BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0440.0009.p (311 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00046604.1| hypothetical protein [Lactobacillus gasseri] 307 2e-82 ref|NP_965723.1| hypothetical protein LJ0532 [Lactobacillus john... 293 4e-78 ref|ZP_00037189.1| hypothetical protein [Enterococcus faecium] 280 2e-74 ref|NP_814516.1| conserved hypothetical protein [Enterococcus fa... 276 6e-73 ref|ZP_00035423.1| hypothetical protein [Enterococcus faecium] 256 7e-67 >ref|ZP_00046604.1| hypothetical protein [Lactobacillus gasseri] Length = 299 Score = 307 bits (786), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 158/309 (51%), Positives = 205/309 (66%), Gaps = 11/309 (3%) Query: 4 MTKRLLNCNASDLLAMNRDELKLSIYASEGRTILAETAVVKEPIIDGITNGEIARFAGAD 63 M R+++ NAS++L MN ELK SI ASEGRT+L+E V+ EP IDG+T EIA GAD Sbjct: 1 MVTRIISANASEILKMNGAELKQSIKASEGRTVLSENVVI-EPAIDGLTTSEIAAAFGAD 59 Query: 64 LLLLNAFDLEQPQISGIDPADPQ-PVRTLRNLIGRPVGANLEPVDDSRETMDEKEHLGPG 122 L+LLN FD P++SG+ P+ V+ L+ L GRP+G NLEPVD+S E K + G Sbjct: 60 LILLNLFDTLNPKVSGLTVDKPEDTVKQLQKLTGRPIGVNLEPVDESAEMESTKLQISAG 119 Query: 123 RRATVSTFLQANELGLDFICLTGNPGTGVTNSAICHSISQARQHFHGLIIAGKMHSSGID 182 R ATV + A +LG +F+C TGNPGTGVTN I ++ A+++F GLIIAGKMH +G+D Sbjct: 120 RTATVESMKAAEKLGFNFVCFTGNPGTGVTNKMIAKAVKTAKENFSGLIIAGKMHGAGVD 179 Query: 183 EPIIDAETVQAFIDAGADIILFPAVYTVPKFREEDLTRMVEIVHTHNRSVTDPSKKVLTL 242 EP+ + E+V+AF+DAGAD+IL PAV TVP F E+L ++V+IVH H L + Sbjct: 180 EPVANEESVKAFLDAGADVILVPAVGTVPGFTSEELIKIVKIVHEHGG---------LVM 230 Query: 243 SAIGTSQESSSKEVVQKIALACKACGVDIQHIGDAGFSGLALYQNIDALGNAIRGERHQL 302 S IGTSQESS + V IAL K CGVDIQHIGDAG+ + + I L IRG H + Sbjct: 231 STIGTSQESSGAQTVHDIALQNKICGVDIQHIGDAGWGVVPPTETIFELSKTIRGMNHTI 290 Query: 303 RMRAKSIIR 311 A+SI R Sbjct: 291 SRMARSINR 299 >ref|NP_965723.1| hypothetical protein LJ0532 [Lactobacillus johnsonii NCC 533] gb|AAS09689.1| hypothetical protein LJ0532 [Lactobacillus johnsonii NCC 533] Length = 299 Score = 293 bits (750), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 152/309 (49%), Positives = 198/309 (64%), Gaps = 11/309 (3%) Query: 4 MTKRLLNCNASDLLAMNRDELKLSIYASEGRTILAETAVVKEPIIDGITNGEIARFAGAD 63 M R+++ N S++L M+ +LK SI ASEGRT+L+E VV EP ID +T EIA GAD Sbjct: 1 MVTRIISANTSEILKMDGTQLKQSIKASEGRTVLSEN-VVTEPAIDNLTTSEIAAAFGAD 59 Query: 64 LLLLNAFDLEQPQISGIDPADPQ-PVRTLRNLIGRPVGANLEPVDDSRETMDEKEHLGPG 122 L+LLN FD P++SG++ P+ V+ L+ L GRP+G NLEPVD+ E K + G Sbjct: 60 LILLNLFDTLNPKVSGLEVDKPENTVKKLQKLTGRPIGVNLEPVDEKAEMESTKLQISSG 119 Query: 123 