BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0008.p (324 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAE11279.1| YoxA protein [Bacillus amyloliquefaciens] 176 5e-43 ref|NP_389718.1| similar to hypothetical proteins from B. subtil... 170 4e-41 ref|NP_347334.1| Galactose mutarotase related enzyme [Clostridiu... 110 5e-23 gb|EAC76017.1| unknown [environmental sequence] 83 8e-15 ref|ZP_00119463.1| COG2017: Galactose mutarotase and related enz... 68 3e-10 >emb|CAE11279.1| YoxA protein [Bacillus amyloliquefaciens] Length = 324 Score = 176 bits (447), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 104/296 (35%), Positives = 161/296 (54%), Gaps = 9/296 (3%) Query: 21 ELEVTLLPRLGNNVISLWDRKQNREILRKPEESDLSYYLQKPYHFGMPLLIPPGRIRQGQ 80 + E ++P G+NVISL D++ +LR+PE ++ + KP +G+P+L PP RI G Sbjct: 22 QAECIIVPEWGSNVISLRDKRTGMRLLREPETAE--QFRAKPMLYGIPVLFPPNRITDGT 79 Query: 81 FQFEGRTYQFEQNTAGGH-HIHGLHRTQAWCVSDIEEDEEGCAVTTEFNTADDPRWMEQF 139 F+F+GR YQFE N H HIHG Q W + +E+ +G V TE + + PR +QF Sbjct: 80 FRFDGRAYQFEINEEDKHNHIHGFLHNQHWKAAAAKEEGDGMTVETEIDLSKIPRVHKQF 139 Query: 140 PVPLKLEMTFRLQDNRFRQTLKITHQGTARVPFGIGYHTWFMIDGEPGRWKLQLPAENVY 199 P + + MT+ L+DN F Q I ++GT P+G+GYH+ F+ + E R +L L A+ ++ Sbjct: 140 PHDVVVRMTYTLKDNVFTQRALIINKGTEAFPWGLGYHSTFVFNEE--RSRLFLTADRMW 197 Query: 200 ELNDELLTSGNLLPLGELETXXXXXXXXXXXXDTVLRIGDQQPA--EAVLAR-DDGYVLR 256 ELN+ + +G LL + + E D V + EAV+ D+ + Sbjct: 198 ELNERFVPTGRLLDVPDKEAFRHGMSLKNKQFDDVFLSSGRNGGNNEAVIYHPDEQLSVL 257 Query: 257 YSADQEYFRHWVVYTNGTADQYLCIEPYTWLPDGPNLGKDDAFTGIITLEPRQTLA 312 Y AD F+HWVVY ++ +EPYT + D NL + + TG+ L+P + A Sbjct: 258 YQADTS-FKHWVVYNGDGKQGFVSLEPYTSVTDAFNLNQPASLTGLQILKPGEEAA 312 >ref|NP_389718.1| similar to hypothetical proteins from B. subtilis [Bacillus subtilis] sp|P39840|YOXA_BACSU Hypothetical protein yoxA pir||A69930 conserved hypothetical protein yoxA - Bacillus subtilis emb|CAB13719.1| yoxA [Bacillus subtilis subsp. subtilis str. 168] Length = 325 Score = 170 bits (431), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 98/294 (33%), Positives = 155/294 (52%), Gaps = 10/294 (3%) Query: 19 SRELEVTLLPRLGNNVISLWDRKQNREILRKPEESDLSYYLQKPYHFGMPLLIPPGRIRQ 78 + LE+ ++P G+NVISL D+ N ++LR+PE ++ + P +G+P+L PP RI Sbjct: 20 NEHLEMIVVPEWGSNVISLVDKTTNVQLLREPETAE--SFHDTPTLYGIPILFPPNRISD 77 Query: 79 GQFQFEGRTYQFEQNTAGGH-HIHGLHRTQAWCVSDIEEDEEGCAVTTEFNTADDPRWME 137 G F F GRTY F+ N H H+HG + W V ++ +EG V TE + ++ P + Sbjct: 78 GTFSFRGRTYHFDINEKDKHNHLHGFLYHEKWNVVTTKQTDEGVIVETEIDLSELPHVQK 137 Query: 138 QFPVPLKLEMTFRLQDNRFRQTLKITHQGTARVPFGIGYHTWFMIDGEPGRWKLQLPAEN 197 QFP + MT+ +++N + + ++G P+GIGYHT F+ E + L A+ Sbjct: 138 QFPHHAVVRMTYTIKENTLFKHATVMNKGKEAFPWGIGYHTTFIFPAESSLFSLT--ADQ 195 Query: 198 VYELNDELLTSGNLLPLGELETXXXXXXXXXXXXDTVLRIGDQQPA---EAVLARDDGYV 254 +EL++ LL +G L+ + E D V Q+ +AV+ ++ Sbjct: 196 QWELDERLLPTGKLMDVPYKEALHEGMDLRHKQLDDVFLSSYQKRGGENQAVIYHQHAHI 255 Query: 255 -LRYSADQEYFRHWVVYTNGTADQYLCIEPYTWLPDGPNLGKDDAFTGIITLEP 307 + Y AD++ F+HWVVY YLC EPYTW+ + NL + TG+ LEP Sbjct: 256 SIIYKADEQ-FKHWVVYNADGKQGYLCPEPYTWVTNAVNLDLPSSLTGLQVLEP 308 >ref|NP_347334.1| Galactose mutarotase related enzyme [Clostridium acetobutylicum] pir||G96985 galactose mutarotase related enzyme [imported] - Clostridium acetobutylicum gb|AAK78674.1| Galactose mutarotase related enzyme [Clostridium