BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0417.0021.p (115 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_390573.1| similar to spore coat protein [Bacillus subtili... 191 4e-48 ref|NP_347251.1| Spore coat protein F [Clostridium acetobutylicu... 86 2e-16 ref|NP_241609.1| spore coat protein [Bacillus halodurans] >gi|25... 73 2e-12 ref|NP_390576.1| similar to spore coat protein [Bacillus subtili... 72 4e-12 >ref|NP_390573.1| similar to spore coat protein [Bacillus subtilis] pir||F69970 spore coat protein homolog yraF - Bacillus subtilis gb|AAB80875.1| hypothetical spore coat protein [Bacillus subtilis] emb|CAA63473.1| spore coat protein F [Bacillus subtilis] emb|CAB14637.1| yraF [Bacillus subtilis subsp. subtilis str. 168] Length = 122 Score = 191 bits (485), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 87/109 (79%), Positives = 99/109 (90%) Query: 1 MPEMADMTFATDFLLRAKEGVRNLSIALTETASPDVRALLHNHLKQGIAMHQEITELMMR 60 +PE+AD TFA DFL+RAKEGVRN ++ALTETASPDVRALL L QGIAMHQEITELM+ Sbjct: 14 VPELADTTFAMDFLIRAKEGVRNTAVALTETASPDVRALLRKQLMQGIAMHQEITELMIS 73 Query: 61 KKWFHPYELNEQYQLDQLSAKNTVMIGQMNLFPDDTSRKGMFDRTPDGH 109 KKWFHPYEL+EQY+LDQLSAKNT+M+G MNLFPD+T+RKGMFDRTPD H Sbjct: 74 KKWFHPYELSEQYKLDQLSAKNTIMVGNMNLFPDETNRKGMFDRTPDEH 122 >ref|NP_347251.1| Spore coat protein F [Clostridium acetobutylicum] pir||D96975 spore coat protein F [imported] - Clostridium acetobutylicum gb|AAK78591.1| Spore coat protein F [Clostridium acetobutylicum] Length = 98 Score = 85.9 bits (211), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 37/88 (42%), Positives = 57/88 (64%) Query: 1 MPEMADMTFATDFLLRAKEGVRNLSIALTETASPDVRALLHNHLKQGIAMHQEITELMMR 60 M + D ATDFL+ K GV+N ++A+TETA+P VRA+L HL +GI H++I MM Sbjct: 11 MDVLNDHVVATDFLISTKSGVKNYAVAITETATPRVRAILRKHLDEGINCHEKILNYMMT 70 Query: 61 KKWFHPYELNEQYQLDQLSAKNTVMIGQ 88 ++HPY + EQY+ D S + ++ + + Sbjct: 71 NGYYHPYNMQEQYKTDMKSTETSLNLNR 98 >ref|NP_241609.1| spore coat protein [Bacillus halodurans] pir||G83742 spore coat protein BH0743 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04462.1| spore coat protein [Bacillus halodurans] Length = 105 Score = 73.2 bits (178), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 37/85 (43%), Positives = 57/85 (67%), Gaps = 1/85 (1%) Query: 1 MPEMADMTFATDFLLRAKEGVRNLSIALTETASPDVRALLHNHLKQGIAMHQEITELMMR 60 M M D ATD L+ AK G++N++ A++E+A+P+VRA L L Q I H ++TE MM+ Sbjct: 11 MGPMTDQVVATDMLIAAKAGIKNIATAISESATPEVRAALQQQLDQQIRFHGQMTEYMMK 70 Query: 61 KKWFHPYELNEQYQLDQLSAKNTVM 85 ++HPY + EQ ++D L+A NT + Sbjct: 71 NGYYHPYNMEEQVRVD-LAAANTAL 94 >ref|NP_390576.1| similar to spore coat protein [Bacillus subtilis] pir||D69970 spore coat protein homolog yraD - Bacillus subtilis emb|CAA63470.1| spore coat protein F [Bacillus subtilis] emb|CAB14640.1| yraD [Bacillus subtilis subsp. subtilis str. 168] Length = 99 Score = 72.0 bits (175), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 29/73 (39%), Positives = 49/73 (67%) Query: 4 MADMTFATDFLLRAKEGVRNLSIALTETASPDVRALLHNHLKQGIAMHQEITELMMRKKW 63 + D A D L+ AK GVRN ++A TE +P+V+ +L HL++ + MH++++ MM K W Sbjct: 14 LTDQIIAMDLLISAKNGVRNYAMAATEAGTPEVKEVLIRHLEEALDMHEQLSSYMMEKGW 73 Query: 64 FHPYELNEQYQLD 76 +HP+ +EQ +L+ Sbjct: 74 YHPWNPDEQVKLN 86 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 162,267,937 Number of extensions: 5192109 Number of successful extensions: 12382 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 12382 Number of HSP's successfully gapped: 4 Length of query: 115 Length of database: 757,237,578 Length adjustment: 80 Effective length of query: 35 Effective length of database: 540,029,658 Effective search space: 18901038030 Effective search space used: 18901038030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)