BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0419.0002.p (356 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00019492.1| hypothetical protein [Chloroflexus aurantiacus] 174 4e-42 ref|NP_295799.1| conserved hypothetical protein [Deinococcus rad... 155 2e-36 ref|NP_693586.1| hypothetical protein OB2665 [Oceanobacillus ihe... 145 1e-33 ref|ZP_00053504.1| COG0491: Zn-dependent hydrolases, including g... 134 2e-30 ref|ZP_00152860.1| hypothetical protein Daro109801 [Dechloromona... 130 3e-29 >ref|ZP_00019492.1| hypothetical protein [Chloroflexus aurantiacus] Length = 321 Score = 174 bits (440), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 107/306 (34%), Positives = 152/306 (49%), Gaps = 3/306 (0%) Query: 20 MTTWEGGIIQFSVPMAPPLRQVNSYILPDRNGQLTVIDPGPHTPEAEAAWEAVLQELDCS 79 +TT + Q +P+ L VN Y+L D G TV+D G HTP EAAW+ L+ Sbjct: 2 LTTILPDVYQLRLPLPFALNHVNVYLLRDPQGW-TVVDTGLHTPAGEAAWQEAWTALNLD 60 Query: 80 WSTIRDIVVTHHHPDHYGLAGWLQARSGGKVWMSERAHEEARLTWGAEGMLNEALPLFFL 139 + I IV+TH HPDH+G+AGWL SG +V ++ R E A+ W ++ FL Sbjct: 61 PAQIHTIVLTHFHPDHFGMAGWLHHHSGARVLLAPREIELAQEVWVQRADHDDPSLRHFL 120 Query: 140 RYGMPEDWVQGIREHLESFLPQVTPQPEVAYINAAEPFIMGNRKWKPLVTGGHAPGHLSF 199 R+GMP D V + + P P + + MG R + + GH+ G L F Sbjct: 121 RHGMPADLVDKVVGAIAGLRAATRPHPPLTPLPPGSQVTMGGRSFVAIHAPGHSDGQLIF 180 Query: 200 YHGGXXXGLILCGDAVLPQISPNVSLQPGSDPQPLRTFLEGLRELRSLRVTRAFPGHREP 259 Y L L GD VL +I+P++ + P S+P PLR +L L EL +L+V A PGH +P Sbjct: 181 Y--AADERLALVGDQVLLKITPHIGVWPESEPDPLRKYLASLAELATLKVDLALPGHGKP 238 Query: 260 FTGFTERADSLLRHHEERLEQXXXXXXXXXXXXXXVCEALFRSRVSSAHQMRFAMSEALA 319 T + R L HH ERL V + +F + H+ RFA++E +A Sbjct: 239 ITEWKTRISELQVHHHERLAAMLAAVRNGADTAFAVAQRVFDVERFTPHEARFAIAETIA 298 Query: 320 HLAELV 325 HL +V Sbjct: 299 HLEMMV 304 >ref|NP_295799.1| conserved hypothetical protein [Deinococcus radiodurans] pir||F75319 conserved hypothetical protein - Deinococcus radiodurans (strain R1) gb|AAF11625.1| conserved hypothetical protein [Deinococcus radiodurans] Length = 347 Score = 155 bits (391), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 109/304 (35%), Positives = 151/304 (49%), Gaps = 14/304 (4%) Query: 25 GGIIQFSVPMAPPLRQVNSYILPDRNGQLTVIDPGPHTPEAEAAWEAVLQELDCSWSTIR 84 G + VP+ P++ V I G LT+ID TPEA AA E L +L WS I Sbjct: 33 GSLHALQVPIPYPMKTVTVLIDAPARGPLTLIDCALDTPEARAAIEDGLAQLGRHWSDID 92 Query: 85 DIVVTHHHPDHYGLAGWLQARSGGKVWMSERAHEEARLTWGAEGMLNEALP---LFFLRY 141 ++VTHHHPDHYGLAG ++ RSG +V M + W ML+E LP + Sbjct: 93 RLIVTHHHPDHYGLAGVIEERSGARVQMLDVEIGRGERYW---HMLSEWLPGHRKHMRDH 149 Query: 142 