RRATVSTFLQANELGLDFICLTGNPGTGVTNSAICHSISQARQHFHGLIIAGKMHSSGID 182 R A V + A ELG +F+C TGNPGTGVTN I ++ A+++F GLIIAGKMH +G+D Sbjct: 120 RTANVESMRAAEELGFNFVCFTGNPGTGVTNRMIAKAVKTAKKNFSGLIIAGKMHGAGVD 179 Query: 183 EPIIDAETVQAFIDAGADIILFPAVYTVPKFREEDLTRMVEIVHTHNRSVTDPSKKVLTL 242 EP+ E+V+ F+DAGAD+IL PAV TVP F E+L +V+IVH H L + Sbjct: 180 EPVASEESVKEFLDAGADVILVPAVGTVPGFTSEELINIVKIVHKHGG---------LVM 230 Query: 243 SAIGTSQESSSKEVVQKIALACKACGVDIQHIGDAGFSGLALYQNIDALGNAIRGERHQL 302 S IGTSQESS + V IAL K CG+DIQHIGDAG+ + I L IRG H + Sbjct: 231 STIGTSQESSGAQTVHDIALQNKICGIDIQHIGDAGWGVVPPTDTIFELSKTIRGMNHTI 290 Query: 303 RMRAKSIIR 311 A+SI R Sbjct: 291 SRMARSINR 299 >ref|ZP_00037189.1| hypothetical protein [Enterococcus faecium] Length = 310 Score = 280 bits (717), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 146/319 (45%), Positives = 201/319 (63%), Gaps = 20/319 (6%) Query: 4 MTKRLLNCNASDLLAMNRDELKLSIYASEGRTILAETAVVKEPIIDGITNGEIARFAGAD 63 M KRL++ N SD+ M ELK SI ASEGR I+ ET P ITN E+A GAD Sbjct: 1 MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQSGEITNAEVASAFGAD 60 Query: 64 LLLLNAFDLEQPQISGIDPADPQ-----------PVRTLRNLIGRPVGANLEPVDDSRET 112 L+LLN FD +P + G+ + P+ L+ L+GRPVG NLEP+ Sbjct: 61 LILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKELVGRPVGINLEPIAPKTNM 120 Query: 113 MDEKEHLGPGRRATVSTFLQANELGLDFICLTGNPGTGVTNSAICHSISQARQHFHGLII 172 M EK + GR ++V T +A ELG+DFIC TGNPGTGVTN I S+ A+++F GLII Sbjct: 121 MSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVTNLEITKSVKMAKKYFSGLII 180 Query: 173 AGKMHSSGIDEPIIDAETVQAFIDAGADIILFPAVYTVPKFREEDLTRMVEIVHTHNRSV 232 AGKMHS+G +EP+++ E + ++ +AGADI+L P+V ++P F E+DL V + ++ Sbjct: 181 AGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSIPGFLEQDLIEAVRLAKKYD--- 237 Query: 233 TDPSKKVLTLSAIGTSQESSSKEVVQKIALACKACGVDIQHIGDAGFSGLALYQNIDALG 292 +LT+SAIGTSQES+ E ++++A+ K+CGVD+QHIGDAG+ GLA +NI Sbjct: 238 ------LLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDAGYGGLAPAENIFETS 291 Query: 293 NAIRGERHQLRMRAKSIIR 311 AIRG RH + ++SI R Sbjct: 292 VAIRGMRHTINCVSRSINR 310 >ref|NP_814516.1| conserved hypothetical protein [Enterococcus faecalis V583] gb|AAO80586.1| conserved hypothetical protein [Enterococcus faecalis V583] Length = 307 Score = 276 bits (705), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 148/318 (46%), Positives = 207/318 (65%), Gaps = 21/318 (6%) Query: 4 MTKRLLNCNASDLLAMNRDELKLSIYASEGRTILAETAVVKEPIIDGITNGEIARFAGAD 63 M +RL++ NAS++ M+++EL SI ASEGR IL+E VV + IT E+++ GAD Sbjct: 1 MVQRLISANASEIKRMSKEELFQSIKASEGRVILSENVVVHPSVAGDITCAEMSKAYGAD 60 Query: 