BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0016.p (432 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00099050.1| COG1067: Predicted ATP-dependent protease [De... 561 e-158 ref|NP_243917.1| ATP-dependent proteinase La [Bacillus haloduran... 540 e-152 ref|NP_834190.1| ATP-dependent protease La [Bacillus cereus ATCC... 538 e-152 ref|NP_658502.1| AAA, ATPase family associated with various cell... 536 e-151 ref|NP_846916.1| ATP-dependent protease LA [Bacillus anthracis s... 536 e-151 >ref|ZP_00099050.1| COG1067: Predicted ATP-dependent protease [Desulfitobacterium hafniense] Length = 579 Score = 561 bits (1445), Expect = e-158, Method: Compositional matrix adjust. Identities = 266/392 (67%), Positives = 322/392 (82%) Query: 3 LSILLMIVQLFFALVIGVYFWNLLRGQKTNKTAVDRESRKELDKLRKMRMISLTKPLSEK 62 L+ ++M+VQL FA++IG+YFW LL+ Q+ NK +++RES+KEL+KL KMR I+LT PL+EK Sbjct: 4 LTGIVMMVQLVFAVIIGLYFWGLLKSQQGNKVSIERESKKELEKLEKMRKIALTTPLAEK 63 Query: 63 TRPASIGDIVGQKDGLRALKAALCSANPQHVIIYGPPGVGKTAAARVVMEEAKKNGLSPF 122 TRP +IVGQ +G++AL+AALC NPQHV+IYGPPGVGKTAAAR+V+EEA+KN +SPF Sbjct: 64 TRPTHFEEIVGQLEGIKALRAALCGPNPQHVLIYGPPGVGKTAAARLVLEEARKNPMSPF 123 Query: 123 KSDAKFTEIDATTARFDERGIADPLIGSVHDPIYQGAGAMGVAGVPQPKPGAVTKAHGGI 182 K DAKF EID TARFDERGIADPLIGSVHDPIYQGAGAMG+AG+PQPK GAVTKAHGG+ Sbjct: 124 KEDAKFIEIDGATARFDERGIADPLIGSVHDPIYQGAGAMGMAGIPQPKAGAVTKAHGGV 183 Query: 183 LFLDEIGELHPIQMNKLLKVLEDRKVLLESAYYNSEDSNTPAYIHDIFQNGLPADFRLVG 242 LF+DEIGELHPIQ+NKLLKVLEDRKV+LESAYY+SED+N P +IHDIFQNGLPADFR+VG Sbjct: 184 LFIDEIGELHPIQINKLLKVLEDRKVILESAYYSSEDTNIPVHIHDIFQNGLPADFRMVG 243 Query: 243 ATTRSPEEISPALRSRCMEIYFRPLLPEEIAVIGRDAVQKIGLKPSPEAIEVVQQYATNG 302 ATTRSPEEI PA+RSRCME++FR LLPEEIA I R A K+ +P A+ V +QYATNG Sbjct: 244 ATTRSPEEIPPAIRSRCMEVFFRSLLPEEIAQIARGAAAKMETPITPSALNVAKQYATNG 303 Query: 303 REAVNMIQLAAGLALTEGRDTLXXXXXXXXXXXXQLPMRTERKIPSSPQIGLVNGLAVYG 362 REAVN++QLAAG+A TE + + Q R ERK+P ++GLVNGLAVYG Sbjct: 304 REAVNIVQLAAGVAQTEKKLEINAATVEWVVTSGQYAPRPERKVPLVAEVGLVNGLAVYG 363 Query: 363 PGMGTLLEIEVSAAPAKSGQGRLNVTGVVDEE 394 P MG L+E+EV+A P G G L VTG+V+EE Sbjct: 364 PNMGMLIELEVTAHPVAPGTGELLVTGIVEEE 395 >ref|NP_243917.1| ATP-dependent proteinase La [Bacillus halodurans] pir||C84031 ATP-dependent proteinase La lonB [imported] - Bacillus halodurans (strain C-125) dbj|BAB06770.1| ATP-dependent proteinase La [Bacillus halodurans] Length = 