BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0020.p (491 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_347139.1| Carboxyl-terminal protease [Clostridium acetobu... 201 2e-50 ref|NP_783032.1| tail-specific protease [Clostridium tetani E88]... 199 2e-49 ref|NP_623549.1| Periplasmic protease [Thermoanaerobacter tengco... 199 2e-49 ref|NP_640379.1| carboxyl-terminal protease [Xanthomonas axonopo... 198 2e-49 ref|ZP_00097427.1| COG0793: Periplasmic protease [Desulfitobacte... 197 6e-49 >ref|NP_347139.1| Carboxyl-terminal protease [Clostridium acetobutylicum] pir||D96961 carboxyl-terminal protease [imported] - Clostridium acetobutylicum gb|AAK78479.1| Carboxyl-terminal protease [Clostridium acetobutylicum] Length = 403 Score = 201 bits (512), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 113/313 (36%), Positives = 182/313 (58%), Gaps = 9/313 (2%) Query: 60 LEQYNVEGIDQDTLIRGAIDGMVNTLNDPYSQYFDKDEAAAFSHAVD-LEYVGIGIRMVY 118 L +Y I L+ GA+ GM ++L DPY+ Y DK E +F+ + +YVG+G+ + Sbjct: 62 LYKYYDGKISDSVLLDGALKGMTSSLKDPYTVYMDKSETKSFNSEIQGQQYVGLGMEVQA 121 Query: 119 TAKELYIEEVVAGSPAEQAGLKRGDTILKINGVKIQDTKGDE----LSGTAGTKVSLLVS 174 ++ + V SPAE+AG+K GD I+K+NG T ++ + G GT V+L + Sbjct: 122 KDNKVIVSTVFDNSPAEKAGMKSGDVIVKVNGTDAVSTDLEKTVSMIKGKEGTSVTLTLY 181 Query: 175 R--KGVLKTFSVKRSEMASTSVTSKMLGQNIAYISIGGFTQNADEEFTAALDKMRAGGMK 232 R KG + KR ++A +V+ +ML I Y+ + F +N F +D+++ GMK Sbjct: 182 RSTKGNF-DITAKRQKVAIDTVSGEMLSNEIGYMQVSMFDENTGNNFNKKIDELKQQGMK 240 Query: 233 SLVLDLRDNTGGYMDSAYNIASQFMDKG-IMMYTSDQSGALTPVTITNGSKIGVPVVVLT 291 L+LDLR N GG + + + S F+ K +++ T D+ + G+ IG+P+VVL Sbjct: 241 GLILDLRSNPGGLLSACIQVTSNFVTKDKVIVSTIDKYNSKEEYKSKGGNYIGLPLVVLV 300 Query: 292 NEYTASASEALTGALHDNKLATVVGTRSFGKARIQSLIPVSDGAELKLTTQKYLTPSKED 351 + TASASE +GA+ D KL T++G ++FGK +Q+ ++DG +LK+T KY TP+ E+ Sbjct: 301 DGNTASASEIFSGAIRDYKLGTLIGEKTFGKGVVQAPFDLNDGTQLKITISKYYTPNGEN 360 Query: 352 FNHIGLSPDIEVQ 364 +H G+ PDIEV+ Sbjct: 361 IHHKGIKPDIEVK 373 >ref|NP_783032.1| tail-specific protease [Clostridium tetani E88] gb|AAO36969.1| tail-specific protease [Clostridium tetani E88] Length = 399 Score = 199 bits (505), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 113/311 (36%), Positives = 185/311 (59%), Gaps = 9/311 (2%) Query: 61 EQYNVEGIDQDTLIRGAIDGMVNTLNDPYSQYFDKDEAAAFSHAVDLEYVGIGIRMVYTA 120 E+Y+ E I++ L GAI G+ N+L DPY+ + K E F+ + Y G+GI++ Sbjct: 62 ERYDGE-INEKDLEEGAIKGLANSLKDPYTVFMTKKEYDDFNTQTEGNYSGVGIQLQAKD 120 Query: 121 KELYIEEVVAGSPAEQAGLKRGDTILKINGVKIQDTKGDE----LSGTAGTKVSLLVSRK 176 ++ I ++ SPA +AG+ D I K+N + + ++ ++ + G G++V L + RK Sbjct: 121 NKIVIVDIFEESPARKAGILPKDEIEKVNDIPVDGSQLEKAVSLMKGVEGSEVKLTLFRK 180 Query: 177 GVLKTFSV--KRSEMASTSVTSKMLGQNIAYISIGGFTQNADEEFTAALDKMRAGGMKSL 