64 LLLLNAFDLEQPQISGID----------PADPQPVRTLRNLIGRPVGANLEPVDDSRETM 113 ++LLN FD+ QP + P D + V L+ L+G P+G N+EPVD++ + Sbjct: 61 MILLNVFDVNQPIVVAAYEGQYTAETCVPND-EVVHRLKELVGLPIGMNVEPVDENLDLA 119 Query: 114 DEKEHLGPGRRATVSTFLQANELGLDFICLTGNPGTGVTNSAICHSISQARQHFHGLIIA 173 + + PGR+A+ +TF +ANELGLDFI LTGNPGTGVTN I +++ A++HF G+IIA Sbjct: 120 STRVSIEPGRKASAATFKKANELGLDFILLTGNPGTGVTNDLIAKNVALAKKHFDGIIIA 179 Query: 174 GKMHSSGIDEPIIDAETVQAFIDAGADIILFPAVYTVPKFREEDLTRMVEIVHTHNRSVT 233 GKMHSSG+DEP++ ++ + FI+AGADI+L PAV TV ++ + +V+ H Sbjct: 180 GKMHSSGVDEPVVSLKSAEQFIEAGADIVLVPAVGTVWGIDDQQVKEVVDFAH------- 232 Query: 234 DPSKKVLTLSAIGTSQESSSKEVVQKIALACKACGVDIQHIGDAGFSGLALYQNIDALGN 293 SK L +SAIGTSQES+ EV+Q I + K GVDIQHIGDA GL +NI L + Sbjct: 233 --SKGKLVMSAIGTSQESAQPEVIQAIGIRNKILGVDIQHIGDANM-GLVGIENIKELSD 289 Query: 294 AIRGERHQLRMRAKSIIR 311 AIRG+RH + A+SI R Sbjct: 290 AIRGKRHTVARMARSINR 307 >ref|ZP_00035423.1| hypothetical protein [Enterococcus faecium] Length = 275 Score = 256 bits (653), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 132/262 (50%), Positives = 175/262 (66%), Gaps = 3/262 (1%) Query: 51 ITNGEIARFAGADLLLLNAFDLEQPQISGID-PADPQPVRTLRNLIGRPVGANLEPVDDS 109 +TN E+ R AGADL+L NA DL PQISG+ P+ ++ +GR +G NLEPVD Sbjct: 16 VTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNLEPVDKE 75 Query: 110 RETMDEKEHLGPGRRATVSTFLQANELGLDFICLTGNPGTGVTNSAICHSISQARQHFHG 169 + + + GR + T +ANELG +FICLTGNPGTGV+N I +I+ ++ F+G Sbjct: 76 VLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAITLSKSIFNG 135 Query: 170 LIIAGKMHSSGIDEPIIDAETVQAFIDAGADIILFPAVYTVPKFREEDLTRMVEIVHTHN 229 LIIAGKMH +G+DEPI++ + + FI G DI+L PA YTVP F E DL +V+ + N Sbjct: 136 LIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLRGIVDYIKDFN 195 Query: 230 RSVTDPSKKVLTLSAIGTSQESSSKEVVQKIALACKACGVDIQHIGDAGFSGLALYQNID 289 R+ +KVL L+A GTSQ+SS +E ++KIALA KACG DIQHIGD+ +G+ L +NI Sbjct: 196 RN-KSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDS-LNGICLPENIF 253 Query: 290 ALGNAIRGERHQLRMRAKSIIR 311 ALG AIRG RHQ+ M KS R Sbjct: 254 ALGQAIRGYRHQIIMLGKSNYR 275 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 430,517,275 Number of extensions: 17794236 Number of successful extensions: 47744 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 47715 Number of HSP's successfully gapped: 11 Length of query: 311 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 186 Effective length of database: 417,850,203 Effective search space: 77720137758 Effective search space used: 77720137758 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)