BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0022.p (479 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_691960.1| multidrug resistance protein [Oceanobacillus ih... 327 4e-88 ref|NP_690993.1| multidrug resistance protein [Oceanobacillus ih... 310 5e-83 ref|NP_466111.1| similar to drug-export proteins [Listeria monoc... 304 3e-81 ref|NP_472062.1| similar to drug-export proteins [Listeria innoc... 303 7e-81 ref|NP_388266.1| similar to multidrug resistance protein [Bacill... 301 2e-80 >ref|NP_691960.1| multidrug resistance protein [Oceanobacillus iheyensis HTE831] dbj|BAC12995.1| multidrug resistance protein (efflux transporter) [Oceanobacillus iheyensis HTE831] Length = 470 Score = 327 bits (838), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 175/454 (38%), Positives = 277/454 (61%), Gaps = 5/454 (1%) Query: 8 HAMKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGIV 67 M R P+++V L+ G F+A L QT+L TALP +MSDL ++AN AQWL + ++LV+GI+ Sbjct: 11 EKMSRVPILVV--LLSGAFVAILNQTLLGTALPHIMSDLELEANTAQWLQSAFMLVNGIM 68 Query: 68 IPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVFLN 127 IP+T FLIG ++TR+LF A++ +F GTLI G + F +L GRI+Q GAG+++P+ Sbjct: 69 IPITAFLIGTFTTRSLFLAAMSSFAIGTLICGIAPNFELLLTGRILQAAGAGIIMPLMQT 128 Query: 128 TIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFFVA 187 +++++P+E+ G+AMG+ LI+ APA+GP++SG++V+ WR LFF V PIAI N +A Sbjct: 129 IMMLIYPREQRGSAMGMFGLIISFAPAIGPSLSGYIVEHSPWRTLFFMVLPIAIINIGIA 188 Query: 188 YYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFIITFL 247 Y+ L+NV + +LD+ S++ STLGFG LLYGFSI G G G I Sbjct: 189 YFLLKNVTKRTFPQLDKLSIILSTLGFGGLLYGFSIAGSAGWLSNQVIISMIVGIIALAW 248 Query: 248 FVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRESX 307 F+ RQ L P+L+F VF+Y FT +I+G++ F+ M G ++LP+Y Q++ G ES Sbjct: 249 FILRQLNLKTPILEFRVFQYKIFTITTILGMVTFMTMIGAAVILPLYMQDMLGFSAFESG 308 Query: 308 XXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLMGI 367 + ++G+++DR+G R+L G I + + M LT SF+ + I Sbjct: 309 LALLAGAVLQGIMNPVTGRLFDRYGSRYLAIIGLGLIVVTTFMFGM-LTAETSFTYIATI 367 Query: 368 YALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMTITADY 427 +A+ M G+ M P+T + SLP+ + + + + +R +S ++G AL IT+M+ TA Sbjct: 368 HAIRMLGVAMAMMPVTTAGLNSLPDEFIAHGTAVNNTLRQVSGAIGTALPITIMS-TAAI 426 Query: 428 SSVSFPVNMLEGIRAAFWTLTVIAAGGFFLSFFV 461 S ++ G+ +F +I+ G L+FF+ Sbjct: 427 PSEGLQ-GLIHGVNVSFIVTAIISIVGLILAFFI 459 >ref|NP_690993.1| multidrug resistance protein [Oceanobacillus iheyensis HTE831] dbj|BAC12028.1| multidrug resistance protein [Oceanobacillus iheyensis HTE831] Length = 509 Score = 310 bits (794), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 176/485 (36%), Positives = 280/485 (57%), Gaps = 20/485 (4%) Query: 10 MKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGIVIP 69 + + P+I