BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0430.0004.p (151 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|O32721|SP22_PAEPO Anti-sigma F factor (Stage II sporulation p... 213 1e-54 pdb|1L0O|A Chain A, Crystal Structure Of The Bacillus Stearother... 172 3e-42 sp|O32727|SP22_BACST Anti-sigma F factor (Stage II sporulation p... 170 1e-41 ref|NP_242403.1| anti-sigma F factor (stage II sporulation prote... 167 9e-41 sp|P26778|SP22_BACLI Anti-sigma F factor (Stage II sporulation p... 164 6e-40 >sp|O32721|SP22_PAEPO Anti-sigma F factor (Stage II sporulation protein AB) gb|AAB81185.1| spoIIAB gene product [Paenibacillus polymyxa] Length = 151 Score = 213 bits (541), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 104/151 (68%), Positives = 116/151 (76%) Query: 1 MTKSEAGNFMNVQFAALSENEXXXXXXXXXXXXXLDPTMEELNDLKTVVSEAVTNCIIHG 60 M + NFM++QFAA SENE LDPTM+EL+DLKTVVSEAVTN IIHG Sbjct: 1 MREGTGNNFMSLQFAAKSENEAFARVAVAAFISRLDPTMDELSDLKTVVSEAVTNSIIHG 60 Query: 61 YDSDPEGIVSISASIEHETVHLTIEDKGRGIEDLELAQQPLYTSKPELERSGMGFTIMEN 120 YDSDP G+V+I IE + + L +ED GRGIEDLELA+QPLYTSKPELERSGMGFTIMEN Sbjct: 61 YDSDPSGVVTIKVGIEADVITLVVEDAGRGIEDLELAKQPLYTSKPELERSGMGFTIMEN 120 Query: 121 FMDEFEVTSEPGRGTSISMKKTIVSKKALYN 151 FMDEFE SEPG GTS+ MKK I SKKALYN Sbjct: 121 FMDEFEAVSEPGGGTSVRMKKRIESKKALYN 151 >pdb|1L0O|A Chain A, Crystal Structure Of The Bacillus Stearothermophilus Anti- Sigma Factor Spoiiab With The Sporulation Sigma Factor Sigmaf pdb|1L0O|B Chain B, Crystal Structure Of The Bacillus Stearothermophilus Anti- Sigma Factor Spoiiab With The Sporulation Sigma Factor Sigmaf Length = 150 Score = 172 bits (435), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 86/140 (61%), Positives = 104/140 (74%) Query: 8 NFMNVQFAALSENEXXXXXXXXXXXXXLDPTMEELNDLKTVVSEAVTNCIIHGYDSDPEG 67 N M++QF+A SENE LDPTM+EL ++KTVVSEAVTN IIHGY++DP G Sbjct: 3 NEMHLQFSARSENESFARVTVAAFVAQLDPTMDELTEIKTVVSEAVTNAIIHGYNNDPNG 62 Query: 68 IVSISASIEHETVHLTIEDKGRGIEDLELAQQPLYTSKPELERSGMGFTIMENFMDEFEV 127 IVSIS IE VHLT+ D+G GI D+E A+QPL+T+KPELERSGMGFTIMENFMDE V Sbjct: 63 IVSISVIIEDGVVHLTVRDEGVGIPDIEEARQPLFTTKPELERSGMGFTIMENFMDEVIV 122 Query: 128 TSEPGRGTSISMKKTIVSKK 147 SE +GT++ +KK IV K Sbjct: 123 ESEVNKGTTVYLKKHIVKSK 142 >sp|O32727|SP22_BACST Anti-sigma F factor (Stage II sporulation protein AB) gb|AAB81193.1| putative [Geobacillus stearothermophilus] Length = 146 Score = 170 bits (430), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 85/144 (59%), Positives = 104/144 (72%) Query: 8 NFMNVQFAALSENEXXXXXXXXXXXXXLDPTMEELNDLKTVVSEAVTNCIIHGYDSDPEG 67 N M++QF+A SENE LDPT +EL ++KTVVSEAVTN IIHGY++DP G Sbjct: 3 NEMHLQFSARSENESFARVTVAAFVAQLDPTTDELTEIKTVVSEAVTNAIIHGYNNDPNG 62 Query: 68 