234 F V KRS++ +V +ML +++A + + F +N + F + LDK+++ GMK L Sbjct: 181 DK-GNFDVNLKRSKINLKTVAGQMLDEDVALVQVTMFDENTAKNFKSELDKLKSQGMKGL 239 Query: 235 VLDLRDNTGGYMDSAYNIASQFMDKG-IMMYTSDQSGALTPVTITNGSKIGVPVVVLTNE 293 VLDLR N GG +D ++ S F+ KG ++ T D+ + G+ +G+P+VVLTNE Sbjct: 240 VLDLRGNPGGVLDECVDMVSNFVPKGKTIVSTMDKYKSERKYKSKGGNYVGLPLVVLTNE 299 Query: 294 YTASASEALTGALHDNKLATVVGTRSFGKARIQSLIPVSDGAELKLTTQKYLTPSKEDFN 353 +ASASE GA+ D K+ T+VG ++FGK +Q+L +G LK+T KY TP+ E+ N Sbjct: 300 GSASASEIFAGAIRDYKIGTLVGEKTFGKGLVQTLYETGEGTALKITISKYYTPNGENIN 359 Query: 354 HIGLSPDIEVQ 364 IG+ PD+EV+ Sbjct: 360 KIGIKPDVEVK 370 >ref|NP_623549.1| Periplasmic protease [Thermoanaerobacter tengcongensis] gb|AAM25153.1| Periplasmic protease [Thermoanaerobacter tengcongensis] Length = 398 Score = 199 bits (505), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 117/319 (36%), Positives = 182/319 (57%), Gaps = 9/319 (2%) Query: 52 RINEIMQYLEQYNVEGIDQDTLIRGAIDGMVNTLNDPYSQYFDKDEAAAFSHAVDLEYVG 111 ++ E+ + LE V+ ++ TL+ GA+ GM +L DPY+ Y +K E A F Y G Sbjct: 52 KLFEVGKILESRYVDKVNPSTLLEGALKGMAGSLQDPYTVYMNKKEFAEFMTQTTGTYGG 111 Query: 112 IGIRMVYTAKELYIEEV--VAGSPAEQAGLKRGDTILKINGVKIQDTKGDE----LSGTA 165 IGI +V +E +I V + +P E+AG+K GD IL++NG K+ DE + G Sbjct: 112 IGI-IVAVDQEDHIVVVSPIKNTPGERAGIKSGDIILEVNGKKVSGKNLDEAVSMMRGPK 170 Query: 166 GTKVSLLVSRKGVLKTFSVKRSEMASTSVTSKMLGQNIAYISIGGFTQNADEEFTAALDK 225 GT+V+L + R G T ++ R + +V ML I YI I F Q+ +F AL++ Sbjct: 171 GTQVTLTIMRDGKTFTKTITREIIKLETVFDYMLPDKIGYIKITLFDQSTSSDFKKALER 230 Query: 226 MRAGGMKSLVLDLRDNTGGYMDSAYNIASQFMDKGIMMYTSDQSGALTPVTITNGSKIGV 285 ++ GMK L++DLRDN GG ++ NIA + KG+++ T + A + + G+ +G+ Sbjct: 231 LKKDGMKGLIIDLRDNPGGLLEECVNIADMLLPKGVIVTTKGR--AESQEFYSKGTGLGM 288 Query: 286 PVVVLTNEYTASASEALTGALHDNKLATVVGTRSFGKARIQSLIPVSDGAELKLTTQKYL 345 P+VVL N+ +ASASE L GA+ D K +VGT +FGK +Q+++ DG LK T +Y Sbjct: 289 PLVVLVNKGSASASEILAGAIKDRKAGVLVGTTTFGKGLVQTVVNFGDGTGLKYTIARYY 348 Query: 346 TPSKEDFNHIGLSPDIEVQ 364 TP+ D G+ P+ V+ Sbjct: 349 TPNGTDIQGKGIEPNYVVE 367 >ref|NP_640379.1| carboxyl-terminal protease [Xanthomonas axonopodis pv. citri str. 306] gb|AAM34915.1| carboxyl-terminal protease [Xanthomonas axonopodis pv. citri str. 306] Length = 508 Score = 198 bits (504), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 123/334 (36%), Positives = 190/334 (56%), Gaps = 22/334 (6%) Query: 45 QAAASTDRINEIMQYLEQYN------VEGIDQDTLIRGAIDGMVNTLNDPYSQYFDKDEA 98 +AA S ++EI +++ YN V+ ++ L+ A+ G+++ L DP+S YFDK++A Sbjct: 40 EAAVSKVPLDEIRRFVAVYNAVKQAYVDPVEDKKLMHAAVRGLLSDL-DPHSTYFDKEDA 98 Query: 99 AAFSHAVDLEYVGIGIRMVYTAKE-LYIEEVVAGSPAEQAGLKRGDTILKINGVKIQDTK 157 AF Y GIG+ ++ L + + +PA +AG++ GD I+ I+G I +K Sbjct: 99 EAFDEQATGAYDGIGVELLQQQDNTLKVIAPIDDTPAARAGIRAGDVIVAIDGKPIDASK 