BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0430.0006.p (699 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_623945.1| S-layer homology domain [Thermoanaerobacter ten... 139 3e-31 gb|AAD09354.1| s-layer associated multidomain endoglucanase [The... 137 8e-31 ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella th... 134 7e-30 gb|AAB00841.1| pullulanase [Thermoanaerobacterium thermosulfurig... 134 9e-30 sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pu... 134 1e-29 >ref|NP_623945.1| S-layer homology domain [Thermoanaerobacter tengcongensis] gb|AAM25549.1| S-layer homology domain [Thermoanaerobacter tengcongensis] Length = 1321 Score = 139 bits (349), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 80/236 (33%), Positives = 121/236 (51%), Gaps = 11/236 (4%) Query: 466 KLGIYSYNPDKGIWSYIGGVTAAEDGFVQAKISASGLYAVMLREAQFTDI-SGHWAQQDV 524 K+G+Y N G W Y+GG V A++ Y + QF D+ WA + Sbjct: 1095 KVGVYYLNEATGKWEYVGGKVDKNTNTVTAEVKHFSTYGAFEYDKQFKDVPKDFWAYDVI 1154 Query: 525 EVLAARGIVNGMNGTVFAPKAVLTRAEFTKLLTAALGLQPVHTDKPTFRDVSAAHWSYPY 584 VLA++ I+ GM+ FAP A +TRAEF L+ ALG+ P K F DV A W Sbjct: 1155 NVLASKHIIKGMDDDNFAPNAKITRAEFAALMIRALGI-PEEPYKGEFEDVKAGAWYANA 1213 Query: 585 VEXXXXXGIVTGDAGLFHGNAPLTREQMMVMLMRAIDSTTAHSPASAADSVKLQAVLSGF 644 +E GI+ GD + P+TRE+M ++MR T + + ++V F Sbjct: 1214 IEAAYRAGIMMGDGKKMRPDDPITREEMAAVIMRVYAKMTGYKEENIGNTV--------F 1265 Query: 645 KDNGTISQWARESVAAAVDKKLVQG-SGQFLKPKDSSTRAEAAVIMYKLLAELKLL 699 KDN +IS+WAR VA A +V+G + K+++TRAE ++Y++L +L++L Sbjct: 1266 KDNDSISEWARNVVANAAKLGIVKGYEDNTFRAKNNATRAEGVTLLYRILEKLQVL 1321 >gb|AAD09354.1| s-layer associated multidomain endoglucanase [Thermoanaerobacterium polysaccharolyticum] Length = 1097 Score = 137 bits (345), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 69/187 (36%), Positives = 109/187 (58%), Gaps = 5/187 (2%) Query: 511 QFTDISGHWAQQDVEVLAARGIVNGMNGTVFAPKAVLTRAEFTKLLTAALGLQPVHTDKP 570 +FTDIS WA+ +++VLA++ I++G F P +TRAEF +L ALG++ D P Sbjct: 912 KFTDISSSWAKNEIQVLASKNIISGYPDGTFKPDKRITRAEFVSMLVKALGIKQSIPDVP 971 Query: 571 TFRDVSAAHWSYPYVEXXXXXGIVTGDAGLFHGNAPLTREQMMVMLMRAIDSTTAHSPAS 630 TF DV+ W Y VE GI +G F + +TR++MMVM++ A+ S Sbjct: 972 TFSDVNKGDWYYGLVEAAKSTGIASGYGKQFKPDMQITRQEMMVMVVNALRVNKVEKFVS 1031 Query: 631 AADSVKLQAVLSGFKDNGTISQWARESVAAAVDKKLVQGSG-QFLKPKDSSTRAEAAVIM 689 D +VL FKD G + WA++++A V L++G+G ++L P +TRA+AA ++ Sbjct: 1032 KGDV----SVLGKFKDGGKVQNWAKDAMAIGVSNGLIKGTGDEYLSPDGRATRAQAAAVI 1087 Query: 690 YKLLAEL 696 Y++L +L Sbjct: 1088 YRMLLQL 1094 >ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella thermoacetica ATCC 39073] Length = 368 Score = 134 bits (337), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 93/258 (36%), Positives = 134/258 (51%), Gaps = 24/258 (9%) Query: 444 FLIPAKLAVRYPQEEPWTSKREKLGIYSYNPDKGIWSYIGGVTAAEDGFVQAKISASGLY 503 F +P L +++ P K E L + Y+ K W I V G + A+I+ Y Sbjct: 124 FAVPVTLYLKFAL--PSLVKPENLALAWYDKTKVSWVAIPAVVDVSKGLILARINHFSDY 181 Query: 504 AVMLREAQ--FTDI---SGHWAQQDVEVLAARGIVNGMNGTVFAPKAVLTRAEFTKLLTA 558 AV ++EA+ F D+ S WAQ +E LA GIV G++G+ F P +TRAEF LL Sbjct: 182 AVFVKEARKAFLDVTPASFGWAQDTIETLAGAGIVAGVDGSHFEPGRAVTRAEFASLLVK 