BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0007.p (335 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00059726.1| COG2152: Predicted glycosylase [Clostridium t... 164 4e-39 ref|NP_229030.1| conserved hypothetical protein [Thermotoga mari... 162 7e-39 ref|NP_406008.1| conserved hypothetical protein [Yersinia pestis... 154 2e-36 ref|ZP_00077716.1| COG2152: Predicted glycosylase [Methanosarcin... 149 8e-35 ref|ZP_00076614.1| COG2152: Predicted glycosylase [Methanosarcin... 147 4e-34 >ref|ZP_00059726.1| COG2152: Predicted glycosylase [Clostridium thermocellum ATCC 27405] Length = 334 Score = 164 bits (414), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 113/331 (34%), Positives = 174/331 (52%), Gaps = 36/331 (10%) Query: 17 IRRHPANPVLDAARVPYPTALVFNAGVAKFGGRYVMVFRNDYGSLEKQTIEPSHTTDLGI 76 +R P NP+L A PY VFN + G+ +++ R + +++ SH T Sbjct: 25 FKRFPGNPILSAKNWPYAANTVFNPAATMYNGKVLLLIRVE----DRRGF--SHLTK--- 75 Query: 77 AFSDDGI-HWTAGPKPVFKLRDEEIVR----AYDPRLTVID--GRCYMCFAVDTRHGIRG 129 A SDDGI +W KP + E+ DPR+T I+ G+ + + ++ G Sbjct: 76 AISDDGISNWIIDDKPTLEAEPEKFPEEEWGVEDPRITWIEELGKFAVVYTAYSKGGPLV 135 Query: 130 GIAVTDDFESFEIL-SLSTPDLRNMVLFPERIGGNFVRLERPFTVYSRGGVDRFDTWISE 188 +A+T+DFE+FE L ++ P+ ++ LFP RI G +V + RP +++ G WIS Sbjct: 136 SLALTEDFENFEKLGAIMPPEDKDAALFPRRINGKWVLIHRPISIHHGPGAH---IWISR 192 Query: 189 SPDLIYWGRSELLLSVEQVPFAN-DKIGPAAPPVKTSKGWLTTFHAVDIDPARGKNGWEP 247 S DL YWG ++L+ + + + +K+G PP++T GWL +H V A G Sbjct: 193 SDDLKYWGDHQILIRARKGGWWDANKVGLNCPPLETPDGWLILYHGVR-QTASGS----- 246 Query: 248 SWKKRYTAGIMLLDLDNPKKILGMSASPLLAPEAAYETDGGFRNNVIFPGGMVL-EEDGE 306 Y G+ LLDL+NP K+L S + P+ YE +G ++V+FP G V E+ GE Sbjct: 247 ----IYRLGLALLDLENPSKVLRRSDEWVFGPQEFYEREGDV-DDVVFPCGWVYNEKTGE 301 Query: 307 VKIYYGAADTVECLATAHVDDL---IRLCLE 334 +KIYYGAADT +AT ++ DL I+ C E Sbjct: 302 IKIYYGAADTCIAMATTNISDLLDYIKRCPE 332 >ref|NP_229030.1| conserved hypothetical protein [Thermotoga maritima] pir||B72278 conserved hypothetical protein - Thermotoga maritima (strain MSB8) gb|AAD36300.1| conserved hypothetical protein [Thermotoga maritima] Length = 326 Score = 162 bits (411), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 120/322 (37%), Positives = 167/322 (51%), Gaps = 34/322 (10%) Query: 16 PIRRHPANPVLDAARVPYPTALVFNAGVAKFGGRYVMVFRNDYGSLEKQTIEPSHTTDLG 75 P+ R+ NP++ VP A VFN+ V + G +V VFR D+ + L Sbjct: 23 PVWRYSKNPIIGRNPVP-KGARVFNSAVVPYNGEFVGVFRIDHKNTRPF---------LH 72 Query: 76 IAFSDDGIHWTAGPKPV--FKLRDE--EIVRAYDPRLTVIDGRCYMCFAVDTRHGIRGGI 131 S DGI+W P+ + + E + AYDPR+ I+ Y+ F D HG G+ Sbjct: 73 FGRSKDGINWEIEPEEIQWVDVNGEPFQPSYAYDPRVVKIEDTYYITFCTDD-HGPTIGV 131 Query: 132 AVTDDFESFEIL-SLSTPDLRNMVLFPERIGGNFVRLERPFTVYSRGGVDRF-DTWISES 