V +++G+F+A L QT+LN ALP M+ D+ + AN+AQ L T+++LV+GI+IP Sbjct: 13 LPKVPIIFV--MVMGSFIAILNQTLLNVALPVMIVDMNMSANQAQSLTTIFMLVNGILIP 70 Query: 70 VTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVFLNTI 129 +T FL+ K++TR LF ++ F GTL+ G + + +L+GR+IQ GAG+++P+ +N I Sbjct: 71 ITAFLMEKFTTRQLFLTAMSLFALGTLVCGLAFGYPSLLVGRVIQAAGAGIMMPLLMNVI 130 Query: 130 IMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFFVAYY 189 + +FP K G+AMG L + APA+GPT+SG VVQ++SWR+LFF V PIAI + +A++ Sbjct: 131 LSLFPINKRGSAMGYIGLAMMFAPAIGPTLSGLVVQNYSWRVLFFIVLPIAIIDIILAFF 190 Query: 190 CLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFIITFLFV 249 L NV + + K++ S+L ST+ FG LL GFS+ GE G G I+ F+ Sbjct: 191 FLRNVTKQSNPKVNYISILMSTVAFGGLLLGFSLAGEMGWKHVEVIAPLMIGAIVMVFFI 250 Query: 250 KRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRESXXX 309 + Q KL P+L+F VF++ F+ ++I V++ + M +L+PIY QN+RG P S Sbjct: 251 RMQLKLKNPILEFRVFKFSMFSLTTVISVVITMSMFAAMILVPIYLQNLRGFSPLASGLM 310 Query: 310 XXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLMGIYA 369 I+GK++DR G R L TG I A+++ L L+ + LM +Y+ Sbjct: 311 LLPGALISAFMSPITGKLFDRIGARPLAITG-LGITAITTFLFSDLSNETGYYYLMFVYS 369 Query: 370 LLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMT------- 422 L M GI +M PI + LP + + + M +R +S ++G A L+T+M+ Sbjct: 370 LRMVGISMIMMPIMTAGLNQLPQRLYSHGTAMANTLRQMSGAIGTAFLVTVMSSQTVKHM 429 Query: 423 -------ITADYSSVSFPVNMLEGIRAAFWTLTVIAAGGFFLSFFVPY---AQGSAHPQV 472 ++ + V + +EGI +F T+ A GF LSFF+ + S Sbjct: 430 DEMTQTVVSEEQLKVIENLATIEGINDSFMIATLFAIFGFILSFFIKRRYPPEESVETVS 489 Query: 473 KESRQ 477 KE ++ Sbjct: 490 KEQKR 494 >ref|NP_466111.1| similar to drug-export proteins [Listeria monocytogenes EGD-e] pir||AD1398 drug-export proteins homolog lmo2588 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00666.1| lmo2588 [Listeria monocytogenes] Length = 493 Score = 304 bits (778), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 176/488 (36%), Positives = 278/488 (56%), Gaps = 17/488 (3%) Query: 1 MEQKVVEHAMKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVY 60 +E+ V + ++V ++I+G F+A L QT+L TALP +M DL I A QWL T + Sbjct: 6 VERPVDVNGKSYSRSLLVVTMIIGAFVAILNQTLLATALPMIMDDLHITAATGQWLTTAF 65 Query: 61 LLVSGIVIPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGL 120 LL +GI+IP+T LI K S++ LF ++ FT GT+IA + F +L GRI+Q GAG+ Sbjct: 66 LLTNGIMIPITALLIEKISSKTLFITAMTVFTIGTIIASVAGSFPILLTGRIVQAAGAGI 125 Query: 121 LIPVFLNTIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIA 180 ++P+ +++FP+EK GAAMGL L++ APA+GPT+SG++V + WR+LF + PIA Sbjct: 126 MMPLLQTIFLLIFPREKRGAAMGLMGLVIAFAPAIGPTLSGWIVDSYDWRVLFLILIPIA 185 Query: 181 IANFFVAYYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXX 240 + + +A++ ++ V + TK+D S++ S++GFG+LLYGFS G G Sbjct: 186 VIDIILAFFGMKKVVKLTDTKIDFLSIVMSSIGFGALLYGFSSAGNDGWGDTTVITMLIV 