556 Score = 540 bits (1390), Expect = e-152, Method: Compositional matrix adjust. Identities = 256/394 (64%), Positives = 311/394 (78%), Gaps = 1/394 (0%) Query: 1 MELSILLMIVQLFFALVIGVYFWNLLRGQKTNKTAVDRESRKELDKLRKMRMISLTKPLS 60 M + +L +VQLFF +VIG+YFWNLL+ Q++ + ++DRESRKE+D+LRK++ ISL++PLS Sbjct: 1 MNWTGILFVVQLFFGVVIGLYFWNLLKTQRSQRVSIDRESRKEMDQLRKLQSISLSEPLS 60 Query: 61 EKTRPASIGDIVGQKDGLRALKAALCSANPQHVIIYGPPGVGKTAAARVVMEEAKKNGLS 120 EK RP S DIVGQK+G+RALKAALC NPQHVIIYGPPGVGKTAAAR+V++EAKKN S Sbjct: 61 EKVRPESFEDIVGQKEGIRALKAALCGPNPQHVIIYGPPGVGKTAAARLVLQEAKKNPNS 120 Query: 121 PFKSDAKFTEIDATTARFDERGIADPLIGSVHDPIYQGAGAMGVAGVPQPKPGAVTKAHG 180 PF DA F E+D TTARFDERGIADPLIGSVHDPIYQGAGAMG AG+PQPK GAVTKAHG Sbjct: 121 PFAHDAVFVELDGTTARFDERGIADPLIGSVHDPIYQGAGAMGQAGIPQPKQGAVTKAHG 180 Query: 181 GILFLDEIGELHPIQMNKLLKVLEDRKVLLESAYYNSEDSNTPAYIHDIFQNGLPADFRL 240 G+LF+DEIGELH IQ+NKLLKVLEDRKV LESAYY+ E+ P +IH+IFQ GLPADFRL Sbjct: 181 GMLFIDEIGELHSIQINKLLKVLEDRKVFLESAYYSEENQQIPRHIHEIFQRGLPADFRL 240 Query: 241 VGATTRSPEEISPALRSRCMEIYFRPLLPEEIAVIGRDAVQKIGLKPSPEAIEVVQQYAT 300 VGATTR PEEI PA+RSRC+E+YFR L EEI I + AV ++G K E + YAT Sbjct: 241 VGATTRMPEEIPPAIRSRCLEVYFRSLKREEIVTIAKKAVNRLGFKAEEGVYEKISAYAT 300 Query: 301 NGREAVNMIQLAAGLALTEGRDTLXXXXXXXXXXXXQLPMRTERKIPSSPQIGLVNGLAV 360 NGRE VN++Q+AAG+A TEGR + QL R ER+IPS P++GLVNGLAV Sbjct: 301 NGRETVNIVQIAAGMATTEGRHEVTVADVEWVIHSSQLSPRPERQIPSEPKVGLVNGLAV 360 Query: 361 YGPGMGTLLEIEVSAAPAKSGQGRLNVTGVVDEE 394 +GP G LLE+EV P+ + +G + +TG+ +EE Sbjct: 361 FGPNTGALLEVEVDVIPSVA-EGSVTITGIAEEE 393 >ref|NP_834190.1| ATP-dependent protease La [Bacillus cereus ATCC 14579] gb|AAP11391.1| ATP-dependent protease La [Bacillus cereus ATCC 14579] Length = 556 Score = 538 bits (1386), Expect = e-152, Method: Compositional matrix adjust. Identities = 253/394 (64%), Positives = 318/394 (80%), Gaps = 1/394 (0%) Query: 1 MELSILLMIVQLFFALVIGVYFWNLLRGQKTNKTAVDRESRKELDKLRKMRMISLTKPLS 60 M + + ++VQL F +++G+YFW+LLR Q+T K ++DRES+KEL++LRKMR ISLT+PL+ Sbjct: 1 MSWTNIFLVVQLVFGVIVGLYFWHLLRNQRTQKVSIDRESKKELEQLRKMREISLTEPLA 60 Query: 61 EKTRPASIGDIVGQKDGLRALKAALCSANPQHVIIYGPPGVGKTAAARVVMEEAKKNGLS 120 EK RP + DIVGQ+DG+++LKAALC NPQHVIIYGPPGVGKTAAAR+V+EEAK+N S Sbjct: 61 EKVRPTTFLDIVGQEDGIKSLKAALCGPNPQHVIIYGPPGVGKTAAARLVLEEAKRNPKS 120 Query: 121 PFKSDAKFTEIDATTARFDERGIADPLIGSVHDPIYQGAGAMGVAGVPQPKPGAVTKAHG 180 PF+++A F