189 +T DF++F L + P RN VLFP +I G +V L RP S G F D ++SES Sbjct: 132 GMTKDFKTFVRLPNAYVPFNRNGVLFPRKINGKYVMLNRP----SDNGHTPFGDIFLSES 187 Query: 190 PDLIYWGRSELLLSVEQVPF-ANDKIGPAAPPVKTSKGWLTTFHAVDIDPARGKNGWEPS 248 PD+I+WG +L + N KIG P++TS+GWL +H V + NG+ Sbjct: 188 PDMIHWGNHRFVLGRSSYNWWENLKIGAGPYPIETSEGWLLIYHGVTLT----CNGY--- 240 Query: 249 WKKRYTAGIMLLDLDNPKKILGMSASPLLAPEAAYETDGGFRNNVIFPGGMVLEED-GEV 307 Y+ G LLDLD+P K+L S LL PE YET G F NV+FP + + D G V Sbjct: 241 ---VYSFGAALLDLDDPSKVLYRSRYYLLTPEEEYETVG-FVPNVVFPCAALCDADTGRV 296 Query: 308 KIYYGAADTVECLATAHVDDLI 329 IYYGAADT LA ++D+++ Sbjct: 297 AIYYGAADTHVALAFGYIDEIV 318 >ref|NP_406008.1| conserved hypothetical protein [Yersinia pestis] ref|NP_669032.1| hypothetical [Yersinia pestis KIM] ref|NP_993622.1| Predicted glycosylase [Yersinia pestis biovar Mediaevails str. 91001] pir||AC0302 conserved hypothetical protein YPO2474 [imported] - Yersinia pestis (strain CO92) emb|CAC91279.1| conserved hypothetical protein [Yersinia pestis CO92] gb|AAM85283.1| hypothetical [Yersinia pestis KIM] gb|AAS62499.1| Predicted glycosylase [Yersinia pestis biovar Mediaevails str. 91001] Length = 351 Score = 154 bits (390), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 95/259 (36%), Positives = 137/259 (52%), Gaps = 21/259 (8%) Query: 65 TIEPSHTTDLGIAFSDDGIHWTAGPKP-VFKLRDEEIVRAYDPRLTVIDGRCYMCFAVDT 123 T+ + + L +A S DG+ + +P +F +E D R+T IDG Y+ ++ + Sbjct: 99 TVYLTSLSHLRVARSHDGVTFVVDDQPFIFPANGDEAFGCEDARITQIDGAYYINYSAVS 158 Query: 124 RHGIRGGIAVTDDFESFEILSLS-TPDLRNMVLFPERIGGNFVRLERPFTVYSRGGVDRF 182 GI +AVT DF + L L PD R+ LFPE+I G ++ L RP + + Sbjct: 159 AKGICTALAVTQDFTDVKRLGLIFCPDNRDACLFPEKINGKYMTLHRP----APKHFGKP 214 Query: 183 DTWISESPDLIYWGRSELLLSVEQVPFANDKIGPAAPPVKTSKGWLTTFHAVDIDPARGK 242 + W++ S DL++WG LL Q P+ K+G A +KT KGWL +H VD Sbjct: 215 EIWLASSLDLLHWGDHRHLLGTSQDPWDALKLGGGAQMLKTEKGWLQIYHGVDA------ 268 Query: 243 NGWEPSWKKRYTAGIMLLDLDNPKKILGMSASPLLAPEAAYETDGGFRNNVIFPGGMVLE 302 +RY+ G +LLDL+ P KIL S PLL P+A YE GF NV+F G ++E Sbjct: 269 -------TQRYSLGALLLDLEQPTKILAKSPVPLLQPQAPYELH-GFFGNVVFTCGALIE 320 Query: 303 EDGEVKIYYGAADTVECLA 321 D +++YYGAAD CLA Sbjct: 321 ND-SLRVYYGAADECMCLA 338 >ref|ZP_00077716.1| COG2152: Predicted glycosylase [Methanosarcina barkeri] Length = 344 Score = 149 bits (377), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 111/326 (34%), Positives = 162/326 (49%), Gaps = 35/326 (10%) Query: 19 RHPANPVLDAARVPYPTALVFNAGVAKFGGRYVMVFR-NDYGSLEKQTIEPSHTTDLGIA 77 R+ NP+L PY VFN A G +++ R D+ SH T IA Sbjct: 12 RYDRNPILTVDEWPYRAHSVFNPAAAIVDGTTLLLVRVEDHRGF-------SHFT---IA 61 Query: 78 FSDDGIH-WTAGPKPVFKL----RDEEIVRAYDPRLTVIDG--RCYMCFAVDTRHGIRGG 130 S +GI+ W