IVSISASIEHETVHLTIEDKGRGIEDLELAQQPLYTSKPELERSGMGFTIMENFMDEFEV 127 IVSIS IE VHLT+ D+G GI D+E A+QPL+T+KPELERSGMGFTIMENFMDE V Sbjct: 63 IVSISVIIEDGVVHLTVRDEGVGIPDIEEARQPLFTTKPELERSGMGFTIMENFMDEVIV 122 Query: 128 TSEPGRGTSISMKKTIVSKKALYN 151 SE +GT++ +KK K+ L N Sbjct: 123 ESEVNKGTTVYLKKAYCEKQTLCN 146 >ref|NP_242403.1| anti-sigma F factor (stage II sporulation protein AB) [Bacillus halodurans] sp|Q9KCN2|SP22_BACHD Anti-sigma F factor (Stage II sporulation protein AB) pir||A83842 anti-sigma F factor (stage II sporulation protein AB) spoIIAB [imported] - Bacillus halodurans (strain C-125) dbj|BAB05256.1| anti-sigma F factor (stage II sporulation protein AB) [Bacillus halodurans] Length = 146 Score = 167 bits (422), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 82/144 (56%), Positives = 102/144 (70%) Query: 8 NFMNVQFAALSENEXXXXXXXXXXXXXLDPTMEELNDLKTVVSEAVTNCIIHGYDSDPEG 67 N MN+ F+A S+NE LDPTM+E+ ++KTVVSEAVTN IIHGY P+G Sbjct: 3 NEMNLSFSAQSQNESFARVTVGAFIAQLDPTMDEMTEIKTVVSEAVTNAIIHGYQGQPDG 62 Query: 68 IVSISASIEHETVHLTIEDKGRGIEDLELAQQPLYTSKPELERSGMGFTIMENFMDEFEV 127 +V I ASI+ LTI D+G GI D+E A+QPLYT+KPELERSGMGFTIMENFMDE +V Sbjct: 63 VVYIHASIDQGVFELTIRDEGMGISDVEEARQPLYTTKPELERSGMGFTIMENFMDEVKV 122 Query: 128 TSEPGRGTSISMKKTIVSKKALYN 151 SEP GT++ +KK + KA+ N Sbjct: 123 VSEPMIGTTVFLKKHLTKSKAICN 146 >sp|P26778|SP22_BACLI Anti-sigma F factor (Stage II sporulation protein AB) pir||B37165 sporulation locus spoIIA protein B - Bacillus licheniformis gb|AAA22796.1| sporulation protein Length = 146 Score = 164 bits (415), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 82/144 (56%), Positives = 102/144 (70%) Query: 8 NFMNVQFAALSENEXXXXXXXXXXXXXLDPTMEELNDLKTVVSEAVTNCIIHGYDSDPEG 67 N MN+QF ALS+NE LDPTM+EL ++KTVVSEAVTN IIHGY++ +G Sbjct: 3 NEMNIQFTALSQNESFARVTVAAFIAQLDPTMDELTEIKTVVSEAVTNAIIHGYENSGQG 62 Query: 68 IVSISASIEHETVHLTIEDKGRGIEDLELAQQPLYTSKPELERSGMGFTIMENFMDEFEV 127 V IS ++E V+LTI D+G GI DLE A+QPL+T+KPELERSGMGFTIMENFMD+ + Sbjct: 63 NVYISVTLEDHIVYLTIRDEGVGIPDLEEARQPLFTTKPELERSGMGFTIMENFMDDISI 122 Query: 128 TSEPGRGTSISMKKTIVSKKALYN 151 S P GT+I + K + KAL N Sbjct: 123 DSSPEMGTTIHLTKHLSKSKALCN 146 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.310 0.128 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 189,939,239 Number of extensions: 6718474 Number of successful extensions: 14879 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 14980 Number of HSP's successfully gapped: 40 Length of query: 151 Length of database: 757,237,578 Length adjustment: 110 Effective length of query: 41 Effective length of database: 458,576,688 Effective search space: 18801644208 Effective search space used: 18801644208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 120 (50.8 bits)