BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0437.0028.p (531 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00059682.1| COG4753: Response regulator containing CheY-l... 130 6e-29 ref|ZP_00180754.1| hypothetical protein Mther123301 [Moorella th... 94 1e-17 ref|NP_243594.1| two-component response regulator [Bacillus halo... 92 2e-17 ref|NP_782430.1| transcriptional regulatory protein [Clostridium... 92 2e-17 ref|ZP_00181951.1| hypothetical protein Exigu277201 [Exiguobacte... 92 4e-17 >ref|ZP_00059682.1| COG4753: Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain [Clostridium thermocellum ATCC 27405] Length = 540 Score = 130 bits (328), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 130/525 (24%), Positives = 232/525 (44%), Gaps = 45/525 (8%) Query: 27 DQVAVAKDGLAAM-ELAKSHRPEAILTDIRMPGMDGLEFLEWLKLEGIKAKVVMVSAYNL 85 D + AKDG+ A+ +L + ++ + +++DI +P M+G+E L +K + + +V++S N Sbjct: 25 DFICSAKDGIDALNKLNQHNKLDLVISDIDIPKMNGIELLNSIKEYNLSSMIVLLSDENN 84 Query: 86 FEYAQTAVRHGAYDYLLKPVDVQKIEEVLSRIDVQLNXXXXXXXXXXXXXXXXXXSSCAY 145 + + + GA+DY+LKP+D K+ +VL + L + Sbjct: 85 MAHVKQGLLLGAFDYILKPLDKDKVIDVLKKAHDSLMDKKMEEERNKNLKKKLELNLSLS 144 Query: 146 RNRLILAWLNGSA-TVPELEEL-NRYEWLRESGVVVYSELESFREGAQPQDSDQLVS--R 201 R++++ L G EL+ + N Y + G+V +E G DS +L+ R Sbjct: 145 RDKILQDLLRGKEFPFQELDYIQNEYNISLQKGMVQVGIIEI---GNFDADSRELIKSGR 201 Query: 202 LEQVWSQWGEALTVPLSGTLE---DGRQAAVTLVSLRRLDGEQREKGRXXXXXXXSDWAH 258 + + + G+ ++ LS E + + + L+S+ Q+E +D+ Sbjct: 202 FDSLVEEVGKIISNTLSEFPELNCNMVEMDIGLISVILQPVGQKE--LQDFEDMTADFFE 259 Query: 259 ----------TGRLAHGIGPASPSLLAGAPQAYLSARMAYSYNFYEGWKGLVFL----DE 304 R GIG A SL Q+Y+ A+ A + F G ++ + +E Sbjct: 260 KVLKGIKQDANMRATIGIGGAYSSL-RDISQSYMGAKAALRHKFILGGNRVIHIKTDYNE 318 Query: 305 ISSSPLAASPDWGRLYEALKGDDAALVLHVCGEIFTQLAGG--------GHAVPMLLKEK 356 + A + + + GDD AL L +F +A G A L+ Sbjct: 319 KQNLLYPAEREKMLVESIMSGDDNALKL--AENMFDDIAIGTGDNLKRIAFAANQLVFNI 376 Query: 357 ASLMLLQIRSDNQDILDRKAGQMLADTATRLIRSCSSYKELMARLAKALQEVQLALSLSR 416 ++ + +Q N+ RK M SS E+ + E+ + + Sbjct: 377 SNFIDMQYDFINKLYDFRKFNNMD-------FSKFSSKDEIKEFFLSFVTELLNVVKEYK 429 Query: 417 QDQGEVVITECLSWIREHTKEEVTLERAADYFHFNPSYFSTLFKSRTGRTFSEHVTAVRM 476 Q +I + ++ H +E+TL ADY + + +YF +LFK TG F E+VT V+M Sbjct: 430 PAQNNTLIKKACEYVLNHIDQEITLMTIADYLNISKNYFCSLFKQETGYNFLEYVTKVKM 489 Query: 477 KRAKELLAEDKLRIYEISVECGFQDPKYFCRVFKKYHSMSPKTYK 521 + AK+LL E + YE+S G+++ YF R+F+KY +SP YK Sbjct: 490 EWAKKLLREGNYKTYEVSEMLGYREASYFSRLFRKYTKLSPAEYK 534 >ref|ZP_00180754.1| hypothetical protein Mther123301 [Moorella thermoacetica ATCC 39073] Length = 439 Score = 93.6 bits (231), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 64/250 (25%), Positives = 119/250 (47%), Gaps = 5/250 (2%) Query: 279 QAYLSARMAYSYNFYEGWKGLVFLDEISSSPLAA----SPDWGRLYEALKGDDAALVLHV 334 ++Y A A + + G ++ D++ + P A +P+ L +A++ D Sbjct: 180 RSYAEAVAAAEFRLFYGGDQVIHADDVIARPSAGQFLPAPEEQELTQAIRMGDRQAAYRQ 239 Query: 335 CGEIFTQLAGGGHAVPMLLKEKA-SLMLLQIRSDNQDILDRKAGQMLADTATRLIRSCSS 393 I QL P +LK K L L R+ + D +A LA ++ + + Sbjct: 240 AKNILMQLLLEQEKRPAILKMKLLELNTLAARAALEGGADPEAVSDLALASSTEFLTLDN 299 Query: 394 YKELMARLAKALQEVQLALSLSRQDQGEVVITECLSWIREHTKEEVTLERAADYFHFNPS 453 ++ R+ + L + ++ +R+ + +I +I + +++TLE A + +P Sbjct: 300 LADMRERILERLMALVAQVAETREQRNSSLIDRASKYIEANFSQDLTLEEVARQVYLSPC 359 Query: 454 YFSTLFKSRTGRTFSEHVTAVRMKRAKELLAEDKLRIYEISVECGFQDPKYFCRVFKKYH 513 YFS LFK G F +++T VR+K A+ELL KL + EI+ G++D +YF +VFKK Sbjct: 360 YFSKLFKQFKGLNFIDYLTKVRLKAARELLLNTKLPVAEIATRVGYRDARYFGQVFKKQE 419 Query: 514 SMSPKTYKHV 523 +P ++ + Sbjct: 420 GYTPSVFRKI 429 >ref|NP_243594.1| two-component response regulator [Bacillus halodurans] pir||H83990 two-component response regulator BH2728 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06447.1| two-component response regulator [Bacillus halodurans] Length = 510 Score = 92.4 bits (228), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 43/104 (41%), Positives = 62/104 (59%) Query: 418 DQGEVVITECLSWIREHTKEEVTLERAADYFHFNPSYFSTLFKSRTGRTFSEHVTAVRMK 477 ++G I + L++I EH E ++L + A+YFHFNPSY S F+ FSE++ VR++ Sbjct: 396 NEGSSSIQKMLAYIEEHHSEPLSLSQLANYFHFNPSYLSAYFRQHHDEGFSEYLNKVRIR 455 Query: 478 RAKELLAEDKLRIYEISVECGFQDPKYFCRVFKKYHSMSPKTYK 521 +AKE L + I EI G+ D YFC+VFKK M P Y+ Sbjct: 456 KAKEWLKSSTIPISEIYALVGYSDHSYFCKVFKKRTGMPPSRYR 499 Score = 69.7 bits (169), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 35/104 (33%), Positives = 62/104 (59%), Gaps = 1/104 (0%) Query: 2 RILIVDDEPRHLRGMVNLIHRLRPEDQ-VAVAKDGLAAMELAKSHRPEAILTDIRMPGMD 60 +LIVDDE +G++N + Q V A +G A+EL + P ++TDI MP MD Sbjct: 3 NVLIVDDEKLIRQGIINFFDWEKEGFQIVGEAANGKDALELISKNEPHIVMTDIVMPVMD 62 Query: 61 GLEFLEWLKLEGIKAKVVMVSAYNLFEYAQTAVRHGAYDYLLKP 104 G++ ++ +K + + +++++S++ FEY ++ G DY+LKP Sbjct: 63 GIDLVKRVKRDFPEVEIIVLSSFEEFEYVRSTFHSGVADYILKP 106 >ref|NP_782430.1| transcriptional