P+P F EEI DPR+T ID + + + + G Sbjct: 62 RSKNGIYGWEIDPEPTFAPDPVGYPEEIYGIEDPRITYIDEMEKWAVAYTAFSDSGPLTA 121 Query: 131 IAVTDDFESFEILSLSTP-DLRNMVLFPERIGGNFVRLERPFTVYSRGGVDRFDTWISES 189 +A TDDF +F+ + + P + ++ +FP R G + L RP ++ S + + WIS S Sbjct: 122 LAFTDDFRNFDRIGATLPPENKDAAVFPVRFNGRWAMLHRPVSLISGA---KANIWISFS 178 Query: 190 PDLIYWGRSELLLSVEQVPFAN-DKIGPAAPPVKTSKGWLTTFHAVDIDPARGKNGWEPS 248 PD+ YWG E+LL + + + +KIG + P++ S GWL +H V A Sbjct: 179 PDMKYWGEHEVLLYAREGGWWDANKIGLSPQPIRVSDGWLIMYHGVRQTTA--------- 229 Query: 249 WKKRYTAGIMLLDLDNPKKILGMSASPLLAPEAAYETDGGFRNNVIFPGGMVLEEDGEVK 308 K Y G+ LLD D+P+K+L S + P YE G N+V+FP G VL D E++ Sbjct: 230 -KASYRLGLALLDPDDPRKVLHRSEGWVFGPREMYERSGDV-NDVVFPCGWVLAGD-EIR 286 Query: 309 IYYGAADTVECLATAHVDDLIRLCLE 334 IYYG+ADT LATA V D+++ E Sbjct: 287 IYYGSADTSVSLATAKVRDVLKYIHE 312 >ref|ZP_00076614.1| COG2152: Predicted glycosylase [Methanosarcina barkeri] Length = 324 Score = 147 bits (371), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 101/321 (31%), Positives = 162/321 (50%), Gaps = 33/321 (10%) Query: 19 RHPANPVLDAARVPYPTALVFNAGVAKFGGRYVMVFRNDYGSLEKQTIEPSHTTDLGIAF 78 R+ NP+L A PY + VFN +++ R + + + L +A Sbjct: 16 RYKKNPILTADYWPYKVSSVFNPAAVMVDNTTILLVRAE---------DHRGFSHLTVAR 66 Query: 79 SDDGIH-WTAGPKPVFK----LRDEEIVRAYDPRLTVID--GRCYMCFAVDTRHGIRGGI 131 S +GI W PKP F EE+ DPR+T ID G+ + + + G + Sbjct: 67 SKNGIDGWEIDPKPTFTSDPTYYSEEVYGIEDPRITYIDEMGKWAIAYTAFSDSGPLTSL 126 Query: 132 AVTDDFESFE-ILSLSTPDLRNMVLFPERIGGNFVRLERPFTVYSRGGVDRFDTWISESP 190 A T++F +FE + + P+ ++ +FP R+ G + L RP + + + + WIS SP Sbjct: 127 AYTENFTNFERVGPIMPPENKDAAIFPTRLNGRWAMLHRPVSNIAGA---KANLWISFSP 183 Query: 191 DLIYWGRSELLLSVEQVPFAN-DKIGPAAPPVKTSKGWLTTFHAVDIDPARGKNGWEPSW 249 D+ YWG ++LL + + + KIG A PP+KTS GWL ++ V + + Sbjct: 184 DMKYWGEHKVLLYAREGGWWDARKIGLAPPPMKTSDGWLILYYGVR----------QTTS 233 Query: 250 KKRYTAGIMLLDLDNPKKILGMSASPLLAPEAAYETDGGFRNNVIFPGGMVLEEDGEVKI 309 KK Y G+ LLDL++P K+L S + P YE G N+V+FP G +L D E++I Sbjct: 234 KKSYRLGLALLDLEDPTKVLHRSEGWVFGPREMYERTGDV-NDVVFPCGWILAGD-ELRI 291 Query: 310 YYGAADTVECLATAHVDDLIR 330 YYG+AD +A+A + D+++ Sbjct: 292 YYGSADMSVAMASAKMSDVMQ 312 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.140 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 556,901,590 Number of extensions: 24643846 Number of successful extensions: 41324 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 41192 Number of HSP's successfully gapped: 32 Length of query: 335 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 209 Effective length of database: 415,135,104 Effective search space: 86763236736 Effective search space used: 86763236736 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)