245 Query: 241 GFIITFLFVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRG 300 G ++ LFV RQ + P+L+ +VF+YP F+ + I+G I+ + M G E++LP+Y Q +RG Sbjct: 246 GVVVIALFVWRQLVIDNPMLELHVFKYPVFSLSVILGSIVTMAMIGAEIVLPLYIQTIRG 305 Query: 301 LMPRESXXXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHAS 360 +S + I+G I+D+ G + L TG I + ++ M LT+ Sbjct: 306 ESALQSGLLLLPGAIIMGIMSPITGIIFDKIGAKWLTITGVT-ILTIGTIPFMFLTMDTP 364 Query: 361 FSLLMGIYALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITL 420 ++ YA+ GI M P++ M +LPN ++ + S + IR ++ S+G A+LIT+ Sbjct: 365 LWYIVVFYAVRFFGISMAMMPVSTAGMNALPNHLINHGSAVNNTIRQIAGSIGTAVLITV 424 Query: 421 MT-ITADY---------SSVSFPVNM----LEGIRAAFWTLTVIAAGGFFLSFFVPYAQG 466 +T +T D SF L+G+RAAF + AA G LS F+ + Sbjct: 425 LTNVTKDNMPGKALMATDPASFAQKAQDASLDGMRAAFMVAAIFAAIGMILSLFLKTKK- 483 Query: 467 SAHPQVKE 474 P VKE Sbjct: 484 -QEPIVKE 490 >ref|NP_472062.1| similar to drug-export proteins [Listeria innocua] pir||AG1773 drug-export protein homolog lin2733 [imported] - Listeria innocua (strain Clip11262) emb|CAC97959.1| lin2733 [Listeria innocua] Length = 493 Score = 303 bits (776), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 175/488 (35%), Positives = 278/488 (56%), Gaps = 17/488 (3%) Query: 1 MEQKVVEHAMKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVY 60 +E+ V + ++V ++I+G F+A L QT+L TALP +M DL I A QWL T + Sbjct: 6 VERPVDVNGKSYSRSLLVVTMIIGAFVAILNQTLLATALPMIMDDLHITAATGQWLTTAF 65 Query: 61 LLVSGIVIPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGL 120 LL +GI+IP+T LI K S++ LF ++ FT GT+I+ + F +L GRI+Q GAG+ Sbjct: 66 LLTNGIMIPITALLIEKISSKTLFITAMTVFTIGTIISSVAGSFPILLTGRIVQAAGAGI 125 Query: 121 LIPVFLNTIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIA 180 ++P+ +++FP+EK GAAMGL L++ APA+GPT+SG++V + WR+LF + PIA Sbjct: 126 MMPLLQTIFLLIFPREKRGAAMGLMGLVIAFAPAIGPTLSGWIVDSYDWRVLFIILIPIA 185 Query: 181 IANFFVAYYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXX 240 + + +A++ ++ V + TK+D S++ S++GFG+LLYGFS G G Sbjct: 186 VIDIILAFFGMKKVVKLTDTKIDFLSIVMSSIGFGALLYGFSSAGNDGWGDATVITTLII 245 Query: 241 GFIITFLFVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRG 300 G ++ LFV RQ + P+L+ +VF+YP F+ + I+G I+ + M G E++LP+Y Q +RG Sbjct: 246 GVVVIALFVWRQLVIENPMLELHVFKYPVFSLSVILGSIVTMAMIGAEIVLPLYIQTIRG 305 Query: 301 LMPRESXXXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHAS 360 +S + I+G I+D+ G + L TG I + ++ M LT+ Sbjct: 306 ESALQSGLLLLPGAIIMGIMSPITGIIFDKIGAKWLTITGVT-ILTIGTIPFMFLTMDTP 364 Query: 361 FSLLMGIYALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITL 420 ++ YA+ GI M P++ M +LPN ++ + S + IR ++ S+G A+LIT+ Sbjct: 365 LWYIVVFYAVRFFGISMAMMPVSTAGMNALPNHLINHGSAVNNTIRQIAGSIGTAVLITV 424 Query: 421 MT-ITADY---------SSVSFPVNM----LEGIRAAFWTLTVIAAGGFFLSFFVPYAQG 466 +T +T D SF L+G+RAAF + AA G LS F+ + Sbjct: 425 LTNVTKDNMPGKALMATDPASFAQKAQDASLDGMRAAFMVAAIFAALGMILSLFLKNKK- 483 Query: 467 SAHPQVKE 474 P VKE Sbjct: 484 -QEPIVKE 490 >ref|NP_388266.1| similar to multidrug resistance protein [Bacillus subtilis] pir||F69763 multidrug resistance protein homolog ycnB - Bacillus subtilis dbj|BAA09016.1| homologue of multidrug resistance protein B, EmrB, of E. coli [Bacillus subtilis] emb|CAB12192.1| ycnB [Bacillus subtilis subsp. subtilis str. 168] Length = 472 Score = 301 bits (772), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 176/467 (37%), Positives = 272/467 (58%), Gaps = 5/467 (1%) Query: 9 AMKRKPV---IIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSG 65 ++++KP +IV L+ G F+A L QT+L TALP +M D + AN+AQWL T ++L +G Sbjct: 4 SIEQKPFNRSVIVGILLAGAFVAILNQTLLITALPHIMRDFNVDANQAQWLTTSFMLTNG 63 Query: 66 IVIPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVF 125 I+IP+T FLI K+++RAL ++ FT GT++ F+ F +L RIIQ GAG+++P+ Sbjct: 64 ILIPITAFLIEKFTSRALLITAMSIFTAGTVVGAFAPNFPVLLTARIIQAAGAGIMMPLM 123 Query: 126 LNTIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFF 185 + +FP EK G AMG+ L++ APA+GPT+SG+ V+ SWR LF+ + P A+ + Sbjct: 124 QTVFLTIFPIEKRGQAMGMVGLVISFAPAIGPTLSGWAVEAFSWRSLFYIILPFAVIDLI 183 Query: 186 VAYYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFIIT 245 +A ++NV KT++D SV+ ST GFG LLYGFS +G G G I Sbjct: 184 LASILMKNVTTLRKTQIDILSVILSTFGFGGLLYGFSSVGSYGWSSSTVLISLLVGVIAL 243 Query: 246 FLFVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRE 305 LF+ RQ KL P+L+F VF + F+ +++G ++F ++ G E +LP+Y QNVR + + Sbjct: 244 LLFITRQMKLKKPMLEFRVFTFGVFSLTTLLGTLVFALLIGTETILPLYTQNVRDVTAFD 303 Query: 306 SXXXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLM 365 + I G+I+DR GGR L G F I ++SL M LT H S + ++ Sbjct: 304 TGLMLLPGAVVMGFMSPIIGRIFDRVGGRGLAIAG-FCIIFLTSLPFMQLTDHTSLAWIV 362 Query: 366 GIYALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMTITA 425 +Y + + G +M P+T + +LP ++ + + M IR + S+G ALL+++M+ A Sbjct: 363 VLYTVRLLGTAMIMMPVTTAGINALPRHLIPHGTAMNNTIRQVGGSIGTALLVSVMSNQA 422 Query: 426 DYS-SVSFPVNMLEGIRAAFWTLTVIAAGGFFLSFFVPYAQGSAHPQ 471 ++ + + L G+ AAF VIA GF LSF + Q A Q Sbjct: 423 AHAGTTNVKHAALHGMNAAFIVAAVIALVGFLLSFTLKKPQRPAEQQ 469 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.329 0.142 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 599,847,412 Number of extensions: 23028606 Number of successful extensions: 100260 Number of sequences better than 1.0e-05: 1424 Number of HSP's gapped: 98828 Number of HSP's successfully gapped: 1431 Length of query: 479 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 350 Effective length of database: 406,989,807 Effective search space: 142446432450 Effective search space used: 142446432450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)