regulatory protein [Clostridium tetani E88] gb|AAO36367.1| transcriptional regulatory protein [Clostridium tetani E88] Length = 259 Score = 92.4 bits (228), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 45/114 (39%), Positives = 71/114 (62%), Gaps = 1/114 (0%) Query: 2 RILIVDDEPRHLRGMVNLIHRLRPE-DQVAVAKDGLAAMELAKSHRPEAILTDIRMPGMD 60 RI+I DDE G+ NLI +A AKDG AM++ + + PE IL DI MP M+ Sbjct: 3 RIVIADDEDTIRNGLKNLIESYELNLSVIATAKDGEEAMKVIEKYHPEIILMDINMPFMN 62 Query: 61 GLEFLEWLKLEGIKAKVVMVSAYNLFEYAQTAVRHGAYDYLLKPVDVQKIEEVL 114 GL+ ++ + AK++++S Y+ FEYAQ A+ G ++YLLKP++ + +++L Sbjct: 63 GLDVIKGIHERNKDAKIIIISGYSQFEYAQKALELGVFNYLLKPINYRDFKDIL 116 Score = 69.3 bits (168), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 42/139 (30%), Positives = 80/139 (57%), Gaps = 9/139 (6%) Query: 387 LIRSCSSYKELMARLAKALQEVQLALSLSRQDQGEVVITECLSWIREH-TKEEVTLERAA 445 LI++ SY + M ++K ++E + + S +D G + I S+I+++ + E++L A Sbjct: 116 LIKAMDSYSKRMWEISK-IKEGKEKIE-SCKDVGNLAI----SYIKKNFSNNEISLNLVA 169 Query: 446 DYFHFNPSYFSTLFKSRTGRTFSEHVTAVRMKRAKELLAEDK--LRIYEISVECGFQDPK 503 F+ + SY + + K +TG +F++++ +R+ AK+LL ++ I +IS G+ Sbjct: 170 QQFYISQSYLTRVIKQKTGVSFTDYLNKLRINMAKKLLTDNNNDYTINDISNMVGYSSQH 229 Query: 504 YFCRVFKKYHSMSPKTYKH 522 YF R FK Y +SPK Y++ Sbjct: 230 YFSRAFKNYMEISPKNYRN 248 >ref|ZP_00181951.1| hypothetical protein Exigu277201 [Exiguobacterium sp. 255-15] Length = 116 Score = 91.7 bits (226), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 45/109 (41%), Positives = 67/109 (61%), Gaps = 1/109 (0%) Query: 417 QDQGEVVITECLSWIREHTKEEVTLERAADYFHFNPSYFSTLFKSRTGRTFSEHVTAVRM 476 Q+QGE I + +I H EE+TL+ ++ F+ + Y S FK R S+++ +R+ Sbjct: 8 QEQGEA-IDQIAKYISTHYAEELTLQLMSERFYLSREYISRRFKQRFSVNLSDYIQTIRL 66 Query: 477 KRAKELLAEDKLRIYEISVECGFQDPKYFCRVFKKYHSMSPKTYKHVLS 525 RAKELL E RIYEI++E G+ D KYF +VFKK M+P ++ +LS Sbjct: 67 NRAKELLGETDARIYEIALEVGYHDDKYFRKVFKKQFGMTPNEFRELLS 115 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 716,621,921 Number of extensions: 26770716 Number of successful extensions: 79646 Number of sequences better than 1.0e-05: 1635 Number of HSP's gapped: 81301 Number of HSP's successfully gapped: 1681 Length of query: 531 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 401 Effective length of database: 404,274,708 Effective search space: 162114157908 Effective search space used: 162114157908 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)