BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0008.p (667 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO88279.1| endo-beta-N-acetylglucosaminidase [Bacillus circu... 130 8e-29 ref|ZP_00060097.1| hypothetical protein [Clostridium thermocellu... 127 8e-28 dbj|BAC45001.1| xylanase 5 [Paenibacillus sp. W-61] 125 4e-27 ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella th... 123 1e-26 gb|AAD09354.1| s-layer associated multidomain endoglucanase [The... 117 7e-25 >gb|AAO88279.1| endo-beta-N-acetylglucosaminidase [Bacillus circulans] Length = 1936 Score = 130 bits (328), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 102/276 (36%), Positives = 146/276 (52%), Gaps = 21/276 (7%) Query: 400 SLTSLKLASKVYSFDLTVGGV-----KTSTFKQPLTIKLPLANTTGLDKELLSVSKVVYK 454 S +++ LA VY F L+ G + S F QP+TI L + +G++ E + + + Sbjct: 1669 SPSAITLAGGVYDFKLSAAGANGSYAELSEFDQPITISLKIE--SGVNPEQVGIYYISGN 1726 Query: 455 -TLEFHGG-VVDGDYIVEPRDTFSTYAVVENKVNFNDVASVQAWAGRQIQVVAAKGAIEG 512 L++ GG DG+ E FS YAV++ F+DV + WA I + ++ ++G Sbjct: 1727 GKLDYIGGEYRDGELAAE-VTHFSQYAVLKVVKVFDDVPAGH-WAEGVISKLTSRLMVDG 1784 Query: 513 VGASKFAPKSNVTRAEFSKMLIRALNLENSTATESFGDVASTAWYAPYVAVAAEKGIVTG 572 + F P+ VTRAEF+ +L RAL L + T +F DV + WYA V A E GI G Sbjct: 1785 TSETTFEPERVVTRAEFTALLARALKL-TAGGTPTFADVKAGDWYADAVTAAVEAGIAEG 1843 Query: 573 RSASQFDPNATITRAEMATMIARA---VKTVNPQATAGDASSLTKFADAAKIAASLKDGV 629 +SA QF+P A ITR EM M RA K P +T +AS F D +I+A + V Sbjct: 1844 KSAGQFEPQARITREEMVVMTMRAYNKAKDKGP-STGVEAS----FTDENQISAWAVEQV 1898 Query: 630 AFAASHNLVIGNA-GKFNPNNTATRAEAAVIIYRTI 664 AA+ L+ G A GKF P TATRAEA +I+ + Sbjct: 1899 KAAAALQLIQGRAQGKFEPQGTATRAEAVQVIFNML 1934 >ref|ZP_00060097.1| hypothetical protein [Clostridium thermocellum ATCC 27405] pir||JC6031 scaffoldin dockerin binding protein precursor - Clostridium thermocellum gb|AAB07763.1| SdbA Length = 631 Score = 127 bits (320), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 69/188 (36%), Positives = 109/188 (57%), Gaps = 2/188 (1%) Query: 475 FSTYAVVENKVNFNDVASVQAWAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLI 534 S YAV F D+ S AWA +QI+V+A+KG I G + F P++++TRA+F +L+ Sbjct: 442 LSKYAVSYVYKTFADIGSY-AWAKKQIEVLASKGVINGTSDTTFTPQADITRADFMILLV 500 Query: 535 RALNLENSTATESFGDVASTAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIA 594 +AL L + T +F DV+ +Y YV +A E GI TG ++F+P A ITR +M + Sbjct: 501 KALGL-TAEVTSNFDDVSEKDYYYEYVGIAKELGITTGVGNNKFNPKAKITRQDMMVLTT 559 Query: 595 RAVKTVNPQATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIGNAGKFNPNNTATRA 654 A++ ++ G + + +F+D +IA+ +GVA +V+G+ NP A+RA Sbjct: 560 NALRIAGKISSTGTRADVERFSDKDQIASYAVEGVATLVKEGIVVGSGDIINPRGNASRA 619 Query: 655 EAAVIIYR 662 E A IIY+ Sbjct: 620 ELAAIIYK 627 >dbj|BAC45001.1| xylanase 5 [Paenibacillus sp. W-61] Length = 1326 Score = 125 bits (313), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 87/249 (34%), Positives = 133/249 (53%), Gaps = 16/249 (6%) Query: 428 PLTIKLPLANTTGLDKELLSVSKVVYKTLEFHGGVV---DGDY------IVEPRDTFSTY 478 P+T+ +P T +EL++ ++V ++ G + +G Y V FSTY Sbjct: 1084 PVTVSVPY---TPAGQELVNAHQLVILNIDSQGKTIVVPNGRYDAKSGSAVFKTTEFSTY 1140 Query: 479 AVVENKVNFNDVASVQAWAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLIRALN 538 AV +F DV V WA I+ +AA+ I+G+ FAP +++TRA+F +L+RAL Sbjct: 1141 AVAYVTKSFADVQHV-LWAKEAIEAMAARDIIKGISDESFAPAASITRADFITLLVRALE 1199 Query: 539 LEN-STATESFGDVASTAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIARAV 597 L+ T +F DV STA+YA VA+A E GI +G + F P ++I+R +M + A+A+ Sbjct: 1200 LKGKDTNAAAFSDVQSTAYYAQAVAIAKELGIASGFEDNTFKPGSSISRQDMMVLTAKAL 1259 Query: 598 KTVNPQATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIGNAGKFNPNNTATRAEAA 657 