E+DATTARFDERGIADPLIGSVHDPIYQGAGAMG AG+PQPK GAVT AHG Sbjct: 121 PFRTNATFIELDATTARFDERGIADPLIGSVHDPIYQGAGAMGQAGIPQPKKGAVTDAHG 180 Query: 181 GILFLDEIGELHPIQMNKLLKVLEDRKVLLESAYYNSEDSNTPAYIHDIFQNGLPADFRL 240 GILF+DEIGELHPIQMNK+LKVLEDRKV LESAYY+ E++ P YIHDIFQ GLPADFRL Sbjct: 181 GILFIDEIGELHPIQMNKMLKVLEDRKVFLESAYYSEENTMIPTYIHDIFQKGLPADFRL 240 Query: 241 VGATTRSPEEISPALRSRCMEIYFRPLLPEEIAVIGRDAVQKIGLKPSPEAIEVVQQYAT 300 VGATTRSPEEI PA+RSRC+E++FR L EEI + ++A +K+ ++ IE++ YA Sbjct: 241 VGATTRSPEEIPPAIRSRCLEVFFRELDTEEIQKVAKNAAEKVEMQIGENGIEMIGMYAR 300 Query: 301 NGREAVNMIQLAAGLALTEGRDTLXXXXXXXXXXXXQLPMRTERKIPSSPQIGLVNGLAV 360 NGREA+N++Q++AG+A+ E R + QL + E++I P+IGLVNGLAV Sbjct: 301 NGREAINLVQISAGMAINEERSFIKDEDIEWVVHSSQLTPKYEKRIYPIPRIGLVNGLAV 360 Query: 361 YGPGMGTLLEIEVSAAPAKSGQGRLNVTGVVDEE 394 YGP G LLEIEV+A AK +G +NVTG+V+EE Sbjct: 361 YGPNTGALLEIEVTAIKAKD-KGSVNVTGIVEEE 393 >ref|NP_658502.1| AAA, ATPase family associated with various cellular activities (AAA) [Bacillus anthracis A2012] Length = 556 Score = 536 bits (1382), Expect = e-151, Method: Compositional matrix adjust. Identities = 254/394 (64%), Positives = 316/394 (80%), Gaps = 1/394 (0%) Query: 1 MELSILLMIVQLFFALVIGVYFWNLLRGQKTNKTAVDRESRKELDKLRKMRMISLTKPLS 60 M + + ++VQL F +++G+YFW+LLR Q+T K ++DRES+KEL++LRKMR ISLT+PL+ Sbjct: 1 MSWTNIFLLVQLVFGVIVGLYFWHLLRNQRTQKVSIDRESKKELEQLRKMREISLTEPLA 60 Query: 61 EKTRPASIGDIVGQKDGLRALKAALCSANPQHVIIYGPPGVGKTAAARVVMEEAKKNGLS 120 EK RP S DIVGQ+DG+++LKAALC NPQHVIIYGPPGVGKTAAAR+V+EEAK+N S Sbjct: 61 EKVRPTSFLDIVGQEDGIKSLKAALCGPNPQHVIIYGPPGVGKTAAARLVLEEAKRNPKS 120 Query: 121 PFKSDAKFTEIDATTARFDERGIADPLIGSVHDPIYQGAGAMGVAGVPQPKPGAVTKAHG 180 PF+++A F E+DATTARFDERGIADPLIGSVHDPIYQGAGAMG AG+PQPK GAVT AHG Sbjct: 121 PFRTNATFIELDATTARFDERGIADPLIGSVHDPIYQGAGAMGQAGIPQPKKGAVTDAHG 180 Query: 181 GILFLDEIGELHPIQMNKLLKVLEDRKVLLESAYYNSEDSNTPAYIHDIFQNGLPADFRL 240 GILF+DEIGELHPIQMNK+LKVLEDRKV LESAYY+ E++ P YIHDIFQ GLPADFRL Sbjct: 181 GILFIDEIGELHPIQMNKMLKVLEDRKVFLESAYYSEENTMIPTYIHDIFQKGLPADFRL 240 Query: 241 VGATTRSPEEISPALRSRCMEIYFRPLLPEEIAVIGRDAVQKIGLKPSPEAIEVVQQYAT 300 VGATTRSPEEI PA+RSRC+E++FR L EEI + ++A K+ ++ IE++ YA Sbjct: 241 VGATTRSPEEIPPAIRSRCLEVFFRELDTEEIQKVAKNAADKVEMQIGENGIEMIGMYAR 300 Query: 301 NGREAVNMIQLAAGLALTEGRDTLXXXXXXXXXXXXQLPMRTERKIPSSPQIGLVNGLAV 360 NGREA+N++Q++AG+A+ E R + QL + E+ I P+IGLVNGLAV Sbjct: 301 NGREAINLVQISAGMAINEERSFIKDEDIEWVVHSSQLTPKYEKHIYPIPRIGLVNGLAV 