GMPEDWVQGIREHLESFLPQVTPQPEVAYINAAEPFIMGNRKWKPLVTGGHAPGHLSFYH 201 G+PE V+GIR + P+V P V + + + +W+ L GHA GHL ++ Sbjct: 150 GLPEASVEGIR----AENPRVHPASRVEPLREGQSVSLAGHEWEVLWLPGHADGHLGLWN 205 Query: 202 GGXXXGLILCGDAVLPQISPNVSLQPGSDPQPLRTFLEGLRELRSLRVTRAFPGHREP-F 260 L++ GDA+LP+ISPN+ L + P PL +L+ L +L +L RA GH P Sbjct: 206 --EEESLLIAGDAILPRISPNIGLYAYTRPDPLGDYLQTLGKLEALNPRRAVVGHHGPVM 263 Query: 261 TGFTERADSLLRHHEERLEQXXXXXXXXXXXXXXVCEALFRSRVSSAHQMRFAMSEALAH 320 G RA L HH ERL+ + A+F + + RFA++E LAH Sbjct: 264 EGVQARARELRGHHHERLDFIGAEARREGRSAYDLSLAMFPRDLDEGGR-RFALAETLAH 322 Query: 321 LAEL 324 L L Sbjct: 323 LEHL 326 >ref|NP_693586.1| hypothetical protein OB2665 [Oceanobacillus iheyensis HTE831] dbj|BAC14621.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 313 Score = 145 bits (367), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 95/321 (29%), Positives = 151/321 (47%), Gaps = 15/321 (4%) Query: 26 GIIQFSVPMAPPLRQVNSYILPDRNGQLTVIDPGPHTPEAEAAWEAVLQELDCSWSTIRD 85 GI + ++ + L VN +I NG + V+D G H + W L+ + D Sbjct: 6 GIKKVTLDLPFRLNHVNCFIGETDNG-VKVMDTGLHRNQTVQRWNEELE-----GKRVSD 59 Query: 86 IVVTHHHPDHYGLAGWLQARSGGKVWMSERAHEEARLTWGAEGMLNEALPLFFLRYGMPE 145 I++TH+HPDH+G AG LQ +G VWM+E A +W E + L + G+P+ Sbjct: 60 IIITHYHPDHFGYAGGLQQLTGANVWMTETDERAAFSSW--EKPFLQQLKDNYKLAGIPK 117 Query: 146 DWVQGIREHLESFLPQVTPQPEV-AYINAAEPFIMGNRKWKPLVTGGHAPGHLSFYHGGX 204 + + ++ SF P VTP P V Y+ E G +++ + T GH+ G ++ Y Sbjct: 118 EIATELADNTSSFYPYVTPYPIVHDYLREGEQIQFGTYEYEIICTPGHSDGLVTLY--NP 175 Query: 205 XXGLILCGDAVLPQISPNVSLQPGSDPQPLRTFLEGLRELRSLRVTRAFPGHREPFTGFT 264 ++L D +LP+I+PN+S + PL+ +L L +++ L+V P H PF Sbjct: 176 EKNVLLSTDHILPKITPNISYWFHGEENPLKNYLASLEKIKKLQVEWVIPSHGNPFLDAN 235 Query: 265 ERADSLLRHHEERLEQXXXXXXXXXXXXXXVCEALFRSRVSSAHQMRFAMSEALAHLAEL 324 +R + +HHE+RL E LF ++ S H RFA+ E +AHL L Sbjct: 236 KRIQEIKKHHEDRL-SFLLEEIKVETTVYEATEKLFPKKM-SVHDSRFAVGETIAHLEYL 293 Query: 325 VRRERAEVTGPEPGGVTMFAA 345 R E + GV + Sbjct: 294 --RIAGECSKELTNGVYQYKT 312 >ref|ZP_00053504.1| COG0491: Zn-dependent hydrolases, including glyoxylases [Magnetospirillum magnetotacticum] Length = 348 Score = 134 bits (338), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 94/305 (30%), Positives = 146/305 (47%), Gaps = 10/305 (3%) Query: 26 GIIQFSVPMAPPLRQVNSYILPDRNGQLTVIDPGPHTPEAEAAWEAVLQELDCSWSTIRD 85 G+ +P+ L +N ++L D G + ++D G +A W+ + + + Sbjct: 25 GVRWIRMPLPFALDHINLWVLDDGEG-VVLVDTGLGNDVTKALWDEIFAG-PLAGKAVNR 82 Query: 86 IVVTHHHPDHYGLAGWLQARSGGKVWMSERAHEEARLTWGAEGMLNEALPL-FFLRYGMP 144 ++ TH HPDH GL+GWL + G + R R+ W + A L ++ R G+ Sbjct: 83 