 Number of successful extensions: 60982 Number of sequences better than 1.0e-05: 843 Number of HSP's gapped: 59508 Number of HSP's successfully gapped: 845 Length of query: 421 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 293 Effective length of database: 409,704,906 Effective search space: 120043537458 Effective search space used: 120043537458 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)