BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0003.p (250 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00087722.1| COG0109: Polyprenyltransferase (cytochrome ox... 156 5e-37 gb|AAB40184.1| cytochrome o ubiquinol oxidase C subunit [Escheri... 155 6e-37 sp|Q04444|COXX_BACPF Protoheme IX farnesyltransferase (Heme O sy... 155 8e-37 ref|NP_668348.1| protoheme IX farnesyltransferase (haeme O biosy... 155 9e-37 ref|NP_286170.1| protoheme IX farnesyltransferase (haeme O biosy... 155 1e-36 >ref|ZP_00087722.1| COG0109: Polyprenyltransferase (cytochrome oxidase assembly factor) [Pseudomonas fluorescens PfO-1] Length = 295 Score = 156 bits (394), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 78/166 (46%), Positives = 110/166 (66%) Query: 28 RDFITVTKPGIIRSNLIAAFAGYWVASGWDVQYGRLILTLLGTMLVMASACVFNNYFDRD 87 + FI +TKPGII N+++ G+++AS V + ++GT LV+AS CVFNN DRD Sbjct: 4 KHFIQITKPGIIFGNVLSVAGGFFLASKGHVDLAVFLAAMIGTSLVVASGCVFNNCIDRD 63 Query: 88 LDMKMERTRERGLPTGRLKPQTVLIYAIGLGIAGLAVLFAFCGVLAGIFGIVGMFVYVVV 147 +D+KMERT+ R L G + + L+YA LG+AG+A+L+ LA +F ++G +YV Sbjct: 64 IDLKMERTKNRVLVQGLISLKLALVYATVLGVAGVALLYKVANPLAALFAVIGFIIYVGF 123 Query: 148 YTLWLKRTSTWSTSVGAISGAMPPVIGYVAVTGAVDLGAWLLFAML 193 Y+L+LKR S T VG++SGAMPPVIGYVAVT + D+ A L M Sbjct: 124 YSLYLKRKSVHGTLVGSLSGAMPPVIGYVAVTNSFDMAALTLLVMF 169 >gb|AAB40184.1| cytochrome o ubiquinol oxidase C subunit [Escherichia coli] Length = 311 Score = 155 bits (393), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 83/167 (49%), Positives = 110/167 (65%) Query: 27 WRDFITVTKPGIIRSNLIAAFAGYWVASGWDVQYGRLILTLLGTMLVMASACVFNNYFDR 86 ++ ++ VTKPGII NLI+ G+ +AS + Y I TL+G LV+AS CVFNNY DR Sbjct: 18 FKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLVGVSLVVASGCVFNNYIDR 77 Query: 87 DLDMKMERTRERGLPTGRLKPQTVLIYAIGLGIAGLAVLFAFCGVLAGIFGIVGMFVYVV 146 D+D KMERT+ R L G + P L+YA LGIAG +L+ LA G++G VYV Sbjct: 78 DIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLWFGANPLACWLGVMGFVVYVG 137 Query: 147 VYTLWLKRTSTWSTSVGAISGAMPPVIGYVAVTGAVDLGAWLLFAML 193 VY+L++KR S + T +G++SGA PPVIGY AVTG D GA +L A+ Sbjct: 138 VYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDSGAAILLAIF 184 >sp|Q04444|COXX_BACPF Protoheme IX farnesyltransferase (Heme O synthase) pir||B45335 probable heme A farnesyltransferase (EC 2.5.1.-) - Bacillus firmus gb|AAA22363.1| CtaB protein Length = 312 Score = 155 bits (392), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 81/187 (43%), Positives = 117/187 (62%), Gaps = 8/187 (4%) Query: 23 EGAGWRDFITVTKPGIIRSNLIAAFAGYWVA-----SGWDVQYGRLILTLLGTMLVMASA 77 + W+D++ + K GI+ SNLI FAG ++A + + + +I LLG LVMA Sbjct: 26 QQKSWKDYLVLAKQGIVTSNLITTFAGIYLAIVYTGTVFTMHLDTMIFALLGAALVMAGG 85 Query: 78 CVFNNYFDRDLDMKMERTRERGLPTGRLKPQTVLIYAIGLGIAGLAVLF-AFCGVLAGIF 136 C NNY DRD+D MERT+ER TGR + VL+ +GL A L ++F A A + Sbjct: 86 CTLNNYIDRDIDHLMERTKERPTVTGRFSAKHVLL--VGLAQAALGIIFLALTTPTAAVI 143 Query: 137 GIVGMFVYVVVYTLWLKRTSTWSTSVGAISGAMPPVIGYVAVTGAVDLGAWLLFAMLXXX 196 G++G+F+YVV+YT+W KRT+T +T VG+ SGA+PP+IG+ A+ G + L AWLLF ++ Sbjct: 144 GLIGLFIYVVLYTMWTKRTTTLNTIVGSFSGAVPPLIGWAAIDGGLHLYAWLLFFIMFLW 203 Query: 197 XPAAFLG 203 P FL Sbjct: 204 QPPHFLA 210 >ref|NP_668348.1| protoheme IX farnesyltransferase (haeme O biosynthesis) [Yersinia pestis KIM] ref|NP_992151.1| protoheme IX farnesyltransferase [Yersinia pestis biovar Mediaevails str. 91001] gb|AAM84599.1| protoheme