LIATHFHPDHMGLSGWLCEKLGVDLTAMMREWLFGRMLWLEDSADFVANQLHYYTRIGLD 142 Query: 145 EDWVQGIREHLESFLPQVTPQP-EVAYINAAEPFIMGNRKWKPLVTGGHAPGHLSFYHGG 203 + ++G++E ++ ++ P V I + +G R W+ + GGH+P H Y Sbjct: 143 AEQLEGVKERRNTYRSRIDVIPVRVIGIRHGDEMTIGGRIWRVIEGGGHSPEHACLY--C 200 Query: 204 XXXGLILCGDAVLPQISPNVSLQPGS-DPQPLRTFLEGLRELRSL-RVTRAFPGHREPFT 261 GL++ GD VLP+ISP V + P + +PL FL+ L LR+L T P H PF Sbjct: 201 EEAGLLISGDQVLPRISPIVGVWPQQPESEPLSLFLDALDRLRALPEDTFVLPSHGLPFQ 260 Query: 262 GFTERADSLLRHHEERLEQXXXXXXXXXXXXXXVCEALFRSRVSSAHQMRFAMSEALAHL 321 G R D L HH ERLE+ V + LF +R AHQ+ FA E LAHL Sbjct: 261 GLHTRIDDLKAHHAERLEK-TLGACSEAVTAVQVLKVLF-TRPLDAHQLGFATGETLAHL 318 Query: 322 AELVR 326 L++ Sbjct: 319 HHLMK 323 >ref|ZP_00152860.1| hypothetical protein Daro109801 [Dechloromonas aromatica RCB] Length = 341 Score = 130 bits (328), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 98/314 (31%), Positives = 149/314 (47%), Gaps = 22/314 (7%) Query: 26 GIIQFSVPMAPPLRQVNSYILPDRNGQLTVIDPGPHTPEAEAAWEAVLQELDCSWSTIRD 85 G+ +P+ L +N ++L D G T+ID G A W +L LD + Sbjct: 22 GVYWLRMPLPFQLDHINLWLLRDGEG-WTIIDTGFPDDAVRAMWTQILDWLD---GPVTR 77 Query: 86 IVVTHHHPDHYGLAGWLQARSGGKVWMSERAHEEARLTW------GAEGMLNEALPLFFL 139 ++VTH HPDH GLA WL ++G + M+ A + W GA M+ + F Sbjct: 78 LIVTHFHPDHLGLATWLMEKTGAPLSMTTGEFLTAHVVWHEVGGHGARFMVEQ-----FR 132 Query: 140 RYGMPEDWVQGIREHLESFLPQVTPQPEVAYINAAEPFI-MGNRKWKPLVTGGHAPGHLS 198 ++G+ + + + + V PE + A + + +KW+ L+ GHAP HL+ Sbjct: 133 QHGLDAEHLAKFEQRGSGYRRAVPALPEYYHRLMAGGIVRIDGKKWQILIGHGHAPEHLA 192 Query: 199 FYHGGXXXGLILCGDAVLPQISPNVSLQPGS-DPQPLRTFLEGLREL-RSLRV-TRAFPG 255 Y G+++ GD +LP+IS NVS+ + + LR +L+ L E+ R + T P Sbjct: 193 LY--CAELGVLISGDMLLPRISTNVSVFAATPEADALRWYLDSLDEMARDIPAETLVLPS 250 Query: 256 HREPFTGFTERADSLLRHHEERLEQXXXXXXXXXXXXXXVCEALFRSRVSSAHQMRFAMS 315 H PFTG ER +L HHEERL+ + + LF +R HQ FAM Sbjct: 251 HGLPFTGVQERVQALHAHHEERLQALENSCEQAPQSAAELLDVLF-NRALDTHQTMFAMG 309 Query: 316 EALAHLAELVRRER 329 EA+AHL L + R Sbjct: 310 EAIAHLNYLEQAGR 323 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.135 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 534,480,355 Number of extensions: 22167928 Number of successful extensions: 48321 Number of sequences better than 1.0e-05: 85 Number of HSP's gapped: 48140 Number of HSP's successfully gapped: 85 Length of query: 356 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 229 Effective length of database: 412,420,005 Effective search space: 94444181145 Effective search space used: 94444181145 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)