360 Query: 361 YGPGMGTLLEIEVSAAPAKSGQGRLNVTGVVDEE 394 YGP G LLEIEV+A AK +G +NVTG+V+EE Sbjct: 361 YGPNTGALLEIEVTAIKAKD-KGSVNVTGIVEEE 393 >ref|NP_846916.1| ATP-dependent protease LA [Bacillus anthracis str. Ames] gb|AAP28402.1| ATP-dependent protease LA [Bacillus anthracis str. Ames] Length = 557 Score = 536 bits (1382), Expect = e-151, Method: Compositional matrix adjust. Identities = 254/394 (64%), Positives = 316/394 (80%), Gaps = 1/394 (0%) Query: 1 MELSILLMIVQLFFALVIGVYFWNLLRGQKTNKTAVDRESRKELDKLRKMRMISLTKPLS 60 M + + ++VQL F +++G+YFW+LLR Q+T K ++DRES+KEL++LRKMR ISLT+PL+ Sbjct: 2 MSWTNIFLLVQLVFGVIVGLYFWHLLRNQRTQKVSIDRESKKELEQLRKMREISLTEPLA 61 Query: 61 EKTRPASIGDIVGQKDGLRALKAALCSANPQHVIIYGPPGVGKTAAARVVMEEAKKNGLS 120 EK RP S DIVGQ+DG+++LKAALC NPQHVIIYGPPGVGKTAAAR+V+EEAK+N S Sbjct: 62 EKVRPTSFLDIVGQEDGIKSLKAALCGPNPQHVIIYGPPGVGKTAAARLVLEEAKRNPKS 121 Query: 121 PFKSDAKFTEIDATTARFDERGIADPLIGSVHDPIYQGAGAMGVAGVPQPKPGAVTKAHG 180 PF+++A F E+DATTARFDERGIADPLIGSVHDPIYQGAGAMG AG+PQPK GAVT AHG Sbjct: 122 PFRTNATFIELDATTARFDERGIADPLIGSVHDPIYQGAGAMGQAGIPQPKKGAVTDAHG 181 Query: 181 GILFLDEIGELHPIQMNKLLKVLEDRKVLLESAYYNSEDSNTPAYIHDIFQNGLPADFRL 240 GILF+DEIGELHPIQMNK+LKVLEDRKV LESAYY+ E++ P YIHDIFQ GLPADFRL Sbjct: 182 GILFIDEIGELHPIQMNKMLKVLEDRKVFLESAYYSEENTMIPTYIHDIFQKGLPADFRL 241 Query: 241 VGATTRSPEEISPALRSRCMEIYFRPLLPEEIAVIGRDAVQKIGLKPSPEAIEVVQQYAT 300 VGATTRSPEEI PA+RSRC+E++FR L EEI + ++A K+ ++ IE++ YA Sbjct: 242 VGATTRSPEEIPPAIRSRCLEVFFRELDTEEIQKVAKNAADKVEMQIGENGIEMIGMYAR 301 Query: 301 NGREAVNMIQLAAGLALTEGRDTLXXXXXXXXXXXXQLPMRTERKIPSSPQIGLVNGLAV 360 NGREA+N++Q++AG+A+ E R + QL + E+ I P+IGLVNGLAV Sbjct: 302 NGREAINLVQISAGMAINEERSFIKDEDIEWVVHSSQLTPKYEKHIYPIPRIGLVNGLAV 361 Query: 361 YGPGMGTLLEIEVSAAPAKSGQGRLNVTGVVDEE 394 YGP G LLEIEV+A AK +G +NVTG+V+EE Sbjct: 362 YGPNTGALLEIEVTAIKAKD-KGSVNVTGIVEEE 394 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 622,562,917 Number of extensions: 25806894 Number of successful extensions: 93875 Number of sequences better than 1.0e-05: 174 Number of HSP's gapped: 94321 Number of HSP's successfully gapped: 174 Length of query: 432 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 303 Effective length of database: 406,989,807 Effective search space: 123317911521 Effective search space used: 123317911521 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)