IX farnesyltransferase (haeme O biosynthesis) [Yersinia pestis KIM] gb|AAS61028.1| protoheme IX farnesyltransferase [Yersinia pestis biovar Mediaevails str. 91001] Length = 302 Score = 155 bits (392), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 81/163 (49%), Positives = 109/163 (66%) Query: 28 RDFITVTKPGIIRSNLIAAFAGYWVASGWDVQYGRLILTLLGTMLVMASACVFNNYFDRD 87 + ++ VTKPGII NLI+ G+ +AS D+ Y + TLLG LV+AS CVFNNY DRD Sbjct: 10 KQYLQVTKPGIIFGNLISVIGGFLLASKGDIDYPLFLSTLLGVSLVVASGCVFNNYIDRD 69 Query: 88 LDMKMERTRERGLPTGRLKPQTVLIYAIGLGIAGLAVLFAFCGVLAGIFGIVGMFVYVVV 147 +D MERT+ R L G + P+ LIYA LGIAG+ +L+ LA + ++G +YV V Sbjct: 70 IDKIMERTKNRVLVKGLIDPKVSLIYASVLGIAGMLLLYVAANALAMMLAVIGFVIYVGV 129 Query: 148 YTLWLKRTSTWSTSVGAISGAMPPVIGYVAVTGAVDLGAWLLF 190 Y+L++KR S + T +G++SGA PPVIGY AVTG D GA +L Sbjct: 130 YSLYMKRKSVYGTLIGSLSGAAPPVIGYCAVTGQFDTGALILL 172 >ref|NP_286170.1| protoheme IX farnesyltransferase (haeme O biosynthesis) [Escherichia coli O157:H7 EDL933] ref|NP_308509.1| protoheme IX farnesyltransferase [Escherichia coli O157:H7] ref|NP_414962.1| protoheme IX farnesyltransferase [Escherichia coli K12] ref|NP_752473.1| Protoheme IX farnesyltransferase [Escherichia coli CFT073] sp|P18404|CYOE_ECOLI Protoheme IX farnesyltransferase (Heme O synthase) pir||E42226 heme O synthase - Escherichia coli (strain K-12) pir||B90689 Protoheme IX farnesyltransferase (EC 2.5.1.-) [similarity] - Escherichia coli (strain O157:H7, substrain RIMD 0509952) pir||F85539 Protoheme IX farnesyltransferase (EC 2.5.1.-) - Escherichia coli (strain O157:H7, substrain EDL933) gb|AAA23635.1| cytochrome o ubiquinol oxidase C subunit gb|AAC73531.1| protoheme IX farnesyltransferase (haeme O biosynthesis) [Escherichia coli K12] gb|AAG54778.1| protoheme IX farnesyltransferase (haeme O biosynthesis) [Escherichia coli O157:H7 EDL933] dbj|BAB33905.1| protoheme IX farnesyltransferase [Escherichia coli O157:H7] gb|AAN79017.1| Protoheme IX farnesyltransferase [Escherichia coli CFT073] Length = 296 Score = 155 bits (391), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 83/167 (49%), Positives = 110/167 (65%) Query: 27 WRDFITVTKPGIIRSNLIAAFAGYWVASGWDVQYGRLILTLLGTMLVMASACVFNNYFDR 86 ++ ++ VTKPGII NLI+ G+ +AS + Y I TL+G LV+AS CVFNNY DR Sbjct: 3 FKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLVGVSLVVASGCVFNNYIDR 62 Query: 87 DLDMKMERTRERGLPTGRLKPQTVLIYAIGLGIAGLAVLFAFCGVLAGIFGIVGMFVYVV 146 D+D KMERT+ R L G + P L+YA LGIAG +L+ LA G++G VYV Sbjct: 63 DIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLWFGANPLACWLGVMGFVVYVG 122 Query: 147 VYTLWLKRTSTWSTSVGAISGAMPPVIGYVAVTGAVDLGAWLLFAML 193 VY+L++KR S + T +G++SGA PPVIGY AVTG D GA +L A+ Sbjct: 123 VYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDSGAAILLAIF 169 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.141 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 325,215,746 Number of extensions: 12276912 Number of successful extensions: 45060 Number of sequences better than 1.0e-05: 657 Number of HSP's gapped: 44861 Number of HSP's successfully gapped: 657 Length of query: 250 Length of database: 757,237,578 Length adjustment: 123 Effective length of query: 127 Effective length of database: 423,280,401 Effective search space: 53756610927 Effective search space used: 53756610927 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 124 (52.4 bits)