K +T + +L ++DAA I+ D V +V G GK P + TRAEAA Sbjct: 1260 KAAGKPSTG--SGNLAPYSDAASISTYAVDSVTSLVGSGIVNGKGGKIAPTESLTRAEAA 1317 Query: 658 VIIYRTINF 666 VI+ R N Sbjct: 1318 VILSRIWNM 1326 >ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella thermoacetica ATCC 39073] Length = 368 Score = 123 bits (309), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 102/307 (33%), Positives = 146/307 (47%), Gaps = 30/307 (9%) Query: 373 LTVTIPVSQFSDAVTLTVTNEDDATVTSLTS---LKLASKVYSFDLTVGGVKTSTFKQPL 429 + ++IP ++ V +TV + S VY+F G +TF P+ Sbjct: 74 IALSIPAGALTNDVEITVKKGAGGNAAPAPPPAGYQQISAVYNF-----GPDGTTFAVPV 128 Query: 430 TIKLPLANTTGLDKELLSV-----SKVVYKTLEFHGGVVD--GDYIVEPRDTFSTYAVV- 481 T+ L A + + E L++ +KV + + VVD I+ + FS YAV Sbjct: 129 TLYLKFALPSLVKPENLALAWYDKTKVSWVAIP---AVVDVSKGLILARINHFSDYAVFV 185 Query: 482 -ENKVNFNDVASVQ-AWAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLIRALNL 539 E + F DV WA I+ +A G + GV S F P VTRAEF+ +L++AL L Sbjct: 186 KEARKAFLDVTPASFGWAQDTIETLAGAGIVAGVDGSHFEPGRAVTRAEFASLLVKALGL 245 Query: 540 ENSTATES-FGDVASTAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIARAVK 598 E ++ F DV AWYA V AA G+V G F P+ ITR E+A M+ RAV Sbjct: 246 EGKAGEKNPFKDVKGDAWYAGAVTAAASNGLVKGYEDGTFRPDNVITREELAAMLVRAVN 305 Query: 599 TVNPQATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIG-NAGKFNPNNTATRAEAA 657 AG+ F D K++ + VA AA+H LV G G F P + A+RAE A Sbjct: 306 L-----PAGEEK--LAFKDNDKVSTWARKSVAVAAAHGLVKGFEDGTFRPGDAASRAECA 358 Query: 658 VIIYRTI 664 V++YR + Sbjct: 359 VMVYRVL 365 Score = 57.8 bits (138), Expect = 9e-07, Method: Compositional matrix adjust. Identities = 44/126 (34%), Positives = 59/126 (46%), Gaps = 10/126 (7%) Query: 544 ATESFGDV--ASTAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIARAVKTVN 601 A ++F DV AS W + A GIV G S F+P +TRAE A+++ +A+ Sbjct: 188 ARKAFLDVTPASFGWAQDTIETLAGAGIVAGVDGSHFEPGRAVTRAEFASLLVKAL---- 243 Query: 602 PQATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIG-NAGKFNPNNTATRAEAAVII 660 G A F D K A V AAS+ LV G G F P+N TR E A ++ Sbjct: 244 --GLEGKAGEKNPFKDV-KGDAWYAGAVTAAASNGLVKGYEDGTFRPDNVITREELAAML 300 Query: 661 YRTINF 666 R +N Sbjct: 301 VRAVNL 306 >gb|AAD09354.1| s-layer associated multidomain endoglucanase [Thermoanaerobacterium polysaccharolyticum] Length = 1097 Score = 117 bits (294), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 69/182 (37%), Positives = 98/182 (53%), Gaps = 7/182 (3%) Query: 487 FNDVASVQAWAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLIRALNLENSTA-T 545 F D++S +WA +IQV+A+K I G F P +TRAEF ML++AL ++ S Sbjct: 913 FTDISS--SWAKNEIQVLASKNIISGYPDGTFKPDKRITRAEFVSMLVKALGIKQSIPDV 970 Query: 546 ESFGDVASTAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIARAVKT--VNPQ 603 +F DV WY V A GI +G QF P+ ITR EM M+ A++ V Sbjct: 971 PTFSDVNKGDWYYGLVEAAKSTGIASGY-GKQFKPDMQITRQEMMVMVVNALRVNKVEKF 1029 Query: 604 ATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIGNAGKF-NPNNTATRAEAAVIIYR 662 + GD S L KF D K+ KD +A S+ L+ G ++ +P+ ATRA+AA +IYR Sbjct: 1030 VSKGDVSVLGKFKDGGKVQNWAKDAMAIGVSNGLIKGTGDEYLSPDGRATRAQAAAVIYR 1089 Query: 663 TI 664 + Sbjct: 1090 ML 1091 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.129 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 758,005,174 Number of extensions: 27013246 Number of successful extensions: 86325 Number of sequences better than 1.0e-05: 146 Number of HSP's gapped: 85902 Number of HSP's successfully gapped: 190 Length of query: 667 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 535 Effective length of database: 398,844,510 Effective search space: 213381812850 Effective search space used: 213381812850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)