BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0438.0002.p (172 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00130228.1| COG2200: FOG: EAL domain [Desulfovibrio desul... 136 2e-31 gb|EAJ80691.1| unknown [environmental sequence] 126 2e-28 gb|EAK51810.1| unknown [environmental sequence] 126 2e-28 ref|ZP_00136611.1| COG2200: FOG: EAL domain [Pseudomonas aerugin... 124 7e-28 ref|NP_251948.1| hypothetical protein [Pseudomonas aeruginosa PA... 124 9e-28 >ref|ZP_00130228.1| COG2200: FOG: EAL domain [Desulfovibrio desulfuricans G20] Length = 766 Score = 136 bits (343), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 63/143 (44%), Positives = 94/143 (65%) Query: 1 VLNDSRHKSGETLSLLQELNLSPERVVFELTERQAVQDYVGFEKVLSHYREQGFRIAVDD 60 L D +G T +L+ LSP+ VV E+TER +++++ F K L+HYR QG++IA+DD Sbjct: 265 TLTDPAFSTGNTRKMLEAAGLSPQNVVLEITERHSIRNFTLFYKTLAHYRGQGYKIAIDD 324 Query: 61 AGSGYNSLKTLVYLKPEFIKLDKFLIRGIHSNREQQELLELISEYASRSATRVIAEGIET 120 AG+GY+ L ++ + P+FIK+D LIRGI+ + ++ L+E + A + +RVIAEGIET Sbjct: 325 AGTGYSGLSSIAEITPDFIKVDMSLIRGINRDPVRRALMETMISLADKIGSRVIAEGIET 384 Query: 121 EEELLYLQMASIEFGQGYALGRP 143 EE + L FGQG+ GRP Sbjct: 385 REEAMTLAETGAHFGQGFYFGRP 407 >gb|EAJ80691.1| unknown [environmental sequence] Length = 393 Score = 126 bits (316), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 65/146 (44%), Positives = 87/146 (59%) Query: 2 LNDSRHKSGETLSLLQELNLSPERVVFELTERQAVQDYVGFEKVLSHYREQGFRIAVDDA 61 L D H G TL LLQ+L +SP +VV EL+E+ D + L+HYR QGF A+DD Sbjct: 98 LLDPTHPKGMTLQLLQQLGISPSQVVIELSEQYPADDIDLLKSCLNHYRSQGFMTAIDDL 157 Query: 62 GSGYNSLKTLVYLKPEFIKLDKFLIRGIHSNREQQELLELISEYASRSATRVIAEGIETE 121 G+GY+ L+ L P+++K+D+ I I S +QE + I E +VIAEGIET+ Sbjct: 158 GAGYSGLRLWSELSPDYVKIDRHFIHQIDSTPVKQEFVRSIVELCQSLTCKVIAEGIETQ 217 Query: 122 EELLYLQMASIEFGQGYALGRPGYLP 147 EEL L+ I + QGY LGRP LP Sbjct: 218 EELAVLKQLGIVYCQGYLLGRPEALP 243 >gb|EAK51810.1| unknown [environmental sequence] Length = 584 Score = 126 bits (316), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 65/146 (44%), Positives = 87/146 (59%) Query: 2 LNDSRHKSGETLSLLQELNLSPERVVFELTERQAVQDYVGFEKVLSHYREQGFRIAVDDA 61 L D H G TL LLQ+L +SP +VV EL+E+ D + L+HYR QGF A+DD Sbjct: 98 LLDPAHPKGMTLQLLQQLGISPSQVVIELSEQYPADDIDLLKSCLNHYRSQGFMTAIDDL 157 Query: 62 GSGYNSLKTLVYLKPEFIKLDKFLIRGIHSNREQQELLELISEYASRSATRVIAEGIETE 121 G+GY+ L+ L P+++K+D+ I I S +QE + I E +VIAEGIET+ Sbjct: 158 GAGYSGLRLWSELSPDYVKIDRHFIHQIDSTPVKQEFVRSIVELCQSLTCKVIAEGIETQ 217 Query: 122 EELLYLQMASIEFGQGYALGRPGYLP 147 EEL L+ I + QGY LGRP LP Sbjct: 218 EELAVLKQLGIVYCQGYLLGRPEALP 243 >ref|ZP_00136611.1| COG2200: FOG: EAL domain [Pseudomonas aeruginosa UCBPP-PA14] Length = 582 Score = 124 bits (312), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 68/164 (41%), Positives = 94/164 (57%), Gaps = 5/164 (3%) Query: 7 HKSGETLSLLQELNLSPERVVFELTERQAVQDYVGFEKVLSHYREQGFRIAVDDAGSGYN 66 H G TL LLQ+L +SP RVV ELTE+ + D+ + L HYR GF IA+DD G+GY+ Sbjct: 103 HPPGRTLQLLQQLGISPNRVVIELTEQTPIDDFDLLDTALHHYRAMGFSIALDDLGAGYS 162 Query: 67 SLKTLVYLKPEFIKLDKFLIRGIHSNREQQELLELISEYASRSATRVIAEGIETEEELLY 126 SL+ L+P+++K+D+ + GIH + ++E + I + A S +VIAEGIE EEL Sbjct: 163 SLRLWSELRPDYVKIDRHFVDGIHLDTVKREFVGSILKMARASRAQVIAEGIELPEELAV 222 Query: 127 LQMASIEFGQGYALGRPGYLPEPGAFPSDSPQVSAGGGCKHVSS 170 L ++ QGY GRP P SD Q+ G SS Sbjct: 223 LSEMGVDLVQGYLFGRPQEHPA-----SDVQQMLPSAGAPSQSS 261 >ref|NP_251948.1| hypothetical protein [Pseudomonas aeruginosa PA01] pir||D83238 hypothetical protein PA3258 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG06646.1| hypothetical protein PA3258 [Pseudomonas aeruginosa PAO1] Length = 601 Score = 124 bits (311), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 62/141 (43%), Positives = 87/141 (61%) Query: 7 HKSGETLSLLQELNLSPERVVFELTERQAVQDYVGFEKVLSHYREQGFRIAVDDAGSGYN 66 H G TL LLQ+L +SP RVV ELTE+ + D+ + L HYR GF IA+DD G+GY+ Sbjct: 122 HPPGRTLQLLQQLGISPNRVVIELTEQTPIDDFDLLDTALHHYRSMGFSIALDDLGAGYS 181 Query: 67 SLKTLVYLKPEFIKLDKFLIRGIHSNREQQELLELISEYASRSATRVIAEGIETEEELLY 126 SL+ L+P+++K+D+ + GIH + ++E + I + A S +VIAEGIE EEL Sbjct: 182 SLRLWSELRPDYVKIDRHFVDGIHLDTVKREFVGSILKMARASRAQVIAEGIELPEELAV 241 Query: 127 LQMASIEFGQGYALGRPGYLP 147 L ++ QGY GRP P Sbjct: 242 LSEMGVDLVQGYLFGRPQEHP 262 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 253,816,877 Number of extensions: 9887696 Number of successful extensions: 21769 Number of sequences better than 1.0e-05: 1183 Number of HSP's gapped: 21581 Number of HSP's successfully gapped: 1183 Length of query: 172 Length of database: 757,237,578 Length adjustment: 117 Effective length of query: 55 Effective length of database: 439,570,995 Effective search space: 24176404725 Effective search space used: 24176404725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 121 (51.2 bits)