acetobutylicum] Length = 340 Score = 110 bits (274), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 74/303 (24%), Positives = 140/303 (46%), Gaps = 17/303 (5%) Query: 23 EVTLLPRLGNNVISLWDRKQNREILRKPEESDLSY--YLQKPYHFGMPLLIPPGRIRQGQ 80 E ++P +G NV+ L D + ILR P++ DL + + +P +G+P+L PP RI G Sbjct: 28 EALIIPDVGGNVVELKDTNKGVTILRTPKK-DLKFEDFKNRPQVYGLPVLFPPNRIDDGT 86 Query: 81 FQFEGRTYQFEQNTA-GGHHIHGLHRTQAWCVSDIEEDEEGCAVTTEFNTADDPRWMEQF 139 F+ +TY+F N A ++IHG + W V + D++ V F+ + + F Sbjct: 87 FKLGDKTYKFPINEAKNNNYIHGFIKNSKWTVHKKKIDQDKALVEVVFDFTKENEAYKYF 146 Query: 140 PVPLKLEMTFRLQDNRFRQTLKITHQGTARVPFGIGYHTWF---MIDG-EPGRWKLQLPA 195 + ++++ L +QT + + + +P +GYH+ F I+G E ++++ Sbjct: 147 SHEFQFKLSYELSSKGLKQTTSVVNLSSEEMPLSVGYHSAFNVPFIEGSEDSNCRVKISI 206 Query: 196 ENVYELNDELLTSG-NLLPLGE----LETXXXXXXXXXXXXDTVLRI---GDQQPAEAVL 247 + ++ + L +G + P GE LE ++ I G + Sbjct: 207 DKFWKQDSRNLPTGESFAPTGEQKEYLENGVAVASHPIESLFSLKDIDVNGKTFRGACIE 266 Query: 248 ARDDGYVLRYSADQEYFRHWVVYTNGTADQYLCIEPYTWLPDGPNLGKDDAFTGIITLEP 307 + Y EY ++ V++ + +Y CIEP T + + PN+ D + +G TL+P Sbjct: 267 DASKNTRVVYEMSSEY-KYLVIWNDMGDKKYACIEPQTSIINSPNVKLDRSVSGFKTLKP 325 Query: 308 RQT 310 ++ Sbjct: 326 NES 328 >gb|EAC76017.1| unknown [environmental sequence] Length = 154 Score = 83.2 bits (204), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 49/145 (33%), Positives = 73/145 (50%), Gaps = 2/145 (1%) Query: 24 VTLLPRLGNNVISLWDRKQNREILRKPEESDLSYYLQKPYHFGMPLLIP-PGRIRQGQFQ 82 T+LP +GNN IS R REI D +P +G+P+L P P RI G+F+ Sbjct: 10 ATVLPSVGNNCISYKARIDGREIELLFSAPDAETLQGRPSGYGIPILFPWPNRIEYGKFE 69 Query: 83 FEGRTYQFEQNTAGGHHIHGLHRTQAWCVSDI-EEDEEGCAVTTEFNTADDPRWMEQFPV 141 FEGR + + G H +HG + W VS + +G VT F ++D ++P Sbjct: 70 FEGRQVELKTPEEGAHLLHGYVLDRPWKVSGSGASNADGAWVTCRFQSSDFLEIGSEWPF 129 Query: 142 PLKLEMTFRLQDNRFRQTLKITHQG 166 PL++E T+RL+D + T+ G Sbjct: 130 PLEVEGTYRLKDGALSVEVTGTNTG 154 >ref|ZP_00119463.1| COG2017: Galactose mutarotase and related enzymes [Cytophaga hutchinsonii] Length = 314 Score = 67.8 bits (164), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 47/191 (24%), Positives = 82/191 (42%), Gaps = 7/191 (3%) Query: 25 TLLPRLGNNVISLWDRKQN---REILRKPEESDLSYYLQKPYHFGMPLLIPPGRIRQGQF 81 +++P G N+ L +K N I + +LS + Y G L RI +G + Sbjct: 27 SIIPSFGGNIHELVLQKHNILHDVIYTNKNKEELSGFYSNLYR-GAKLNPFSNRIDKGSY 85 Query: 82 QFEGRTYQFEQNTAGGHHIHGLHRTQAWCVSDIEEDEEGCAVTTEFNTADDPRWMEQFPV 141 FE +Y N AG H +HG + + ++ + E +T + P+ FP Sbjct: 86 IFENISYSIPLNDAGIHALHGFVWNKPFSITASKTSFEFATLTLSYT---HPKDSADFPF 142 Query: 142 PLKLEMTFRLQDNRFRQTLKITHQGTARVPFGIGYHTWFMIDGEPGRWKLQLPAENVYEL 201 L +T++L T +IT+ +P G G+H + + KLQ+P+ E Sbjct: 143 SYTLLVTYKLCIEGLMVTTQITNTSPTAIPMGDGWHPYLTLGSSINTLKLQIPSVQKIET 202 Query: 202 NDELLTSGNLL 212 D L+ +G ++ Sbjct: 203 TDTLIPTGKII 213 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.139 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 508,126,892 Number of extensions: 22042257 Number of successful extensions: 33447 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 33462 Number of HSP's successfully gapped: 14 Length of query: 324 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 198 Effective length of database: 415,135,104 Effective search space: 82196750592 Effective search space used: 82196750592 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)