241 Query: 559 ALGLQPVHTDKPTFRDVSAAHWSYPYVEXXXXXGIVTG-DAGLFHGNAPLTREQMMVMLM 617 ALGL+ +K F+DV W V G+V G + G F + +TRE++ ML+ Sbjct: 242 ALGLEGKAGEKNPFKDVKGDAWYAGAVTAAASNGLVKGYEDGTFRPDNVITREELAAMLV 301 Query: 618 RAIDSTTAHSPASAADSVKLQAVLSGFKDNGTISQWARESVAAAVDKKLVQG--SGQFLK 675 RA++ KL FKDN +S WAR+SVA A LV+G G F + Sbjct: 302 RAVNLPAGEE--------KL-----AFKDNDKVSTWARKSVAVAAAHGLVKGFEDGTF-R 347 Query: 676 PKDSSTRAEAAVIMYKLL 693 P D+++RAE AV++Y++L Sbjct: 348 PGDAASRAECAVMVYRVL 365 >gb|AAB00841.1| pullulanase [Thermoanaerobacterium thermosulfurigenes] Length = 1861 Score = 134 bits (336), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 79/229 (34%), Positives = 111/229 (48%), Gaps = 10/229 (4%) Query: 466 KLGIYSYNPDKGIWSYIGGVTAAEDGFVQAKISASGLYAVMLREAQFTDISGHWAQQDVE 525 K+ +Y YNP W Y+GG A G + + YA + F DI +WA+ +E Sbjct: 1636 KVAVYYYNPTTNQWEYVGGKVDASSGTITFNATHFSQYAAFEYDKTFNDIKDNWAKDVIE 1695 Query: 526 VLAARGIVNGMNGTVFAPKAVLTRAEFTKLLTAALGLQPVHTDKPTFRDVSAAHWSYPYV 585 VLA+R IV GM T + P +TRAEFT ++ L ++ T F DV + W + Sbjct: 1696 VLASRHIVEGMTDTQYEPNKTVTRAEFTAMILRLLNIKE-ETYSGEFSDVKSGDWYANAI 1754 Query: 586 EXXXXXGIVTGDAGLFHGNAPLTREQMMVMLMRAIDSTTAHSPASAADSVKLQAVLSGFK 645 E GI+ GD N +TRE+M + MRA + T + + + F Sbjct: 1755 EAAYKAGIIEGDGKNARPNDSITREEMTAIAMRAYEMLTQYKEENIG--------ATTFS 1806 Query: 646 DNGTISQWARESVAAAVDKKLVQGS-GQFLKPKDSSTRAEAAVIMYKLL 693 D+ +IS WAR VA A +V G PK ++TRAEAA I+Y LL Sbjct: 1807 DDKSISDWARNVVANAAKLGIVNGEPNNVFAPKGNATRAEAAAIIYGLL 1855 >sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pullulanase) (Pullulanase type II) [Includes: Alpha-amylase (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosidase)] Length = 1861 Score = 134 bits (336), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 79/229 (34%), Positives = 111/229 (48%), Gaps = 10/229 (4%) Query: 466 KLGIYSYNPDKGIWSYIGGVTAAEDGFVQAKISASGLYAVMLREAQFTDISGHWAQQDVE 525 K+ +Y YNP W Y+GG A G + + YA + F DI +WA+ +E Sbjct: 1636 KVAVYYYNPTTNQWEYVGGKVDASSGTITFNATHFSQYAAFEYDKTFNDIKDNWAKDVIE 1695 Query: 526 VLAARGIVNGMNGTVFAPKAVLTRAEFTKLLTAALGLQPVHTDKPTFRDVSAAHWSYPYV 585 VLA+R IV GM T + P +TRAEFT ++ L ++ T F DV + W + Sbjct: 1696 VLASRHIVEGMTDTQYEPNKTVTRAEFTAMILRLLNIKD-ETYSGEFSDVKSGDWYANAI 1754 Query: 586 EXXXXXGIVTGDAGLFHGNAPLTREQMMVMLMRAIDSTTAHSPASAADSVKLQAVLSGFK 645 E GI+ GD N +TRE+M + MRA + T + + + F Sbjct: 1755 EAAYKAGIIEGDGKNARPNDSITREEMTAIAMRAYEMLTQYKEENIG--------ATTFS 1806 Query: 646 DNGTISQWARESVAAAVDKKLVQGS-GQFLKPKDSSTRAEAAVIMYKLL 693 D+ +IS WAR VA A +V G PK ++TRAEAA I+Y LL Sbjct: 1807 DDKSISDWARNVVANAAKLGIVNGEPNNVFAPKGNATRAEAAAIIYGLL 1855 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 927,061,524 Number of extensions: 37679915 Number of successful extensions: 65355 Number of sequences better than 1.0e-05: 138 Number of HSP's gapped: 65038 Number of HSP's successfully gapped: 147 Length of query: 699 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 566 Effective length of database: 396,129,411 Effective search space: 224209246626 Effective search space used: 224209246626 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)