158 Query: 158 GDE-LSGTAGTKVSLLVSRKGVLKTFSV--KRSEMASTSVTSKMLGQNIAYISIGGFTQN 214 E L G +G+KV L + R + K F V +R + SV SK+L YI I F + Sbjct: 159 AMEPLRGESGSKVVLTIMRDKMPKPFDVTLQRETIRVASVRSKLLEPGFGYIRISTFQAD 218 Query: 215 ADEEFTAALDKMRAGG-MKSLVLDLRDNTGGYMDSAYNIASQFMDKGIMMYT------SD 267 +F L +++AGG ++ LVLDLR N GG + SA +A +DKG ++ T SD Sbjct: 219 TGADFQKNLKQLQAGGKLRGLVLDLRSNPGGLLTSAVQVADDLLDKGNIVSTRGRISISD 278 Query: 268 QSGALTPVTITNGSKIGVPVVVLTNEYTASASEALTGALHDNKLATVVGTRSFGKARIQS 327 TP + NG+ PVVVL + +ASASE L GAL DNK A ++G+R+FGK +Q+ Sbjct: 279 AKFDATPGDLLNGA----PVVVLVDAGSASASEVLAGALRDNKRARIIGSRTFGKGSVQT 334 Query: 328 LIPVSDGAELKLTTQKYLTPSKEDFNHIGLSPDI 361 ++P+ +G +KLTT +Y TPS + G+ PD+ Sbjct: 335 VLPLDNGDSVKLTTARYYTPSGKSIQASGIVPDV 368 >ref|ZP_00097427.1| COG0793: Periplasmic protease [Desulfitobacterium hafniense] Length = 555 Score = 197 bits (500), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 113/343 (32%), Positives = 189/343 (55%), Gaps = 5/343 (1%) Query: 41 TDQAQAAASTDRINEIMQYLEQYNVEGIDQDTLIRGAIDGMVNTLNDPYSQYFDKDEAAA 100 T A AAA ++++ LE V+ +D L +++ M+ L+DP++ +F + E Sbjct: 33 TVSAPAAALGSPVDDVRSLLESQYVDPVDPWVLSASSVEEMLKRLDDPHTVFFTQKEYQN 92 Query: 101 FSHAVDLEYVGIGIRMVYTAKELYIEEVVAGSPAEQAGLKRGDTILKINGVKI----QDT 156 F +++DL + GIG+ + + L I ++ GSPAE+A LK GD I ++ G + QD Sbjct: 93 FLNSMDLSFSGIGVYIELEPRGLEIVGIIPGSPAEEAKLKTGDIIAQVGGQSLAGLSQDA 152 Query: 157 KGDELSGTAGTKVSLLVSRKGVLKTFSVKRSEMASTSVTSKMLGQNIAYISIGGFTQNAD 216 + G GT + ++V R + V R + +V+ +ML ++I Y++I F + + Sbjct: 153 ATTLIKGPEGTTIDIVVLRGEERLSLKVARRAVEVPTVSGEMLNEDIGYVAIESFGETTE 212 Query: 217 EEFTAALDKMRAGGMKSLVLDLRDNTGGYMDSAYNIASQFMDKGIMMYTSDQSGALTPVT 276 E F + ++ G + V+DLR+N GGY+DSA ++A F+ + + T D+S P Sbjct: 213 ELFEQVIKELDKQGADAWVMDLRNNPGGYLDSALSLAGYFIGEQTALQTKDRSQKFEPYQ 272 Query: 277 ITNGSKI-GVPVVVLTNEYTASASEALTGALHDNKLATVVGTRSFGKARIQSLIPVSDGA 335 I PV+ LTNE +ASASE LT + D + A VVGT ++GK +QSL ++DG Sbjct: 273 AEKQEFIIAEPVIFLTNENSASASEILTAVVKDYQKAVVVGTNTYGKGSVQSLWQLTDGD 332 Query: 336 ELKLTTQKYLTPSKEDFNHIGLSPDIEVQGKTAQLITALQIAG 378 LK+T K+ +P ++ N +G+SPD+E+ + L +AG Sbjct: 333 VLKITVAKFYSPYGKEINGVGISPDVEILENDPLEMAVLMLAG 375 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.129 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 642,863,727 Number of extensions: 26122540 Number of successful extensions: 64179 Number of sequences better than 1.0e-05: 831 Number of HSP's gapped: 62864 Number of HSP's successfully gapped: 832 Length of query: 491 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 361 Effective length of database: 404,274,708 Effective search space: 145943169588 Effective search space used: 145943169588 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 128 (53.9 bits)